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A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants

A technology of polyploidy and red fruit ginseng, applied in the biological field, can solve the problems of unrealized direct organ plant excellent traits, etc., achieve the effects of shortening the cultivation utilization cycle, vigorous metabolism, and improving stress resistance

Active Publication Date: 2022-06-21
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is only one report on tissue culture of red fruit ginseng, which uses the method of explant-callus-adventitious buds, but does not realize direct organogenesis to ensure the transmission of excellent plant traits to the greatest extent.

Method used

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  • A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants
  • A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants
  • A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants

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Effect test

Embodiment 1

[0067] A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants, comprising the following steps:

[0068] 1. Acquisition of explants: Select robust plants with good growth potential and no pests and diseases, and choose the full fruits of the year.

[0069] 2. Wash the surface of the fruit in step 1 with tap water, soak it with 10% mass percentage washing powder solution for 10 minutes, shake it slightly, rinse it with running water for 30 minutes, place it on an ultra-clean workbench and treat it with 75% volume percentage ethanol solution for 30 seconds, and then Sterilize with 0.1% mercuric chloride aqueous solution by mass percentage for 30 minutes, and finally rinse with sterile water for 3 times, each time not less than 3 minutes, fully shake the vessel during the whole disinfection process.

[0070] 3. Acquisition of sterile seedlings: Cut open the fruit sterilized in step 2, take the inner seeds as material...

Embodiment 2

[0088] A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants, comprising the following steps:

[0089] 1. Acquisition of explants: Select robust plants with good growth potential and no pests and diseases, and choose the full fruits of the year.

[0090] 2. Wash the surface of the fruit in step 1 with tap water, soak it with 10% mass percentage washing powder solution for 10 minutes, shake it slightly, rinse it with running water for 30 minutes, place it on an ultra-clean workbench and treat it with 75% volume percentage ethanol solution for 30 seconds, and then Sterilize with 0.1% mercuric chloride aqueous solution by mass percentage for 30 minutes, and finally rinse with sterile water for 3 times, each time not less than 3 minutes, fully shake the vessel during the whole disinfection process.

[0091] 3. Acquisition of sterile seedlings: Cut open the fruit sterilized in step 2, take the inner seeds as material...

Embodiment 3

[0110] A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants, comprising the following steps:

[0111] 1. Acquisition of explants: Select robust plants with good growth potential and no pests and diseases, and choose the full fruits of the year.

[0112] 2. Wash the surface of the fruit in step 1 with tap water, soak it with 10% mass percentage washing powder solution for 10 minutes, shake it slightly, rinse it with running water for 30 minutes, place it on an ultra-clean workbench and treat it with 75% volume percentage ethanol solution for 30 seconds, and then Sterilize with 0.1% mercuric chloride aqueous solution by mass percentage for 30 minutes, and finally rinse with sterile water for 3 times, each time not less than 3 minutes, fully shake the vessel during the whole disinfection process.

[0113] 3. Acquisition of sterile seedlings: Cut open the fruit sterilized in step 2, take the inner seeds as material...

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Abstract

The invention provides a method for polyploid induction of red fruit ginseng and an efficient artificial seedling cultivation method for polyploid plants, comprising the following steps: (1) inoculating the sterilized seeds in medium A for aseptic germination; (2) Get sterile seedlings with leafy stem tips and soak them in sterilized colchicine solution, then transfer them to medium C after cultivating them in medium B; (3) polyploid plants are grown in medium C Proliferation and rooting can be completed in one step. The invention optimizes and adjusts the process of in vitro rapid propagation of polyploid plants of red ginseng, synchronously multiplies and takes root, simplifies the tissue culture process, and carries out the whole culture process in one culture medium at the same time, greatly improving the artificial in vitro propagation efficiency. In addition, the present invention establishes a red fruit ginseng autopolyploid induction system; in the case of delayed polyploid growth, the direct organogenesis method is adopted to proliferate, while maintaining a high reproduction coefficient, greatly shortening the culture period, and reducing the cost. Low cost, short cycle time, high quality and high survival rate.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for polyploid induction of red fruit ginseng and an efficient artificial seedling cultivation method for polyploid plants. Background technique [0002] Red fruit ginseng (Campanumoea lancifolia (Roxb.) Merr.), is a perennial plant of Campanulaceae (Campanulaceae) genus Campanumoea (Campanumoea). ), mainly distributed in some countries in Southeast Asia, such as the Philippines, Vietnam, etc., in my country, it is only produced in some areas south of the Yangtze River, such as Yunnan and Sichuan, and grows in forests, shrubs and grasslands below 1500m above sea level. The root of red ginseng is used as medicine. It tastes sweet, slightly bitter, and flat in nature. It has the effects of tonifying deficiency and Qi, dispelling blood stasis and stopping bleeding. Another study shows that the leaves of red ginseng are rich in flavonoids, which are a class of natural...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 黄衡宇董鲜孟庆红李宏哲梁永丽
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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