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Method for polyploidy induction and efficient artificial seedling culture of polyploidy plants of campanumoea lancifolia

A technology of polyploidy and red fruit ginseng, which is applied in the biological field, can solve the problems of unrealized direct organ plants and other excellent traits, and achieve the effects of shortening the cultivation utilization period, increasing utilization value, and strong vitality

Active Publication Date: 2021-10-22
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is only one report on tissue culture of red fruit ginseng, which uses the method of explant-callus-adventitious buds, but does not realize direct organogenesis to ensure the transmission of excellent plant traits to the greatest extent.

Method used

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  • Method for polyploidy induction and efficient artificial seedling culture of polyploidy plants of campanumoea lancifolia
  • Method for polyploidy induction and efficient artificial seedling culture of polyploidy plants of campanumoea lancifolia
  • Method for polyploidy induction and efficient artificial seedling culture of polyploidy plants of campanumoea lancifolia

Examples

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Effect test

Embodiment 1

[0067] A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants, comprising the following steps:

[0068] 1. Acquisition of explants: Select robust plants with good growth potential and no pests and diseases, and choose the full fruits of the year.

[0069] 2. Wash the surface of the fruit in step 1 with tap water, soak it with 10% mass percentage washing powder solution for 10 minutes, shake it slightly, rinse it with running water for 30 minutes, place it on an ultra-clean workbench and treat it with 75% volume percentage ethanol solution for 30 seconds, and then Sterilize with 0.1% mercuric chloride aqueous solution by mass percentage for 30 minutes, and finally rinse with sterile water for 3 times, each time not less than 3 minutes, fully shake the vessel during the whole disinfection process.

[0070] 3. Acquisition of sterile seedlings: Cut open the fruit sterilized in step 2, take the inner seeds as material...

Embodiment 2

[0088] A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants, comprising the following steps:

[0089] 1. Acquisition of explants: Select robust plants with good growth potential and no pests and diseases, and choose the full fruits of the year.

[0090] 2. Wash the surface of the fruit in step 1 with tap water, soak it with 10% mass percentage washing powder solution for 10 minutes, shake it slightly, rinse it with running water for 30 minutes, place it on an ultra-clean workbench and treat it with 75% volume percentage ethanol solution for 30 seconds, and then Sterilize with 0.1% mercuric chloride aqueous solution by mass percentage for 30 minutes, and finally rinse with sterile water for 3 times, each time not less than 3 minutes, fully shake the vessel during the whole disinfection process.

[0091] 3. Acquisition of sterile seedlings: Cut open the fruit sterilized in step 2, take the inner seeds as material...

Embodiment 3

[0110] A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants, comprising the following steps:

[0111] 1. Acquisition of explants: Select robust plants with good growth potential and no pests and diseases, and choose the full fruits of the year.

[0112] 2. Wash the surface of the fruit in step 1 with tap water, soak it with 10% mass percentage washing powder solution for 10 minutes, shake it slightly, rinse it with running water for 30 minutes, place it on an ultra-clean workbench and treat it with 75% volume percentage ethanol solution for 30 seconds, and then Sterilize with 0.1% mercuric chloride aqueous solution by mass percentage for 30 minutes, and finally rinse with sterile water for 3 times, each time not less than 3 minutes, fully shake the vessel during the whole disinfection process.

[0113] 3. Acquisition of sterile seedlings: Cut open the fruit sterilized in step 2, take the inner seeds as material...

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Abstract

The invention provides a method for polyploidy induction and efficient artificial seedling culture of polyploidy plants of campanumoea lancifolia, which comprises the following steps: (1) inoculating disinfected and sterilized seeds into a culture medium A for sterile germination; (2) soaking stem tips with leaves of sterile seedlings in a sterilized colchicine solution, then transferring the stem tips into a culture medium B for culture, and then transferring the stem tips into a culture medium C; and (3) completing proliferative rooting of polyploidy plants in the culture medium C in one step. According to the method, the in-vitro rapid propagation process of the campanumoea lancifolia polyploidy plant is optimized and adjusted, proliferation and rooting are synchronously carried out, the tissue culture process is simplified, the whole culture process is carried out in one culture medium at the same time, and the artificial in-vitro propagation efficiency is greatly improved. In addition, a campanumoea lancifolia homologous polyploidy induction system is established; under the condition of delayed polyploidy growth, a direct organ generation mode is adopted for proliferation, meanwhile, a high propagation coefficient is kept, the culture period is greatly shortened, the cost is low, the period is short, and the quality and the survival rate are high.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for polyploid induction of red fruit ginseng and an efficient artificial seedling cultivation method for polyploid plants. Background technique [0002] Red fruit ginseng (Campanumoea lancifolia (Roxb.) Merr.), is a perennial plant of Campanulaceae (Campanulaceae) genus Campanumoea (Campanumoea). ), mainly distributed in some countries in Southeast Asia, such as the Philippines, Vietnam, etc., in my country, it is only produced in some areas south of the Yangtze River, such as Yunnan and Sichuan, and grows in forests, shrubs and grasslands below 1500m above sea level. The root of red ginseng is used as medicine. It tastes sweet, slightly bitter, and flat in nature. It has the effects of tonifying deficiency and Qi, dispelling blood stasis and stopping bleeding. Another study shows that the leaves of red ginseng are rich in flavonoids, which are a class of natural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 黄衡宇董鲜孟庆红李宏哲梁永丽
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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