Davallia mariesii breeding method

A high goat tooth and formula technology, which is applied in the fields of flower cultivation and fern reproduction, can solve the problems of difficult large-scale production, large dosage and high material requirements, and achieves the advantages of strong adaptability, stable seedling properties and improved economic benefits. Effect

Inactive Publication Date: 2013-05-15
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the defects and deficiencies such as high material requirements, large dosage, and difficulty in large-scale production in the

Method used

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  • Davallia mariesii breeding method
  • Davallia mariesii breeding method
  • Davallia mariesii breeding method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0047] Example 1

[0048] (1) Take the mature goat's tooth spores, wrap them in gauze, soak them in 75% alcohol for 30s in a super-clean environment, and then put them in a 0.1% mercury chloride solution for disinfection. Sterilize in the sodium hypochlorite mixture for 10 minutes, and then rinse with sterile water for 4 to 5 times for 2 minutes each time to obtain sterile spores of goat teeth. The sodium hypochlorite mixture is a mixture of sodium hypochlorite solution and Tween 20 , The ratio of sodium hypochlorite solution to Tween 20 is: add 2 drops of Tween 20 to 100 ml of 2% sodium hypochlorite solution.

[0049] (2) Induction and proliferation culture: Put the sterile spores of goat teeth obtained in step (1) into a culture flask containing the following proliferation induction medium:

[0050] MS basic medium

[0051]

[0052] Incubate in the dark for 15 days at a temperature of 23°C, switch to a light intensity of 2000lx, a temperature of 23°C, and a light time of 8h / d, cont...

Example Embodiment

[0060] Example 2

[0061] The other measures of Embodiment 2 are the same as those of Embodiment 1 except for the following measures, which will not be repeated. (1) Take the goat's tooth spores at the mature stage and wrap them with gauze, and soak them with 75% alcohol in a super-clean environment for 35s.

[0062] (2) The induction and proliferation culture medium is:

[0063] MS basic medium

[0064]

[0065] Incubate in the dark for 16 days at a temperature of 25°C, switch to a light intensity of 1800lx, a temperature of 25°C, and a light time of 9h / d. Continue to culture until the tooth spores of goats germinate and differentiate into green spheroids. Cut open.

[0066] (3) Subculture: 28 days for each subculture, and 3 times for subculture. The conditions of subculture are: light intensity is 1800lx, temperature is 25℃, light time is 9h / d, and subculture is 27d , The medium for subculture is the same as the inducing medium formula described in step (2).

[0067] (4) The rooting...

Example Embodiment

[0072] Example 3

[0073] The other measures of Embodiment 3 are the same as those of Embodiment 1 except for the following measures, which will not be repeated.

[0074] (1) Take the goat's tooth spores at the mature stage and wrap them with gauze, and soak them in 75% alcohol with a mass fraction of 40s in a super-clean environment.

[0075] (2) The medium for inducing proliferation is:

[0076] MS basic medium

[0077]

[0078] Incubate in the dark for 15 days at a temperature of 27°C, switch to a light intensity of 1500lx, a temperature of 27°C, and a light time of 10h / d, continue to cultivate until the tooth spores of goats germinate and differentiate into green spheroids, and the green spheroids Cut open.

[0079] (3) Subculture: each subculture 30 days, a total of 5 subcultures, the subculture conditions are: light intensity is 1500lx, temperature is 27℃, light time is 10h / d, subculture is 30d, The medium for subculture is the same as the inducing medium formula described in ste...

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Abstract

The invention discloses a Davallia mariesii breeding method, which comprises the following steps: an induction proliferation medium and a rooting medium are provided, the inducting and proliferation cultivating are concurrently carried out, after exercising seedling, and the seedling can be directly transplanted to a seedlings bag for culturing. The invention provides a technical scheme of a key link in a seedling breeding technology process of the Davallia mariesii, the proliferation rate is fast, the reproduction rates can reach 5-7 times during one period with 25-30 days; the rooting culture lasts about 20 days, each plant can breed 5-8 roots, the seedling is robust, the root develops, a tissue culture process is simplified, the cost and production time are greatly reduced, and the Davallia mariesii breeding method is suitable for standardization and industrial-scale production.

Description

technical field [0001] The invention belongs to the technical field of flower cultivation, in particular to the technical field of fern propagation. Background technique [0002] Alpine fern (Davallia mariesii) is a fern of the genus Drynariae in the Drynariaceae family. Originally produced in Guatemala, it entered China as cut leaves in the 1990s. It is a kind of fern suitable for cutting leaves. It is currently widely used in flower arrangement. Its various leaf shapes, leaf postures and green colors are pleasing to the eye. With the development of indoor ornamental plants, the supply of their products has been in short supply. Alpine fern propagation methods are mainly branch propagation and spore propagation, but the number of branch propagation is small, the plant type is easily damaged, the recovery is slow and limited by the season, and a large number of seedlings cannot be obtained in a short period of time; spore propagation is due to the spores in the natural env...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 许凤李绅崇杨春梅曹桦汪国鲜李金泽单芹丽阮继伟
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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