Novel method for callus induction and efficient plant regeneration of pineapple variant

A callus induction and high-efficiency regeneration technology, applied in the biological field, achieves the effects of short domestication and transplanting period, robust growth, and improved economic benefits

Pending Publication Date: 2021-09-07
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been many reports on pineapple tissue culture, and various automated liquid culture systems for micropropagation have also been tried, the expansion of tissue culture still needs to improve the proliferation coefficient and domestication scheme

Method used

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  • Novel method for callus induction and efficient plant regeneration of pineapple variant
  • Novel method for callus induction and efficient plant regeneration of pineapple variant
  • Novel method for callus induction and efficient plant regeneration of pineapple variant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] A new method for callus induction and plant efficient regeneration of pineapple mutant lines, comprising the following steps:

[0071] (1) Acquisition of explants: In the spontaneous mutant population, select robust plants with good growth potential and no pests and diseases, and choose the sucking buds.

[0072] (2) Disinfect the sucking buds of step (1) after peeling off the 5.0cm leaf base: wash the surface soil with tap water and then peel off the leaves, and soak the leaf base in 10% washing powder solution by mass for 10 min Slightly oscillate and stir, rinse with running water for 30 minutes, place on an ultra-clean workbench, treat with 75% ethanol solution by volume for 15 seconds, then sterilize with 0.1% mercury liter aqueous solution for 14 minutes, and finally rinse with sterile water 3 times, each time without Shake the vessel fully during the entire disinfection process if it is less than 3 minutes.

[0073] (3) Acquisition of callus: Cut the sucker leaf...

Embodiment 2

[0090] A new method for callus induction and plant efficient regeneration of pineapple mutant lines, comprising the following steps:

[0091] (1) Acquisition of explants: In the spontaneous mutant population, select robust plants with good growth potential and no pests and diseases, and choose the sucking buds.

[0092] (2) the sucking bud of step (1) is the method for carrying out disinfection treatment after peeling off 6.0cm leaf base: after cleaning with tap water and washing off surface soil, blade is peeled off, and its leaf base is soaked 10min with mass percentage 10% washing powder solution and Rinse with running water for 30 minutes after slight shaking and agitation, place on an ultra-clean workbench, treat with 75% ethanol solution by volume for 15 seconds, then sterilize with 0.1% mercury liter aqueous solution for 14 minutes, and finally rinse with sterile water 3 times, each time not lower Within 3 minutes, fully shake the vessel during the entire disinfection p...

Embodiment 3

[0110] A new method for callus induction and plant efficient regeneration of pineapple mutant lines, comprising the following steps:

[0111] (1) Acquisition of explants: In the spontaneous mutant population, select robust plants with good growth potential and no pests and diseases, and choose the sucking buds.

[0112] (2) the sucking bud of step (1) is the method that carries out disinfection after peeling off 7.0cm leaf base: after cleaning with tap water and washing off surface earth, blade is peeled off, and its leaf base is soaked 10min with mass percent 10% washing powder solution and Rinse with running water for 30 minutes after slight shaking and agitation, place on an ultra-clean workbench, treat with 75% ethanol solution by volume for 15 seconds, then sterilize with 0.1% mercury liter aqueous solution for 14 minutes, and finally rinse with sterile water 3 times, each time not lower Within 3 minutes, fully shake the vessel during the entire disinfection process.

[...

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Abstract

The invention discloses a novel method for callus induction and efficient plant regeneration of a pineapple variant. The novel method comprises the following steps: (1) obtaining an absorptive bud explant; (2) flushing and disinfecting absorptive buds in step (1), and inoculating the absorptive buds into a culture medium A to obtain calluses; (3) transferring the calluses generated from a root of a leaf base in step (2) into a culture medium B to perform callus proliferation and adventitious bud generation culture; (4) inoculating single seedlings with thick and strong basal stems in adventitious clumpy buds in step (3) into a culture medium C for rooting culture; and (5) seedling hardening and transplanting. In-vitro rapid propagation of the pineapple variant is optimized and adjusted, callus induction and proliferation and adventitious bud generation and proliferation are synchronously carried out, the tissue culture process is simplified, four culture processes are simultaneously carried out in one culture medium, the propagation efficiency is greatly improved, the cost is low, the time is short, the problem of low survival rate of seedling hardening and domestication of the pineapple test-tube plantlet is solved, and the seedling raising period is greatly shortened.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new method for callus induction and plant efficient regeneration of pineapple mutant lines. Background technique [0002] Pineapple (Ananascomsus L., Merr) is a perennial herbaceous plant belonging to the genus Ananas of the family Bromeliaceae, commonly known as pandanus and pineapple, and is one of the four famous fruits in the tropics and subtropics. There are 60 genera and more than 2,000 species of bromeliad plants; there are 5 species in the genus Bromelia: bud pineapple (A.bracteatus), rich pineapple (A.fritzmuelleri), edible pineapple (A.comosus), standing leaf pineapple ( A.erectifolius), Sude pineapple (A.ananassoiles), only edible pineapple has economic cultivation value. Pineapple is suitable for large-scale cultivation in tropical and subtropical regions, so it is grown in at least 26 countries, with China, India, Brazil, Thailand and the Philippines being...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 黄衡宇董鲜孟庆红徐福荣
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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