A method for artificial rapid propagation of orchid orchids by using embryogenic callus

Abstract

The invention discloses a method for artificial rapid propagation of orchid orchids by using embryogenic callus, which comprises the following steps: cutting open the sterilized and sterilized capsules, inoculating the seeds in the germination medium, and waiting for healing After the wound tissue appeared, embryo callus induction and proliferation, protocorm differentiation, growth and proliferation, rooting culture, seedling hardening and transplanting were carried out. The core of the present invention is that the embryogenic callus is produced after the seed coat of Orchid orchid is broken, and then differentiated into protocorms; on this basis, the culture medium is optimized and adjusted, and the embryogenic callus is proliferated, the protocorm is differentiated, Germination growth and proliferation are carried out simultaneously, which simplifies the tissue culture process, and the three culture processes are carried out in one medium at the same time, which greatly improves the reproduction efficiency. In addition, the invention has low cost, short period, high seedling quality and survival rate.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for artificial rapid propagation of orchid orchids by using embryogenic callus. Background technique [0002] Rhynchostylis is a perennial epiphytic herb of Orchidaceae (Orchidaceae), with about 6 species, mainly distributed in tropical Asia. There are 2 species in my country, namely Hainan rhynchostylis [R. gigantea (Lindl.) Ridl.] And diamond beak orchid [R.retusa (L.) Bl.], mainly produced in the southern tropical regions. Orchids are elegant and fragrant, with plump inflorescences, drooping or upright, resembling a furry fox tail. Therefore, their products are commonly known as "foxtail orchids", and they are a type of epiphytic orchids with high ornamental value. The flower color of Orchid orchid is relatively rich, including red, pink, white, blue, orange and other colors. Because of its unique flower shape, long flowering period and it is a traditional Chi...

AI Technical Summary

Problems solved by technology

[0003] The traditional propagation methods of orchid orchids are ramet propagation and seed propagation. Although ramet propagation can maintain the traits of the female parent, the efficiency is low, the multiplier is only 1-3 times, and the cycle is long, which is difficult to meet the needs of industrial production.

However, the seeds of most species of Orchidaceae are small and lack cotyledons and endosperms, resulting in incomplete embryo development. Under natural conditions, they need to cooperate with related symbiotic bacteria to germinate, which leads to long germination time and low germination rate (only 5%). , The seedling rate is low, and it generally takes 4-5 years or even longer from seed germination to flowering plants that can be characterized

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