A kind of artificial high-efficiency propagation method of Swertia japonica

Abstract

The invention discloses a method for artificial high-efficiency propagation of Swertia variegata. In the same medium, callus induction and basal shoot cluster formation can be carried out at the same time, and basal tube cluster bud formation and multiplication culture can be carried out simultaneously. The culture procedure is simplified to three steps; the entire rapid propagation process only needs three kinds of medium to solve the problems of callus, basal shoot clustering bud generation and proliferation, overcoming browning and vitrification, and rooting, saving inoculation procedures and The cultivation space is conducive to the arrangement of production plans; in addition, because the number of inoculations is reduced, the possibility of contamination is reduced, and the medium for callus induction and proliferation culture has the function of basal shoot clustering, which makes the biological material replace the medium It is easier to adapt, thereby improving the success rate of tissue culture of biological materials, and greatly improving the reproduction coefficient.

Description

Technical field: [0001] The invention relates to the propagation technology of Chinese herbal medicine, in particular to an artificial high-efficiency propagation method of Swertia japonica. Background technique: [0002] Swertia nervosa (G.Don) Wall. is a one-year or biennial herb of the genus Swertia in the Gentianaceae (Gentianaceae), also known as Hepatitis Grass, Four-edge Grass, Mountain Coptis, Herring Grass etc., mainly distributed in Southwest my country-Himalayan region, growing in river beaches, hillsides, sparse forests and bushes at an altitude of 460-2700m. Swertia japonica is a national medicine commonly used by the Tujia people in the southwest region. Its chemical components are mainly swertiamarin, gentiopicroside, oleanolic acid and 1,7-dihydroxy- 3,8-dimethoxyketone and other ketones have the functions of clearing heat and detoxification, clearing liver and promoting gallbladder, sedative and analgesic, etc. It has unique curative effect on the treatment ...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for artificial high-efficiency propagation of Swertia vegetativeis, in the same medium, the present invention can simultaneously induce callus and basal shoot clustering, and simultaneously carry out basal tuber clustering bud generation and proliferation Cultivation, simplifies the cultivation procedure to three steps; the entire rapid propagation process only needs three kinds of medium to solve the problems of callus, basal shoot clustering bud generation and proliferation, overcoming browning and vitrification, and rooting, saving inoculation The program and culture space are conducive to the arrangement of production plans; in addition, because the number of inoculations is reduced, the possibility of contamination is reduced, and the medium for callus induction and proliferation culture has the function of basal shoot clustering, which makes the replacement of biological materials The culture medium is easier to adapt, thereby improving the success rate of tissue culture of biological materials and greatly improving the reproduction coefficient

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