Strawberry
transplanting seedling tissue culture method, the steps include: A, selection of strawberry explants, B, disinfection and sterilization, C, anti-
browning culture, D,
callus induction, cluster
bud generation and proliferation culture, E, expansion Propagation and cultivation, F, rooting and cultivation, G,
seedling hardening and
transplanting. The beneficial effect is that the
tissue culture procedure of strawberry seedlings is simple, and
callus induction, cluster
bud formation and proliferation culture can be carried out simultaneously in the same medium; the
tissue culture period is short, the
reproduction coefficient is high, and the whole rapid propagation process only needs three kinds of culture The problem of
browning,
callus, cluster
bud generation and proliferation, and rooting can be solved based on the basis; adventitious root induction of test-tube seedlings does not add phytohormones, and the
survival rate of hardened seedlings is high and grows rapidly; seedlings can maintain the same
genotype, Progeny are not prone to
browning, and there is little
virus accumulation; the tissue culture method of inhibiting browning is low in cost, short in time, and the seedlings produced are of good quality,
high survival rate, and stable properties, which can meet the industrial production of high-quality strawberry seedlings need.