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32results about How to "Easy to factory operation" patented technology

Tissue culture method for strawberry transplant seedlings

Strawberry transplanting seedling tissue culture method, the steps include: A, selection of strawberry explants, B, disinfection and sterilization, C, anti-browning culture, D, callus induction, cluster bud generation and proliferation culture, E, expansion Propagation and cultivation, F, rooting and cultivation, G, seedling hardening and transplanting. The beneficial effect is that the tissue culture procedure of strawberry seedlings is simple, and callus induction, cluster bud formation and proliferation culture can be carried out simultaneously in the same medium; the tissue culture period is short, the reproduction coefficient is high, and the whole rapid propagation process only needs three kinds of culture The problem of browning, callus, cluster bud generation and proliferation, and rooting can be solved based on the basis; adventitious root induction of test-tube seedlings does not add phytohormones, and the survival rate of hardened seedlings is high and grows rapidly; seedlings can maintain the same genotype, Progeny are not prone to browning, and there is little virus accumulation; the tissue culture method of inhibiting browning is low in cost, short in time, and the seedlings produced are of good quality, high survival rate, and stable properties, which can meet the industrial production of high-quality strawberry seedlings need.
Owner:玉溪市祥馨农业技术开发有限公司

Fast asexual propagation method for maca hybrid

The invention discloses a fast asexual propagation method for maca hybrid. The method comprises the following steps: inoculating a sterilized maca hybrid explant into a callus inducing medium, generating callus, and the generated callus into a clump seedling generating and breeding medium to cultivate; when clump seedlings are generated and bred to a certain quantity, carrying out subculture multiplication in the breeding medium; when the quantity of the bred seedlings reaches the rooting requirements, transferring healthy main seedlings in the clump seedlings into a rooting medium to cultivate; and when the seedlings are healthy and the roots are thick, carrying out hardening-seedling for 3 days according to a conventional method, and transplanting into a floating plate with disinfection humus and cultivating for 60 days so as to obtain the healthy maca hybrid clonal seedlings. The production technology of the maca hybrid clonal seedlings is optimized and adjusted; after the callus and the clump seedlings are obtained, MS with high NH<4+> and B5 with low NH<4+> are adopted as basic culture mediums for alternative use in enrichment culture; the phenomena that a maca test-tube plantlet is easy to age and yellow are thoroughly solved; the asexual propagation cycle is shortened; the breeding efficiency is improved; and an effective way is provided for breeding of F1 generation of high-quality seedlings of the maca hybrid.
Owner:会泽枢康中草药种植有限公司

Artificial efficient propagation method of aristolochia tuberosa

The invention discloses an artificial efficient propagation method of aristolochia tuberosa. The method comprises the following steps of inoculating a disinfected and sterilized explant with a bud stem segments of the aristolochia tuberosa into a culture medium A for culture, starting propagation culture under the conditions that the illuminance is 1500-2000lx, the illumination time is 10h/d and the temperature is controlled to be 22 +/-1 DEG C, and obtaining a propagation coefficient of 3.57 after 35 days. A material is shorn into the stem segments containing 2-3 joints, the stem segments areinoculated into a culture medium B to be cultured, a clustered cluster system with base adventitious cluster buds and upper axillary buds growing is formed after 45 days, and the propagation coefficient reaches 10.0 or above. When the height of proliferated cluster buds is 3-4cm, single seedlings are inoculated into the culture medium B to be cultured for 45d to obtain rooted seedlings with strong seedlings and thick roots, and then the rooted seedlings are transplanted. The method is low in cost, short in period and high in test-tube plantlet quality and survival rate, provides technical support for protecting wild resources and breeding high-quality plantlets, and can be used for producing excellent plantlets with consistent genotype backgrounds in a short time.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Rapid reproduction method of schlumbergera seedlings

The invention provides a rapid reproduction method of schlumbergera seedlings. The method comprises the following steps: inoculating a selected, disinfected and sterilized schlumbergera explant onto a culture medium for induction and carrying out synchronous induction and proliferation culture until the explant sprouts and differentiates out a bud; sub-culturing until being proliferated to 3-4 folds, and then transferring to a rooting medium for rooting culture until 3-5 fine roots are grown at the base of a plant; conventionally hardening and then transplanting to a mixed substrate of humus soil, red soil and pearlite; and watering and fertilizing conventionally for growing to obtain the schlumbergera seedlings. In the method, key links in the whole production technical flow of the schlumbergera seedlings are optimized and integrated, the culture medium for induction is blended and bud induction and proliferation culture are carried out synchronously, thus simplifying tissue culture procedure and shortening breeding cycle; and only by adopting two types of the culture media, the bud can be directly formed without callus formation, thus reducing probability of variation occurred on an adventitious bud, effectively controlling generation of a variant strain, being capable of fixing and maintaining fine variety of the schlumbergera seedlings rapidly, improving seedling quality and solving the problems of complicated parental source and unstable quality of the seedlings produced by the conventional breeding system.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

New method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino

The invention discloses a new method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino.The method comprises the following steps: (1) obtaining an explant; (2) firstly washingsoil on the surface of pseudobulb in step (1) with tap water, then soaking with a washing powder solution, then rinsing with running water, then treating with an ethanol solution, and rinsing after disinfection; (3) obtaining sterile seedlings; (4) transferring young shoots grown from buds cultured in step (3) to a medium A in step (3) for enrichment culture, cutting off seedlings with callus, andcontinuing to transfer to a medium B for culture; (5) inoculating a medium C with single seedlings with rice grain-sized pseudobulb after subculture in step (4) for culture; (6) exercising the seedlings and transplanting. The method disclosed by the invention has the advantages that the in-vitro rapid propagation of the cremastra appendiculata (D.Don) Makino is optimized and adjusted; the processof artificial rapid propagation is simplified; the propagation efficiency is greatly improved; the cost is low and the time is short; not only is the survival rate of tube seedlings through seedlingexercising improved, but the nursery cycle is also greatly shortened.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Asexual Rapid Propagation Method of Maca Hybrid

The invention discloses a fast asexual propagation method for maca hybrid. The method comprises the following steps: inoculating a sterilized maca hybrid explant into a callus inducing medium, generating callus, and the generated callus into a clump seedling generating and breeding medium to cultivate; when clump seedlings are generated and bred to a certain quantity, carrying out subculture multiplication in the breeding medium; when the quantity of the bred seedlings reaches the rooting requirements, transferring healthy main seedlings in the clump seedlings into a rooting medium to cultivate; and when the seedlings are healthy and the roots are thick, carrying out hardening-seedling for 3 days according to a conventional method, and transplanting into a floating plate with disinfection humus and cultivating for 60 days so as to obtain the healthy maca hybrid clonal seedlings. The production technology of the maca hybrid clonal seedlings is optimized and adjusted; after the callus and the clump seedlings are obtained, MS with high NH<4+> and B5 with low NH<4+> are adopted as basic culture mediums for alternative use in enrichment culture; the phenomena that a maca test-tube plantlet is easy to age and yellow are thoroughly solved; the asexual propagation cycle is shortened; the breeding efficiency is improved; and an effective way is provided for breeding of F1 generation of high-quality seedlings of the maca hybrid.
Owner:会泽枢康中草药种植有限公司

Tissue cultivaition method of vetiveria zizanioides transplant

The invention provides a tissue cultivation method of vetiveria zizanioides transplants. The method comprises steps that: sterilizated vetiveria zizanioides explants are placed in induction and proliferation culture media; inducting and proliferation cultivating are carried out concurrently until explants are differentiated and young buds are germinated; times of subcultures are carried out untila required number of seedlings are obtained; the seedlings are transplanted into cultivation media to be cultivated until 3 to 5 roots appear; the seedlings are then transplanted into cultivation bags; routine managements of watering and fertilizing are carried out, and vetiveria zizanioides transplants can be obtained after 40 days of growth. With the method provided by the present invention, usage amounts of drugs can be reduced. Through the concurrent inducting and proliferation cultivating, appearances of variants can be efficiently controlled, tissue cultivating processes can be simplified, propagation periods can be shortened, production cost can be reduced, and merits of vetiveria zizanioides varieties can be kept fast and stably. With the method provided by the present invention, problems in methods with common propagation systems, such as complex parent resources, unstable seedling qualities and slow seedling propagations, can be solved.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

Rapid reproduction method of schlumbergera seedlings

The invention provides a rapid reproduction method of schlumbergera seedlings. The method comprises the following steps: inoculating a selected, disinfected and sterilized schlumbergera explant onto a culture medium for induction and carrying out synchronous induction and proliferation culture until the explant sprouts and differentiates out a bud; sub-culturing until being proliferated to 3-4 folds, and then transferring to a rooting medium for rooting culture until 3-5 fine roots are grown at the base of a plant; conventionally hardening and then transplanting to a mixed substrate of humus soil, red soil and pearlite; and watering and fertilizing conventionally for growing to obtain the schlumbergera seedlings. In the method, key links in the whole production technical flow of the schlumbergera seedlings are optimized and integrated, the culture medium for induction is blended and bud induction and proliferation culture are carried out synchronously, thus simplifying tissue culture procedure and shortening breeding cycle; and only by adopting two types of the culture media, the bud can be directly formed without callus formation, thus reducing probability of variation occurred on an adventitious bud, effectively controlling generation of a variant strain, being capable of fixing and maintaining fine variety of the schlumbergera seedlings rapidly, improving seedling quality and solving the problems of complicated parental source and unstable quality of the seedlings produced by the conventional breeding system.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

Artificial efficient propagation method of swertia nervosa (G.Don)Wall.

The invention discloses an artificial efficient propagation method of swertia nervosa (G.Don)Wall.. According to the method, in the same culture medium, callus induction and basal stem multiple shootgeneration can be conducted simultaneously, basal stem multiple shoot generation and multiplication culture can also be conducted simultaneously, and thus the culture procedure is simplified into three steps; in the whole rapid propagation process, only three kinds of culture mediums are needed to achieve the purposes of callus induction, basal stem multiple shoot generation, and multiplication culture, avoid the phenomena of browning and vitrification and solve the problem of root taking, the inoculation procedures and the culture space are saved, and arrangement of production plans is facilitated; besides, since the inoculation frequency is reduced, the possibility of contamination is lowered; the culture mediums for callus induction and multiplication culture have the function of basalstem multiple shoot generation, so that biological materials better adapt to replacement of the culture mediums, the success rate of tissue culture of the biological materials is increased, and the propagation coefficient is greatly increased.
Owner:JISHOU UNIVERSITY

The method of overcoming burning tip necrosis and improving the efficiency of artificial rapid propagation of Flaxa grandiflorum

The invention discloses a method for improving the efficiency of artificial rapid propagation of flaxgrass overcoming burnt tip necrosis, which belongs to the field of biotechnology, and comprises the following steps: inoculating a sterilized and sterilized explant of a segmented stem segment into a culture medium, Induce the germination of axillary buds, the occurrence and proliferation of clustered buds on the base stems, overcome the necrosis of burnt tips, rejuvenate and take root, and transplant hardened seedlings. The invention completely solves the phenomenon of burning tip necrosis common in in vitro rapid propagation of plants of the family Scrophulariaceae, especially of the genus Flax, and has low cost, short cycle, high seedling quality and survival rate. In addition, the production process of aseptic seedlings of C. grandiflorum was optimized and adjusted. While improving burn tip necrosis, axillary bud germination and growth, basal stem cluster bud induction and growth were simultaneously carried out, and the tissue culture process was simplified. Three culture processes At the same time in a culture medium, greatly improving the reproduction efficiency.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Artificial high-efficiency clonal propagation method of dendrobium anosmum

The invention provides an artificial high-efficiency clonal propagation method of dendrobium anosmum. The method comprises the following steps: inoculating a dendrobium anosmum stem explant with budssubjected to disinfection sterilization treatment into a culture medium A, and performing culturing under the conditions that the illuminance is 1500-2000lx and the illumination time is 10h/d; starting multiplication culture under the condition that the temperature is controlled to be 25 +/-1 DEG C, so that the base number of the material is increased, and the multiplication coefficient can be about 2.35 after 75 days; shearing the material into a stem segment containing 2-3 nodes, inoculating the stem segment into a culture medium B, and performing culturing for 60 days to form a multi-bud clustered system with a propagation coefficient of 6.0 or above; enabling adventitious roots to be synchronously induced in the culture medium, wherein the rooting rate reaches 100%; and the survival rate of rooted seedlings reaching 100% after the rooted seedlings are subjected to acclimatization for 60 days. The method is low in cost, short in period and high in test-tube plantlet quality and survival rate, provides technical support for protecting wild resources and breeding high-quality plantlets, and can produce excellent plantlets with consistent genotype backgrounds in a short time.
Owner:云南华农农业有限公司
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