Strawberry transplanting seedling tissue culture method, the steps include: A, selection of strawberry explants, B, disinfection and sterilization, C, anti-browning culture, D, callus induction, cluster bud generation and proliferation culture, E, expansion Propagation and cultivation, F, rooting and cultivation, G, seedling hardening and transplanting. The beneficial effect is that the tissue culture procedure of strawberry seedlings is simple, and callus induction, cluster bud formation and proliferation culture can be carried out simultaneously in the same medium; the tissue culture period is short, the reproduction coefficient is high, and the whole rapid propagation process only needs three kinds of culture The problem of browning, callus, cluster bud generation and proliferation, and rooting can be solved based on the basis; adventitious root induction of test-tube seedlings does not add phytohormones, and the survival rate of hardened seedlings is high and grows rapidly; seedlings can maintain the same genotype, Progeny are not prone to browning, and there is little virus accumulation; the tissue culture method of inhibiting browning is low in cost, short in time, and the seedlings produced are of good quality, high survival rate, and stable properties, which can meet the industrial production of high-quality strawberry seedlings need.