A kind of breeding method of pollen-free lily variety
A breeding method and pollen-free technology, applied in flower cultivation, horticultural methods, botanical equipment and methods, etc., can solve the problems of wasting manpower and material resources, easily polluting clothes, inconvenient cleaning, etc., and achieve improved survival rate, easy standardization, and source single effect
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Embodiment 1
[0085] A method for cultivating pollen-free lily varieties, comprising the steps of:
[0086] Step A: In July, when the lily is in full bloom, select the male oriental lily flower pellets, and artificially pollinate the female oriental lily in a greenhouse at no higher than 25°C, and wrap the stigma of the female oriental lily with tinfoil after pollination ;
[0087] Step B, pollination 85 days, select the lily fruit with enlarged capsule, observe every day, take off the capsule when the capsule is mature, but the back suture is not cracked, then wipe the capsule twice with alcohol with a mass concentration of 75%, and then remove the capsule first Put it into a mercuric chloride solution with a mass concentration of 0.1% for disinfection for 15 minutes, then put it into the first mixed solution for disinfection for 15 minutes, then rinse it with sterile water for 3 times, each time for 1 minute, to obtain lily seed explants;
[0088] The preparation method of the first mixe...
Embodiment 2
[0155] A method for cultivating pollen-free lily varieties, comprising the steps of:
[0156] Step A: In July, when the lily is in full bloom, select the male oriental lily flower pellets, and artificially pollinate the female oriental lily in a greenhouse at no higher than 25°C, and wrap the stigma of the female oriental lily with tinfoil after pollination ;
[0157] Step B, pollination 95 days, select the lily fruit with enlarged capsule, observe every day, take off the capsule when the capsule is mature, but the back suture is not cracked, then wipe the capsule twice with alcohol with a mass concentration of 75%, and then remove the capsule first. Put it into a mercuric chloride solution with a mass concentration of 0.1% for disinfection for 15 minutes, then put it into the first mixed solution for disinfection for 15 minutes, then rinse it with sterile water for 3 times, each time for 1 minute, to obtain lily seed explants;
[0158] The preparation method of the first mix...
Embodiment 3
[0225] A method for cultivating pollen-free lily varieties, comprising the steps of:
[0226] Step A: In July, when the lily is in full bloom, select the male oriental lily flower pellets, and artificially pollinate the female oriental lily in a greenhouse at no higher than 25°C, and wrap the stigma of the female oriental lily with tinfoil after pollination ;
[0227] Step B, pollinate 90 days, select the lily fruit with enlarged capsule, observe every day, take off the capsule when the capsule is mature, but the back suture is not cracked, then wipe the capsule twice with alcohol with a mass concentration of 75%, and then remove the capsule first Put it into a mercuric chloride solution with a mass concentration of 0.1% for disinfection for 15 minutes, then put it into the first mixed solution for disinfection for 15 minutes, then rinse it with sterile water for 3 times, each time for 1 minute, to obtain lily seed explants;
[0228] The preparation method of the first mixed ...
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