39results about How to "Solve the problem of rooting" patented technology

The invention belongs to the fields of forestry biotechnological seed breeding or plant cell engineering and discloses an E.urophylla*E.saligna rooting culture medium and an application thereof. On the basis of an improved MS culture medium, IBA and 3-AB are supplied to the rooting culture medium. By means of addition of the novel rooting inductive substance 3-AB and the synergistic effect of the rooting-type growth regulator IBA, rooting ratio of the E.urophylla*E.saligna is more than 87% and transplanting survival rate is more than 91%. When rooted seedlings are transplanted on bags, the seedlings grow tidily and robustly, have strong resistance, and completely reach demands of large-scale and commercial seedling production. The rooting culture medium has good repeatability and stable effects, and has no intensive fluctuation on the rooting ratio and transplanting survival rate even after multiple times of usage, thereby completely solving a problem that the E.urophylla*E.saligna is difficult to root during tissue culture induction.

The invention discloses a direct polygerm-generation regeneration plant culture method by the isolated culture of Xinjiang snow locus epicotyl. The invention also discloses a decapitated epicotyl exopThe invention discloses a direct polygerm-generation regeneration plant culture method by the isolated culture of Xinjiang snow locus epicotyl. The invention also discloses a decapitated epicotyl exophyte. The invention utilizes an epicotyl exophyte and the decapitated epicotyl exophyte to establish a gene-independent, simple and rapid Xinjiang snow locus isolated culture regeneration plant culturhyte. The invention utilizes an epicotyl exophyte and the decapitated epicotyl exophyte to establish a gene-independent, simple and rapid Xinjiang snow locus isolated culture regeneration plant culture technology which directly induces polygerm generation, elongation and rootage; by the formulation of a culture medium and the optimizing screen of carbon sources, the browning degree of the exophytee technology which directly induces polygerm generation, elongation and rootage; by the formulation of a culture medium and the optimizing screen of carbon sources, the browning degree of the exophyteis lowered to the lightest degree, an exophyte can have the maximum generation bud number more than or equal to 3 and the regeneration bud rootage rate higher than 90 percent, and a regeneration plan is lowered to the lightest degree, an exophyte can have the maximum generation bud number more than or equal to 3 and the regeneration bud rootage rate higher than 90 percent, and a regeneration plant with a full root, a full stalk and full leaves can be obtained by only 3 to 4 months. The invention has low malformed seedling rate and lays a foundation for the hereditary improvement of Xinjiang st with a full root, a full stalk and full leaves can be obtained by only 3 to 4 months. The invention has low malformed seedling rate and lays a foundation for the hereditary improvement of Xinjiang snow locus breeds applied in production during gene engineering.now locus breeds applied in production during gene engineering.

The soil-less high stem planting process for Rosa canina includes the following steps: selecting cutting wood of 70-150 cm length from annual branch in Feb or March, inserting the lower end of the cutting wood into ABT1 rooting powder solution for 10-30 min, inserting in culture medium comprising perlite, vermiculite and turfy soil in the weight ratio of 2 to 1 to 3 inside greenhouse, maintaining the temperature in 15-30deg.c and air humidity over 80 %, applying 800-1000 times diluted badistan solution once each week to root, transplanting to land, water and fertilizer management, grafting with Chinese rose bud in Aug to Oct, cutting stock in the next spring, and transplanting in the end of the next year. The process has easy operation, high efficiency, and short production period.

The invention discloses an epicotyl explant (epicotyl explant) of a cotton in vitro culture and direct multi-bud generation regenerated plant and a cultivation method for obtaining a complete regenerated plant by using the explant. The invention also discloses a decapitated epicotyl explant. The present invention utilizes epicotyl explants and fresh epicotyl explants to establish a technique for directly inducing multi-bud generation, elongation and rooting that does not depend on genotype, simple and fast cotton in vitro culture regeneration plant technology, Through the optimal screening of medium formula and carbon source, the degree of browning of explants is reduced to the lightest, the maximum number of buds in one explant is ≥ 3, and the rooting rate of regenerated shoots is above 90%, obtaining a plant with complete roots, stems and leaves. It only takes 3 to 4 months to regenerate plants, and the rate of deformed seedlings is low, which lays the foundation for the genetic improvement of cotton varieties used in production.

The invention relates to the technical field of plant propagation, in particular to a method for asexual propagation of Samara oil tree, which solves the problem of low seedling germination rate and emergence rate in the existing propagation method of Samara oil tree, and it is difficult to produce new shoots when cutting twigs. Root and other problems, including building seedbeds; collecting cuttings on the mother tree; cutting the collected cuttings in the seedbed matrix; spraying water on the leaves of Samara oil tree after cuttings, the temperature is 20-30 ° C; fertilizing the leaves , until the spikes take root. The invention utilizes asexual reproduction technology to reproduce more seedlings in a short period of time, adjust the temperature and humidity of the seedbed, maintain the physiological balance of the cuttings, and carry out disinfection and plant growth hormone treatment on the cut cuttings to provide the required growth for the cuttings to take root. At the same time, the mother tree is technically treated to promote the formation and development of adventitious roots of cuttings, effectively solving the problem of difficult callus rooting. The propagation technology is simple and fast, with low cost and high reproduction rate.

The invention belongs to the field of forestry biotechnology breeding and discloses a eucalyptus urophylla rooting medium and application. According to the rooting medium, root induction substances, such as IBA, ABT-1, 3-AB and 3-AB, are added on the basis of an improved MS culture medium formula so as to serve as the conventional cytostatics, and by matching with the other two root induction substances, 3-AB achieves an obvious effect of inducing adventitious roots, so that the growth and development directions of cambial cells are changed, a parenchymal cell state is developed into a root primordium form of the adventitious roots, and the cells are decreased and increased, continuously divided along the periclinal direction and finally break through the epidermis to grow into lateral roots. According to use of the rooting medium disclosed by the invention, the rooting rate of eucalyptus urophylla can reach over 85%. The survival rate can be over 90% after transplanting. Moreover, the rooting medium is high in reproducibility and stable in effect, and after repeated use of the medium, the rooting rate and transplanting survival rate do not have great fluctuation.

The purpose of the present invention is to disclose an in-bottle rooting method for a salt tolerance ulmus pumila tissue culture seedling. The method comprises the following steps: (1) selecting a secondary seedling, wherein a secondary culture salt tolerance ulmus pumila tissue culture seedling is selected, the height of the seedling is 2-3.5 cm, and the robust individual ulmus pumila seedling having extending leaves is selected; and (2) inoculating the selected secondary culture salt tolerance ulmus pumila tissue culture seedling onto a WPM rooting medium, wherein the WPM rooting medium is added with growth hormone, cytokinin and exogenous additives. According to the present invention, a medium formula having a high rooting rate is adopted so as to effectively solve the rooting problem of the salt tolerance ulmus pumila tissue culture seedling, improve the rooting rate of the salt tolerance ulmus pumila tissue culture seedling, reduce the cost, accelerate the factory production of the salt tolerance ulmus pumila, provide high quality salt tolerance ulmus pumila seedlings for nursery stock markets, and achieve the purpose of the present invention, wherein the rooting rate of the salt tolerance ulmus pumila tissue culture seedling can be up to 95%.

The present invention provides a method for rapidly growing seedlings by tissue culture of mature mature embryos of Chinese chestnut, comprising the following steps: (1) selecting, cleaning and disinfecting Chinese chestnuts of high quality; (2) obtaining and inoculating mature Chinese chestnut embryos; (3) Cultivation of explants; (4) domestication and hardening of tissue culture seedlings. The method provided by the invention uses mature embryos as explants to carry out tissue culture, which greatly improves the germination and seedling rate of Chinese chestnut, shortens the breeding cycle, accelerates the breeding of improved varieties, improves the efficiency of Chinese chestnut germplasm innovation and new variety selection, and promotes The industrial development of the geographical indication product "Luotian Chestnut" of the General Administration of Quality Supervision, Inspection and Quarantine is of great significance.

The invention discloses a direct polygerm-generation regeneration plant culture method by the isolated culture of beet epicotyl. The invention also discloses a decapitated epicotyl exophyte. The invenThe invention discloses a direct polygerm-generation regeneration plant culture method by the isolated culture of beet epicotyl. The invention also discloses a decapitated epicotyl exophyte. The invention utilizes an epicotyl exophyte and the decapitated epicotyl exophyte to establish a gene-independent, simple and rapid beet isolated culture regeneration plant culture technology which directly intion utilizes an epicotyl exophyte and the decapitated epicotyl exophyte to establish a gene-independent, simple and rapid beet isolated culture regeneration plant culture technology which directly induces polygerm generation, elongation and rootage; by the formulation of a culture medium and the optimizing screen of carbon sources, the browning degree of the exophyte is lowered to the lightest deduces polygerm generation, elongation and rootage; by the formulation of a culture medium and the optimizing screen of carbon sources, the browning degree of the exophyte is lowered to the lightest degree, an exophyte can have the maximum generation bud number more than or equal to 3 and the regeneration bud rootage rate higher than 90 percent, and a regeneration plant with a full root, a full stagree, an exophyte can have the maximum generation bud number more than or equal to 3 and the regeneration bud rootage rate higher than 90 percent, and a regeneration plant with a full root, a full stalk and full leaves can be obtained by only 3 to 4 months. The invention has low malformed seedling rate and lays a foundation for the hereditary improvement of beet breeds applied in production duringlk and full leaves can be obtained by only 3 to 4 months. The invention has low malformed seedling rate and lays a foundation for the hereditary improvement of beet breeds applied in production during gene engineering.gene engineering.

The invention discloses an ecological slope protection plant measure method for high and steep slopes of concrete and rocks. Concrete water storage tanks and self-flowing micro-irrigation systems are used on slopes such as concrete and rocks to solve the problem that water on steep slopes cannot enter the soil by gravity. At the same time, reinforced planting holes, water holes and ventilation holes are used on slopes to solve The water, nutrients and air permeability necessary for plant growth. After the roots of the plants are developed, a plant-reinforced wall is formed while the slope is greening, which is beneficial to the improvement of shallow landslides and achieves the purpose of ecological slope protection. Through the measures and methods of the present invention, ecological control is carried out on the parts where the roots of plants on high and steep slopes such as shotcrete, concrete, and rocks are difficult to grow, and the plant survival rate is high, the planting range is wide, the survival time is long, the landscape effect is good, and the environmental impact is small. Strong wind resistance, water erosion resistance, and freezing erosion resistance, low investment, less material consumption, low labor intensity for workers, and short construction period.

The invention belongs to the technical field of plant tissue culture, and in particular relates to a rooting method in a bottle for tissue cultured seedlings of Xianmaixiang tea. The method for rooting in a bottle of the tissue-cultured plantlets of Xianmaixiang tea vegetables of the present invention, the steps are as follows: (1) Select the tissue culture seedlings of Xianmaixiang tea vegetables subcultured, the selection standard is a height of 3-4cm, normal growth and no glass (2) inoculate the selected subcultured Xianmaixiang tea seedlings on 1 / 4MS rooting medium; add growth hormone to the 1 / 4MS medium. The present invention determines the optimal rooting medium through a large number of experiments, can effectively solve the rooting problem of Xianmaixiang tea vegetable, and significantly improves the rooting rate of rooting in the tissue culture bottle of Xianmaixiang tea vegetable, and the rooting rate is as high as 95%. The above, while reducing the production cost, can provide the market with a large number of high-quality tissue culture seedlings to meet the growing demand of the market, and then effectively promote the industrialized production process.