39results about How to "Solve the problem of rooting" patented technology

The invention relates to a plant breeding field, which in detail relates to a method of elaeagnus mollis asexual propagation, comprising following steps: building seed bed; collecting ear from parent tree; cutting collected ear in medium on the bed; spraying water on leaves of elaeagnus mollis, the water temperature is 20-30 Deg. C; fertilizing on leaves until ear grows out root. The invention employs technology of asexual propagation to breed more seedling in short time, moderates seedling bed's temperature and humidity to keep physiological balance for cottage ear, disinfects ear and treats it with plant growth hormone to provide bios and nutrition for ear rooting, and treats parent tree to promote formation and growth of adventitious root. It solves problems of difficult rooting of callus and low seedling rate. The invention is characterized by simple and fast propagation technique, low cost and high propagation rate.

The invention relates to a method for tender branch cutting propagation of a Linze jujube, comprising the following steps of: selecting tender branches, wherein the selected tender branches are the tender branches grown from jujube saplings with semi-lignification under high temperature; collecting the tender branches, soaking tender branch cuttings in a well-prepared mixed solution; constructing a seedbed through the steps of sanding, disinfecting, heat preserving, light and moisture regulating and sterilization; and then carrying out cutting propagation and acclimatization. The invention is a duplicating jujube plantlet method by using current-grown tender branches as propagation materials. The duplication process is actually a root getting process, by which rootless branches are grown into rooting plants after being soaked in auxin and an auxin synergist solution. Compared with the most advanced traditional tissue-cultured seedling method, the invention has breakthroughs on two aspects of: 1, capability of directly taking self-rooted seedlings, which solves the problem of hard root-taking of jujube seedlings; and 2, propagation of pure and high-quality plantlets, which solves the bottleneck problem of propagating the pure and high-quality seedlings in the appropriate technology field through the ages.

The invention discloses a coptis chinensis planting method; the planting method is characterized by comprising the steps: (1) preparation of a planting land; (2) furrowing; (3) seed treatment; (4) planting; (5) cultivation and (6) pest control. The problems of being difficult in rooting and low in reproductive rate are solved effectively by choosing the comprehensive measures such as proper matrixes, proper soaking agent, planting manner and proper temperature and humidity; and the reproduction speed is improved effectively. The coptis chinensis planting method is low in cost; with adoption of an atomizing water and fertilizer management and temperature/humidity control system, the method is simple and practical and is an effective method for large-scale planting of coptis chinensis.

The soil-less high stem planting process for Rosa canina includes the following steps: selecting cutting wood of 70-150 cm length from annual branch in Feb or March, inserting the lower end of the cutting wood into ABT1 rooting powder solution for 10-30 min, inserting in culture medium comprising perlite, vermiculite and turfy soil in the weight ratio of 2 to 1 to 3 inside greenhouse, maintaining the temperature in 15-30deg.c and air humidity over 80 %, applying 800-1000 times diluted badistan solution once each week to root, transplanting to land, water and fertilizer management, grafting with Chinese rose bud in Aug to Oct, cutting stock in the next spring, and transplanting in the end of the next year. The process has easy operation, high efficiency, and short production period.

The invention discloses a method of utilizing cornus walteri superior-tree twigs for cuttage breeding. The method includes that middle-upper twigs of current-year semi-lignified branches of a cornus walteri superior-tree are utilized as cuttings, three hormone solutions different in mass concentration and a disinfectant are used to treat the cuttings, cuttage facilities are set up, a matrix is prepared, and cuttage and management after cuttage are performed to induce rooting of cornus walteris cuttings and increase cutting rooting rate. The rooting rate reaches 92.7%, the technical problem oflow rooting rate caused by the fact that cornus walteris twigs are utilized for cuttage breeding is solved effectively, and the objective of rapid propagation of cornus walteris high-quality germplasmis achieved. By applying the method, a lot of high-quality cuttage seedlings of cornus walteris can be provided in short time, large-scale seedling culture standards are reached, and domestic demandon high-quality seedlings of cornus walteris can be met.

The invention discloses an ecological slope protection plant measure method for concrete and rock high steep slope surfaces. A concrete water storage tank and a self-flow type micro-irrigation systemare arranged on the concrete and rock slope surfaces to solve the problem that moisture in the steep slope surfaces cannot enter the soil depending on gravity, and reinforced plant planting holes, moisture holes and ventilated topdressing holes are adopted in the slope surfaces to solve the necessary moisture, nutrients and breathability for plant growth. After root hairs of plants are developed,side slopes are greened while a plant reinforcement wall is formed, the improvement of shallow landslides is facilitated, and the purpose of ecological slope protection is achieved. According to the ecological slope protection plant measure method for the concrete and rock high steep slope surfaces, ecological management is carried out on parts such as sprayed concrete, concrete and rocks where the root hairs of the plants are difficult to grow, the rate of survival of the plants is high, planting ranges of the plants are wide, the survival time is long, the landscape effect is good, the environmental influence is small, the wind resistance, water flow corrosion resistance and freezing corrosion resistance capacities are high, the investment is saved, the material expense is less, the labor intensity of workers is low, and the construction period is short.

The invention provides a method for strengthening and rooting tissue culture seedlings of transgenic peanuts. The method has a good seedling strengthening effect and is high in rooting rate and rooting quality. The method comprises the following steps of: (1) transferring peanut tissue culture seedlings on which cluster buds are induced and differentiated into a seedling strengthening culture medium A, culturing for 20 days, and replacing the culture medium once every 10 days, wherein a component of the seedling strengthening culture medium A is a Murashige and Skoog (MS) culture medium, and used hormone is cytokinin 6-benzylaminopurine (6-BA); (2) dividing the tissue culture seedlings with the heights of more than 1.5cm into single plants, transferring into a seedling strengthening culture medium B, culturing for 20 to 25 days, and replacing the culture medium once every 10 days, wherein a component of the seedling strengthening culture medium B is the MS culture medium, and used hormone is cytokinin 6-BA at the concentration of 0.4mg/L and auxin naphthyl acetic acid (NAA) at the concentration of 0.1mg/L; and (3) transferring into a rooting culture medium when the seedlings with the heights of 2.5 to 4cm, and rooting, wherein a component of the rooting culture medium is a one/second MS culture medium, and used hormone is auxin indolebutyric acid (IBA) at the concentration of 3mg/L and auxin NAA at the concentration of 0.3mg/L.

The invention provides a method for culturing rapid propagation seedlings in superior-species chestnut mature embryo tissue. The method comprises the following steps of 1, selecting, cleaning and disinfecting superior-species chestnuts; 2, obtaining and inoculating chestnut mature embryos; 3, culturing an explant; 4, performing acclimation seedling hardening of tissue culture seedlings. Accordingto the method, mature embryos are adopted as explants for performing tissue culture, the germination planting percent of chestnuts is greatly increased, the breeding period is shortened, stock breeding is accelerated, the chestnut germ plasm innovation and new-variety breeding efficiency are improved, and the method is of a great significance in pushing the industrial development of the State Quality Inspection Administration geographic marking product 'Luotian chestnut'.

The invention discloses a populus hopeiensis seedling growing method for combining bud grafting and cuttage. Grafting is selected to be carried out from June 20 to July 20; from March 20, populus hopeiensis bud cion seedlings are collected from a stock garden, and then cutting slips are made through shearing; cutting slips are stored at high-terrain shady spots with good drainage performance, burying sand needs to be disinfected with potassium permanganate before burying, and the humidity and temperature of the burying sand are kept after storage; on about April 20, cuttage is carried out when soil is just unfrozen. According to the populus hopeiensis fast grafting seedling growing method, the propagation coefficient of populus hopeiensis hard to propagate can be increased, the problem about hard rooting of the populus hopeiensis is solved, the seedling growth quality is improved, resources are saved, and the method has important significance on land greening and ecological management of mountains, water, forests, land and lakes.

A technique for directly polygemmic regenerative plant of cotton by in vitro culture of its epicotyl explant and a decapitated epicotyl explant are disclosed.

The invention provides a rocky hill afforestation method, comprising the steps of digging tree holes in a rocky barren hill land, filling the tree holes with soil and water required by plantlets to grow, transplanting plantlets to the tree holes, and managing the transplanted plantlets. By using the rocky barren hill afforestation method, survival rate of the transplanted plantlets can be effectively increased and may be increased to 95%.

The invention discloses a rapid propagation method for excellent Chinese fir family seedlings, and belongs to the technical field of Chinese fir cultivation. The rapid propagation method comprises the following steps: (1) excellent family grafting, (2) explant disinfection, (3) induction culture, (4) multiplication culture, (5) strong seedling culture, (6) ex-vitro rooting seedling transplanting and the like. The rapid propagation method solves the problems that Chinese fir tissue culture seedlings have unstable genetic quality and initial buds are difficult to induce, prone to browning and free of rooting, meanwhile, the procedures of rooting, seedling hardening and transplanting are simplified, so that the culture period is shortened, and the breeding cost is reduced.

The invention discloses a quick root accelerating method for Tamarix taklamakanensis M. T. Liu cutting slips. The method comprises the steps of collection and cutting of the cutting slips, rooting induction of the cutting slips, root accelerating culture in a hotbed, cuttage of the cutting slips and the like. According to the quick root accelerating method for the Tamarix taklamakanensis M. T. Liucutting slips, the structure of the root accelerating hotbed is reasonably designed, rooting induction and root accelerating in the hotbed are conducted by selecting appropriate rooting powder, and the root accelerating temperature and time are reasonably controlled, so that appropriate environmental conditions are created for the Tamarix taklamakanensis M. T. Liu cutting slips, and the coalescence and rooting of the cutting slip base portions are effectively accelerated; compared with a traditional Tamarix taklamakanensis M. T. Liu cuttage method, the cutting slip surviving rate is increasedby 10%, and the cutting slip breeding efficiency and the cuttage seedling-raising survive rate are obviously improved; the false-growth phenomenon occuring when the Tamarix taklamakanensis M. T. Liucutting slips germinate and grow shoots is effectively controlled, and the problems that the Tamarix taklamakanensis M. T. Liu cutting slip cuttage is difficult in rooting and low in breeding coefficient are solved.

The invention provides a tissue culture propagation method for helleborus thibetanus. The tissue culture propagation method comprises the following steps of (1), obtaining and treating an explant; (2), carrying out adventitious bud induction culture; (3), carrying out adventitious bud subculture multiplication culture; (4), rooting culture; and (5), hardening seedlings and transplanting to obtain helleborus thibetanus seedlings. According to the method, hormone and a culture environment are comprehensively adjusted, so that the proliferation multiple of the adventitious buds of the helleborus thibetanus reaches 3.5 or above, the rooting rate reaches 90% or above, and the problems that the helleborus thibetanus is low in propagation coefficient, difficult to root and long in production period are solved; and besides, by keeping the excellent characters of the excellent strains, seedlings with stable excellent characters can be produced, standardization and factory operation are easy, and the excellent seedlings with the unified standard are provided for the market.

The invention discloses nutrition soil for cuttage of European rosa chinensis. The nutrition soil is prepared from the following raw materials in percentage by mass: 40 to 60 percent of acidic peat soil, 5 to 15 percent of perlite, 10 to 20 percent of vermiculite, 1 to 5 percent of rotten chicken manure, 1 to 5 percent of bone meal, 5 to 15 percent of rooting powder and 5 to 10 percent of insecticide sterilizing agents. The nutrition soil is loose and breathable; the nutrition ingredient content is high; through the addition of the bone meal, the seedling strengthening effect is achieved; thenutrition is accumulated for the early-stage flower bud differentiation; while the water is preserved, the water discharge is facilitated; the rooting requirements of the European rosa chinensis are met. The difficult problems of rooting difficulty, weak generated roots, survival difficulty in the later stage and the like of the European rosa chinensis after the cuttage can be solved.