A kind of rooting medium of meranti and its application
A rooting medium and rooting culture technology, applied in application, plant regeneration, climate change adaptation, etc., to achieve large-scale and factory production, neat and robust growth, and good reproducibility.
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[0053] Take the tissue culture multiplication seedlings growing more than 2.5cm in a sterile tissue culture bottle, cut off the callus at the base on the ultra-clean workbench, and transfer it to a sterile tissue culture bottle that has been added with rooting medium. The tissue culture bottle is a 240mL glass tissue culture bottle, and the volume of the rooting medium added is 30mL. The formulation of the rooting medium includes: improved MS+IBA0.3mg / L (final concentration)+3-AB0.1mg / L (final concentration), and the pH is 5.8. The cultivation conditions are as follows: the cultivation temperature is 23° C., the light time is 16 hours per day; the dark time is 8 hours, the light intensity is 1800 lx, and the rooting culture is 25 days. The rooted seedlings of Eucalyptus meranti after rooting culture were as figure 2 shown.
[0054] The above-mentioned comparative examples 1 and 2, and the experimental results of inducing meranti rooting in embodiment 1 are as shown in table...
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