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A kind of rooting medium of meranti and its application

A rooting medium and rooting culture technology, applied in application, plant regeneration, climate change adaptation, etc., to achieve large-scale and factory production, neat and robust growth, and good reproducibility.

Inactive Publication Date: 2018-10-02
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to relevant literature, it is found that there is no precedent for this substance to be used in rooting medium formulations

Method used

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  • A kind of rooting medium of meranti and its application
  • A kind of rooting medium of meranti and its application
  • A kind of rooting medium of meranti and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Take the tissue culture multiplication seedlings growing more than 2.5cm in a sterile tissue culture bottle, cut off the callus at the base on the ultra-clean workbench, and transfer it to a sterile tissue culture bottle that has been added with rooting medium. The tissue culture bottle is a 240mL glass tissue culture bottle, and the volume of the rooting medium added is 30mL. The formulation of the rooting medium includes: improved MS+IBA0.3mg / L (final concentration)+3-AB0.1mg / L (final concentration), and the pH is 5.8. The cultivation conditions are as follows: the cultivation temperature is 23° C., the light time is 16 hours per day; the dark time is 8 hours, the light intensity is 1800 lx, and the rooting culture is 25 days. The rooted seedlings of Eucalyptus meranti after rooting culture were as figure 2 shown.

[0054] The above-mentioned comparative examples 1 and 2, and the experimental results of inducing meranti rooting in embodiment 1 are as shown in table...

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Abstract

The invention belongs to the fields of forestry biotechnological seed breeding or plant cell engineering and discloses an E.urophylla*E.saligna rooting culture medium and an application thereof. On the basis of an improved MS culture medium, IBA and 3-AB are supplied to the rooting culture medium. By means of addition of the novel rooting inductive substance 3-AB and the synergistic effect of the rooting-type growth regulator IBA, rooting ratio of the E.urophylla*E.saligna is more than 87% and transplanting survival rate is more than 91%. When rooted seedlings are transplanted on bags, the seedlings grow tidily and robustly, have strong resistance, and completely reach demands of large-scale and commercial seedling production. The rooting culture medium has good repeatability and stable effects, and has no intensive fluctuation on the rooting ratio and transplanting survival rate even after multiple times of usage, thereby completely solving a problem that the E.urophylla*E.saligna is difficult to root during tissue culture induction.

Description

technical field [0001] The invention belongs to the field of forestry biotechnology breeding or plant cell engineering, and particularly relates to a rooting medium of meranti and its application. Background technique [0002] Eucalyptus saligna is a tall evergreen tree of the genus Eucalyptus L'Herit. Its original distribution area is located in southern Queensland and parts of New South Wales, Australia. The practice of introducing eucalyptus in Fujian and the research of Tao Desheng (1997) showed that the meranti introduced to China has good early fast-growing characteristics, adaptability, frost resistance and poor soil resistance, and can grow in subtropical forest areas. The development potential is huge. In addition, Eucalyptus meranti has thin and narrow leaves, a small crown, and strong typhoon resistance, which is of great significance for the selection of typhoon-resistant commercial forest species in the southeast coast of China. [0003] The eucalyptus urophyl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001Y02A40/22Y02P60/40
Inventor 韩超
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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