Eucalyptus urophylla rooting medium and application

A rooting medium and rooting culture technology, applied in the application, plant regeneration, climate change adaptation and other directions, to achieve stable effect, realize large-scale and factory production, and solve the problem of difficult rooting.

Inactive Publication Date: 2017-01-25
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After reviewing the relevant literature, it was found that there is no precedent for this substance to be used in the formula of rooting medium

Method used

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  • Eucalyptus urophylla rooting medium and application
  • Eucalyptus urophylla rooting medium and application
  • Eucalyptus urophylla rooting medium and application

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Take tissue culture proliferation seedlings that grow in a sterile tissue culture bottle with a height of more than 2.5 cm, cut off the callus at the base on a clean bench, and transfer to a sterile tissue culture bottle that has been added with rooting medium. The tissue culture bottle is a 240 mL glass tissue culture bottle, and the volume of rooting medium added is 30 mL. The formula of rooting medium includes: modified MS+IBA0.5mg / L (final concentration)+ABT-11.5mg / L (final concentration)+3-AB 0.1mg / L (final concentration), and the pH is 5.8. The culture conditions were culture temperature of 23°C, daily light time of 16h, dark time of 8h, light intensity of 1800lx, and rooting culture for 25 days. Eucalyptus urophylla rooted seedlings such as figure 2 Shown.

[0054] The rooting results of Example 1 and Comparative Examples 1 to 2 are shown in Table 1. 100 plants were sampled, and the calculation formula for each index is as follows:

[0055] Rooting rate (%)=number ...

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Abstract

The invention belongs to the field of forestry biotechnology breeding and discloses a eucalyptus urophylla rooting medium and application. According to the rooting medium, root induction substances, such as IBA, ABT-1, 3-AB and 3-AB, are added on the basis of an improved MS culture medium formula so as to serve as the conventional cytostatics, and by matching with the other two root induction substances, 3-AB achieves an obvious effect of inducing adventitious roots, so that the growth and development directions of cambial cells are changed, a parenchymal cell state is developed into a root primordium form of the adventitious roots, and the cells are decreased and increased, continuously divided along the periclinal direction and finally break through the epidermis to grow into lateral roots. According to use of the rooting medium disclosed by the invention, the rooting rate of eucalyptus urophylla can reach over 85%. The survival rate can be over 90% after transplanting. Moreover, the rooting medium is high in reproducibility and stable in effect, and after repeated use of the medium, the rooting rate and transplanting survival rate do not have great fluctuation.

Description

Technical field [0001] The invention belongs to the field of forestry biotechnology breeding, and particularly relates to a rooting culture medium for Eucalyptus tail and its application. Background technique [0002] Eucalyptus benthamii (Eucalyptus benthamii) is a tall tree of the Eucalyptus genus Symphomyrtus. Its natural distribution is limited to the area of ​​100km long and 40km wide along the Naping River in southwestern Sydney. Eucalyptus Bentham has shown strong adaptability in the introduction trials in my country. It has excellent characteristics such as fast-growing, cold-tolerance, and high-temperature resistance. It is an excellent Eucalyptus species suitable for promotion in mid-subtropical regions. [0003] The Eucalyptus urophylla × E. benthamii used in the present invention is an artificial hybrid of Eucalyptus urophylla × E. benthamii as its female parent and Eucalyptus Benthamii as its male parent. It is suitable for use in cold areas on the northern and souther...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02A40/22Y02P60/40
Inventor 韩超
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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