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A kind of rooting culture medium of Eucalyptus caudatum and its application

A rooting medium and rooting culture technology, applied in the application, plant regeneration, climate change adaptation and other directions, to achieve neat and robust growth, large-scale and factory production, and good repeatability.

Inactive Publication Date: 2018-11-06
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After reviewing the relevant literature, it was found that there is no precedent for this substance to be used in the formula of rooting medium

Method used

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  • A kind of rooting culture medium of Eucalyptus caudatum and its application
  • A kind of rooting culture medium of Eucalyptus caudatum and its application
  • A kind of rooting culture medium of Eucalyptus caudatum and its application

Examples

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Embodiment 1

[0053] Take the tissue culture multiplication seedlings growing more than 2.5cm in a sterile tissue culture bottle, cut off the callus at the base on the ultra-clean workbench, and transfer it to a sterile tissue culture bottle that has been added with rooting medium. The tissue culture bottle is a 240mL glass tissue culture bottle, and the volume of the rooting medium added is 30mL. The formulation of the rooting medium includes: improved MS+IBA0.5mg / L (final concentration)+ABT-11.5mg / L (final concentration)+3-AB 0.1mg / L (final concentration), and the pH is 5.8. The cultivation conditions were as follows: cultivation temperature 23° C., 16 hours of light time per day, 8 hours of darkness time, light intensity of 1800 lx, and rooting culture for 25 days. The rooting shoots of Eucalyptus caudatum after rooting culture are as figure 2 shown.

[0054] The rooting result of embodiment 1 and comparative examples 1~2 is as shown in table 1, and sampling all gets 100 strains, and ...

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Abstract

The invention belongs to the field of forestry biotechnology breeding and discloses a eucalyptus urophylla rooting medium and application. According to the rooting medium, root induction substances, such as IBA, ABT-1, 3-AB and 3-AB, are added on the basis of an improved MS culture medium formula so as to serve as the conventional cytostatics, and by matching with the other two root induction substances, 3-AB achieves an obvious effect of inducing adventitious roots, so that the growth and development directions of cambial cells are changed, a parenchymal cell state is developed into a root primordium form of the adventitious roots, and the cells are decreased and increased, continuously divided along the periclinal direction and finally break through the epidermis to grow into lateral roots. According to use of the rooting medium disclosed by the invention, the rooting rate of eucalyptus urophylla can reach over 85%. The survival rate can be over 90% after transplanting. Moreover, the rooting medium is high in reproducibility and stable in effect, and after repeated use of the medium, the rooting rate and transplanting survival rate do not have great fluctuation.

Description

technical field [0001] The invention belongs to the field of forestry biotechnology breeding, and in particular relates to a rooting medium for Eucalyptus urophylla and its application. Background technique [0002] Eucalyptus benthamii is a tall tree of the subgenus Symphomyrtus of the genus Eucalyptus, and its natural distribution is limited to the area 100km long and 40km wide along the Napin River in southwest Sydney. Eucalyptus benthamum has shown strong adaptability in the introduction test in my country, and has excellent characteristics such as fast growth, cold resistance, and high temperature resistance. It is an excellent Eucalyptus species that is more suitable for promotion in mid-subtropical regions. [0003] The used Eucalyptus urophylla (E.urophylla×E.benthamii) of the present invention is that female parent is Eucalyptus urophylla and male parent is the artificial hybrid of Eucalyptus benthamii, is suitable in the low mountains and the cold areas of the nort...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008Y02A40/22Y02P60/40
Inventor 韩超
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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