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A method for rooting in a bottle of tissue cultured seedlings of Xianmai fragrant tea

A technology of showing pulse-flavored tea vegetables and tissue culture seedlings, applied in the field of plant tissue culture, can solve the problems of low rooting rate of tissue culture seedlings, insufficient supply of propagation methods, and large demand, so as to promote industrial production processes and reduce production costs , the effect of increasing the rooting rate

Active Publication Date: 2022-04-01
WUXI MISHO ECOLOGY LANDSCAPE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rooting rate of tissue culture seedlings is not high, resulting in a large demand in the market, and the traditional propagation methods are in short supply, so tissue culture is needed to reproduce in large quantities

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for rooting in a bottle of tissue cultured seedlings of Xianmaixiang tea leaves, comprising the steps of:

[0019] (1) Select the subcultured Xianmaixiang tea vegetable tissue culture seedlings, and the selection standard is healthy Xianmaixiang tea vegetable seedlings with a height of 3-4cm, normal growth and no vitrification;

[0020] (2) Inoculate the selected subcultured Xianmaixiangcha seedlings on 1 / 4MS rooting medium;

[0021] The rooting medium includes 40 g / L of sucrose, 7 g / L of agar powder, and added indole-3-butyric acid with a concentration of 0.1 mg / L; the pH of the medium is 6.0, the culture temperature is 26 ° C, and the light is 3000 lx .

[0022] The root primordium grew out after 9 days of inoculation, and the root length was 3-4cm on the 17th day, and the statistical rooting rate was 95% (the rooting rate was the percentage of the number of rooted plants and the number of plants in the inoculated group).

Embodiment 2

[0024] A method for rooting in a bottle of tissue cultured seedlings of Xianmaixiang tea leaves, comprising the steps of:

[0025] (1) Select the subcultured Xianmaixiang tea vegetable tissue culture seedlings, and the selection standard is healthy Xianmaixiang tea vegetable seedlings with a height of 3-4cm, normal growth and no vitrification;

[0026] (2) Inoculate the selected subcultured Xianmaixiangcha seedlings on 1 / 4MS rooting medium;

[0027] The rooting medium comprises 35 g / L of sucrose, 6 g / L of agar powder, and added indole-3-butyric acid with a concentration of 0.2 mg / L; the pH of the medium is 5.9, the culture temperature is 25 ° C, and the light is 3000 lx .

[0028] Root primordium grew out after 8 days of seedling inoculation, and the root length was 3-4 cm on 16 days, and the statistical rooting rate was 96% (the rooting rate was the percentage of the number of rooted plants and the number of plants in the inoculation group).

Embodiment 3

[0030] A method for rooting in a bottle of tissue cultured seedlings of Xianmaixiang tea leaves, comprising the steps of:

[0031] (1) Select the subcultured Xianmaixiang tea vegetable tissue culture seedlings, and the selection standard is healthy Xianmaixiang tea vegetable seedlings with a height of 3-4cm, normal growth and no vitrification;

[0032] (2) Inoculate the selected subcultured Xianmaixiangcha seedlings on 1 / 4MS rooting medium;

[0033] The rooting medium includes 30 g / L of sucrose, 5 g / L of agar powder, and the addition of indole-3-butyric acid with a concentration of 0.3 mg / L; the pH of the medium is 5.8, the culture temperature is 24 ° C, and the light is 2500 lx .

[0034] After 5 days of inoculation, the root primordium grew out, and the root length was 3 to 4 cm on the 13th day, and the statistical rooting rate was 100% (the rooting rate was the percentage of the number of rooted plants and the number of plants in the inoculation group).

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Abstract

The invention belongs to the technical field of plant tissue culture, and in particular relates to a rooting method in a bottle for tissue cultured seedlings of Xianmaixiang tea. The method for rooting in a bottle of the tissue-cultured plantlets of Xianmaixiang tea vegetables of the present invention, the steps are as follows: (1) Select the tissue culture seedlings of Xianmaixiang tea vegetables subcultured, the selection standard is a height of 3-4cm, normal growth and no glass (2) inoculate the selected subcultured Xianmaixiang tea seedlings on 1 / 4MS rooting medium; add growth hormone to the 1 / 4MS medium. The present invention determines the optimal rooting medium through a large number of experiments, can effectively solve the rooting problem of Xianmaixiang tea vegetable, and significantly improves the rooting rate of rooting in the tissue culture bottle of Xianmaixiang tea vegetable, and the rooting rate is as high as 95%. The above, while reducing the production cost, can provide the market with a large number of high-quality tissue culture seedlings to meet the growing demand of the market, and then effectively promote the industrialized production process.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rooting method in a bottle for tissue cultured seedlings of Xianmaixiang tea. [0002] technical background [0003] Xianmai Xiangchacai is also called Big Leaf Snake Manager, Blue Flower Bupleurum, Maiye Xiangchacai, etc. It belongs to Labiatae, Lamiaceae, Basil subfamily, and Xiangchacai family species. The whole herb is used as medicine, collected in summer and autumn, washed and used fresh or dried in the sun. The taste is slightly pungent, bitter, and cold. Impetigo, eczema, itchy skin. [0004] Xianmai coriander and tea vegetables are mainly propagated by seeds. The quantity is small and the excellent characteristics of the excellent varieties will decline after sexual reproduction. In order to maintain the excellent characteristics of the varieties, asexual reproduction is required. The rooting rate of tissue culture seedlings is not high, resul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 周敬锋李懿玮王钰涵
Owner WUXI MISHO ECOLOGY LANDSCAPE
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