Artificial efficient propagation method of swertia nervosa (G.Don)Wall.

Abstract

The invention discloses an artificial efficient propagation method of swertia nervosa (G.Don)Wall.. According to the method, in the same culture medium, callus induction and basal stem multiple shootgeneration can be conducted simultaneously, basal stem multiple shoot generation and multiplication culture can also be conducted simultaneously, and thus the culture procedure is simplified into three steps; in the whole rapid propagation process, only three kinds of culture mediums are needed to achieve the purposes of callus induction, basal stem multiple shoot generation, and multiplication culture, avoid the phenomena of browning and vitrification and solve the problem of root taking, the inoculation procedures and the culture space are saved, and arrangement of production plans is facilitated; besides, since the inoculation frequency is reduced, the possibility of contamination is lowered; the culture mediums for callus induction and multiplication culture have the function of basalstem multiple shoot generation, so that biological materials better adapt to replacement of the culture mediums, the success rate of tissue culture of the biological materials is increased, and the propagation coefficient is greatly increased.

Description

Technical field: [0001] The invention relates to the propagation technology of Chinese herbal medicine, in particular to an artificial high-efficiency propagation method of Swertia japonica. Background technique: [0002] Swertia nervosa (G.Don) Wall. is a one-year or biennial herb of the genus Swertia in the Gentianaceae (Gentianaceae), also known as Hepatitis Grass, Four-edge Grass, Mountain Coptis, Herring Grass etc., mainly distributed in Southwest my country-Himalayan region, growing in river beaches, hillsides, sparse forests and bushes at an altitude of 460-2700m. Swertia japonica is a national medicine commonly used by the Tujia people in the southwest region. Its chemical components are mainly swertiamarin, gentiopicroside, oleanolic acid and 1,7-dihydroxy- 3,8-dimethoxyketone and other ketones have the functions of clearing heat and detoxification, clearing liver and promoting gallbladder, sedative and analgesic, etc. It has unique curative effect on the treatment ...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for artificial high-efficiency propagation of Swertia vegetativeis, in the same medium, the present invention can simultaneously induce callus and basal shoot clustering, and simultaneously carry out basal tuber clustering bud generation and proliferation Cultivation, simplifies the cultivation procedure to three steps; the entire rapid propagation process only needs three kinds of medium to solve the problems of callus, basal shoot clustering bud generation and proliferation, overcoming browning and vitrification, and rooting, saving inoculation The program and culture space are conducive to the arrangement of production plans; in addition, because the number of inoculations is reduced, the possibility of contamination is reduced, and the medium for callus induction and proliferation culture has the function of basal shoot clustering, which makes the replacement of biological materials The culture medium is easier to adapt, thereby improving the success rate of tissue culture of biological materials and greatly improving the reproduction coefficient

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