The invention discloses a medicine composition and a preparation with the functions of reducing blood pressure and blood sugar and protecting liver and a preparation method of the medicine composition. The medicine composition is prepared from, by weight, 80-120 parts of negative ion small-molecule water, 0.15-0.25 part of agarotetrols, 50-70 parts of tea polyphenol, 40-60 parts of tea polysaccharide, 50-70 parts of tea pigments, 80-100 parts of total flavone and 5-25 parts of black carp gallbladders. The medicine composition has remarkable functions of reducing blood sugar and urine sugar, symptoms of high blood pressure, high blood sugar and high blood fat of diabetes mellitus can be effectively improved, blood sugar and blood pressure are effectively reduced, blood fat can be effectively reduced, unconformable symptoms caused by the high blood pressure are improved, cholesterol and beta-lipoprotein in blood serum are reduced, insulin secretion is facilitated, immunity is enhanced, blood flow volume is adjusted, atherosclerosis is prevented, the medicine composition resists bacterium, diminishes inflammation, clears heat, removes toxicity, facilitates feces excretion and diuresis, removes moisture, enhances liver metabolism, prevents cancers, restrains tumor growth, reduces the blood sugar, the blood pressure, the cholesterol and the blood fat and has unique and miraculous effect on the diabetes mellitus and complications of the diabetes mellitus, and a synergistic balance adjustment function is achieved.