The invention discloses a malt-containing sour-sweet nutritional beverage and a preparation method thereof. The beverage is prepared from the following raw materials, by weight, 40-60 parts of malt, 15-24 parts of wheat seedling, 20-30 parts of prune, 13-18 parts of skimmed milk powder, 0.02-0.04 parts of a lactic acid bacterium starter, 15-25 parts of mulberry leaf, 10-20 parts of fresh orange peel, 2-3 parts of peanut shell, 2-3 parts of Rosa xanthina Lindl, 1-2 parts of Panax Tseng, 2-3 parts of Mongolian oak leaf, 10-20 parts of tender pepper leaf, 8-12 parts of royal jelly, 6-8 parts of egg white, 10-12 parts of rock sugar, an appropriate amount of rice wine, 4-6 parts of a lemon juice and 6-12 parts of a nutrition liquid. The malt-containing sour-sweet nutritional beverage prepared in the invention has the characteristics of sour-sweet-smooth mouthfeel, rich and balanced nutrition, and easy digestion and absorption by human bodies after fermentation, has the effects of liver heat clearing, eyesight improving and stomach strengthening due to the addition of mulberry leaf, tender pepper leaf and other nutrients, has a synergistic effect realized by the nutrients and other traditional Chinese medicinal components, and can improve spleen deficiency, reduced appetite, indigestion, flatulence, stomach cold and other adverse symptoms.