1 results about "Sterculia" patented technology

The application provides a fat sea base gene and adulteration identification method based on multi-gene joint DNA barcoding, four gene joint barcode combinations are formed by adopting nuclear gene ITS2 and chloroplast genes matK, rbcL and psbA-trnH, at least two pairs of optimized alternative primers for each target gene and fat sea genus specific preliminary screening primer Ster-1 are matched, genus level rapid preliminary screening is realized to exclude non-fat sea genus samples and shorten the identification process, and the efficient amplification success rate of multi-gene fragments is ensured through the flexible use of alternative primers; fat sea and round fat sea, Sterculia and mixed adulterants can be accurately distinguished, the problems that traditional identification methods are subjective and species with close genetic relationship are difficult to distinguish are effectively solved; the method is not limited by sample morphology and fragmentation degree, can be widely applied to the true and false identification, quality control and traceability of traditional Chinese medicinal materials fat sea and its products, and provides technical support for standardizing market order and ensuring the safety and effectiveness of clinical medication.