6 results about "Phagocytic index" patented technology

The invention relates to the technical field of collagen peptides, in particular to a collagen peptide capable of enhancing immunity and a preparation method thereof.The preparation method comprises the steps that squid skin is subjected to enzymolysis under the alkaline condition to obtain enzymatic hydrolysate, pitch-based activated carbon is added into the enzymatic hydrolysate for decoloration and deodorization, and then low-temperature freeze drying is conducted. The collagen peptide disclosed by the invention can effectively improve spleen and thymus indexes of mice, remarkably improve the phagocytic rate and phagocytic index of macrophages, play a role in enhancing immunity, and have wide application prospects in the fields of health foods and medicines.

The invention belongs to the technical field of microbial engineering, and particularly relates to leuconostoc mesenteroides subsp. Mesenteroides YWN-1 with the effects of benefiting qi, nourishing blood and enhancing immunity and application of the leuconostoc mesenteroides subsp. Mesenteroides YWN-1. The preservation number of the leuconostoc mesenteroides subsp. Mesenteroides YWN-1 is CGMCC No.34608. The leuconostoc mesenteroides subsp. Mesenteroides YWN-1 can significantly improve immune organ thymus and spleen injuries caused by deficiency of qi and blood, increase the number of red blood cells, hemoglobin and platelets of mice with deficiency of qi and blood, and have the efficacy of improving deficiency of both qi and blood. The phagocytic index, the serum hemolysin content, the half hemolysis value and the immune factor IgG and IgM content of mice with low immunity are improved, and the effects of improving immunity and resistance are achieved.

The invention discloses a mouse peritoneal macrophage phagocytosis experiment method. The method comprises the following steps: step 1, reagent preparation and SPF-level Kunming mouse preparation; 2, macrophage induction: injecting a 6% soluble starch solution into the abdominal cavity of the mouse; 3, inducing phagocytic reaction, namely injecting 1% chicken erythrocyte suspension into the abdominal cavity 48 hours after injecting the 6% soluble starch solution; 4, collecting a sample, injecting the 1% chicken red blood cell suspension for 30 minutes, and taking peritoneal fluid; 5, preparing and dyeing a smear, and clearly distinguishing cell morphology; and 6, carrying out microscopic examination and quantitative analysis, and evaluating the activity of the macrophages by counting the phagocytic percentage and the phagocytic index. The method solves the problems of disordered parameters, difficulty in dyeing identification, extensive results and the like in a traditional method, is simpler and more convenient to operate and higher in result stability, and can provide reliable technical support for immunological research, drug development and disease assessment so as to guarantee the accuracy of experimental data.

The application discloses an immunoregulation compound of polygonum multiflorum-Thunb and longan oligosaccharide and a preparation and application thereof, and belongs to the technical field of plant active ingredient extraction. In view of the problems of poor immunoregulation effect caused by phase separation, asynchronous absorption and insufficient synergistic activation of the existing compounding method, the application realizes conformation matching of the polygonum multiflorum oligosaccharide (beta-1, 3-glucan) and the longan oligosaccharide (alpha-1, 6-galacturonic acid polysaccharide) through process innovation such as composite enzymolysis (proportioned synergistic action of cellulase and pectinase), online continuous mixing and raw material pretreatment, promotes in-vivo absorption synchronism and synergistically activates the TLR4 / MyD88 immune pathway. The prepared compound can significantly improve the concentration of immune factors such as IL-6, TNF-alpha and IFN-gamma in serum, enhance the phagocytosis rate, phagocytosis index and CD86+ cell proportion of macrophages, and realizes efficient immunoregulation. The compound can be widely applied to functional food, nutritional supplements and immunoregulation drugs, and provides a new solution for improving the immune response of the body.

This invention provides a method for preparing collagen peptides and their application in enhancing immunity. Furthermore, this invention screened and isolated a collagen peptide MY-4 with good antioxidant properties, which exhibits a strong protective effect against thymic lymphocytes. The peptide can effectively increase the phagocytic rate and phagocytic index of macrophages. Therefore, the collagen peptides of this invention can effectively enhance immunity and have promising application prospects.

The invention discloses an immunoregulation compound of polygonum multiflorum-longan oligosaccharide as well as preparation and application of the immunoregulation compound, and belongs to the technical field of plant active ingredient extraction. In order to solve the problem of poor immunoregulation effect caused by asynchronous phase separation and absorption and insufficient synergistic activation in the existing compounding method, the method disclosed by the invention comprises the following steps: innovating processes such as composite enzymolysis (cellulase and pectinase synergistically act in proportion), online continuous mixing and raw material pretreatment; according to the invention, the radix polygoni multiflori oligosaccharide (beta-1, 3-glucan) and the longan oligosaccharide (alpha-1, 6-galacturonidan) are subjected to conformation matching, so that the in-vivo absorption synchronism is promoted, and the TLR4 / MyD88 immune pathway is synergistically activated. The prepared compound can significantly improve the concentration of immune factors such as IL-6, TNF-alpha and IFN-gamma in serum, enhance the phagocytic rate and phagocytic index of macrophages and the proportion of CD86 + cells, and achieve efficient immunoregulation. The compound can be widely applied to functional foods, nutritional supplements and immunomodulatory drugs, and a new solution is provided for improving the immune response of the organism.