3 results about "Phagocytic index" patented technology

The invention relates to the technical field of collagen peptides, in particular to a collagen peptide capable of enhancing immunity and a preparation method thereof.The preparation method comprises the steps that squid skin is subjected to enzymolysis under the alkaline condition to obtain enzymatic hydrolysate, pitch-based activated carbon is added into the enzymatic hydrolysate for decoloration and deodorization, and then low-temperature freeze drying is conducted. The collagen peptide disclosed by the invention can effectively improve spleen and thymus indexes of mice, remarkably improve the phagocytic rate and phagocytic index of macrophages, play a role in enhancing immunity, and have wide application prospects in the fields of health foods and medicines.

The invention discloses a mouse peritoneal macrophage phagocytosis experiment method. The method comprises the following steps: step 1, reagent preparation and SPF-level Kunming mouse preparation; 2, macrophage induction: injecting a 6% soluble starch solution into the abdominal cavity of the mouse; 3, inducing phagocytic reaction, namely injecting 1% chicken erythrocyte suspension into the abdominal cavity 48 hours after injecting the 6% soluble starch solution; 4, collecting a sample, injecting the 1% chicken red blood cell suspension for 30 minutes, and taking peritoneal fluid; 5, preparing and dyeing a smear, and clearly distinguishing cell morphology; and 6, carrying out microscopic examination and quantitative analysis, and evaluating the activity of the macrophages by counting the phagocytic percentage and the phagocytic index. The method solves the problems of disordered parameters, difficulty in dyeing identification, extensive results and the like in a traditional method, is simpler and more convenient to operate and higher in result stability, and can provide reliable technical support for immunological research, drug development and disease assessment so as to guarantee the accuracy of experimental data.

This invention provides a method for preparing collagen peptides and their application in enhancing immunity. Furthermore, this invention screened and isolated a collagen peptide MY-4 with good antioxidant properties, which exhibits a strong protective effect against thymic lymphocytes. The peptide can effectively increase the phagocytic rate and phagocytic index of macrophages. Therefore, the collagen peptides of this invention can effectively enhance immunity and have promising application prospects.