The invention relates to a method for preparing
hyriopsis cumingii polysaccharide, which comprises following steps: crushing
hyriopsis cumingii, extracting with hot water, filtering with 60-100 orders, condensing filter liquor, then, adding
ethanol to deposit, re-dissolving deposition with hot water, adjusting the pH value to be 7-10, adding remixed proteinase 0.3%-0.6% according to a
protein weight gage in re-dissolved materials, carrying out
enzymatic hydrolysis for 4-8 hours under the temperature of 30-60DEG C, wherein components of the remixed remixed proteinase comprise:
alkaline protease (Alcalase) and compound
protease (Protamex) which are mixed equivalently, killing
enzyme for the materials after the
enzymatic hydrolysis under the temperature of 80-90DEG C, using the
ethanol to deposit for the second time,
drying the deposition in the vacuum, crushing over 60 orders, and obtaining products of the
hyriopsis cumingii polysaccharide. Experiments show that the hyriopsis cumingii
polysaccharide which is prepared by the method of the invention has the ability to induce the
mouse spleen lymphocytes to proliferate, the hyriopsis cumingii polysaccharide can increase the volume value of mice serum
hemolysin and can increase the phagocytic rate and the
phagocytic index of chicken-red cells
phagocytosis in mouse peritoneal, and the hyriopsis cumingii polysaccharide has a pluralityof
biological activity and has the application prospect as medicinal resources and
healthy food.