In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling

A technology for tissue culture seedlings and salt tolerance is applied in the field of in-bottle rooting of salt-tolerant white elm tissue culture seedlings, which can solve the problems of low survival rate, difficult rooting and high cost.

Active Publication Date: 2013-10-30
上海菁艺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] White elm is generally propagated by seeds, but the good characteristics of good varieties will decline after sexual reproduction. In order to better maintain the good characteristics of the variety, asexual reproduction must be carried out, and the good characteristics of the variety must be maintained by means of grafting, but the cost Higher and lower survival rate
[0004] At the same time, because the white elm has a certain salt-alkali tolerance, rooting is more difficult, and the rooting rate is low, resulting in the current situation that the supply of salt-tolerant white elm seedlings is less than the demand in the nursery stock market.

Method used

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  • In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling
  • In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling
  • In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The subcultured salt-tolerant elm tissue culture seedlings were selected, and the selection standard was a single elm seedling with a height of 2-3.5cm, robustness, and stretched leaves;

[0039] The salt-tolerant white elm tissue culture seedling of selected subculture is inoculated on the WPM rooting medium;

[0040] Wherein, the WPM rooting medium is a 1 / 2 WPM rooting medium which is reduced by 1 / 2 with a large amount of elements in the WPM basic medium.

[0041] The auxin is indole butyric acid (IBA), and its final concentration is 0.05 mg / L; the cytokinin is naphthalene acetic acid (NAA), and its final concentration is 0.05 mg / L; the exogenous addition The substances are activated carbon (AC) and white sugar, the final concentration of the activated carbon (AC) is 0.2g / L, and the final concentration of the white sugar is 20g / L.

[0042] The pH value of the WPM rooting medium was 5.8.

[0043] Twenty-five days after inoculation, the rooting rate was counted.

Embodiment 2

[0045] The subcultured salt-tolerant elm tissue culture seedlings were selected, and the selection standard was a single elm seedling with a height of 2-3.5cm, robustness, and stretched leaves;

[0046] The salt-tolerant white elm tissue culture seedling of selected subculture is inoculated on the WPM rooting medium;

[0047] Wherein, the WPM rooting medium is a 1 / 2 WPM rooting medium which is reduced by 1 / 2 with a large amount of elements in the WPM basic medium.

[0048] The auxin is indole butyric acid (IBA), and its final concentration is 0.2 mg / L; the cytokinin is naphthalene acetic acid (NAA), and its final concentration is 0.1 mg / L; the exogenous addition The substances are activated carbon (AC) and white sugar, the final concentration of the activated carbon (AC) is 0.5g / L, and the final concentration of the white sugar is 30g / L.

[0049] The pH value of the WPM rooting medium was 5.85.

[0050] Twenty-five days after inoculation, the rooting rate was counted.

Embodiment 3

[0052] The subcultured salt-tolerant elm tissue culture seedlings were selected, and the selection standard was a single elm seedling with a height of 2-3.5cm, robustness, and stretched leaves;

[0053] The salt-tolerant white elm tissue culture seedling of selected subculture is inoculated on the WPM rooting medium;

[0054] Wherein, the WPM rooting medium is a 1 / 2 WPM rooting medium which is reduced by 1 / 2 with a large amount of elements in the WPM basic medium.

[0055] The auxin is indole butyric acid (IBA), and its final concentration is 0.3 mg / L; the cytokinin is naphthalene acetic acid (NAA), and its final concentration is 0.2 mg / L; the exogenous addition The substances are activated carbon (AC) and white sugar, the final concentration of the activated carbon (AC) is 2g / L, and the final concentration of the white sugar is 40g / L.

[0056] The pH value of the WPM rooting medium was 5.9.

[0057] Twenty-five days after inoculation, the rooting rate was counted.

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Abstract

The purpose of the present invention is to disclose an in-bottle rooting method for a salt tolerance ulmus pumila tissue culture seedling. The method comprises the following steps: (1) selecting a secondary seedling, wherein a secondary culture salt tolerance ulmus pumila tissue culture seedling is selected, the height of the seedling is 2-3.5 cm, and the robust individual ulmus pumila seedling having extending leaves is selected; and (2) inoculating the selected secondary culture salt tolerance ulmus pumila tissue culture seedling onto a WPM rooting medium, wherein the WPM rooting medium is added with growth hormone, cytokinin and exogenous additives. According to the present invention, a medium formula having a high rooting rate is adopted so as to effectively solve the rooting problem of the salt tolerance ulmus pumila tissue culture seedling, improve the rooting rate of the salt tolerance ulmus pumila tissue culture seedling, reduce the cost, accelerate the factory production of the salt tolerance ulmus pumila, provide high quality salt tolerance ulmus pumila seedlings for nursery stock markets, and achieve the purpose of the present invention, wherein the rooting rate of the salt tolerance ulmus pumila tissue culture seedling can be up to 95%.

Description

technical field [0001] The invention relates to a method for rooting in a bottle for cultivating seedlings, in particular to a method for rooting in a bottle for a salt-tolerant white elm tissue culture seedling. Background technique [0002] White elm, also known as elm, is a deciduous tree of the family Elm, which is widely distributed and is mainly produced in the north temperate zone. White elm is light, drought-tolerant, cold-resistant, barren-resistant, does not choose soil, has strong adaptability, and is mostly used for afforestation. Elm wood is wear-resistant and rot-resistant, and is an excellent wood for shipbuilding, construction, interior decoration floors and furniture. The bark fiber is strong and can be used as raw material for rayon and paper making. The leaves contain starch and protein and can be used as feed. The skin, leaves and fruits can be used as medicine, and the seeds can be used for oil extraction, which are medicine and chemical raw materials...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 辛全伟慕德宇慕宗昭邵云华章振华董举文高畅
Owner 上海菁艺生物科技有限公司
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