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Tissue culture propagation method for helleborus thibetanus

A technology of iron chopsticks and tissue culture, applied in the field of plant breeding, can solve the problems of low value-added rate, difficulty in starting in vitro culture, difficulty in rooting and domestication, and achieve the effect of easy standardization

Active Publication Date: 2021-12-03
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

b class="d_n">[0003] In order to solve the existing iron chopsticks due to the dormancy characteristics of the seeds, the growth cycle is too long, and the in vitro culture is difficult to start, the pollution rate is high, the value-added rate is low, and the rooting and domestication are difficult. The invention provides a method for tissue culture and propagation of iron chopsticks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for tissue culture propagation of iron chopsticks, comprising the following steps:

[0019] (1) Selection and sterilization of explants: in autumn and August, cut out the small axillary buds whose leaves have not yet unfolded, soak them in washing powder water with a mass concentration of 15% for 6 minutes, rinse them with running water for more than 30 minutes, and place them in ultra-clean work. On the bench, first soak it with 70% alcohol for 30 seconds, then soak it with 0.2% Fungicide for 15 minutes, and finally soak it with 0.15% mercury liter for 20 minutes, rinse it with sterile water for 3 times, filter out excess water, and get The treated small axillary buds;

[0020] (2) Adventitious bud induction culture: inoculate the small axillary buds treated in step (1) on the following adventitious bud induction medium:

[0021] MS+2iP 2mg / L+6-BA 1mg / L+NAA 0.1mg / L, agar 6.5g / L, sucrose 30g / L sucrose, pH5.8;

[0022] Under the condition that the temperature ...

Embodiment 2

[0032] A method for tissue culture propagation of iron chopsticks, comprising the following steps:

[0033] (1) Selection and sterilization of explants: in August in autumn, cut out the small axillary buds with leaves that have not yet unfolded, soak them in washing powder water with a mass concentration of 30% for 3 minutes, rinse them with running water for more than 30 minutes, and place them on a super-clean workbench First, soak in 70% alcohol for 30 seconds, then soak in 0.2% Fungicide for 15 minutes, and finally soak in 0.15% mercury chloride for 20 minutes, rinse with sterile water for 3 times, and filter out excess water. The treated small axillary buds;

[0034] (2) Adventitious bud induction culture: inoculate the small axillary buds treated in step (1) in the adventitious bud induction medium, and induce culture for 40 days at a temperature of 13°C, a light intensity of 3000 lx, and a light time of 8 h / d until the explants differentiate into adventitious buds;

...

Embodiment 3

[0043] A method for tissue culture propagation of iron chopsticks, comprising the following steps:

[0044] (1) Selection and sterilization of explants: cut out the small axillary buds with leaves that have not yet unfolded in August in autumn, soak them in washing powder water with a mass concentration of 20% for 5 minutes, rinse them with running water for more than 30 minutes, and place them on an ultra-clean workbench , first soaked in 70% alcohol for 30 seconds, then soaked in 0.2% Fungicide for 15 minutes, and finally soaked in 0.15% mercuric acid for 20 minutes, rinsed with sterile water for 3 times, filtered out excess water, and obtained The treated small axillary buds;

[0045](2) Adventitious bud induction culture: inoculate the small axillary buds treated in step (1) into the induction medium, and induce culture for 50 days at a temperature of 17°C, a light intensity of 2500 lx, and a light time of 12 h / d until Adventitious buds differentiated from explants;

[0...

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Abstract

The invention provides a tissue culture propagation method for helleborus thibetanus. The tissue culture propagation method comprises the following steps of (1), obtaining and treating an explant; (2), carrying out adventitious bud induction culture; (3), carrying out adventitious bud subculture multiplication culture; (4), rooting culture; and (5), hardening seedlings and transplanting to obtain helleborus thibetanus seedlings. According to the method, hormone and a culture environment are comprehensively adjusted, so that the proliferation multiple of the adventitious buds of the helleborus thibetanus reaches 3.5 or above, the rooting rate reaches 90% or above, and the problems that the helleborus thibetanus is low in propagation coefficient, difficult to root and long in production period are solved; and besides, by keeping the excellent characters of the excellent strains, seedlings with stable excellent characters can be produced, standardization and factory operation are easy, and the excellent seedlings with the unified standard are provided for the market.

Description

technical field [0001] The invention relates to a method for tissue culture propagation, in particular to a method for tissue culture propagation with iron chopsticks, and belongs to the technical field of plant propagation. Background technique [0002] Iron chopsticks ( Helleborus ) is a perennial plant in the family Ranunculaceae. There are about 22 species in this genus, which are mainly distributed in Europe and West Asia. Only iron chopsticks ( Helleborus thibetanus )A sort of. Iron chopsticks have low plant type, dark green leaves, and peculiar flowers and leaves. They are used as potted plants and bloom in winter or early spring. They are called "Christmas roses" and have high ornamental value. Iron chopsticks mainly reproduce by seeds, but due to the dormant characteristics of seeds and the limitation of environmental conditions, it takes 10-12 weeks for fertilized seeds to mature, and it takes 26-34 weeks for mature seeds to germinate, resulting in The producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/28A01G24/10A01G24/15A01G22/00
CPCA01H4/002A01H4/008A01G24/28A01G24/10A01G24/15A01G22/00Y02P60/40
Inventor 段青王继华王祥宁杨晓杜文文马璐琳田敏
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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