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Method for Rapid Propagation of Garlic Callus and Propagation of Improved Varieties

A technology for callus and garlic, which is applied in the field of garlic callus rapid propagation and improved variety breeding, can solve the problems of low reproduction coefficient of fine varieties, and achieve the effects of improving distribution and utilization efficiency, simple method and improving rapid propagation efficiency.

Active Publication Date: 2021-04-06
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a method for rapid propagation of garlic callus and breeding of improved varieties, so as to solve the problem of low reproductive coefficient of fine varieties in traditional garlic breeding methods

Method used

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  • Method for Rapid Propagation of Garlic Callus and Propagation of Improved Varieties
  • Method for Rapid Propagation of Garlic Callus and Propagation of Improved Varieties
  • Method for Rapid Propagation of Garlic Callus and Propagation of Improved Varieties

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Embodiment Construction

[0047] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0048] The concrete steps of garlic callus rapid propagation and improved seed breeding method are as follows:

[0049] Step S1, selecting materials;

[0050] The nutritional status of the garlic harvested from October to June of the following year is better, and this is the best time for the first generation of garlic to be cultured indoors for detoxification and rapid propagation. Choose plump garlic scale buds without disease, number and record each sample...

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Abstract

The invention discloses a method for rapid propagation of garlic callus and breeding of improved varieties. The specific steps are as follows: step S1, select full and disease-free garlic scale buds harvested from October of the current year to June of the second year, and remove the outer leaves; step S2, taking the bulb disc, outer leaf sheath, inner leaf sheath and root tip of garlic bulbs respectively as explant-induced callus; step S3, after 45-50 days of explant-induced callus, subculture the callus Propagation and differentiation to obtain regenerated plants; Step S4, inducing miniature garlic or field transplanting on the regenerated plants to obtain the original species; Step S5, sowing the original species to breed the original garlic, and multiplying the original garlic. The callus induction medium for explant-induced callus is B5+NAA 0.5mg / l+6-BA 0.1mg / l+2,4-D 1.0mg / l+sucrose 30g / L medium. The problem of low reproductive coefficient of fine garlic varieties is solved.

Description

technical field [0001] The invention belongs to the field of agricultural product planting, in particular to a method for quick propagation of garlic callus and breeding of improved varieties. Background technique [0002] Garlic (Allium sativum L.) is a plant of the genus Allium (Allium spp.) of the Liliaceae (Liliaceae). Garlic is a typical asexually propagated crop, and bulbs are usually used for reproduction in production. Once the virus invades the garlic plant, because the bulb mother carries the virus, It is passed on to offspring in the form of vertical infection, resulting in an increase in the concentration of the virus in the garlic from generation to generation, causing the degradation of the species, and the decrease in yield and quality year by year. Plant shoot tip culture is an important way to solve the above problems, but excellent garlic varieties need to quickly meet the needs of production, and only through field bulb or shoot tip culture still cannot me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王海平李锡香武亚红宋江萍张晓辉邱杨
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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