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Tissue-culture seedling raising method of rhodiola crenulata

A technology of Rhodiola grandiflora and single seedlings, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve problems such as inability to carry out large-scale planting and production, long subculture and multiplication cycle, and inability to realize large-scale production, and achieve a solution Difficult rooting, early start, and improved germination rate

Inactive Publication Date: 2012-04-25
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, people solve the demand problem through various ways such as introduction and cultivation, but with little effect
[0004] Adopting tissue culture method is to explore the main way of fast asexual reproduction of Rhodiola grandiflora, and there is no report about the tissue culture regeneration of Rhodiola grandiflora abroad, and some research work on tissue culture of Rhodiola grandiflora has been done in China, such as Yin Wenbing et al. "Tissue Culture and Rapid Propagation of Rhodiola grandiflora", Yin Wenbing et al., Plant Physiology Communications, Volume 41, No. 4, Page 493, 2005) by inducing leaves to produce callus, producing young shoots, and then inducing young Shoots take root to form rooted shoots, but different culture media and hormones must be used in different stages of this method, which is time-consuming and labor-intensive, and the possibility of callus variation is also greater
Due to the browning of the rooting process, the treatment method is to continuously transfer, so that it cannot be scaled; Yan Yingcai et al. Volume 41, No. 3, page 341, 2005) has also done research on the regeneration of Rhodiola grandis, and its subculture medium is 6-benzylaminoadenine (6-BA)+indole acetic acid (IAA) , but its subculture multiplication cycle is long, the multiplication coefficient is low, and the adventitious buds multiplied by 3 times in 40 days
It can be seen that the current tissue culture of Rhodiola grandiflora still has the disadvantages of slow initiation of new shoots and low reproduction coefficient, and cannot be used for large-scale planting and production.

Method used

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  • Tissue-culture seedling raising method of rhodiola crenulata

Examples

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Effect test

Embodiment 1

[0041] The concrete steps of the group cultivation seedling method of Rhodiola grandiflora in the present embodiment are as follows:

[0042] (1) Select the leaves of Rhodiola grandiflora excellent single plant (collected from perennial wild plants in Tibet) as explants. When disinfecting the explants, wash them with tap water for 30 minutes, and then wash them in a sterile ultra-clean bench. Disinfect with 10% 84 disinfectant for 25 minutes, and then wash with sterile water for 3 times.

[0043] (2) Sterilized explants were induced to sprout under sterile conditions, the basic medium was MS medium, and the additional plant hormones were 15 μmol / L of 6-BA and 2.5 μmol / L of GA 3 , also contains 5.5g / L of agar and 26g / L of sucrose, and the pH of the medium is 5.8. The explants were treated in the dark for 10 days on the germination induction medium, and then cultured under the condition of light intensity of 2500±500Lux, the culture temperature of the induced adventitious buds wa...

Embodiment 2

[0048] The concrete steps of the group cultivation seedling method of Rhodiola grandiflora in the present embodiment are as follows:

[0049] (1) Select the leaves of Rhodiola daflora excellent individual plants as explants. When disinfecting the explants, wash them with tap water for 30 minutes, and then disinfect them with 10% 84 disinfectant in a sterile ultra-clean bench. 25 minutes, and then washed 3 times with sterile water.

[0050] (2) Sterilized explants were induced to sprout under sterile conditions, the basic medium was MS medium, and the additional plant hormones were 15 μmol / L of 6-BA and 2.5 μmol / L of GA 3 , also contains 6g / L of agar and 20g / L of sucrose. The explants were treated in the dark on the germination induction medium for 5 days, and then cultivated under the condition of light intensity of 2500±500Lux, the culture temperature of induced adventitious buds was 25±2°C, the pH of the medium was 5.8, and the light time was 16 hours, 12 Days differentiat...

Embodiment 3

[0055] The concrete steps of the group cultivation seedling method of Rhodiola grandiflora in the present embodiment are as follows:

[0056] (1) Select the leaves of Rhodiola daflora excellent individual plants as explants. When disinfecting the explants, wash them with tap water for 30 minutes, and then disinfect them with 10% 84 disinfectant in a sterile ultra-clean bench. 25 minutes, and then washed 3 times with sterile water.

[0057] (2) Sterilized explants were induced to sprout under sterile conditions, the basic medium was MS medium, and the additional plant hormones were 15 μmol / L of 6-BA and 2.5 μmol / L of GA 3 , also contains 6.5g / L of agar and 30g / L of sucrose. The explants were treated in the dark for 0 days on the germination induction medium, and then cultured under the condition of light intensity of 2500±500Lux, the culture temperature of induced adventitious buds was 25±2°C, the pH of the medium was 5.8, and the light time was 16 hours, Days differentiated....

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Abstract

The invention provides a tissue-culture seedling raising method of rhodiola crenulata, which comprises the following steps of: (1) taking the leaves of the rhodiola crenulata as an explant and carrying out budding culture to form adventitious buds; (2) carrying out propagation culture on the adventitious buds to form single seedlings; (3) carrying out rootage culture on the single seedlings obtained after stretching cluster buds to form rootage seedlings; and (4) transplanting the rootage seedlings, wherein a culture medium for the budding culture is an MS culture medium added with 10 to 20mumol / L of 6-benzylaminopurine and 1 to 5mumol / L of gibberellin. The invention reinforces the high-efficiency seedling raising method of the rhodiola crenulata by primary culture dark processing and thecombined processing of the addition of active carbon in the rootage process and solves the problem of low propagation coefficient of the rootage seedlings of the rhodiola crenulata, the multiplication coefficient of 20 days of the cluster buds is 2 to 3 times, the rootage rate reaches more than 95 percent, and the transplanting survival rate is as high as more than 90 percent. The method providedby the invention has high differentiation frequency and short growth cycle and is easy for the large-scale production of the rhodiola crenulata.

Description

technical field [0001] The invention relates to a method for group seedling cultivation of plants, in particular to a method for group seedling cultivation of Rhodiola grandiflora. Background technique [0002] Rhodiola crenulata (Rhodiola crenulata) is a perennial herb of the genus Rhodiola (Rhodiola L.) of Crassulaceae (Crassulaceae). The impact of harsh environments such as strong ultraviolet rays has formed a unique nutritional composition, which is rich in glycosides, flavonoids, multivitamins and trace elements, and also contains 17 kinds of amino acids necessary for the body. It is known as "plateau ginseng" and is the national It is the only Rhodiola medicinal material included in the 2005 Pharmacopoeia. Studies have shown that Rhodiola daflora has multiple functions such as anti-hypoxia, anti-fatigue, anti-radiation, and delaying the aging of the body. [0003] Rhodiola grandiflora is mostly natural resources, its living environment is harsh, its growth is slow an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 刘春朝赵燕
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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