262 results about "6-Benzylaminopurine" patented technology

The invention discloses a plant growth regulating composition and its application. The composition comprises the following components: A) natural abscisic acid, B) gibberellic acid (GA3, or of GA4 +7), and C) an existing plant growth regulator. The existing plant growth regulator is selected from one or more than two of 6-BA (6-benzylaminopurine), thidiazuron, brassinolide, sodium nitrophenolate, potassium nitrophenolate, chlormequat chloride, triacontanol, naphthlcetic acid and its salt, indolebutyric acid and its salt, indoleacetic acid and its salt, ethephon, diethyl aminoethyl hexanoate, paclobutrazol, uniconazole, fulvic acid, Mepiquat chloride, nitrohumic acid, jasmonic acid and triapenthenol. Specifically, the components A), B) and C) are in a weight ratio of 1:0.001-500:0.001-500. The S-ABA (S-abscisic acid) added in the invention can expand the prevention and treatment objects, reduce or prevent phytotoxicity, reduce dosage, and lower the use cost.

Personal care composition comprising from about 0.05% to about 5% of at least one aquaporin-stimulating compound selected from the group consisting of xanthine, caffeine; 2-amino-6-methyl-mercaptopurine; 1-methyl xanthine; 2-aminopurine; theophylline; theobromine; adenine; adenosine; kinetin; p-chlorophenoxyacetic acid; 2,4-dichlorophenoxyacetic acid; indole-3-butyric acid; indole-3-acetic acid methyl ester; beta-naphthoxyacetic acid; 2,3,5-triiodobenzoic acid; adenine hemisulfate; n-benzyl-9-(2-tetrahydropyranyl)adenine; 1,3-diphenylurea; 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea; zeatin; indole-3-acetic acid; 6-benzylaminopurine; alpha-napthaleneacetic acid; 6-2-furoylaminopurine; green tea extract; white tea extract; menthol; tea tree oil; ginsenoside-RB1; ginsenoside-RB3; ginsenoside-RC; ginsenoside-RD; ginsenoside-RE; ginsenoside-RG1; ginseng root extract; ginseng flower extract; pomegranate extract, extracts from Ajuga turkestanica; extracts from viola tricolor and combinations thereof; an additional ingredient selected from the group consisting of niacinamide, glycerin and mixtures thereof, and a dermatologically-acceptable carrier.

The invention discloses a fresh flower preservative, which comprises the following components in percentage by weight: 0.5 to 4.0 percent of sucrose, 0.01 to 0.05 percent of 8-hydroxyquinoline sulfate, 0.001 to 0.004 percent of 1-methyl cyclopropene, 0.0005 to 0.002 percent of gibberellin, 0.01 to 0.06 percent of calcium nitrate, 0.05 to 0.3 percent of garlic extract, 0.05 to 0.2 percent of oliveleaf extract, 0.03 to 0.15 percent of citric acid, 0.001 to 0.004 percent of 6-benzylaminopurine, and the balance of water. The invention also discloses a preparation method of the fresh flower preservative. The novel biological preservative (carnosine) is blended with the traditional additive, so that the defects of the conventional fresh flower preservative are overcome; the degradability advantage of the biological preservative is used; the fresh flower preservative is a product which is convenient to use and has good fresh keeping effect and no toxic hazard effect; and the fresh flower preservative meets the requirements of customers on spiritual civilization, and a technical scheme and a product are provided for solving the problem of difficulty in keeping flowers fresh.

The invention discloses a root-promoting liquid fertilizer, which is prepared from the following raw materials in parts by weight: 2-4 parts of ferrous sulfate, 2-3 parts of ammonium molybdate tetrahydrate, 1-3 parts of potassium pyrophosphate, 5-7 parts of short rhizome of spathiphyllum, 6-9 parts of castor cake, 15-20 parts of bran, 5-8 parts of potassium sulfate, 7-10 parts of urea, 2-4 parts of matrine, 2-3 parts of zinc sulphate heptahydrate, 0.1-0.2 part of 6-benzylaminopurine, 0.1-0.2 part of triiodobenzoic acid, 4-8 parts of rose, 10-14 parts of brewer's grains and 8-12 parts of an ingredient. Due to the addition of the 6-benzylaminopurine, the triiodobenzoic acid and the like, the liquid fertilizer disclosed by the invention has the function of adjusting the raw materials with a crop growth function, and is capable of effectively promoting rooting and formation of flower buds of the crops; the nutrient components of a microelement fertilizer can be increased by extract liquids such as chaff and brewer's grains; and the root-promoting liquid fertilizer is convenient to use, and obvious in yield-increasing effect, and has a good application prospect.

The invention relates to a tender stem callus plant regeneration induction culture medium for lonicera caerulea and solves the problems of low plant differentiation rate and high culture medium cost problem of the auxiliary bud or stem tip culture, which is the main tissue culture of lonicera caerulea. The culture medium consists of a large amount of macroelements, microelements and hormone, wherein the hormone consists of 6-benzyl aminopurine at a concentration of 1.0 to 2.0mg/L and indolebutyric acid or naphthylacetic acid at a concentration of 0.05 to 0.2mg. When the LD1 culture medium of the invention is used, the tender stems of lonicera caerulea can be easily induced to form calla, and the induction rates of the calla are all over 90 percent; and the probability of the induction of the calla into plants is high, the plant differentiation rate is up to 50 to 82 percent which is 66.6 to 173.3 percent higher than that of the conventional Murashige-Skoog (MS) culture medium, and the cost is saved by 45.1 percent compared with the reference MS culture medium.

The invention provides a cultivation method for beak-shaped litchis. The cultivation method for the beak-shaped litchis comprises the step of harvesting, wherein clusters and 2-3 leaves at the lower section of each cluster are picked; the step of autumn growth promotion, wherein additional nitrogen phosphorus and potassium fertilizer is applied; the step of root irrigation through polyethylene glycol with concentration of 20%; the step of control over winter growth and ringing, wherein ethyl alcohol with concentration of 95% is sprayed to the ringing position, and whitewash with concentration of 2-4% is sprayed to the leaves; the step of flower bud promotion, wherein mixed cytokinin is sprayed one to two times during the germination period; the step of flower thinning and bud thinning, wherein the mixed solution of ethephon and paclobutrazol is sprayed for bud thinning during the squaring period, and the mixed solution of calcium phosphate, glycine and saccharose fatty acid is sprayed for flowing mining during the flower period; the step of stabilizing of fruits, wherein the mixed solution of monopotassium phosphate, 6-benzylaminopurine, naphthylacetic acid, ethylene glycol, cane sugars, borax and ethyl alcohol with concentration of 95% is sprayed during the growth period of the fruits. The cultivation method for the beak-shaped litchis is designed according to the characteristics of the beak-shaped litchis, is simple in step and convenient to operate, saves manpower and material resources, and effectively improves the seedless rate of the fruits, the quality of the fruits of the beak-shaped litchis, and the yield.

The invention discloses honeysuckle rooting liquid. Each liter of solution comprises the following ingredients in parts by weight: 3 to 6 parts of ABT rooting powder, 20 to 30 parts of heteroauxin, 20 to 30 parts of naphthylacetic acid, 2 to 3 parts of potassium nitrate, 1 to 2 parts of copper sulfate, 2 to 4 parts of zinc sulfate, 5 to 10 parts of polyving akohol, 10 to 20 parts of ammonium nitrate, 3 to 5 parts of vitamin D, 5 to 8 parts of 4-iodiphenoxyaceticacid, 20 to 30 parts of 6-benzylaminopurine and 20 to 30 parts of gibberellin. The honeysuckle rooting liquid has the advantages that the cutting seedling rooting of honeysuckles can be promoted, the rooting time is shortened, the rooting rate is improved, the honeysuckle rooting liquid also contain trace elements, the vegetative growth can be accelerated, deep roots and luxuriant leaves are realized, and crop individuals develop strongly, so the survival rate of the cutting seedlings of the honeysuckles can be improved.

The invention discloses plant infusion nutrient solution and a preparation method thereof and aims to provide nutrient solution which can be directly infused into the plants, stimulate the tree activity, promote the plants to rapidly take root and sprout, quickly supply nutrition to the trees, and enhance the tree stress resistance, and a preparation method of the nutrient solution. The proportion of the nutrient solution is that: every 1,000ml of deionized water contains 0.05 to 0.1g of monopotassium phosphate, 0.02 to 0.045g of potassium nitrate, 0.15 to 0.3g of calcium nitrate tetrahydrate, 0.11 to 0.2g of magnesium sulfate heptahydrate, 0.03 to 0.06g of ferrous sulphate heptahydrate, 0.07 to 0.15g of disodium ethylene diamine tetraacetate, 0.015 to 0.025g of manganese sulfate tetrahydrate, 0.012 to 0.018g of zinc sulfate heptahydrate, 0.04 to 0.06g of copper sulfate pentahydrate, 0.015 to 0.022g of ammonium molybdate, 0.015 to 0.02g of indolebutyric acid, and 0.008 to 0.015g of 6-benzylaminopurine. The plant infusion nutrient solution satisfies the needed nutrient in the process of transplanting big trees, has high absorption utilization ratio, quick response, stable form, high activity, and is easily absorbed by the plants.

The invention discloses a method for producing a Fujian jewel orchid by plant tissue culture, which comprises the following three procedures: selection and sterile treatment of plants, induced culture of cluster buds and large-scale culture. The method is technically characterized in that two different culture media are used in the induced culture procedure and the large-scale culture procedure, wherein the culture medium for the induced culture comprises an MS culture medium, sucrose of 10-50g/L, agar of 6-8g/L, activated carbon of 2.0-3.0g/L, naphthylacetic acid of 0.1-3.0mg/L and 6-benzylaminopurine of 0.1-5.0mg/L, and the pH value is 5.0-7.0; and the culture medium for the large-scale culture comprises a modified MS culture medium, calcium nitrate of 600mg/L, mashed banana of 30-50g/L, sucrose of 20-40g/L, agar of 6-8g/L, activated carbon of 2.0-3.0g/L, naphthylacetic acid of 1.0-2.0mg/L and 6-benzylaminopurine of 2.0-3.0mg/L, and the pH value is 5.0-7.0. Plant tissue culture can keep the good characters of the plants. The method has the advantages of easy operation, low production cost, high yield, good quality and no environment pollution, is suitable for industrial seedling raising and can realize mass production.

The invention relates to a method for regenerating plant fromarundo donax linn callus. The method comprises the following steps of (1) inducing callus: inoculating arundo donax linn tissue into culture medium containing1.0-5.0mg/l of 2,4-D (2,4-dichlorophenoxyacetic acid), carrying out dark cultivation for 3 days after inoculating, and then cultivating under the condition that the light period is 16 hours of illumination and 8 hours of darkness, and the illumination intensity is 2000Lx; (2) succesive transfer culture of callus: inoculating the callus into the culture medium containing 5.0mg/l of 2,4-D; (3) regenerating the callus plant: taking MS culture medium as a basic culture medium, and adding 0.5mg/L of IBA (indolebutyric acid) and 5.0mg/L of 6-BA (6-benzylaminopurine); (4) sprouting cultivation; (5) rooting cultivation: inoculating the plant into the MS culture medium in which 0-0.5mg/L of NAA (naphthylacetic acid) is added to root after sprouting; and (6) transplanting. A method for regenerating the plant from arundo donax linn callus is built by the invention; and expanding propagation of excellent arundo donax linn is facilitated.

The invention consists of an aquatic plant product for resale, having an extended shelf life, and a method for making the aquatic plant product. The aquatic plant product is made of a sealed container containing aquatic-plant-growth-sustaining medium therein and an aquatic plant planted in that aquatic-plant-growth-sustaining medium. The aquatic-plant-growth-sustaining medium contains a root-support material; at least one vitamin; sucrose; agar; distilled water; micro-nutrients containing iron; at least one hormone selected from a group consisting of auxin, naphthleneacetic acid, indole-3-butric acid, cytokine, 2-isopentyladenine, 6 Benzylaminopurine and any combination thereof.

The present invention uses improved MS culture medium as basis, and adds the active carbon, indole-3-acetic acid, 3-indolebutyric acid and 6-benzylaminopurine as auxiliary agent to make tissue cultivation of two key stages of induced growth of bunch bud and seedling-strengthening and rooting of camllia chrysantha. The invention can be used for implementing industrial quick seedling cultivation.

The present invention relates to a high-efficient breeding technique of Eurasian raisin grape. After the raisin grape has flowered for 30-50d, the young fruit of the grape is picked. The ovule is taken out in a sterilized state. The embryo is inoculated to an MS culture medium containing different levels of plant growth regulators of gibberellic acid (GA3), indoleacetic acid (IAA) and 6-benzylaminopurine (6-BA) for executing embryo rescue. The average embryo development rate using the breeding technique of the invention is increased from 8.3-11.9% of a patient disclosed in the background art to 84%. The germination rate obtains 75.33% and the seedling rate obtains 74.17%. Furthermore the embryo rescue technique of the raisin grape obtained through the technique of the invention has the advantages of increased seedling rate of offspring, easier obtainment of more filial generation, increased parent selection range of raisin grape cross breeding, accelerated breeding process of new species of the raisin grape and increased breeding efficiency of the raisin grape through increasing the embryo development rate and the germination rate of the raisin grape.

The invention discloses a method for breeding locusts, which uses zygotic embryo of different embryonic ages as explants, and uses MS+2, 4-D+BA+cane sugar+agar as an induction medium of an embryonic callus. The method comprises: using MS basal medium + MES + glutamine + casein hydrolysate + naphthylacetic acid + 6-Benzylaminopurine+cane sugar+agar as a somatic cell embryo medium, by inducting thecallus, transferring the formed globular embryo onto MS+casein hydrolysate medium, carrying out maturation culture of somatic embryo, and bourgeoning the cultured somatic embryo on a basal medium to from a complete small plant. The method of the invention has the advantages of high callus inductivity, high somatic embryo inductivity and high germination rate, and can cultivate a large numbers of locust test-tube plantlets in a short period for large scale and factory production.

The invention discloses a culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes, which belongs to the field of agricultural biotechnologies. The culture medium comprises the following compositions: potassium nitrate, ammonium nitrate, monopotassium phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulfate, cobalt chloride, ferrous sulfate, disodium ethylenediamine tetraacetic acid, nicotinic acid, pyridoxine hydrochloride, glycine, glutamine, casein hydrolysate, yeast extracts, sucrose, 6-benzylaminopurine, naphthalene acetic acid, kinetin, zeatin and heteroauxin beilining. The culture medium is applied to the induction and culturing of double-haploid stem regenerated seedlings of potatoes, the test effect is good, the initiating rate is increased by 76%, and the seedling rate is as high as 80.5%. The regeneration rate of double-haploid stems of potatoes is significantly increased, thereby laying a foundation for the establishment of a genetic transformation system; and the culturing mode adopts a one-step seedling method, so that the pollution probability is reduced.

The invention provides a tissue-culture seedling raising method of rhodiola crenulata, which comprises the following steps of: (1) taking the leaves of the rhodiola crenulata as an explant and carrying out budding culture to form adventitious buds; (2) carrying out propagation culture on the adventitious buds to form single seedlings; (3) carrying out rootage culture on the single seedlings obtained after stretching cluster buds to form rootage seedlings; and (4) transplanting the rootage seedlings, wherein a culture medium for the budding culture is an MS culture medium added with 10 to 20mumol/L of 6-benzylaminopurine and 1 to 5mumol/L of gibberellin. The invention reinforces the high-efficiency seedling raising method of the rhodiola crenulata by primary culture dark processing and the combined processing of the addition of active carbon in the rootage process and solves the problem of low propagation coefficient of the rootage seedlings of the rhodiola crenulata, the multiplication coefficient of 20 days of the cluster buds is 2 to 3 times, the rootage rate reaches more than 95 percent, and the transplanting survival rate is as high as more than 90 percent. The method provided by the invention has high differentiation frequency and short growth cycle and is easy for the large-scale production of the rhodiola crenulata.

The invention discloses a cherry rootstock tissue culture medium, which is prepared from 2 to 60 ml/L of optimized MS base mother solution, 2545 g/L of sucrose, 6.5 to 7.0 g/L of agar, 0.4 to 0.6 mg/L of 6-Benzylaminopurine, 0.1 to 0.3 mg/L of indolebutyric acid, 0.061 to 0.067 mg/L of VC, 0.05 to1.00 mg/L of indoleacetic acid and 1000 to1200 mg/L of active carbon; the culture medium is specifically applied to the growth and cultivation of the bud of the dwarf cherry rootstock, the cultivation of the strong stock and the cultivation of the induced root; the proliferation and root-growing effect is excellent so that the root-growing rate of the dwarf cherry rootstock can reach 95% to 98%; the root is rough and tidy; the culture bud is healthy; the leaf is green, therefore, the labor and material are effectively saved; the survival rate of the transplant can reach 95%, and the foundation is established for the large-scale production. The tissue culture medium method disclosed by the invention is simple and easy, thereby simplifying the program, improving the efficiency, and reducing the cost. In the invention, the problems of brown stain and vitrification in the culture process of tissue culture medium can be solved; and therefore, the invention can be applied to the fast reproduction of the Gisela sweet dwarf cherry rootstock in the large-scale production.

The invention discloses a propagation method for submerged plant potamogeton pectinatus L, comprising the following steps: culturing the potamogeton pectinatus L in the Hoagland culture solution; inducing roots to grow when adding 0.5-2mg/L of indolebutyric acid; and inducing lateral buds to grow when adding 0.5-2mg/L of 6-benzylaminopurine. After being cultured for 15-20 days indoors, the potamogeton pectinatus L is transplanted to a seedling field to be cultured for 30-35 days; after the plant grows to 20-30cm, the potamogeton pectinatus L is transplanted to the target water. With simple operation and high survival rate, the propagation technology for potamogeton pectinatus L solves the problem that the explants of the potamogeton pectinatus L cannot root when being propagated.

The invention discloses a bacterial bio-fertilizer synergist and a production method thereof. The synergist has the following components: 10-20% of bacillus subtilis, 15-30% of polyaspartic acid, 5-10% of alpha-naphthyl acetic acid (sodium), 10-20% of 2, 4-dinitrophenol (sodium), 5-10% of sodium para-nitrophenolate and 5-10% of 6-benzylaminopurine. The production method is as follows: the 10-20% of bacillus subtilis, the 15-30% of polyaspartic acid, the 5-10% of alpha-naphthylacetic acid (sodium), the 10-20% of 2, 4-dinitrophenol (sodium), the 5-10% of sodium para-nitrophenolate and the 5-10% of 6-benzylaminopurine are added to a stirrer for thoroughly and evenly mixing, bagged in a plastic bag and sealed, thus the product is obtained. When in use, the bag of the biological bacteria fertilizer synergist is unpacked; the synergist is mixed with the applied chemical fertilizer according to the amount of 10-20g per mu, and then applied according to the application method of the chemical fertilizer.

The invention discloses an ex-vitro soilless cutting rooting method for tissue-cultured and proliferated seedlings of gerbera jamesonii bolus. The method mainly comprises the following steps of: transferring induction-cultured buds to a proliferation culture medium to culture, wherein the proliferation culture medium is MS (Murashige and Skoog) basic culture solution containing 0.3-0.7 mg/L of 6-BA (6-benzylaminopurine), 0.1 mg/L of NAA (naphthylacetic acid), 30000 mg/L of cane sugar, and 6500 mg/L of agar; when the proliferated seedlings are grown, moving a proliferation culture bottle in a greenhouse covered by a sunshade net having a light-shading rate of 70% to perform seedling hardening for 4-7 days; and then performing ex-vitro soilless cutting rooting culture on the proliferated seedlings. According to the invention, a rooting phase is soilless rooting culture in the greenhouse, thus enhancing the environmental adaptability of the rooted seedlings, ensuring the rooting rate of the seedlings, increasing the survival rate of cutting transplantation, keeping the technical advantages of tissue culture, overcoming the problems of low seedling-hardening survival rate, high production cost, many production links and the like of the tissue-cultured seedlings, and reducing soil-borne diseases at seedling stage; therefore, high-quality seedlings can be provided for soilless culture for gerbera jamesonii bolus.

The invention discloses a method for rapidly breeding potted carnation through tissue culture. The method comprises the following steps of: cutting an explant and sterilizing; carrying out primary culture, wherein a primary culture medium comprises MS (Murashige and Skoog), 1.5mg/L of 6-BA (6-Benzylaminopurine), 0.2mg/L of NAA (Naphthalene Acetic Acid), 30g/L of sucrose and 8g/L of agar; carrying out subculture, wherein a subculture medium comprises MS, 0.8mg/L of 6-BA, 0.2mg/L of NAA, 30g/L of sucrose and 8g/L of agar; carrying out rooting culture, wherein a rooting culture medium comprises MS, 0.2mg/L of NAA, 0.1mg/L of IAA(Indole Acetic Acid), 30g/L of sucrose and 8g/L of agar; and washing the culture mediums away and then holding the temperature and humidity for seedling hardening and transplanting. The method is simple and easy to operate, simple in procedure and high in working efficiency, and is capable of effectively holding excellent properties of parents. According to the method, the differentiation rate of a tissue culture seedling is increased. The method has the characteristics of high multiplication speed, high rooting rate, high survival rate and low cost.

The invention provides a method for overcoming yellowing of leaves of tissue culture seedlings of Rosa damascena. The method comprises: taking rosa damascena stem segments with axillary buds as explants to be sterilized, inoculating the sterilized stem segments into an MS (Murashige and Skoog) culture medium to be subject to primary culture; and cutting the seedlings obtained after primary culture into segments and transferring the seedling segments to a multiplication culture medium to be subject to subculture at intervals, wherein 30g of cane sugar and 6g of agar are added to each litre of the MS culture medium; each seedling segment is provided with a leaf; the multiplication culture medium is the MS culture medium to each litre of which 0.5-4.0mg of 6-BA (6-benzylaminopurine), 0.5-2.0mg of KT (kinetin), 0.2mg of NAA (1-Naphthaleneacetic acid), 5-20mg of ABA (abscisic acid), 1-10mg of AgNO3, 30g of cane sugar and 6g of agar are added; and the primary culture and the subculture are carried out under the following conditions: the illumination intensity is 1500-2000Lx, the temperature is 23+/-2 DEG C, and the humidity is 75-80%.

The invention discloses a tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts. The medium comprises at least one of the following mediums: a callus induction medium which comprises B5, 2mg/L of 6-BA (6-benzylaminopurine), 2mg/L of KT (kinetin), 0.1mg/L of NAA (naphthylacetic acid), 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; an adventitious bud differentiation medium which comprises B5, 1mg/L of 6-BA, 2mg/L of KT, 0.1mg/L of NAA, 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; an adventitious bud subculture medium which comprises MB, 1mg/L of 6-BA, 0.8mg/L of IAA (indoleacetic acid), 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; and a rooting medium which comprises 1/3MB, 1.5mg/L of IBA (indolebutyric acid), 0.1mg/L of NAA, 5.5-6.0g/L of carragheenan and 20g/L of sucrose and has a pH value of 5.6-5.8. The medium of the invention, which has the advantages of high callusinductivity, high dventitious bud differentiation rate and propagation coefficient, high rooting rate, high transplanting survival rate of test tube sprouts, robust sprout growth, normal leaf shape and leaf color, rapid subcutaneous rooting and new leaf germination, and the like, can satisfy needs of the large-scale cultivation of Lonicera macranthoides Hand. Mazz Yulei No.1, and has a good application prospect.

The invention relates to a seed soaking agent, in particular to a multifunctional high-efficiency seed soaking agent for sugarcanes. The multifunctional high-efficiency seed soaking agent for the sugarcanes comprises the following ingredients: 6-benzylaminopurine, indole potassium butyrate, thiophanate methyl, trichlorfon and high-efficiency chelating agent. The multifunctional high-efficiency seed soaking agent for sugarcanes, provided by the invention, is a safe and high-efficiency sugarcane seed soaking agent capable of preventing diseases, killing insects and increasing sprouting percentage and germination rate, which is developed according to the latest scientific research achievements of the agricultural chemical aspects as well as academic principles like phytopathology, agricultural entomology, phytophysiology and the like.