The invention discloses a tissue medium for
Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts. The medium comprises at least one of the following mediums: a
callus induction medium which comprises B5, 2mg / L of 6-BA (6-benzylaminopurine), 2mg / L of KT (
kinetin), 0.1mg / L of NAA (
naphthylacetic acid), 5.5-6.0g / L of carragheenan and 30g / L of
sucrose and has a pH value of 5.6-5.8; an adventitious
bud differentiation medium which comprises B5, 1mg / L of 6-BA, 2mg / L of KT, 0.1mg / L of NAA, 5.5-6.0g / L of carragheenan and 30g / L of
sucrose and has a pH value of 5.6-5.8; an adventitious
bud subculture medium which comprises MB, 1mg / L of 6-BA, 0.8mg / L of IAA (indoleacetic acid), 5.5-6.0g / L of carragheenan and 30g / L of
sucrose and has a pH value of 5.6-5.8; and a rooting medium which comprises 1 / 3MB, 1.5mg / L of IBA (
indolebutyric acid), 0.1mg / L of NAA, 5.5-6.0g / L of carragheenan and 20g / L of sucrose and has a pH value of 5.6-5.8. The medium of the invention, which has the advantages of high callusinductivity, high dventitious
bud differentiation rate and propagation coefficient, high rooting rate, high
transplanting survival rate of
test tube sprouts, robust sprout growth, normal leaf shape and leaf color, rapid subcutaneous rooting and new leaf
germination, and the like, can satisfy needs of the large-scale cultivation of
Lonicera macranthoides Hand. Mazz Yulei No.1, and has a good application prospect.