Ex-vitro soilless cutting rooting method for tissue-cultured and proliferated seedlings of gerbera jamesonii bolus

A cutting and tissue culture technology, applied in the field of plant biotechnology and cultivation, can solve the problems of restricting the wide application of soilless culture technology, long time for slow seedling transition culture, weak adaptability of rooting seedlings, etc. Effects of soil-borne diseases in seedling stage and guaranteeing rooting rate

Active Publication Date: 2012-09-12
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is mainly to carry out rooting culture in the culture medium in the bottle of culture room condition for prior art gerbera, cause the rooting seedling adaptability of cultivating to be weaker, slow seedling transition culture time is longer, transplanting The survival rate is low; the seedlings carry serious soil-borne diseases, which restrict the defects and deficiencies in the widespread application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Selection and disinfection of explants: Select a receptacle with a size of 1 to 2 cm from the gerbera plant; rinse the receptacle under tap water for 5 minutes, then wash it with washing powder water with a mass concentration of 0.1% for 5 minutes, rinse it and put it on In a sterile ultra-clean bench, put the receptacle into an ethanol solution with a mass concentration of 70% and soak it for 10 seconds, rinse it twice with sterile water, soak it in mercuric chloride with a mass concentration of 0.1% for 10-15 minutes, and then soak it in 100ml of mercuric acid with a mass concentration of 0.1%. Soak in a solution of 0.2% sodium hypochlorite plus two drops of Tween 20 for 10 minutes, and finally rinse with sterile water for 4 times, and shake the vessel fully during the soaking process;

[0026] 2. Induction culture: cut off the pedicel and calyx from the receptacle that has been sterilized in step 1, and cut it into a size of 0.5-1 cm, and put it into a culture bott...

Embodiment 2

[0046] Step 1 explant selection and sterilization are identical with embodiment 1 step 1;

[0047] Step 2 induction culture is identical with embodiment 1 step 2;

[0048] Step 3, except that the following operations are different, all the other operating methods are the same as Step 3 of Example 1:

[0049] The 6-benzylaminopurine (6-BA) in the proliferation medium is 0.7mg / L, P H is 6.0

[0050] Proliferation effect: the multiple of each subculture is up to 4 times

[0051] Step 4 seedling hardening, except that following operation is different, all the other operation methods are identical with embodiment 1 step 4:

[0052] The hardening time is 7 days, and the temperature of the transplanting greenhouse is controlled at 23°C to 28°C;

[0053] Step 5 takes root without soil cuttings outside the bottle, except that the following operations are different, and all the other operating methods are the same as in Example 1 step 5:

[0054] Rooting water is an aqueous...

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Abstract

The invention discloses an ex-vitro soilless cutting rooting method for tissue-cultured and proliferated seedlings of gerbera jamesonii bolus. The method mainly comprises the following steps of: transferring induction-cultured buds to a proliferation culture medium to culture, wherein the proliferation culture medium is MS (Murashige and Skoog) basic culture solution containing 0.3-0.7 mg/L of 6-BA (6-benzylaminopurine), 0.1 mg/L of NAA (naphthylacetic acid), 30000 mg/L of cane sugar, and 6500 mg/L of agar; when the proliferated seedlings are grown, moving a proliferation culture bottle in a greenhouse covered by a sunshade net having a light-shading rate of 70% to perform seedling hardening for 4-7 days; and then performing ex-vitro soilless cutting rooting culture on the proliferated seedlings. According to the invention, a rooting phase is soilless rooting culture in the greenhouse, thus enhancing the environmental adaptability of the rooted seedlings, ensuring the rooting rate of the seedlings, increasing the survival rate of cutting transplantation, keeping the technical advantages of tissue culture, overcoming the problems of low seedling-hardening survival rate, high production cost, many production links and the like of the tissue-cultured seedlings, and reducing soil-borne diseases at seedling stage; therefore, high-quality seedlings can be provided for soilless culture for gerbera jamesonii bolus.

Description

technical field [0001] The invention relates to a soilless cutting rooting method for gerbera tissue culture multiplication seedlings outside a bottle, and belongs to the field of plant biotechnology and cultivation technology. Background technique [0002] Gerbera ( Gerbera jamesonii Bolus) is a perennial herbaceous flower of the genus Gerbera in the Compositae family. It is widely planted in my country because of its large flowers, bright flowers, full color and long flowering period. At present, tissue culture rapid propagation technology is usually used to provide high-quality disease-free and non-toxic gerbera seedlings for production. The existing tissue culture rapid propagation technology mainly includes two stages: culture room culture and cutting culture outside the culture room. The culture room culture mainly includes induction culture, proliferation culture and rooting culture of explants. The culture outside the culture room mainly involves culturing in the c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 单芹丽杨春梅王继华李绅崇屈云慧吴丽芳汪国鲜李金泽曹桦许凤
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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