368 results about "Soil borne" patented technology

The invention discloses bacillus cereus and application thereof, and belongs to the technical field of microorganisms. The bacillus cereus SNF7 is separated from a saline-alkali soil sample, has the functions of resisting salt, fixing nitrogen and promoting growth, has an obvious inhibition effect on some common soil-borne diseases in agriculture, such as pathogenic bacteria of peanut fruit rot, tomato root rot and the like, has multiple functions of resisting salt, fixing nitrogen, biologically preventing and controlling and the like, and has a wide application prospect. The method can be widely applied to crop planting in large saline-alkali soil in China.

The invention discloses a microbial agent for preventing and treating soil-borne diseases of food crops and a preparation method of the microbial agent, and belongs to the technical field of microorganisms. The microbial inoculum comprises three functional strains, namely pseudomonas guayensis, paenibacillus polymyxa and trichoderma koningiopsis, the three strains are mixed in equal proportion and cooperate with one another, so that the microbial inoculum has a remarkable disease-resistant effect, has an antagonistic effect on various diseases of wheat and corn, can be used for preventing and treating wheat root rot, take-all and banded sclerotial blight as well as corn stem rot and banded sclerotial blight, and has a wide application prospect. Particularly, the inhibition effect on the take-all disease of wheat is optimal. Meanwhile, the coating agent and the auxiliaries are used, so that the acting durability and stability of the microbial inoculum are improved. The microbial agent is matched with a conventional base fertilizer for use, use of chemical bactericides is reduced, growth of common pathogenic bacteria of food crops is effectively inhibited, and the yield of the food crops is increased.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The invention relates to the field of crop cultivation, and particularly discloses a multi-crop interplanting method with a high land utilization rate. According to the method, a multi-crop interplanting land distribution scheme is designed on the basis of user planting requirements, planting molds are built on land parcels with serious soil problems, and the planting molds are filled with crop growth supporting materials to serve as planting soil; planters can interplant various crops in the planting soil according to a multi-crop interplanting land distribution scheme, crops with serious soil-borne diseases, such as solanaceae or medicinal materials, can be planted on the same land for many years, planting can be carried out in land parcels with serious soil problems, the land utilization efficiency is improved, and the planting cost is reduced. The crop yield per unit land area can be increased in an interplanting mode, land resources are fully utilized, meanwhile, the planting production cost is reduced, and the economic output per unit area is increased.

The invention relates to a cultivation nutrient medium and a preparation method thereof. According to the nutrient medium, peat is completely abandoned, the coco coir, the straw and the biochar are combined to reduce ecological burden, the nutrient medium is an environment-friendly medium, the biochar fixes carbon and reduces emission, and the soil structure can be improved after long-term use; vermiculite improves the water-holding capacity, perlite and straw form a stable pore structure, and the ventilation porosity reaches 25% or above. Wormcast and a microbial agent synergistically release nitrogen, phosphorus and potassium, and the fertilizer efficiency is lasting. The microbial agent can inhibit soil-borne diseases such as fusarium and reduce the death rate of cultivated seedlings. The biochar adsorbs salt and toxins, and the EC value is stabilized at 1.2-1.8 mS / cm. The cost of the selected raw materials is reduced by 35% compared with that of a traditional substrate, and the substrate is suitable for large-scale production, suitable for vegetable seedling culture, potted flowers, organic farms and soilless culture systems, and especially suitable for saline-alkali soil or continuous cropping obstacle areas.

The invention belongs to the technical field of microorganisms, and particularly relates to a P.adami FJ08 strain, a fungicide, a preparation method and application thereof, which can be used for inhibiting soil-borne diseases of radix pseudostellariae. The invention discloses a P.aryabhattai FJ08, the P.aryabhattai FJ08 is preserved in the Microbial Culture Collection Center of Guangdong Province, the preservation number is GDMCC No: 65907, and the preservation date is March 24, 2025. The P.aryabhattai FJ08 has the advantages that the P.aryabhattai FJ08 can be used for preparing the P.aryabhattai FJ08; the P.aryabhattai FJ08 is separated from the radix pseudostellariae seed ginseng, is safe and reliable, and has the capabilities of inhibiting the growth of fusarium oxysporum, producing IAA, producing ammonia, dissolving potassium and dissolving phosphorus, and the growth of pathogenic fusarium oxysporum can be effectively inhibited by soaking the radix pseudostellariae seed ginseng with the P.aryabhattai FJ08.

The invention belongs to the technical field of biological control, and particularly relates to a pseudomonas flax DY-31 strain, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the pseudomonas lini (Pseudomonas lini) DY-31 is CGMCC (China General Microbiological Culture Collection Center) No.34088. The pseudomonas lini (Pseudomonas lini) DY-31 is The pseudomonas flax DY-31 or the biocontrol inoculant thereof is applied to prevention and treatment of plant soil-borne diseases, specifically including peanut black rot, peanut southern blight, peanut sclerotinia rot, peanut leaf rot sclerotinia rot, peanut basal rot and wheat stem basal rot. The pseudomonas flax DY-31 provided by the invention is an important strain resource, can be used for creating a biopesticide preparation, and is used for green prevention and treatment of soil-borne diseases of peanuts and wheat. In addition, the DY-31 strain also has various growth promoting functions, and has important development and application values in the aspects of agricultural biological control and plant growth promoting.

The invention discloses a myrosinase-producing Cupriavidus strain and application of the myrosinase-producing Cupriavidus strain in prevention and treatment of soil-borne diseases. The preservation number of the Cupriavidus sp. Strain is CGMCC (China General Microbiological Culture Collection Center) NO.34736. The Cupriavidus sp. Strain is a Cupriavidus sp. Strain. The invention further provides application of the strain in prevention and treatment of soil-borne fungal diseases and a soil disinfection method. According to the soil disinfection method provided by the invention, the amount of isothiocyanate substances generated in a biological fumigation technology is greatly increased, and a remarkable inhibition effect on soil-borne pathogens can be achieved.

Provided are a method for controlling a soilborne disease in which a load on an environment and a human body is reduced, and a novel predatory bacterium therefor.　Provided are: a predatory bacterium or a variant thereof selected from the group consisting of bacteria of the genus Bacteriovorax and bacteria of the genus Bdellovibrio which selectively prey on a pathogenic bacterium belonging to the family Burkholderiaceae, the family Pectobacteriaceae, or the family Lysobacteraceae; a method for controlling a soilborne plant disease caused by the pathogenic bacterium, the method characterized in that the predatory bacterium or variant thereof is applied to a seed, root, or underground stem of a plant, rhizosphere soil, or soil; and a composition.

The invention discloses a coated slow-release solid water-soluble fertilizer as well as a preparation method and application thereof. The coated slow-release solid water-soluble fertilizer comprises the following components in parts by weight: 15-20 parts of a nitrogen source, 8-12 parts of a phosphorus source, 15-25 parts of a potassium source, 3-8 parts of humic acid and 10-15 parts of charcoal powder, the composite material comprises the following components in parts by weight: 0.5-1.5 parts of functional microorganisms, 1-2 parts of microbial metabolite, 2-5 parts of seaweed extract and 5-8 parts of chitosan-gelatin composite material. The functional microorganisms are bacillus subtilis, bacillus licheniformis and azospirillum which are adsorbed on the charcoal powder. The coated slow-release solid water-soluble fertilizer provided by the invention has the effects of supplying nutrients and preventing and treating soil-borne diseases, achieves multiple effects by one fertilizer, and can improve the crop nutrient utilization rate, inhibit the soil-borne diseases and promote the diversity of soil microorganisms.

The invention provides a microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening and a preparation method of the microbial agent type bio-organic fertilizer, and belongs to the technical field of organic fertilizers. The microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening comprises the following raw materials: an organic material, a microbial agent, an inorganic nutritional ingredient, vinasse and an additive, wherein the organic material is composed of livestock and poultry manure, crop straw, tobacco waste and a fermentation inoculant; the additive is prepared from humic acid, seaweed extract and amino acid. The organic fertilizer contains rich organic materials and humic acid, the content of organic matters in soil can be increased, the granular structure of the soil can be improved, the growth of soil-borne pathogenic bacteria can be inhibited by the added multiple beneficial microorganisms, the micro-ecological environment of the soil can be improved by the photosynthetic bacteria, and the incidence rate of diseases such as tobacco black shank and bacterial wilt can be reduced.

The invention provides a cultivation method for promoting growth of tricholoma matsutake by using a biological activation substrate and application of the cultivation method. The invention provides a tricholoma matsutake cultivation process which takes bamboo shoot shells as a main material and is matched with a growth-promoting strain vv6. The method comprises the following steps: crushing and pre-wetting raw materials, carrying out aerobic high-temperature composting, cooling to a safety window, adding vv6 for pre-activation, inoculating tricholoma matsutake, covering rice husks, and controlling a fruiting environment. A ground isolating membrane structure is overlaid on a field cultivation scene, a soil-borne disease and pest ascending channel is cut off in a physical mode, and experimental results show that the red tricholoma matsutake planted through the method is higher in yield, the nutrient content of sporocarp is richer, and application and popularization are better facilitated.

The invention belongs to the technical field of agricultural microbial agents, and relates to an agricultural liquid microbial agent preparation method and a microbial agent, the method comprises the following steps: mixing bacillus amyloliquefaciens and trichoderma reesei, and carrying out primary aerobic fermentation; mixing a product subjected to primary aerobic fermentation with lactobacillus acidophilus subjected to expanding culture, and carrying out secondary micro-aerobic fermentation; and carrying out membrane separation treatment on the product subjected to secondary microaerobic fermentation, and concentrating the bacterial liquid. According to the microbial agent, through the synergistic effect of multiple strains, the inhibition rate of soil-borne pathogenic bacteria is increased, the occurrence rate of crop rhizosphere diseases is reduced, and then the yield and quality of crops are synergistically optimized. High-activity flora is reserved through gradient fermentation and membrane separation technologies, the continuous colonization capacity of thalli in deep soil is maintained in combination with biochar, a long-term stable field protection barrier can still be formed through flora interaction even if mechanical turning is not needed, and therefore disease prevention and control are achieved under the no-tillage or minimal-tillage condition.

The invention discloses a bacteriophage cocktail for preventing and treating soil-borne bacterial wilt, which comprises four strains of ralstonia solanacearum obligate lytic bacteriophages in the following (1)-(4): (1) bacteriophage LC5 with the preservation number of GDMCC NO: 65460-B1; (2) a bacteriophage GC3, the preservation number of which is GDMCC NO: 65459-B1; (3) bacteriophage YH1, the preservation number of which is GDMCCNO: 65461-B1; and (4) bacteriophage YH3, the preservation number of which is GDMCC NO: 65462-B1. Indoor related experiments show that the phage cocktail composed of the four phages GC3, LC5, YH1 and YH3 can effectively inhibit growth of tobacco ralstonia solanacearum, and the effect of the phage cocktail is better than that of a single phage; pot experiments also show that the bacterial wilt can be effectively inhibited.

The invention discloses a bacterial strain for antagonizing multiple pathogenic fungi of potatoes and simultaneously promoting growth and application of the bacterial strain, and relates to the field of microbial agents, the bacterial strain is bacillus wiedmannii Bw34, the bacterial strain is preserved in China General Microbiological Culture Collection Center, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is November 11, 2024, and the preservation number is CGMCC NO. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The compound is used for preparing a fungicide for antagonizing potato pathogenic fungi and a fungicide for promoting potato growth. The Bw34 has the effect of comprehensively preventing and treating potato soil-borne diseases, meanwhile, potato growth and nutrient absorption are promoted, and the application prospect is good.

The invention relates to a pepper cultivation device capable of reducing soil-borne diseases and a using method thereof.The pepper cultivation device comprises a mounting plate and further comprises a first plate body, a second plate body and a rotating plate located between the first plate body and the second plate body, and a first connecting shaft is rotationally connected with the first plate body and the second plate body and fixedly connected with the rotating plate; the side wall of the rotating plate is provided with a containing groove for placing seedling cups, the first plate body is provided with a liquid injection structure with the output end facing the containing groove, the liquid injection structure is located above the rotating track of the containing groove, and the second plate body is provided with a jacking plate located below the liquid injection structure. The rotating disc rotates to be matched with the jacking plate, when seedling cups in the containing grooves rotate and move to the position between the jacking plate and the liquid injection structure, the heights of the seedling cups are higher and higher, the liquid injection structure is promoted to be inserted into soil in the seedling cups, and therefore remediation liquid is injected into the soil instead of being sprayed on the surface of the soil only in the prior art. The treatment effect on the soil is effectively improved.

The invention discloses a prevention and control method for tobacco soil-borne diseases based on a mushroom waste substrate and application. Belongs to the technical field of tobacco soil-borne disease prevention and control. According to the method, the tobacco soil-borne diseases are prevented and controlled by combining the mushroom waste substrate with the biological agent, the added mushroom waste substrate can increase the antibacterial activity of biocontrol bacteria, the control effect of the biocontrol bacteria is remarkably improved, the application direction of the mushroom waste substrate is widened, and maximum utilization of mushroom waste substrate resources is achieved on the premise of environment friendliness.

The invention relates to the technical field of microorganisms, and particularly discloses a microbial preparation for improving soil-borne diseases of soil and a preparation method thereof.The microbial preparation is prepared from modified biochar, a compound microbial agent, plant essential oil, chitosan and konjaku flour; the preparation method comprises the following steps: soaking the modified charcoal in the compound microbial agent, adding the konjaku flour, the chitosan and the plant essential oil, uniformly stirring and mixing, and then freeze-drying to obtain the required microbial preparation for improving the soil-borne diseases of the soil. The microbial preparation prepared by the invention has the beneficial effects of promoting plant root growth and improving soil-borne diseases.

The invention discloses application of cobalamin and beneficial bacteria in prevention and control of tomato soil-borne bacterial wilt. The beneficial bacterium is Chryseobacterium CHR6, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) The invention discloses a synbiotics for preventing and controlling tomato soil-borne bacterial wilt. The synbiotics consist of beneficial bacteria and cobalamin, and the beneficial bacterium is Chryseobacterium CHR6. The invention discloses a method for preventing and controlling tomato soil-borne bacterial wilt by matching cobalamin and beneficial bacteria, which comprises the following steps: 7-10 days after transplanting tomato seedlings, adding synbiotics into soil by a root irrigation method, adding 10-20 mL of cobalamin solution with the concentration of 5-10 nM into each tomato seedling, and inoculating 10-20 mL of beneficial bacteria suspension with the concentration of 1 * 10 < 7 >-1 * 10 < 9 > CFU / mL. By adding cobalamin, inhibition of growth of ralstonia solanacearum through siderophore mediation of Chryseobacterium CHR6 can be remarkably enhanced, and occurrence of tomato soil-borne bacterial wilt is reduced.

The invention discloses a process method for preparing an organic fertilizer for efficiently inhibiting soil-borne diseases from pig manure, and the pig manure is converted into the efficient and environment-friendly organic fertilizer through a series of optimization treatment steps. The technology comprises the following key steps: firstly, pig manure is subjected to collection, pretreatment and heavy metal detection, and it is ensured that raw materials meet the environmental protection standard; then adjusting the ratio of pig manure to auxiliary materials (such as pepper oil residue, tobacco stem powder, straw powder, sawdust and the like), and optimizing the carbon nitrogen ratio and the moisture content; adding a compound microbial agent into the fermentation tank, controlling the fermentation temperature, humidity and oxygen concentration, and ensuring the microbial activity; through graded fermentation, deodorization and germ killing treatment, peculiar smell and harmful substances are effectively removed; the nutrient content and the stability of the fertilizer are further improved through the steps of drying, crushing, curing and the like; and finally, reinforced modification is performed, and mineral substances, trace elements and a biocontrol inoculant are added, so that the finished product fertilizer has a good soil-borne disease inhibition effect and a good soil improvement effect.

The invention relates to the technical field of fertilizer preparation, and particularly discloses a biological organic fertilizer rich in deep sea enzymolysis fish protein and a preparation method of the biological organic fertilizer. The biological organic fertilizer is prepared from the following components in parts by weight: 35 to 45 parts of organic fertilizer (containing deep sea enzymolysis fish protein, tobacco powder, meat powder and bone meal), 6 to 15 parts of compound microorganism (such as bacillus licheniformis and the like), 5 to 10 parts of medium element fertilizer, 1 to 2 parts of trace element fertilizer, 0.1 to 1 part of xanthan gum and sulfur suspension, 30 to 35 parts of outer layer fertilizer (containing humic acid, urea and the like) and 0.1 to 0.5 part of anti-blocking agent. The preparation method comprises the following steps: separately fermenting microorganisms to prepare bacterial powder; mixing, crushing and granulating the raw materials to form master batches; spraying xanthan gum and sulfur suspension on the surface of the master batch to form a film; spraying the outer-layer fertilizer slurry for several times; and finally, an anti-blocking agent is sprayed, and air drying is conducted. The deep sea enzymolysis fish protein is taken as a core, the balance of quick-acting fertilizer efficiency and slow release is realized, the soil structure can be improved, the crop growth is promoted, the stress resistance and the fertilizer utilization rate are improved, the soil-borne diseases are inhibited, and the method has the advantages of high efficiency, environment friendliness, economy, practicability and the like.

The invention discloses paenibacillus alvei NNUB1, a fungicide and application of the paenibacillus alvei NNUB1. The paenibacillus alvei NNUB1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.28005. The invention further discloses a preparation method of the paenibacillus alvei NNUB1. The newly separated paenibacillus alvei NNUB1 has excellent activity of resisting soil transmission plant pathogens, not only can antagonize various fusarium fungi, but also can effectively inhibit lauraceae lauraceae; the strain also has the characteristic of promoting plant growth, and the biomass of plants can be obviously improved; test data show that the strain can effectively inhibit soil-borne pathogens of continuously-cultivated soil, remarkably promote crop growth and has an excellent application prospect.

The invention discloses a method for preparing a humic acid water-soluble fertilizer from caragana microphylla slurry and melaleuca alternifolia essential oil, and relates to the technical field of fertilizers. The problems that raw material resources for preparing the humic acid water-soluble fertilizer are limited, and the preparation cost is high are solved. The method specifically comprises the following steps: preparing raw materials: caragana microphylla, melaleuca alternifolia essential oil and an auxiliary reagent; preparation and biotransformation of caragana microphylla pulp; emulsifying the melaleuca alternifolia essential oil; performing fertilizer compounding and quality regulation and control; and processing and storing finished products. The method has the characteristics of efficient utilization of resources, function synergy and environmental friendliness, the caragana microphylla in the preparation raw materials is a waste branch in a desert area and can turn waste into wealth, the essential oil is a natural extract and replaces a chemical synthesis synergist in an existing preparation method, soil is improved through humic acid, soil-borne diseases are inhibited through the essential oil, root absorption is promoted through the essential oil, and the soil-borne diseases are prevented from being damaged. Meanwhile, the humic acid water-soluble fertilizer is free of harmful residues and suitable for green agriculture.

The invention discloses a streptomycete N1 strain for inhibiting phytopathogen, a biocontrol inoculant and application of the biocontrol inoculant. The classification name of the Streptomyces sp. Is Streptomyces sp., the Streptomyces sp. Is preserved in the China Center for Type Culture Collection, and the preservation number of the Streptomyces sp. Is CCTCC NO: M 20242700. Experiments prove that the streptomyces N1 strain can significantly inhibit a variety of phytopathogens such as fusarium oxysporum, botryosphaeria dothidea, fusarium graminearum, acremonium nucleatum, botrytis cinerea, sclerotinia sclerotiorum and phytophthora nicotianae. A biological control fungicide prepared from the streptomyces N1 strain can effectively control plant soil-borne diseases, has the characteristics of high efficiency, environmental protection, low cost and easiness in preparation, is an optimal scheme for agricultural sustainable development, and has a good and wide market application prospect.

The invention discloses a Trichoderma harzianum-containing biocontrol fertilizer and a preparation method thereof, and belongs to the technical field of mixed fertilizers, the fertilizer comprises composite matrix particles (containing an organic carrier, an inorganic fertilizer and trace elements), a microsphere coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and a water-soluble fertilizer coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and the outermost layer is a biodegradable protective film (modified starch / PVA / PLA). The preparation method comprises the following steps: granulating a matrix, preparing a bacterial suspension, forming bacteria-containing gel microspheres (the spore recovery rate is greater than or equal to 95%), attaching the microspheres to the matrix, and coating the outer layer. According to the structure, the survival rate of viable bacteria stored at room temperature for 120 days is larger than or equal to 68.3%, the rhizosphere bacterial count is larger than or equal to 4.58 * 10 < 6 > CFU / g soil after the viable bacteria are applied to the soil for 30 days, soil-borne diseases are remarkably inhibited, and the crop yield is increased by 13.5% (cucumber yield is increased by 13.5%).

The present invention discloses a method and device for preventing and controlling soil-borne diseases by burning crop stubble. The method comprises the following steps: S1. cutting and collecting the shallow soil layer where the stubble is located; S2. separating and processing the collected shallow soil layer, removing the soil carried by the stubble, and obtaining separated stubble and soil; S3. burning the separated stubble, and heating the separated soil with the high-temperature flue gas generated by the combustion; S4. mixing the ash from the burned stubble with the heated soil, and then returning the ash to the field. The present application utilizes the high calorific value characteristics of crop stubble, and the high-temperature flue gas generated by its combustion heats the surface soil, thereby killing the stubble and the diseased and insect eggs and pathogenic microorganisms in the rhizosphere soil carried by the stubble. It can also form a certain thermal contact killing effect on the diseases and insect pests in the soil, reduce the degree of damage caused by the diseases and insect pests, and thus achieve the purpose of green prevention and control of diseases and insect pests.

The invention relates to paenibacillus strongly ZKSW001 as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. The paenibacillus pumilus ZKSW001 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36076, the preservation date is September 25, 2025, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides application of the paenibacillus strongly ZKSW001 in inhibition of harmful bacteria in soil, prevention and treatment of root-knot nematode and promotion of plant growth. The paenibacillus strongly ZKSW001 disclosed by the invention not only can promote the growth of crops and realize weight loss and efficiency improvement, but also can control the occurrence of soil-borne fungal and bacterial diseases, and can inhibit the growth of root-knot nematode. Chemical pesticide application can be reduced, pesticide residues are reduced, and sustainable development of agricultural economy is promoted.

The invention relates to the technical field of biological agents, and particularly discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent provided by the invention comprises compound bacillus, a carrier and a coating agent, the composite bacillus comprises bacillus velezensis, bacillus atrophaeus and bacillus firmus with the viable count ratio of (3-7): (1-4): 2; the viable count of the compound microbial agent is greater than or equal to 5 * 10 < 9 > cfu / g; the invention also provides a preparation method of the compound microbial agent. The preparation method comprises the following steps: strain fermentation, carrier adsorption, granulation and enveloping and low-temperature drying. The compound microbial agent provided by the invention can improve the soil structure, enhance the soil fertility and inhibit soil-borne diseases, so that the growth condition of crops is improved, and the quality and yield of the crops are improved.