162 results about "Soil borne" patented technology

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention discloses a method for preparing a humic acid water-soluble fertilizer from caragana microphylla slurry and melaleuca alternifolia essential oil, and relates to the technical field of fertilizers. The problems that raw material resources for preparing the humic acid water-soluble fertilizer are limited, and the preparation cost is high are solved. The method specifically comprises the following steps: preparing raw materials: caragana microphylla, melaleuca alternifolia essential oil and an auxiliary reagent; preparation and biotransformation of caragana microphylla pulp; emulsifying the melaleuca alternifolia essential oil; performing fertilizer compounding and quality regulation and control; and processing and storing finished products. The method has the characteristics of efficient utilization of resources, function synergy and environmental friendliness, the caragana microphylla in the preparation raw materials is a waste branch in a desert area and can turn waste into wealth, the essential oil is a natural extract and replaces a chemical synthesis synergist in an existing preparation method, soil is improved through humic acid, soil-borne diseases are inhibited through the essential oil, root absorption is promoted through the essential oil, and the soil-borne diseases are prevented from being damaged. Meanwhile, the humic acid water-soluble fertilizer is free of harmful residues and suitable for green agriculture.

The invention relates to the technical field of agricultural microorganisms, in particular to trichoderma asperellum and application thereof.The trichoderma asperellum comprises at least one of the following trichoderma asperellum TA727, the trichoderma asperellum TA727 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO. 63859. The trichoderma asperellum has the advantages that the trichoderma asperellum can be used for preparing the trichoderma asperellum; the trichoderma asperellum is high in sporulation quantity and strong in acid resistance, and has a relatively good pathogenic bacterium inhibition effect under an acidic condition, and volatile substances of the trichoderma asperellum also have an antibacterial effect. The trichoderma asperellum can effectively inhibit pathogenic bacteria in an acid soil environment and soil-borne diseases caused by the pathogenic bacteria, meanwhile, industrial production can be facilitated, and the trichoderma asperellum has wide application prospects.

This invention discloses a liquid-solid two-step composite seed coating material and its coating method for peanuts and garlic, belonging to the field of agricultural seed treatment technology. The coating material consists of a liquid activating seed soaking agent and a solid protective coating powder, with modified humic acid, biochar, and polycrystalline energy-concentrating materials as core functional components, and contains no chemical pesticides. It employs a two-step process: first, liquid soaking and activation, followed by solid powder coating to form an activation + protection composite functional layer on the seed surface. Multiple repeated comparative experiments show that this invention can significantly advance the germination of peanut seeds, with the total root volume approximately twice that of the uncoated group, significantly improving the survival rate, while simultaneously reducing the incidence of soil-borne diseases and increasing yield. This invention is green, safe, has stable effects, and is highly targeted, making it suitable for large-scale planting and promotion of peanuts and garlic.

The invention discloses application of bacillus subtilis ZY56 in prevention and treatment of plant soil-borne diseases, and belongs to the technical field of plant protection. In order to solve the problem that in the prior art, efficient, safe and environment-friendly prevention and treatment measures are lacked in production of soil-borne diseases, the invention provides a strain of bacillus subtilis ZY56 with the preservation number of CGMCC No. 36896; the bacillus subtilis ZY56 provided by the invention has a broad-spectrum antagonistic effect on various soil-borne diseases, also has growth promoting potential, has the characteristic of high biological safety, is harmless to the environment, people, livestock and crops, and meets the requirements of green agriculture and sustainable development.

The invention discloses a compound microbial agent for preventing and treating muskmelon fusarium wilt and application of the compound microbial agent, and belongs to the field of biological prevention and treatment. The microbial inoculum is composed of pseudomonas aeruginosa and bacillus subtilis, the dosage form is a liquid microbial inoculum, and the effective viable count is greater than 10 billion / ml. The two strains are high in sporulation quantity and strong in rhizosphere colonization capacity, have synergistic bacteriostasis and growth promotion effects, effectively inhibit fusarium oxysporum of muskmelons through a plurality of mechanisms such as competition, secondary metabolite generation and system resistance induction, and have a remarkable inhibition effect on fusarium oxysporum of cucumbers, watermelons and tomatoes, soil-borne pathogenic bacteria such as phytophthora capsici and the like. The microbial agent is broad-spectrum, efficient and long in lasting period, and can promote propagation of rhizosphere effective microbial communities, reduce the dosage of chemical pesticides, relieve continuous cropping obstacles and promote green and high-quality development of the watermelon and melon industry.

To provide a new agent for controlling soil-borne plant diseases, containing a microorganism as an active ingredient.SOLUTION: An agent for controlling a soil-borne plant disease, comprising, as an active ingredient, a Pseudomonad bacterium having a 16SrRNA genome comprising a nucleotide sequence having 96% or more sequence identity to a nucleotide sequence represented by SEQ ID NO: 1, a Pseudomonad bacterium having a 16SrRNA genome comprising a nucleotide sequence having 98% or more sequence identity to a nucleotide sequence represented by SEQ ID NO: 2, or a combination thereof.SELECTED DRAWING: None

The invention relates to application of a dazomet liquid preparation in soil treatment in the field of pesticides. The liquid preparation can be used for preventing and treating true and bacterial diseases, nematodes and weeds of crops, and has a remarkable synergistic effect on soil-borne bacteria, fungal diseases, root-knot nematodes and field weeds. The pesticide application mode can be selected from one or more of drip irrigation, flushing application, mist spraying and soil layer treatment, and the pesticide can be directly used or used after being diluted for multiple times during pesticide application. Besides, the core advantage of the application is that the application limitation of the traditional dazomet preparation is broken through, and the dazomet preparation is suitable for wide regions, especially suitable for low-temperature regions and environments with the soil temperature being lower than 10 DEG C, and is especially preferably applied to rice seedling raising fields in low-temperature regions; multiple operation modes such as soil mixing, pulping and rice hull mixing can be adopted in a low-temperature scene, the operation is convenient and fast, the actual field requirements are met, and the environmental adaptability and the application flexibility of the dazomet preparation are remarkably improved.

The application belongs to the technical field of plant disease prevention and treatment, and relates to a determination method for visually and rapidly evaluating the effect of pesticides on inhibition of soil-borne pathogen infection on crop root systems. Agar powder is added to deionized water and boiled until dissolved, sterilized by high-pressure steam and cooled to 60-65 DEG C to obtain an agar culture medium, and the agar culture medium is poured into a culture dish and continuously cooled to solidification. A double-layer filter paper is laid on the solidified culture medium, pre-germinated seeds are placed above the filter paper and cultivated until the root system expands. After the root system of the seedling expands, the pathogen is inoculated and pesticide liquid is applied; and the activity of the pesticide on the pathogen is evaluated. The advantages of the application are that rapid inoculation can be realized, symptoms can be observed in real time, nematodes can be rapidly inoculated and dyed, pesticides can be applied at different stages before, during and after pathogen infection, and the protective and therapeutic activities of the pesticide can be rapidly evaluated. The application greatly facilitates the related research on pathogen or nematode infection, and provides a rapid and efficient method for evaluating the activity of the pesticide on the soil-borne pathogen.

This application discloses a Bacillus subtilis strain JTFB-01 and its applications, belonging to the field of microbiology. The Bacillus subtilis strain JTFB-01 has the accession number CGMCC No. 37199 and is deposited at the China General Microbiological Culture Collection Center on December 25, 2025. Field trials show that this inoculant, applied through seed treatment, achieves control effects of 68.20%, 71.73%, and 72.15% against wheat take-all, sheath blight, and root rot, respectively, and significantly increases wheat plant height and yield. It also shows a field control effect of over 50% against cotton Verticillium wilt, with a yield increase of over 17%. This inoculant effectively controls soil-borne diseases and overcomes crop replanting obstacles through multiple mechanisms, including inhibiting pathogens, colonizing the rhizosphere, and promoting growth, thus possessing significant agricultural application value.

The invention provides bacillus megatherium for preventing and treating soil-borne diseases of crops, a microbial inoculum and application of the bacillus megatherium and the microbial inoculum. The bacillus megaterium HN-2 disclosed by the invention is classified and named as bacillus megaterium, the bacillus megaterium HN-2 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the bacillus megaterium HN-2 is CGMCC No.34657. A test result shows that the antibacterial effect of the bacillus megaterium HN-2 on four kinds of fusarium and tobacco black shank bacteria reaches 60.98%-71.12%; the indoor nematode killing effect on wheat cyst nematode and tomato root-knot nematode reaches 100%. The optimized binder, wetting agent, stabilizing agent and disintegrating agent are respectively starch, lauryl sodium sulfate, CaCO3 and AlCl3; after seed dressing and broadcasting, the field cyst decline rates of wheat cyst nematode and soybean cyst nematode are 60.22% and 52.58% respectively, the field prevention effects of wheat root rot and soybean root rot are 69.50% and 54.44% respectively, and the biocontrol inoculant has wide application potential.

The invention discloses a carbon cap and a preparation method thereof.The carbon cap comprises a carbon cap body, the carbon cap body is a cube with a cavity and an opening at one end, a cylinder or a sphere with a partial notch, and the cavity is used for containing root tissue of a plant; the carbon cap body comprises 70-85 parts of biochar, 10-15 parts of an adhesive and 5-15 parts of an alkaline auxiliary agent. The carbon cap is a high-porosity, neutral and slightly alkaline seedling protection sleeve, has multiple functions of water retention, heat preservation, bacteriostasis and the like, provides a safe soil environment for growth of seedlings planted or transplanted, prevents damaged root systems from being infected by soil-borne diseases during planting or transplanting, ensures the survival rate after transplanting and good growth and development, and improves the survival rate of the seedlings. The method has good practicability and application value.

The application discloses an intelligent management system and method applied to strawberry disease identification, relates to the technical field of intelligent management of strawberry soil-borne disease prevention and control, and collects standardised shed body basic information and soil pathogen data; determines the shed body as a new shed, an old shed and a transition shed based on planting years and pathogen threshold values; generates a differentiated prevention and control scheme according to the shed type, wherein the scheme comprises soil treatment and above-ground prevention and control measures; then, the scheme is converted into a standardised execution guide containing pesticide calculation and is pushed, the pesticide calculation adopts corresponding formulae; finally, pathogen changes are monitored every month; and the shed type and the scheme are dynamically adjusted according to the monitoring results.

The invention discloses a bacillus PY4 strain capable of efficiently antagonizing various phytopathogens and application of the bacillus PY4 strain, and belongs to the technical field of biological prevention and control of plant diseases. The strain has stable morphological characteristics, and is identified according to a 16S rRNA (ribosomal Ribonucleic Acid) sequence, and the strain is a Paenibacillus bacterium. An in-vitro confrontation test proves that the PY4 can effectively inhibit pathogenic fungi such as sclerotinia sclerotiorum, fusarium graminearum, pseudocauda sp, alternaria alternata and sclerotium rolfsii. Field application results show that the PY4 has a good prevention and treatment effect on sclerotinia rot of colza. The invention further provides a biocontrol agent based on the strain and an application method of the biocontrol agent. The biocontrol agent can be used for green prevention and control of soil-borne and leaf-borne fungal diseases of crops.

The application discloses a granular fertilizer for improving saline-alkali soil based on traditional Chinese medicine active matter cooperation and multi-stage endogenous controlled release, and a preparation method and application thereof. Fermented straw is used as a core organic carrier, and milk and a traditional Chinese medicine (Astragalus, licorice, honeysuckle and the like) active matter collection rich in amino acids and proteins are introduced innovatively in the fermentation process, so that the quality and biological activity of the carrier are improved significantly. The carrier can not only be dissolved quickly to create a high-activity microenvironment for functional microorganisms, but also cooperates with the traditional Chinese medicine active matter to build a whole-chain ecological restoration network covering soil chemical improvement, biological cycle acceleration, plant stress resistance induction and soil-borne disease prevention. In combination with a four-fold endogenous protection system of microorganisms, an internal intercommunication porous structure formed by low-temperature compression and quantitative regulation of the coating thickness, the functional bacterial population has an ultrahigh survival rate, the saline-alkali and biological obstacles are improved synchronously, and the nutrient intelligent release is realized.

The invention discloses a bacteriostatic slow-release water-saving type hole storage ball based on industrial hemp stalks and a preparation method thereof.The hole storage ball is prepared from, by mass, 5-10 parts of industrial hemp stalk powder, 30-50 parts of montmorillonite powder, 2-10 parts of activated carbon, 5-10 parts of a water-retaining agent, 5-20 parts of a water-soluble nitrogen-phosphorus-potassium compound fertilizer and 20-40 parts of water; the montmorillonite powder, the activated carbon and the water-soluble nitrogen-phosphorus-potassium compound fertilizer are premixed and then secondarily mixed with the industrial hemp stalk powder and the water-retaining agent, and granulation molding is performed to prepare the hole storage ball. According to the invention, antibacterial substances such as cannabinol naturally contained in the waste industrial hemp stalks are fully utilized, so that the fertilizer block is endowed with an internal plant health-care function, the hole storage ball effectively inhibits soil-borne pathogenic bacteria such as fusarium and rhizoctonia solani, the phenomena of root rot and seedling death of crops are reduced, the use of chemical pesticides is reduced, and the production cost is reduced. The problems of crop water retention, bacteriostasis and soil improvement can be solved simultaneously, waste is turned into wealth, and the method is suitable for popularization and application.

The invention relates to the crossing field of agricultural biological control and fertilizer technology, and discloses a compound microbial pesticide fertilizer capable of simultaneously controlling root-knot nematode and soil-borne fungal diseases, the pesticide fertilizer has a core-shell structure, and comprises an inner inorganic nutrition core, a middle isolation buffer layer and an outer functional bacterial layer; the raw materials comprise inorganic nutrition core particles, a biological activation organic carrier, composite functional bacterial powder and a modified slow-release binder. Wherein the binder is prepared from oxidized starch and PVA through borax crosslinking, so that the particle strength is enhanced; the carrier is fermented and activated, and synergistic components such as azadirachtin are added. An organic isolation buffer layer is constructed through a layered coating process, osmotic pressure stress of inorganic salt on microorganisms is effectively blocked, and the problem of strain inactivation is solved; the synergistic compounding of paecilomyces lilacinus, pochonia chlamydosporia, trichoderma harzianum and bacillus subtilis is utilized to realize the comprehensive prevention and control of root-knot nematode and soil-borne fungal diseases, and has the functions of nutrient slow release and crop resistance induction.

The invention discloses a soil remediation fertilizer and a preparation method thereof, and belongs to the technical field of soil remediation. The fertilizer is composed of a nitrogen-phosphorus-potassium fertilizer and a remediation agent; the mass ratio of the nitrogen-phosphorus-potassium fertilizer to the repairing agent is (550-750): (250-450). Natural raw materials are adopted, and the advantages of being environmentally friendly, non-toxic and harmless are achieved. The fertilizer provided by the invention is used for agricultural soil improvement, can restore soil fertility, has improvement and inhibition effects on soil hardening and soil-borne diseases, and also can play a role in fixing heavy metals in soil, so that the seedling death condition of crops caused by the heavy metals is reduced, and the survival rate of the crops is increased.

This application discloses a strain of *Priestia taiwanensis* W178 and its application in controlling soil-borne fungal diseases. This strain was deposited at the China Center for Type Culture Collection (CCTCC) on December 29, 2025, with accession number CCTCC NO:M 20253047. The strain exhibits significant antagonistic activity against 11 soil-borne pathogenic fungi, including *Sclerotinia sclerotiorum* and *Fusarium graminearum*, with an inhibition rate reaching up to 91.10%. This application also establishes a fermentation process for this strain, resulting in a spore count of 19.3 × 10⁻⁶ after fermentation. 8 CFU / mL. Pot experiments showed that the fermentation broth, diluted 10 times, was significantly effective in controlling rapeseed sclerotinia stem rot and could effectively inhibit the spread of lesions. This application is the first to discover the broad-spectrum biocontrol potential of *Priscilla taiwanensis* and provides an active strain and its fermentation product that can be developed into microbial agents, bio-organic fertilizers, or compound microbial fertilizers, suitable for the green control of various soil-borne fungal diseases such as rapeseed sclerotinia stem rot and wheat stem rot.

The invention discloses a composition containing phenazino-1-carboxylic acid and spinosad and application of the composition. The active ingredients of the composition are phenazino-1-carboxylic acid and spinosad. The bactericidal composition is used for preventing and treating soil-borne diseases, the use amount of phenazino-1-carboxylic acid is reduced, meanwhile, the prevention and treatment effect on crop diseases is not reduced or even improved, meanwhile, crop pests are effectively prevented, and the purposes of reducing cost and improving efficiency are achieved.

The utility model discloses a pesticide application device for preventing and treating vegetable soil-borne diseases, which relates to the technical field of agricultural machinery and comprises two rigid pesticide barrels, connecting plates are fixedly connected between two ends of the two rigid pesticide barrels, adding ports are arranged at the upper ends of the rigid pesticide barrels, two ends of the rigid pesticide barrel on one side are fixedly connected with wheel carriers, and the wheel carriers are fixedly connected with the connecting plates. A connecting frame is fixedly connected to the middle of the rigid pesticide barrel on the other side, a wheel shaft is rotationally connected between the two wheel frames, and driving wheels are installed at the two ends of the wheel shaft. Power can be provided for the driving wheels through the arranged driving assembly, a worker only needs to hold the pull handle to guide the direction in the using process, the labor intensity of a user is greatly reduced, meanwhile, due to the fact that the rigid pesticide barrel does not need to be carried by manpower, a larger rigid pesticide barrel can be designed, the pesticide carrying amount is greatly increased, and the labor intensity of the worker is reduced. Meanwhile, through cooperation with the second pesticide spraying pipe, lasting large-range pesticide spraying work can be achieved, and then the pesticide spraying efficiency is greatly improved.

The present application provides a kind of compound biocontrol agent and its preparation method and application technology, and it is composed of 1000ml of fermentation mixed liquor of multiple strains of biocontrol bacteria mixed in equal volume ratio and 0.5%-1.5% stabilizer with complementary mechanism and target.The fermentation concentrated solution of multiple strains of bacteria is prepared in fermentation medium to obtain the present application, which is used for preventing and controlling fusarium, pythium, phytophthora, rhizoctonia solani, ralstonia solanacearum, erwinia carotovora, streptomyces scabiei, xanthomonas campestris and other plant pathogenic bacteria and nematodes, which can inhabit in soil for a long time and spread through soil, and one or several of them can cause plant soil-borne diseases.The preparation process of the present application is not affected by too many chemical and physical factors, the activity of live bacteria and metabolites is high, the average control effect is 78%-85%, the application effect is good;The production process is simple, energy and auxiliary materials are saved, and the production cost is low.

The invention discloses streptomyces alboflavus (with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35588) for preventing and treating muskmelon fusarium wilt, and belongs to the field of biological prevention and treatment. The strain is high in sporulation quantity and strong in colonization capacity, pathogenic bacteria are inhibited and growth is promoted through antagonism, secondary metabolites, induction system resistance and other mechanisms, the potting control effect reaches 86.3%, and the effect lasts for 21 days. The streptomyces albidoflavus not only can prevent and treat fusarium wilt of muskmelons, but also has a broad-spectrum inhibition effect on fusarium wilt of cucumbers, watermelons and tomatoes and soil-borne diseases such as pepper phytophthora blight and rhizoctonia solani, and can improve rhizosphere microecology and promote reproduction of effective microbial communities. The biocontrol microbial agent is a pure biological agent, is pollution-free and nuisanceless, and can remarkably reduce the use of chemical pesticides, relieve continuous cropping obstacles and promote green development of the watermelon and melon industry.

The invention provides paenibacillus kuchii as well as a fungicide and application thereof, and relates to the field of microorganisms. The paenibacillus kuchii is specifically paenibacillus kuchii M18, and the preservation number of the paenibacillus kuchii M18 is CGMCC (China General Microbiological Culture Collection Center) No. 36293. The strain can be used for dissolving phosphorus and potassium, fixing nitrogen and secreting IAA (Indoleacetic Acid), and is beneficial to promoting plant growth and improving soil quality; the strain can produce a biological membrane, can effectively isolate the contact between a root system and pathogenic bacteria, and plays a crucial role in preventing and treating various soil-borne diseases; the strain can produce protease and cellulase and promote the growth of plants; the strain can produce a urease inhibitor, inhibit the activity of urease in soil and delay hydrolysis of urea. The strain also shows extremely strong salt tolerance, and is beneficial to exerting a growth promoting effect in saline-alkali soil. The microbial inoculum can be applied to the root of a plant by means of root irrigation, so that the strain is colonized at the root of the plant to play a role in promoting the growth of the plant.

The present application belongs to the field of microorganisms and biological control technology, and particularly relates to Burkholderia cenocepacia Tzs39 and application thereof in preventing and treating plant pathogenic fungi. The present application provides a Burkholderia cenocepacia Tzs39, and the preservation number of the Burkholderia cenocepacia Tzs39 is CGMCC No.30082. The antagonistic bacteria Tzs39 in the present application is isolated from rhizosphere soil of continuously cultivated Radix Pseudoginseng, has a good rhizosphere colonization effect and is safe and reliable, and can be used for preventing and treating or alleviating soil-borne disease problems of continuously cultivated medicinal plants, preventing and treating root rot in the process of continuously cultivating medicinal plants. The results of the embodiments show that the antagonistic bacteria Tzs39 can effectively inhibit the growth of Fusarium oxysporum and Trichoderma.

The present invention relates to the technical field of organic fertilizers, and discloses an organic water-soluble fertilizer taking wood vinegar liquid as a raw material and a preparation method thereof. The organic water-soluble fertilizer is made from the following raw materials in parts by weight: 35 - 45 parts of purified wood vinegar liquid, 15 - 20 parts of potassium humate, 8 - 12 parts of compound amino acids, 5 - 8 parts of chelated medium and trace elements, 2 - 3 parts of synergist, 0.5 - 1 part of pH regulator, and 11 - 34.5 parts of deionized water. The purified wood vinegar liquid is obtained by subjecting the crude wood vinegar liquid of a biomass boiler to pretreatment, gradient distillation, and adsorption and impurity removal, wherein the acetic acid content is ≥12%, the tar content is ≤0.1%, the pH value is 3.5 - 4.0, and the density is 1.12 - 1.15 g / cm3; the compound amino acids are compounded from glycine, glutamic acid, and proline according to a mass ratio of 2:1:1, and the total amino acid content is ≥95%. The present invention not only solves the toxicity problem of direct application of wood vinegar liquid but also increases the concentration of effective components. At the same time, a small amount of phenolic substances retained during the distillation process and the chitosan oligosaccharide in the synergist act synergistically, increasing the control effect of the fertilizer on soil-borne diseases to more than 85%.

This invention discloses a method for propagating superior ginger varieties using soilless cultivation, belonging to the field of ginger variety propagation technology. This invention provides a complete system of superior variety propagation technology. The method includes using a cultivation container with a tray at the bottom; filling it with a compound substrate; selecting healthy ginger seeds, germinating them, sowing them, covering them with soil and a green film; employing integrated water and fertilizer management, with a nitrogen-phosphorus-potassium ratio of 2:1:3 in the nutrient solution, and adjusting the EC value in stages; performing three hilling operations based on the number of branches, with a cumulative hilling height of 20cm; and controlling water and fertilizer in the later stages of growth to promote the maturation of the ginger seeds. This invention isolates soil-borne diseases through a soilless substrate, improves the quality of ginger seeds through precise water and fertilizer management and staged hilling, controls the seed usage per plant to around 100g, significantly reduces seed costs and disease incidence, establishes a standardized ginger variety propagation process, and produces ginger seeds that are uniform in size, have full buds, and exhibit excellent characteristics.

The application belongs to the technical field of agricultural microorganisms, and particularly relates to a plant endophytic Paenibacillus polymyxa and application thereof in preventing and treating plant diseases. Paenibacillus polymyxa The plant endophytic Paenibacillus polymyxa has the strain number PP8, the classification name Paenibacillus polymyxa, the preservation number CGMCC No. 36638, and is preserved in the China General Microbiological Culture Collection Center on November 17, 2025. The Paenibacillus polymyxa has broad-spectrum inhibitory activity on pathogenic fungi, oomycetes and bacteria, and can produce significant inhibitory effect on plant pathogenic microorganisms through multiple action mechanisms. In addition, the Paenibacillus polymyxa PP8 has the ability to colonize in the internal tissue of plant roots, can exist in the plant body and play the disease-inhibiting effect, thereby further enhancing the prevention and control effect on soil-borne diseases and improving the persistence and stability of the biological control effect.

This invention belongs to the field of biotechnology. To improve the effectiveness of biological control of plant diseases, this invention discloses *Bacillus velezensis* ZF516 and its application in controlling various soil-borne diseases. The *Bacillus velezensis* strain number is ZF516, and its registration number at the China General Microbiological Culture Collection Center is CGMCC No. 30136. This *Bacillus velezensis* strain can prevent and / or treat tomato bacterial canker, tomato bacterial wilt, or cowpea root rot, or can be used to prepare products for the prevention and / or treatment of these diseases. This strain also exhibits good inhibitory effects against *Fusarium oxysporum*, *Diplostomum spp.*, *Rhizoctonia solani*, *Fusarium solanum*, *Corynebacterium multiflorum*, *Botrytis cinerea*, *Agrobacterium tumefaciens*, *Pectinobacter brasiliensis*, *Ralstonia solanacearum*, *Streptomyces spp.*, etc.

The invention belongs to the technical field of biocontrol microbial agents, and particularly relates to a soil-borne disease biocontrol microbial agent as well as a preparation method and application thereof. The soil-borne disease biocontrol microbial inoculum is prepared by compounding a bacillus velezensis fermentation broth and a brevibacillus beautiful fermentation broth according to a volume ratio of 1: (0.5-1.5). Wherein the fermentation liquor contains thalli. The bacillus velezensis is a YCH-H7 strain, the preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) No.33510, and the preservation date of the bacillus velezensis is February 11, 2025. The brevibacillus beautiful is an NF2 strain. The corrected death rate of second-instar larvae treated by the soil-borne disease biocontrol microbial inoculum for 48 hours is 98.85%, the nematode egg incubation inhibition rate is 88%, and the corrected death rate of ditylenchus destructor treated for 4 days is 97.7%. The hypha growth inhibition rates of the soil-borne disease biocontrol microbial inoculum on kiwi fruit root rot pathogenic bacteria, pepper phytophthora blight pathogenic bacteria, tomato neck and root rot, cucumber root rot pathogenic bacteria and wheat stem rot pathogenic bacteria exceed 90%, and the soil-borne disease biocontrol microbial inoculum has obvious broad spectrum.