Prussella adamsii FJ08 strain, microbial inoculum, and preparation method and application of Prussella adamsii FJ08 strain and microbial inoculum

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a P.adami FJ08 strain, a fungicide, a preparation method and application thereof, which can be used for inhibiting soil-borne diseases of radix pseudostellariae. The invention discloses a P.aryabhattai FJ08, the P.aryabhattai FJ08 is preserved in the Microbial Culture Collection Center of Guangdong Province, the preservation number is GDMCC No: 65907, and the preservation date is March 24, 2025. The P.aryabhattai FJ08 has the advantages that the P.aryabhattai FJ08 can be used for preparing the P.aryabhattai FJ08; the P.aryabhattai FJ08 is separated from the radix pseudostellariae seed ginseng, is safe and reliable, and has the capabilities of inhibiting the growth of fusarium oxysporum, producing IAA, producing ammonia, dissolving potassium and dissolving phosphorus, and the growth of pathogenic fusarium oxysporum can be effectively inhibited by soaking the radix pseudostellariae seed ginseng with the P.aryabhattai FJ08.

Description

Technical Field

[0001] The present invention belongs to the field of microbial technology, and particularly relates to a Priestia aryabhattai strain FJ08, a microbial agent, and a preparation method and application thereof. Background Art

[0002] Pseudostellaria heterophylla, also known as Pseudostellaria heterophylla Miq. var. parvifolia (Miq.) Pax ex Pax & Hoffm. and Radix Pseudostellariae Heterophyllae, is the tuberous root of the perennial herb Heterostemma axillare (Roxb.) Wight of the Caryophyllaceae family. Pseudostellaria heterophylla was first recorded in "Bencao Congxin" and has now been included in the list of Chinese medicinal materials that can be used in health foods. Pseudostellaria heterophylla is a mild nourishing herb suitable for various symptoms such as spleen and lung deficiency, qi and yin deficiency, and qi and body fluid deficiency. Pseudostellaria heterophylla contains various chemical components such as trace elements, cyclic peptides, amino acids, sugars, glycosides, phospholipids, fatty acids, and sterols, and has good effects in improving myocardial ischemia, glycolipid metabolism in diabetes, and improving memory. Therefore, the artificial cultivation of Pseudostellaria heterophylla has good application prospects.

[0003] However, in the cultivation process of Pseudostellaria heterophylla, there are often serious soil-borne diseases, which in turn lead to a reduction in the yield of Pseudostellaria heterophylla and a decline in quality. Soil-borne diseases severely restrict the development of the Pseudostellaria heterophylla industry. Currently, chemical control methods are usually used to control soil-borne diseases of Pseudostellaria heterophylla, but the use of chemical drugs to control soil-borne diseases is likely to cause pesticide residues, which in turn affect the safety and effectiveness of the traditional Chinese medicine Pseudostellaria heterophylla. Therefore, how to provide a safe and pollution-free method that can effectively inhibit soil-borne diseases of Pseudostellaria heterophylla and inhibit continuous cropping obstacles of Pseudostellaria heterophylla has become an urgent problem to be solved in this field. Summary of the Invention

[0004] The purpose of the present invention is to provide a Priestia aryabhattai strain FJ08, a microbial agent, and a preparation method and application thereof. The Priestia aryabhattai FJ08 of the present invention can inhibit soil-borne diseases of Pseudostellaria heterophylla, inhibit continuous cropping obstacles of Pseudostellaria heterophylla, and promote the growth of Pseudostellaria heterophylla. The present invention specifically includes the following technical solutions.

[0005] A Priestia aryabhattai strain FJ08, which is preserved in the Guangdong Provincial Microbial Culture Collection Center with the preservation number GDMCC No: 65907 and the preservation date of March 24, 2025.

[0006] The present invention also provides a microbial agent, and the active ingredient of the microbial agent includes the above-mentioned Priestia aryabhattai strain FJ08.

[0007] Preferably, the viable count of Priestia aryabhattai strain FJ08 in the microbial agent is ≥ 4×10 8 CFU / mL.

[0008] The present invention also provides the application of Priestia aryabhattai FJ08 or the microbial inoculum as described above in inhibiting soil-borne diseases of medicinal plants and / or promoting the growth of crops.

[0009] Preferably, the promoting of crop growth includes promoting the seed germination of crops and / or promoting the growth of crop seeds.

[0010] Preferably, the plants include medicinal plants.

[0011] The present invention also provides a method for promoting plant growth, which is to treat plant seeds with the microbial inoculum as described above.

[0012] Preferably, the treatment method includes soaking the radix pseudostellariae with the microbial inoculum.

[0013] Preferably, the soaking time is 30 - 60 min.

[0014] Preferably, the plant seeds include the radix pseudostellariae seeds.

[0015] Beneficial effects:

[0016] The present invention provides a strain of Priestia aryabhattai FJ08, which is preserved in the Guangdong Provincial Microbial Culture Collection Center with the preservation number GDMCC No: 65907 and the preservation date of March 24, 2025. The Priestia aryabhattai FJ08 of the present invention is isolated from the radix pseudostellariae seeds, is safe and reliable, has the ability to produce IAA, produce ammonia, dissolve potassium, and dissolve phosphorus. Soaking the radix pseudostellariae seeds with Priestia aryabhattai FJ08 can effectively inhibit the growth of Fusarium oxysporum. Description of the drawings

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required in the embodiments.

[0018] Figure 1 It is a schematic diagram of the results of the plate confrontation experiment in Example 2;

[0019] Figure 2 It is the Gram staining result of Priestia aryabhattai FJ08 in Example 2;

[0020] Figure 3 It is the starch hydrolysis test result of Priestia aryabhattai FJ08 in Example 2;

[0021] Figure 4 It is the nitrate reduction test result of Priestia aryabhattai FJ08 in Example 2;

[0022] Among them, the left side is the control group and the right side is the experimental group;

[0023] Figure 5 It is the test result of acetylmethyl carbinol production by Priestia aryabhattai FJ08 in Example 2;

[0024] Among them, the left side is the control group and the right side is the experimental group;

[0025] Figure 6 It is the test result of ammonia production by Priestia aryabhattai FJ08 in Example 2;

[0026] Among them, the left side is the control group and the right side is the experimental group;

[0027] Figure 7 It is the test result of potassium dissolution by Priestia aryabhattai FJ08 in Example 2;

[0028] Among them, K467 shown in the figure is a mark made in the experiment and has no special meaning;

[0029] Figure 8 It is the experimental result of Fusarium oxysporum infecting Pseudostellaria heterophylla in Example 5;

[0030] Among them, the left side is the control group and the right side is the experimental group.

[0031] Biological deposit description

[0032] Priestia aryabhattai FJ08, with the biological classification name of Priestia aryabhattai FJ08, was deposited in the Guangdong Provincial Culture Collection of Microorganisms on March 24, 2025. The deposit number is: GDMCC No: 65907, and the deposit address is 100 meters due west of No. 2, 102 Xianlie Middle Road, Yuexiu District, Guangzhou. Detailed implementation manners

[0033] The present invention provides Priestia aryabhattai FJ08, which is deposited in the Guangdong Provincial Culture Collection of Microorganisms. The deposit location is: 100 meters due west of No. 2, 102 Xianlie Middle Road, Yuexiu District, Guangzhou. The deposit number is GDMCC No: 65907, and the deposit date is March 24, 2025. The characteristics of Priestia aryabhattai FJ08 on the LB solid medium are: flat and moist colonies, and the colonies are round and light yellow. Priestia aryabhattai FJ08 is a Gram-positive bacterium and has the abilities of hydrolyzing starch, reducing nitrate, decomposing glucose, producing ammonia, and dissolving potassium.

[0034] The present invention also provides a microbial inoculum, which includes Priestia aryabhattai FJ08 as described above.

[0035] As an implementation mode, the viable count of Priestia aryabhattai FJ08 in the microbial inoculant of the present invention is ≥ 4×10 8 CFU / mL. As another implementation mode, the viable count of Priestia aryabhattai FJ08 in the microbial inoculant of the present invention can be 4×10 8 CFU / mL, 5×10 8 CFU / mL, 6×10 8 CFU / mL, 7×10 8 CFU / mL, 8×10 8 CFU / mL, and 9×10 8 CFU / mL, any one of them. In a specific embodiment, the viable count of Priestia aryabhattai FJ08 in the microbial inoculant of the present invention is 4×10 8 CFU / mL.

[0036] The present invention also provides a preparation method of the microbial inoculant as described above, including the following steps:

[0037] Inoculate Priestia aryabhattai FJ08 into an LB liquid medium, and culture until the OD of the content of the LB liquid medium 600 value is 0.5 - 0.6 to obtain a bacterial liquid; perform the first centrifugation on the bacterial liquid, discard the supernatant, add sterile water, centrifuge again, discard the supernatant, and obtain a precipitate; add sterile water to the precipitate and mix to obtain the microbial inoculant.

[0038] The present invention inoculates Priestia aryabhattai FJ08 into an LB liquid medium, and cultures until the OD of the content of the LB liquid medium 600 value is 0.5 - 0.6 to obtain a bacterial liquid. As an implementation mode, the OD of the content of the LB liquid medium 600 value can be any one of 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, and 0.6.

[0039] After obtaining the bacterial liquid, the present invention centrifuges the bacterial liquid for the first time, removes the supernatant, adds sterile water, centrifuges again, removes the supernatant, and obtains a precipitate. As an embodiment, the specific operations of the first centrifugation and the re - centrifugation in the present invention are both centrifuging at 1000 rpm for 3 - 7 min. As another embodiment, the duration of the first centrifugation and the re - centrifugation in the present invention can be any one of 3 min, 4 min, 5 min, 6 min, and 7 min. As an embodiment, the amount of sterile water added can be 0.5 - 1.5 mL. As another embodiment, the amount of sterile water added can be any one of 0.5 mL, 0.6 mL, 0.7 mL, 0.8 mL, 0.9 mL, 1.0 mL, 1.1 mL, 1.2 mL, 1.3 mL, 1.4 mL, and 1.5 mL. The present invention centrifuges the bacterial liquid at 1000 rpm for 5 min for the first centrifugation, removes the supernatant, adds 1 mL of sterile water, centrifuges again at 1000 rpm for 5 min, removes the supernatant, and obtains a precipitate.

[0040] After obtaining the precipitate, the present invention adds sterile water to the precipitate and mixes to obtain the microbial inoculant. As an embodiment, the precipitate is mixed with sterile water until the effective concentration of Priestia aryabhattai FJ08 in the microbial inoculant ≥ 4×10 8 CFU / mL. As another embodiment, the effective concentration of the strains in the microbial inoculant of the present invention can be 4×10 8 CFU / mL, 5×10 8 CFU / mL, 6×10 8 CFU / mL, 7×10 8 CFU / mL, 8×10 8 CFU / mL, and 9×10 8 CFU / mL. In a specific embodiment, the effective concentration of the strains in the microbial inoculant of the present invention is 4×10 8 CFU / mL.

[0041] The present invention also provides the application of the Priestia aryabhattai FJ08 strain, the microbial inoculant as described above, or the microbial inoculant prepared by the preparation method in any one or several of inhibiting crop soil - borne diseases, inhibiting crop continuous cropping obstacles, and promoting crop growth.

[0042] As an embodiment, the crops include medicinal crops; the medicinal crops include Pseudostellaria heterophylla. As an embodiment, the crop soil - borne diseases include soil - borne diseases caused by Fusarium oxysporum.

[0043] The present invention also provides a method for inhibiting soil-borne diseases of crops, inhibiting continuous cropping obstacles of crops and / or promoting the growth of crops, which uses the microbial inoculant described above to soak crop seeds. As an implementation manner, the soaking duration is 30-60 min. As another implementation manner, the soaking duration can be any one of 30 min, 35 min, 40 min, 45 min, 50 min, 55 min and 60 min.

[0044] To further illustrate the present invention, the following will describe in detail a Priestia aryabhattai strain FJ08, an inoculant thereof, and a preparation method and application thereof provided by the present invention with reference to the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0045] Example 1 Isolation, screening and identification of Priestia aryabhattai FJ08

[0046] In this example, Pseudostellaria heterophylla seeds were collected from the Pseudostellaria heterophylla planting area in Zhejiang County, Fujian Province. 5 g of the seeds were weighed and placed in a conical flask, 45 mL of sterile water was added, and the mixture was shaken well in a constant temperature shaker for 30 min to obtain a 10 -1 dilute solution.

[0047] The conical flask containing the 10 -1 dilute solution was placed in a 75 °C water bath for 30 min. 5 mL of the 10 -1 dilute solution was transferred to another conical flask containing 45 mL of sterile water to obtain a 10 -2 dilute solution. According to the above operation, 10 -3 dilute solution and 10 -4 dilute solution were prepared in turn.

[0048] 50 μL of dilute solutions with different concentrations were respectively and evenly spread on the LB medium, and then placed in an incubator at 37 °C for 12 h. After the culture was completed, the growth of microorganisms at each dilution was observed, and the 10 -2 dilute solution with the best microbial growth was selected as the optimal dilution. All single colonies on this plate were picked and transferred to a new plate for continued culture. The culture time was about 10 h. When the colonies were clearly visible, the isolated and screened bacteria were obtained and stored in a 4 °C refrigerator for short-term use.

[0049] The isolated and screened bacteria were molecularly identified by using the 16s-23s rRNA gene interval PCR amplification technique. The primer sequences are shown in SEQ ID NO:1 and SEQ ID NO:2, and the sequencing sequence is shown in SEQ ID NO:3:

[0050] 27F (SEQ ID NO:1): 5′-AGAGTTTGATCCTGGCTCAG-3′;

[0051] 1492R (SEQ ID NO:2): 5'-TACGACTTAACCCCAATCGC-3';

[0052] SEQ ID NO:3:

[0053]

[0054] The PCR reaction system (25 μl) is as follows: 1 μl of each of the upstream and downstream primers, 2 μl of the template, 8.5 μl of enzyme-free water, and 12.5 μl of mix.

[0055] The PCR reaction program is denaturation at 94 °C for 1 min, annealing at 55 °C for 45 s, extension at 72 °C for 90 s, for 35 cycles. The sequenced sequences were compared and analyzed with the NCBI database using the BLAST tool.

[0056] Through molecular biological identification, this bacterium was determined to be Priestia aryabhattai, named Priestia aryabhattai FJ08. The isolated strain was deposited in the Guangdong Microbial Culture Collection Center on March 24, 2025, with the deposit number GDMCC No: 65907.

[0057] Observation of the Priestia aryabhattai FJ08 showed that the Priestia aryabhattai FJ08 formed flat and moist colonies on the LB solid medium, and the colonies had a metallic luster.

[0058] Example 2 Physiological and biochemical characteristics of Priestia aryabhattai FJ08

[0059] The screened Priestia aryabhattai FJ08 was transferred to an LB liquid medium for scale-up culture to obtain a Priestia aryabhattai FJ08 culture solution, and the Gram staining test, starch hydrolysis test, nitrate reduction test, acetoin production test, and ammonia production test were carried out in sequence.

[0060] (1) Plate confrontation experiment

[0061] A sterilized white pipette tip was used to dip into the mycelium of the pathogenic Fusarium oxysporum (Fusarium oxysporum was disclosed in the article "Liu Y T, Zhang Y X, Wang X Y, et al. Pseudostellaria heterophylla cultivar mixtures driven changes in rhizosphere metabolites to suppress soil-borne Fusarium disease[J]. Agriculture Ecosystems & Environment, 2025, 380."), and it was spotted at the center of a petri dish filled with PDA medium and cultured in a 37 °C biochemical incubator for 10 h. A sterilized white pipette tip was used to dip into Burkholderia SJ08, and a 1-cm horizontal line was drawn at a distance of 1 cm on both sides of the center of the medium, and it was cultured in a 37 °C biochemical incubator for 24 h. The growth of Fusarium oxysporum and Burkholderia SJ08 was observed, and the results were as Figure 1, It can be seen that the Burkholderia SJ08 strain provided by the present invention can significantly inhibit the growth of pathogenic Fusarium hyphae.

[0062] (2) Gram staining test

[0063] Dip an inoculating loop into the culture solution of Priestia aryabhattai FJ08 and directly smear it on a glass slide. Pass the back of the glass slide with the specimen through the hottest part of the alcohol lamp flame at a pendulum speed 3 times to fix the bacteria on the glass slide. Drop several drops of crystal violet stain on the fixed bacterial smear and let it act at room temperature for 1 min. Then gently rinse it with running water and drop several drops of mordant iodine solution, let it act at room temperature for 1 min, rinse with running water. After rinsing, drop several drops of 95% alcohol, gently shake the glass slide for 5 s to make it decolorize evenly. Then hold the glass slide obliquely to let the removed dye flow away with the alcohol. Drop alcohol again until the flowing alcohol is colorless or slightly light purple, immediately rinse off the alcohol with running water, counterstain with safranin solution for 2 min, wash with water. After the specimen is stained, air-dry it and observe it under a microscope. The results are shown in Figure 2 . According to Figure 2 it can be known that Priestia aryabhattai FJ08 is purple, indicating that Priestia aryabhattai FJ08 is a Gram-positive bacterium.

[0064] (3) Starch hydrolysis test

[0065] Prepare the starch hydrolysis medium according to the components shown in Table 1.

[0066] Table 1 Preparation of starch hydrolysis medium

[0067]

[0068] Inoculate Priestia aryabhattai FJ08 into the starch hydrolysis medium and culture it at 37 °C for 24 h. Drop iodine solution onto the medium and make the iodine solution cover the entire medium. Observe the test phenomenon under light. The results are shown in Figure 3 . According to Figure 3 it can be known that a colorless transparent circle appears around Priestia aryabhattai FJ08, indicating that Priestia aryabhattai FJ08 has the ability to hydrolyze starch.

[0069] (4) Nitrate reduction test

[0070] Prepare Griessia reagent according to the components shown in Table 2.

[0071] Table 2 Preparation of Griessia reagent

[0072]

[0073] Inoculate Priestia aryabhattai FJ08 into nitrate reduction liquid medium as the experimental group, and at the same time prepare the uninoculated medium as the control group. Incubate the control group and the experimental group together in an incubator at 37 °C for 48 h. After the incubation, add 50 μl of Griess reagent I and II to the culture solutions of the experimental group and the control group respectively in sequence. The results are shown in Figure 4 , according to Figure 4 , it can be seen that there is no change in the culture solution of the control group, and the culture solution of the experimental group shows red, indicating that Priestia aryabhattai FJ08 can reduce nitrate to nitrite, ammonia and nitrogen.

[0074] (5) Acetoin production test

[0075] Inoculate Priestia aryabhattai FJ08 into glucose peptone medium as the experimental group, and at the same time prepare the uninoculated glucose peptone medium as the control group. Incubate the control group and the experimental group in an incubator at 37 °C for 4 days.

[0076] Take out the culture media of the control group and the experimental group after the incubation. Add an equal amount of 40% (w / v) KOH to the culture solutions in the culture media of the control group and the experimental group respectively, then add 0.5 mg of creatine and shake. Observe the phenomenon within 10 min. If bacteria can decompose glucose to produce pyruvate in glucose peptone water culture solution, pyruvate condenses, decarboxylates to form acetoin, and the latter is oxidized to diacetyl by oxygen in the air under strong alkaline conditions. Diacetyl reacts with the guanidine group in peptone to form a red compound, which is called V-P (+) reaction. The test results are shown in Figure 5 , according to Figure 5 , it can be seen that the color of the culture solution of the experimental group turns red, which is a positive reaction, indicating that Priestia aryabhattai FJ08 has the ability to decompose glucose.

[0077] (6) Ammonia production test

[0078] Prepare the ammonia production determination medium according to the components shown in Table 3.

[0079] Table 3 Preparation of ammonia production determination medium

[0080]

[0081] Inoculate Priestia aryabhattai FJ08 into the ammonia production determination medium, and at the same time prepare the ammonia production determination medium without inoculating bacteria as the control. Incubate the control group and the experimental group together in an incubator at 37 °C for 24 h. After the incubation, add 5 ml of sodium reagent to the culture media of the control group and the experimental group respectively, and shake gently. According to Figure 6 , it can be seen that the experimental group produces an orange-red precipitate, which is a positive reaction, indicating that Priestia aryabhattai FJ08 has the ability to produce ammonia.

[0082] (7) Potassium dissolution test

[0083] A potassium-dissolving medium was prepared according to the composition shown in Table 4.

[0084] Table 4 Preparation of potassium-dissolving medium

[0085]

[0086]

[0087] Priesteria axyri FJ08 was inoculated into potassium-dissolving medium and cultured in a 37°C incubator for 48 hours. Figure 7 ,according to Figure 7 It can be seen that a colorless transparent circle appears around Priesteria afroyi FJ08, indicating that Priesteria afroyi FJ08 has the ability to dissolve potassium.

[0088] Example 3 A method for preparing a Priesteria aeruginosa FJ08 bacterial solution

[0089] Priesteria axotii FJ08 was inoculated into 800 μL LB liquid and cultured for 18 h until OD 600 After the pH value was 0.5 to 0.6, a culture solution was obtained. The culture solution was centrifuged at 1000 rpm for 5 min, the supernatant was discarded, 1 mL of sterile water was added, and the mixture was shaken evenly. The mixture was centrifuged again for 5 min, and the supernatant was removed to wash away the residual LB. After the precipitate was obtained, 5 mL of sterile water was added to the precipitate and shaken evenly to prepare a bacterial solution. The concentration of Priesteria aegypti FJ08 in the bacterial solution was 4.0 × 10 8 cfu / mL.

[0090] Example 4 A method for inhibiting soil-borne diseases and continuous cropping obstacles of Pseudostellaria heterophylla and promoting the growth of Pseudostellaria heterophylla

[0091] Pretreatment of ginseng seeds: Wash the surface soil of Pseudo-Pseudo-Pseudo-Ginseng seeds with clean water, disinfect the seeds with 2% sodium hypochlorite solution for 1 minute, rinse with distilled water 5 times, then disinfect with 75% ethanol for 2 minutes, rinse with distilled water 3 times, and absorb the surface moisture with filter paper.

[0092] Soak the Pseudostellariae Pseudostellariae seeds in the bacterial solution of Priesteria afroides FJ08 as described in Example 2 for 1 hour.

[0093] Example 5 Verification of the antibacterial effect of Priesteria axyri FJ08

[0094] In this example, 12 Pseudo-Pseudo-Pseudo-Ginseng species were taken and divided into 4 groups on average.

[0095] Treatment 1: Take 3 Pseudostellaria heterophylla seeds, and immerse them simultaneously in the liquid of Priestia aryabhattai FJ08 prepared by the preparation method described in Example 3. After soaking for 60 min, take out the seeds.

[0096] Treatment 2 (CK group): Take 3 seeds and immerse them in sterile water as the CK control. After soaking for 60 min, take out the seeds.

[0097] Treatment 3: Take 3 Pseudostellaria heterophylla seeds, soak them in the SJ06 bacterial agent. After soaking for 60 min, take out the seeds. The preparation method of the SJ06 bacterial agent is the same as that in Example 3, except that the SJ06 strain is used for preparation. The SJ06 is another strain screened by the applicant from Pseudostellaria heterophylla seeds according to the method described in Example 1 in the previous experiment.

[0098] Treatment 4: Take 3 Pseudostellaria heterophylla seeds, soak them in the FJ11 bacterial agent. After soaking for 60 min, take out the seeds. The preparation method of the FJ11 bacterial agent is the same as that in Example 3, except that the FJ11 strain is used for preparation. The FJ11 is another strain screened by the applicant from Pseudostellaria heterophylla seeds according to the method described in Example 1 in the previous experiment.

[0099] Use a scalpel in the autoclave to make a 0.5-cm small cut on the surface of the seeds after soaking in the above different treatments, and dip a sterilized white pipette tip in Fusarium oxysporum mycelium and smear it on the wound surface. Place the seeds in a petri dish lined with sterilized filter paper, then add 4 mL of sterile water to the petri dish to keep the seeds moist. Place the petri dish in a biochemical incubator and culture at 37 °C, and observe the growth status of Fusarium oxysporum on the wound surface of the Pseudostellaria heterophylla seeds. The results are as Figure 8 shown. Statistically analyze the length of the Fusarium oxysporum-infected fungal blocks on the Pseudostellaria heterophylla at 24, 36, and 48 h after inoculating Fusarium oxysporum. The results are shown in Table 5.

[0100] Table 5 Length of Fusarium oxysporum fungal blocks at different times (cm)

[0101] Treatment group / Time (h) 24 36 48 Treatment 2 (CK) 0.5 0.8 1.23 Treatment 1 0.44 0.58 0.75 Treatment 3 0.48 0.73 1.05 Treatment 4 0.48 0.75 1.14

[0102] As Figure 8 can be seen from and Table 5, compared with the control group, after the seeds treated with Priestia aryabhattai FJ08 are infected by the pathogenic Fusarium oxysporum, the wound surface formed is smaller, and the length of the Fusarium oxysporum fungal block is smaller, indicating that this bacterium has a good antibacterial effect.

[0103] In summary, the present invention provides a strain of Priestia aryabhattai FJ08. The bacterial liquid prepared using the Priestia aryabhattai FJ08 of the present invention has a good inhibitory effect on the soil-borne diseases of Pseudostellaria heterophylla, can effectively inhibit the crop diseases caused by Fusarium oxysporum, can also inhibit the continuous cropping obstacles of crops, promote the growth of crops, and is safe and pollution-free, having good application prospects.

[0104] Although the above embodiments have made a detailed description of the present invention, they are only a part of the embodiments of the present invention, rather than all embodiments. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A Priestia aryabhattai FJ08, characterized in that, The Priestia aryabhattai FJ08 is preserved in the Guangdong Microbial Culture Collection Center, with the preservation number GDMCC No: 65907 and the preservation date of March 24, 2025.

2. A microbial inoculant, characterized in that, The active ingredient of the microbial inoculant includes the Priestia aryabhattai FJ08 described in claim 1.

3. The microbial inoculant according to claim 2, characterized in that, The viable count of Priestia aryabhattai FJ08 in the microbial inoculum ≥ 4×10 8 CFU / mL.

4. Use of the Priestia aryabhattai FJ08 described in claim 1 or the microbial inoculant described in claim 2 or 3 in inhibiting soil-borne diseases of medicinal plants and / or promoting crop growth.

5. The application according to claim 4, wherein The promotion of crop growth includes promoting the seed germination of crops and / or promoting the growth of crop seeds.

6. The application according to claim 4 or 5, characterized in that, The plants include medicinal plants.

7. A method for promoting plant growth, characterized in that, Treat plant seeds with the microbial inoculant described in any one of claims 2 to 3.

8. The method according to claim 7, characterized in that, The treatment method includes soaking the ginseng seeds with the microbial inoculant.

9. The method according to claim 8, wherein The soaking time is 30 to 60 minutes.

10. The method according to any one of claims 7 to 9, characterized in that The plant seeds include Pseudostellaria heterophylla ginseng seeds.

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