Microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening and preparation method of microbial agent type bio-organic fertilizer

Abstract

The invention provides a microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening and a preparation method of the microbial agent type bio-organic fertilizer, and belongs to the technical field of organic fertilizers. The microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening comprises the following raw materials: an organic material, a microbial agent, an inorganic nutritional ingredient, vinasse and an additive, wherein the organic material is composed of livestock and poultry manure, crop straw, tobacco waste and a fermentation inoculant; the additive is prepared from humic acid, seaweed extract and amino acid. The organic fertilizer contains rich organic materials and humic acid, the content of organic matters in soil can be increased, the granular structure of the soil can be improved, the growth of soil-borne pathogenic bacteria can be inhibited by the added multiple beneficial microorganisms, the micro-ecological environment of the soil can be improved by the photosynthetic bacteria, and the incidence rate of diseases such as tobacco black shank and bacterial wilt can be reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of organic fertilizer, in particular to a microbial agent type bio-organic fertilizer capable of improving the hardening of continuous cropping tobacco soil and a preparation method thereof. BACKGROUND

[0002] QianDongNan state has unique geographical environment and climate conditions, and is an important planting area of high-quality tobacco. However, long-term continuous cropping mode makes the local tobacco planting soil nutrient imbalance, resulting in a series of adverse consequences such as tobacco yield reduction, quality deterioration, and pest intensification.

[0003] The main reasons for the above problems are:

[0004] (1) Tobacco has specific requirements for some nutrients in the soil (especially potassium, nitrogen, and phosphorus), and continuous cropping will continuously absorb these elements, while consuming trace elements in the soil (such as boron, zinc, and molybdenum), resulting in a significant decrease in the content of these key nutrients in the soil, and even if fertilization, it is difficult to completely restore the balance. And the absorption rate of different nutrients is different, and continuous cropping will lead to the imbalance of the ratio of various elements in the soil, affecting the absorption and utilization of tobacco to other elements.

[0005] (2) The pathogens (bacteria, fungi, nematodes, viruses) of many diseases of tobacco (such as bacterial wilt, black foot disease, root black rot, root knot nematode disease, tobacco mosaic disease, etc.) can survive in the soil or diseased residues for many years. Continuous cropping provides a stable host and breeding site for these pathogens, resulting in their accumulation in the soil, and the pathogenic base increases year by year. At the same time, continuous cropping destroys the diversity of soil microbial communities, resulting in a decrease in the number of beneficial microorganisms (such as antagonistic bacteria and mycorrhizal fungi), while harmful pathogenic microorganisms dominate, and the soil disease suppression ability decreases. When the accumulation of pathogenic bacteria reaches a certain level, it is easy to cause serious soil-borne diseases under suitable conditions (such as high temperature and humidity), causing devastating losses.

[0006] (3) Tobacco roots will secrete some organic substances (such as organic acids, phenols, alkaloids, etc.) during growth, and these substances or their degradation products will continuously accumulate in the soil under continuous cropping conditions. When these substances reach a certain concentration, they will have toxic effects on tobacco itself (especially seedling roots), inhibit seed germination, root growth and plant development, reduce stress resistance, and may also inhibit the activities of beneficial microorganisms in the soil, further deteriorating the soil micro-ecological environment.

[0007] (4) Long-term single tillage, mechanical compaction, and decline of organic matter lead to soil hardening; tobacco roots secrete H +Ion and the use of acidic fertilizer cause soil acidification; unreasonable application of chemical fertilizer causes soil salt accumulation, etc. are also the reasons for the problems of continuous cropping of tobacco soil. Acidification can increase the activity of toxic elements such as aluminum and manganese, inhibit the activity of beneficial microorganisms, and affect the availability of elements such as phosphorus, calcium and magnesium; salt accumulation can affect root water permeability, causing physiological drought and salt damage.

[0008] Soil compaction usually has problems such as high bulk density, low total porosity, and reduced number of large aggregates (diameter >0.25mm), resulting in reduced soil ventilation rate, insufficient oxygen content (usually less than 10%), slow water infiltration rate, easy surface runoff and water accumulation, and poor drainage.

[0009] Therefore, the continuous cropping of tobacco soil should be improved and health managed to restore and maintain the productivity and sustainability of tobacco planting soil. SUMMARY

[0010] Therefore, the purpose of the present application is to provide a microbial agent type of bio-organic fertilizer capable of improving the compaction of continuous cropping of tobacco soil, solving the problems of continuous cropping of tobacco soil such as decline of soil fertility, frequent occurrence of soil-borne diseases, and imbalance of microbial community, etc. to improve the yield and quality of tobacco.

[0011] In order to achieve the above purpose, the present application provides the following technical solutions.

[0012] A microbial agent type of bio-organic fertilizer capable of improving the compaction of continuous cropping of tobacco soil, comprising the following raw materials by weight:

[0013] Organic material 50-70 parts, microbial agent 6-12 parts, inorganic nutrient component 15-25 parts, vinasse 25-35 parts, additive 5-10 parts;

[0014] The organic material is composed of livestock and poultry manure, crop straw, tobacco waste and fermentation agent, wherein the mass ratio of livestock and poultry manure, crop straw and tobacco waste is 30-40:15-25:3-10;

[0015] The additive is composed of humic acid, seaweed extract and amino acid in a mass ratio of 3-6:1-3:1-2.

[0016] Preferably, the fermentation agent is at least one of yeast and lactic acid bacteria; the addition amount of the fermentation agent is 0.1-3% of the total mass of the organic material.

[0017] Preferably, the microbial agent is composed of Bacillus megaterium, Paenibacillus mucilaginosus, Trichoderma harzianum, photosynthetic bacteria and Bacillus licheniformis in a mass ratio of 2-3:2-3:1-2:1-2:1-2.

[0018] Preferably, the inorganic nutrient component is composed of nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer and trace element fertilizer in a mass ratio of 5-8:4-7:4-7:2-3.

[0019] More preferably, the trace element fertilizer is at least one of boron fertilizer, zinc fertilizer and manganese fertilizer.

[0020] The object of the present application is to provide a preparation method of microbial agent type bio-organic fertilizer capable of improving the soil compaction of continuous tobacco planting, comprising the following steps:

[0021] (1) Organic material pretreatment: mixing livestock and poultry manure, crop straw and tobacco waste in proportion, adjusting the moisture content to 50%-60% to obtain organic mixture;

[0022] (2) Composting fermentation: adding fermentation agent to the organic mixture in proportion, stirring uniformly, and then performing composting fermentation until the organic material is fully composted;

[0023] (3) Microbial agent preparation: activating and culturing Bacillus megaterium, Paenibacillus mucilaginosus, Trichoderma harzianum, photosynthetic bacteria and Bacillus licheniformis respectively, then expanding the culture in a suitable culture medium, and then mixing in proportion to prepare a microbial agent;

[0024] (4) Mixing and granulation: mixing the fermented organic material, microbial agent, inorganic nutrient component, vinasse and additive in proportion, stirring uniformly to obtain bio-organic fertilizer mixture, and then granulating by a granulator;

[0025] (5) Drying: drying the granules at low temperature to obtain microbial agent type bio-organic fertilizer capable of improving the soil compaction of continuous tobacco planting.

[0026] Preferably, in the composting fermentation process of step (2), the pile is turned over every 3-5 days, the pile temperature is controlled at 50-70℃, and the fermentation time is 20-30 days.

[0027] Preferably, the expansion culture in step (3) is to make the concentration of the bacterial solution reach 10 8 -10 10 CFU / mL.

[0028] Preferably, in the granulation process of step (4), a binder is added, the mass of the bio-organic fertilizer mixture being 2-5%, and the binder being any one or both of starch and bentonite.

[0029] Preferably, the drying temperature in step (5) is 40-60℃, and the moisture content in the granules after drying is less than 15%.

[0030] Compared with the prior art, the present application has the following beneficial effects:

[0031] (1) Improving soil physical and chemical properties: The organic fertilizer of the present application contains rich organic matter and humic acid additives, which can increase the content of soil organic matter, improve the soil aggregate structure, and improve the soil aeration, water permeability and water and fertilizer retention capacity. At the same time, the organic acid and other substances produced during the decomposition of organic matter can adjust the soil pH, and improve the soil chemical properties.

[0032] (2) Regulating soil microbial community structure: The addition of various beneficial microorganisms can rapidly reproduce in the soil, form dominant flora, interact with the original microorganisms in the soil, inhibit the growth and reproduction of harmful microorganisms, and increase the number and species of beneficial microorganisms. Bacillus megaterium, Paenibacillus mucilaginosus and other microorganisms can improve the soil nutrient availability, Trichoderma harzianum, Bacillus licheniformis and other microorganisms can inhibit the growth of soil-borne pathogens, and photosynthetic bacteria can improve the soil microecological environment.

[0033] (3) Enhancing soil fertility: Inorganic nutrient components combined with organic matter and microbial agents provide comprehensive and sustainable nutrient supply for tobacco growth. The nutrients in organic matter are released slowly, inorganic nutrient components can quickly supplement the nutrients required for tobacco growth, and microbial agents can improve the availability of soil nutrients. The synergistic effect of the three improves soil fertility and meets the nutrient needs of tobacco at different growth stages.

[0034] (4) Reducing the occurrence of soil-borne diseases: Trichoderma harzianum, Bacillus licheniformis and other beneficial microorganisms have significant inhibitory effect on tobacco soil-borne pathogens, which can reduce the incidence of tobacco black shank disease, bacterial wilt and other diseases, reduce the use of pesticides, and improve the health level of tobacco.

[0035] (5) Improving the yield and quality of tobacco: By improving the soil environment and providing sufficient nutrients, the growth and development of tobacco roots are promoted, the stress resistance of tobacco is enhanced, the occurrence of soil-borne diseases is reduced, and the yield of tobacco is improved. At the same time, tobacco absorbs more comprehensive and balanced nutrients, and the internal quality of tobacco leaf is improved, such as aroma, color, oil content and other indicators. DETAILED DESCRIPTION

[0036] The present application provides a microbial agent type biological organic fertilizer which can improve the hardening of continuous tobacco planting soil, comprising the following raw materials by weight:

[0037] Organic matter 50-70 parts, microbial agent 6-12 parts, inorganic nutrient component 15-25 parts, vinasse 25-35 parts, additive 5-10 parts;

[0038] The organic material is composed of livestock and poultry manure, crop straw, tobacco waste and fermentation agent, wherein the mass ratio of livestock and poultry manure, crop straw and tobacco waste is 30-40:15-25:3-10.

[0039] The additive is composed of humic acid, seaweed extract and amino acid in a mass ratio of 3-6:1-3:1-2.

[0040] The livestock and poultry manure is widely available, rich in various organic matter and nutrients, and can provide rich carbon and nitrogen sources for soil and improve soil structure after being fully decomposed.

[0041] In the present application, the crop straw contains a large amount of cellulose, hemicellulose and lignin, etc., and can increase the air permeability and water retention of soil after being treated.

[0042] In the present application, the tobacco waste (tobacco leaves, tobacco stems, tobacco dust, low-grade tobacco leaves, etc.) contains some special nutrients required for tobacco growth, which can improve the adaptability of tobacco to soil environment.

[0043] The humic acid in the additive can improve soil structure, increase soil aggregate structure, improve soil fertility and water retention capacity, and promote the activity of soil microorganisms; the seaweed extract contains various plant growth regulators and nutrient components, which can stimulate the growth of tobacco root system and enhance the stress resistance of tobacco; the amino acid can be directly absorbed and utilized by tobacco, participate in the synthesis of protein in tobacco, and improve the quality and yield of tobacco.

[0044] Preferably, the fermentation agent is at least one of yeast and lactic acid bacteria; and the addition amount of the fermentation agent is 0.1-3% of the mass of the organic material.

[0045] In the specific embodiment of the present application, the effective viable bacteria of the yeast in the fermentation agent is 1.5 billion; and the effective viable bacteria of the lactic acid bacteria is 1.5 billion.

[0046] Preferably, the microbial agent is composed of Bacillus megaterium, Paenibacillus mucilaginosus, Trichoderma harzianum, photosynthetic bacteria and Bacillus licheniformis in a mass ratio of 2-3:2-3:1-2:1-2:1-2.

[0047] In specific embodiments of the present application, the Bacillus megaterium (CGMCC 1.16094), Paenibacillus mucilaginosus (CGMCC 1.232), Trichoderma harzianum (CGMCC 5.1213), Bacillus licheniformis (CGMCC 1.10314) are purchased from China General Microbiological Culture Collection Center. The photosynthetic bacteria are purchased from Shandong Xinzhuoyuan Biotechnology Co., Ltd. with the item number 20230712.

[0048] Preferably, the inorganic nutrient component is composed of nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer and trace element fertilizer in a mass ratio of 5-8:4-7:4-7:2-3; the trace element fertilizer is at least one of boron fertilizer, zinc fertilizer and manganese fertilizer.

[0049] In specific embodiments of the present application, the nitrogen fertilizer is urea or ammonium sulfate, the phosphorus fertilizer is superphosphoric acid calcium or calcium-magnesium phosphate fertilizer, the potassium fertilizer is potassium sulfate or potassium chloride, the boron fertilizer is borax, the zinc fertilizer is zinc sulfate and the manganese fertilizer is manganese sulfate.

[0050] In the present application, the distiller's grains are common waste in the wine-making industry, rich in protein, polysaccharide, vitamins and various minerals, which can provide rich nutrients for soil microorganisms, promote the growth and reproduction of microorganisms, accelerate the decomposition and transformation of organic matter in the soil, and thus improve the soil structure.

[0051] The present application can improve the preparation method of microbial agent type bio-organic fertilizer for improving the soil compaction of continuous cropping tobacco planting, comprising the following steps:

[0052] (1) Organic material pretreatment: mixing livestock and poultry manure, crop straw and tobacco waste in proportion, adjusting the moisture content to 50%-60% to obtain organic mixture;

[0053] (2) Composting fermentation: adding fermentation agent to the organic mixture in proportion, stirring uniformly, and then performing composting fermentation until the organic material is fully composted; during the composting fermentation process, the pile is turned over every 3-5 days, the pile temperature is controlled at 50-70℃, and the fermentation time is 20-30 days;

[0054] (3) Microbial agent preparation: respectively activating and culturing Bacillus megaterium, Paenibacillus mucilaginosus, Trichoderma harzianum, photosynthetic bacteria and Bacillus licheniformis, then expanding the culture in a suitable culture medium for each to make the bacterial liquid concentration reach 10 8 -10 10 CFU / mL, then mixing in proportion to prepare a microbial agent;

[0055] (4) mixing and granulating: the fermented organic material, microbial inoculum, inorganic nutrient ingredient, vinasse and additive are mixed in proportion, fully stirred and uniformly obtained as a bio-organic fertilizer mixture, and then granulated by a granulator;

[0056] (5) drying: the granules are dried at 40-60℃ until the moisture content in the granules is below 15%, to obtain the microbial inoculum type bio-organic fertilizer capable of improving the soil compaction of continuous cropping tobacco soil.

[0057] Further, the preparation method of the microbial inoculum type bio-organic fertilizer capable of improving the soil compaction of continuous cropping tobacco soil, the specific method of step (2) compost fermentation is as follows:

[0058] Half of the fermentation inoculum is added to the organic mixture in proportion, stirred uniformly, and then composted and fermented, and the pile is turned over every 3-5 days, the pile temperature is controlled at 50-70℃, and the fermentation time is 20-30 days; after fermentation is completed, the remaining fermentation inoculum is added again for secondary fermentation, and the pile is turned over every 3-5 days during the secondary fermentation process, the pile temperature is controlled at 50-70℃, and the fermentation time is 15-30 days.

[0059] In the technical scheme of the present application, the secondary fermentation improves the degradation rate of nicotine and other components in tobacco waste.

[0060] Further, the preparation method of the microbial inoculum type bio-organic fertilizer capable of improving the soil compaction of continuous cropping tobacco soil, the specific method of step (3) mixing and granulating is as follows:

[0061] The fermented organic material and seaweed extract are mixed and aged for 7 days, and then mixed with microbial inoculum, inorganic nutrient ingredient and other additives in proportion, fully stirred and uniformly obtained as a bio-organic fertilizer mixture, and then granulated by a granulator.

[0062] In the technical scheme of the present application, the seaweed extract is mixed and aged with the fermented organic fertilizer, which improves the degradation rate of nicotine and other components in tobacco waste, and promotes the penetration and stability of active substances.

[0063] In the specific embodiments of the present application, 2-5wt% of the bio-organic fertilizer mixture is added as a binder during the granulation process in step (4), and the binder is any one or both of starch and bentonite.

[0064] The present application will be further described below in conjunction with examples. In the specific embodiments of the present application, the raw materials not specifically described are all purchased from the market.

[0065] Example 1

[0066] A microbial agent type bio-organic fertilizer capable of improving the compaction of continuous cropping tobacco soil, which is composed of the following raw materials in parts by weight:

[0067] Organic material: 35 parts of livestock and poultry manure, 20 parts of rape straw, and 5 parts of tobacco waste; the organic material further contains 0.5wt% of yeast and 0.3wt% of lactic acid bacteria;

[0068] Microbial agent: 2 parts of Bacillus megaterium, 2 parts of Bacillus mycoides, 1 part of Trichoderma harzianum, 1 part of photosynthetic bacteria, and 1 part of Bacillus licheniformis;

[0069] Inorganic nutrient component: 6 parts of urea, 5 parts of superphosphate, 5 parts of potassium sulfate, 1 part of borax, and 1 part of zinc sulfate;

[0070] Distiller's grains: 30 parts;

[0071] Additive: 4 parts of humic acid, 2 parts of seaweed extract, and 1 part of amino acid.

[0072] The preparation method of the microbial agent type bio-organic fertilizer capable of improving the compaction of continuous cropping tobacco soil, and the steps are as follows:

[0073] (1) Organic material pretreatment: mixing livestock and poultry manure, crop straw, and tobacco waste according to the proportion, adjusting the water content to 55%, and obtaining organic mixture;

[0074] (2) Composting fermentation: adding fermentation agent to the organic mixture according to the proportion, stirring uniformly, and then performing composting fermentation; during the composting fermentation process, the compost is turned over every 4 days, the temperature of the compost is controlled at 55-65℃, and the fermentation time is 25 days;

[0075] (3) Preparation of microbial agent: respectively activating and culturing Bacillus megaterium, Bacillus mycoides, Trichoderma harzianum, photosynthetic bacteria, and Bacillus licheniformis, then expanding the culture in a suitable culture medium respectively to make the concentration of the bacterial liquid reach 10 9 CFU / mL, then mixing according to the proportion to prepare the microbial agent;

[0076] (4) Mixing and granulation: mixing the fermented organic material, microbial agent, inorganic nutrient component, distiller's grains, and additive according to the proportion, fully stirring for 30 min to be uniform, obtaining bio-organic fertilizer mixture, then adding 2wt% of starch as a binder, and granulating through a granulator to obtain particles with a size of 3-4mm;

[0077] (5) Drying: drying the particles at 50℃ until the water content in the particles is 12%, obtaining the microbial agent type bio-organic fertilizer capable of improving the compaction of continuous cropping tobacco soil.

[0078] Example 2

[0079] A microbial inoculant type bio-organic fertilizer capable of improving the soil compaction of continuous tobacco planting, raw materials and preparation method are the same as example 1. The difference is that the preparation method of the microbial inoculant type bio-organic fertilizer capable of improving the soil compaction of continuous tobacco planting in example 2 is as follows:

[0080] (1)-(3): the same as example 1;

[0081] (4) mixed granulation: the fermented organic material and seaweed extract are mixed and aged for 7 days, then mixed with microbial inoculant, inorganic nutrient ingredients, vinasse and additives in proportion, fully stirred for 30 min to uniform, then 2wt% starch is added as a binder, and granulated by a granulator to obtain 3-4mm particles;

[0082] (5) the same as example 1.

[0083] Example 3

[0084] A microbial inoculant type bio-organic fertilizer capable of improving the soil compaction of continuous tobacco planting, raw materials and preparation method are the same as example 1. The difference is that the preparation method of the microbial inoculant type bio-organic fertilizer capable of improving the soil compaction of continuous tobacco planting in example 3 is as follows:

[0085] (1): the same as example 1;

[0086] (2) compost fermentation: half of the fermentation inoculant is added to the organic mixture in proportion, stirred uniformly, then composted and fermented, the compost is turned over every 4 days during the fermentation process, the temperature of the compost is controlled at 55-65℃, and the fermentation time is 20 days; after the fermentation is completed, the remaining fermentation inoculant is added for secondary fermentation, the compost is turned over every 4 days during the secondary fermentation process, the temperature of the compost is controlled at 55-65℃, and the fermentation time is 15 days;

[0087] (3)-(5): the same as example 1.

[0088] Example 4

[0089] A microbial inoculant type bio-organic fertilizer capable of improving the soil compaction of continuous tobacco planting, raw materials and preparation method are the same as example 1. The difference is that the preparation method of the microbial inoculant type bio-organic fertilizer capable of improving the soil compaction of continuous tobacco planting in example 4 is as follows:

[0090] (1)-(3): the same as example 3;

[0091] (4) mixing granulation: the fermented organic material and the seaweed extract are mixed and aged for 7 days, then mixed with the microbial inoculant, inorganic nutrient ingredients, vinasse and additives in proportion, fully stirred for 30 min to be uniform, to obtain a bio-organic fertilizer mixture, then 2wt% starch is added as a binder, and granulation is performed by a granulator to obtain particles of 3-4 mm;

[0092] (5) same as example 3.

[0093] Comparative example 1

[0094] A microbial inoculant type bio-organic fertilizer capable of improving the compaction of continuously cropped tobacco soil, the raw materials and preparation method are the same as example 1. The difference is that no tobacco waste is added in comparative example 1.

[0095] Comparative example 2

[0096] A microbial inoculant type bio-organic fertilizer capable of improving the compaction of continuously cropped tobacco soil, the raw materials and preparation method are the same as example 1. The difference is that no seaweed extract is added in comparative example 2.

[0097] Comparative example 3

[0098] A microbial inoculant type bio-organic fertilizer capable of improving the compaction of continuously cropped tobacco soil, the raw materials and preparation method are the same as example 1. The difference is that the amount of seaweed extract added in comparative example 3 is 5 parts.

[0099] Experiment

[0100] Field tests were conducted using the microbial inoculant type bio-organic fertilizers prepared in examples 1-4 and comparative examples 1-3.

[0101] Tobacco variety: Yunyan 100;

[0102] The specific treatment method is as follows:

[0103] A continuously cropped tobacco plot for three years was selected and divided into 8 groups for tobacco planting (marked as A, B, C, D, E, F, G, H groups), with 300 plants in each group, and each group was divided into three subplots, i.e. 100 plants in each subplot. The microbial inoculant type bio-organic fertilizers in the examples and comparative examples of the application were applied as base fertilizer, and the fertilization standard was 80 kg / mu for base fertilizer application, and topdressing was performed 45 days after transplanting, with 10 kg / mu of potassium nitrate as topdressing, and no further fertilizer was applied subsequently.

[0104] Among them, group A was used as a blank control group without applying microbial inoculant type bio-organic fertilizer, groups B, C, D and E applied microbial inoculant type bio-organic fertilizers of examples 1-4 of the application respectively, and groups F, G and H applied microbial inoculant type bio-organic fertilizers of comparative examples 1-3 of the application respectively.

[0105] Test different groups of soil physical properties, soil nutrient content, tobacco quality and yield.

[0106] After 50 days after transplanting, the soil and tobacco of each plot were determined, and the determination of tobacco was to select 15 representative tobacco plants for measurement.

[0107] (1) The soil bulk density (g·cm -3 ) of different groups is shown in Table 1.

[0108] Table 1

[0109]

[0110] (2) The influence of different treatments on the soil porosity (%) of the continuous cropping flue-cured tobacco is shown in Table 2.

[0111] Table 2

[0112]

[0113] (3) The influence of different treatments on the soil capillary porosity (%) of the continuous cropping flue-cured tobacco is shown in Table 3.

[0114] Table 3

[0115]

[0116] (4) The influence of different treatments on the soil aggregate (%) of the flue-cured tobacco at the maturation stage is recorded, and the content of the blank control group is taken as 1, and the change of other test groups with respect to the blank control group is shown in Table 4.

[0117] Table 4

[0118]

[0119] (5) The influence of different treatments on the soil water content (%) of the continuous cropping flue-cured tobacco is shown in Table 5.

[0120] Table 5

[0121]

[0122] From Tables 1 to 5, it can be seen that the microbial agent type biological organic fertilizer prepared in the application can significantly reduce the soil bulk density, improve the soil porosity and capillary porosity, increase the number of large aggregates in the soil, improve the soil water content, and effectively improve the soil hardening.

[0123] (6) The influence of different treatments on the soil alkali-hydrolyzable nitrogen content (mg N / kg) is shown in Table 6.

[0124] Table 6

[0125]

[0126] (7) The effects of different treatments on the content of available phosphorus in soil (mg / kg) are shown in Table 7.

[0127] Table 7

[0128]

[0129] (8) The effects of different treatments on the content of available potassium in soil (mg / kg) are shown in Table 8.

[0130] Table 8

[0131]

[0132] (9) The effects of different treatments on the content of organic matter in soil (g / kg) are shown in Table 9.

[0133] Table 9

[0134]

[0135] (10) The effects of different treatments on the appearance quality of upper leaves of flue-cured tobacco are shown in Table 10.

[0136] Table 10

[0137]

[0138] (11) The effects of different treatments on the physical properties of upper leaves of flue-cured tobacco are shown in Table 11.

[0139] Table 11

[0140]

[0141] (12) The effects of different treatments on the chemical components of upper leaves of flue-cured tobacco are shown in Table 12.

[0142] Table 12

[0143]

[0144] (13) The effects of different treatments on the yield and quality of flue-cured tobacco are shown in Table 13.

[0145] Table 13

[0146]

[0147] (14) The effects of different treatments on the black shank and bacterial wilt of flue-cured tobacco are shown in Table 14.

[0148] Table 14

[0149]

[0150] The above merely describes the preferred embodiments of the present application, and it should be pointed out that, for those skilled in the art, several improvements and refinements can be made without departing from the principles of the present application, and these improvements and refinements should also be considered as falling within the protection scope of the present application.

Claims

1. A microbial inoculant-based bio-organic fertilizer capable of improving soil compaction in continuously cropped tobacco plantations, characterized in that, Including the following parts by weight of raw materials: 50-70 parts organic materials, 6-12 parts microbial agents, 15-25 parts inorganic nutrients, 25-35 parts distiller's grains, and 5-10 parts additives; The organic material is composed of livestock and poultry manure, crop straw, tobacco waste and fermentation agent, wherein the mass ratio of livestock and poultry manure, crop straw and tobacco waste is 30-40:15-25:3-10; The additive is composed of humic acid, seaweed extract, and amino acids in a mass ratio of 3-6:1-3:1-2.

2. The microbial inoculant-based bio-organic fertilizer according to claim 1, which can improve soil compaction in continuous tobacco cropping, is characterized in that, The fermentation agent is at least one of yeast and lactic acid bacteria; the amount of fermentation agent added is 0.1-3% of the total mass of organic materials.

3. The microbial inoculant-based bio-organic fertilizer according to claim 1, characterized in that, The microbial agent is composed of Bacillus megaterium, Bacillus colloidis, Trichoderma harzianum, photosynthetic bacteria, and Bacillus licheniformis in a mass ratio of 2-3:2-3:1-2:1-2:1-2.

4. The microbial inoculant-based bio-organic fertilizer according to claim 1, characterized in that, The inorganic nutrients consist of nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer and trace element fertilizer in a mass ratio of 5-8:4-7:4-7:2-3.

5. The microbial inoculant-based bio-organic fertilizer according to claim 4, characterized in that, The micronutrient fertilizer is at least one of boron fertilizer, zinc fertilizer, and manganese fertilizer.

6. The method for preparing microbial inoculant-based bio-organic fertilizer capable of improving soil compaction in continuous tobacco cropping according to any one of claims 1-5, characterized in that, Includes the following steps: (1) Organic material pretreatment: Livestock and poultry manure, crop straw and tobacco waste are mixed in proportion and the moisture content is adjusted to 50%-60% to obtain organic mixture; (2) Composting fermentation: Add fermentation agent to the organic mixture in proportion, stir evenly, and then carry out composting fermentation until the organic material is fully decomposed; (3) Preparation of microbial agents: Bacillus megaterium, Bacillus colloidis, Trichoderma harzianum, photosynthetic bacteria and Bacillus licheniformis were activated and cultured respectively, and then expanded in their respective suitable culture media. They were then mixed in proportion to prepare microbial agents. (4) Mixing and granulation: The fermented organic materials, microbial agents, inorganic nutrients, lees and additives are mixed in proportion and stirred evenly to obtain a bio-organic fertilizer mixture, which is then granulated by a granulator. (5) Drying: The granules are dried at low temperature to obtain a microbial inoculant-type bio-organic fertilizer that can improve soil compaction in continuous tobacco planting.

7. The method for preparing microbial inoculant-based bio-organic fertilizer capable of improving soil compaction in continuous tobacco cropping according to claim 6, characterized in that, In step (2), the composting process is carried out by turning the pile over every 3-5 days, controlling the pile temperature at 50-70℃, and the fermentation time is 20-30 days.

8. The method for preparing microbial inoculant-based bio-organic fertilizer capable of improving soil compaction in continuous tobacco cropping according to claim 6, characterized in that, The scale-up culture described in step (3) is to bring the bacterial concentration to 10. 8 -10 10 CFU / mL.

9. The method for preparing microbial inoculant-based bio-organic fertilizer capable of improving soil compaction in continuous tobacco cropping according to claim 6, characterized in that, In step (4), 2-5% of the mass of the bio-organic fertilizer mixture is added as a binder during the granulation process. The binder is any one or two of starch and bentonite.

10. The method for preparing microbial inoculant-based bio-organic fertilizer capable of improving soil compaction in continuous tobacco cropping according to claim 6, characterized in that, The drying temperature in step (5) is 40-60℃, and the moisture content of the particles after drying is less than 15%.

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