33 results about "Quality culture" patented technology

The invention discloses a Chlorella autotrophic-heterotrophic mixed culture method, belonging to the technical field of biology. The method comprises the following steps: heterotrophic culture of Chlorella in mechanical-stirring fermentation tank, Chlorella solution dilution, and optical autotrophic culture of Chlorella. By using the method, the Chlorella can achieve high-density culture level in a short culture period, and the quality of Chlorella is greatly enhanced as compared with that of the Chlorella subjected to heterotrophic culture, thereby achieving the optical autotrophic culture level and implementing high-density high-quality culture of Chlorella.

The invention relates to a multifunctional menstrual blood stem cell culture method which comprises the following steps: menstrual blood and plasma acquisition, menstrual blood bactericidal cleaning, menstrual blood filtration, autoplasma preparation, single karyocyte separation, menstrual blood stem cell culture, menstrual blood menstruation purification and amplification, and menstrual blood stem cell cryopreservation. The method overcomes the defects caused by the required use of high-concentration fetal calf serum for culture in the prior art; and by using the own plasma of the customer to culture the cells, the customer can feel safe and acceptable, thereby implementing the safe, stable and quick high-quality culture system for amplifying the mesenchymal stem cells in deed.

The invention discloses a composition of sturgeon forage. The composition comprises yellow corn, soybean, fish meal, dried small shrimps, rice bran, yeast powder, bone meal, fish oil, algae, folic acid, lysine, calcium hydrogen phosphate, vitamin, salt, and trace elements. The plant protein originated from yellow corn almost displaces 40% of animal protein originated from fish meal in the forage formula, so the forage cost is largely reduced. The composite of sturgeon forage has the advantages of good ability in food calling, good growth state of sturgeon, strong stability in water and low death rate of grown sturgeons during transportation. Furthermore, sturgeons digest and absorb the forage with a high utilization rate, so sturgeons defecate less feces, the formation of high-quality culture environment is facilitated to a certain extent, and environmental pollution is reduced.

The invention discloses a school big data management platform. According to the technical key points, the platform comprises a data collection module, a data management module, a storage retrieval module, a real-time calculation module, a mining algorithm module, a scientific research practice module, a unified API module, a data operation and maintenance module, and a data security module; a cloud data center is formed, which is accessed and integrated with various background public services of connection, configuration, management, operation and maintenance, and a public basic support platform for supporting business applications of a transaction type, an information type, a service type, an interactive type and the like; and the public basic platform comprises a service capability framework, a platform capability framework and a function presentation framework. The school big data management platform has the beneficial effects that theories of comprehensive quality management, target management, knowledge management, excellent performance management and the like are realized; by taking assessment diagnosis as a starting point, and based on standard and system construction, an interior and exterior combination-based comprehensive and diversified quality guarantee mechanism is formed; and college quality culture taking self-discipline as a main feature is cultivated.

The invention provides a low-serum culture medium suitable for the growth of Vero cells. The dosage of calf serum for conventional culture is reduced to 2 percent from 10 percent (which is the contentof a component V), and conventionally added animal derived components are replaced with plant derived components and recombinant protein. According to the low-serum culture medium provided by the invention, the dosage of the serum is reduced, so that the cost is reduced, and the possibility that the serum in the culture medium carries exogenous pathogenic bacterium pollution is reduced; meanwhile, a lot of hybrid proteins in the serum are reduced and separation and purification of down-stream products are facilitated; plant hydrolyzed protein and recombinant endothelial growth factors are used for replacing the animal derived components so that the side effect of the animal derived components is reduced and the safety of biological products is improved. According to the culture medium provided by the invention, the Vero cells grow well and the cost is reduced; the exogenous pathogenic bacterium pollution is reduced, a post-period purification technology is reduced and the stability ofproducing cell products is improved; one good-quality culture medium is provided for producing the biological products which take the Vero cells as virus hosts or expression vectors.

The invention discloses an underwood wild-imitation standardized planting method for radix polygonati officinalis.An excellent species of radix polygonati officinalis is selected, planting is conducted in an appropriate period by selecting appropriate soil and appropriate artificial forest land type, an appropriate field management method is adopted, harvesting and seed reserving are conducted in good time, and appropriate primary processing and storing modes are selected.By means of the method, wild-imitation original-ecology high-yield and high-quality culture is conducted, the product is safe in quality, effective, stable and controllable, standardized large-area planting can be achieved, production cost is low, and benefits are high; the requirements of the Good Agricultural Practice for Chinese Crude Drug (on trail) formulated by State Food and Drug Administration are met; meanwhile, the land utilization rate and the land cropping index can be increased, and a good compound agriculture and forestry ecological system is established.

The invention provides a cephalotaxus hainanensis embryo culturing and seedling breeding method which relates to a plant quick propagating and breeding method. The method comprises the following steps: (1) collection and selection of explants; (2) cleaning and sterilization of seeds and embryo obtainment; (3) inoculation and culture of embryos; (4) passage and root induction of test-tube seedlings; and (6) acclimatization and transplantation. The invention firstly creates the whole method of using cephalotaxus hainanensis embryos as explants to perform tissue culture and quick propagation, andprovides high-quality culture mediums for breeding the cephalotaxus hainanensis embryos, thereby not only producing regular high-quality cephalotaxus hainanensis seedlings and providing a technical support for the quick propagation and restoration protection of rare or endangered species of cephalotaxus hainanensis and the development and the utilization of resources, but also laying foundation for further exploring gene engineering of cephalotaxus hainanensis, widening explant materials, and the like.

The invention discloses a method for high-yield and high-quality culture of lucid ganoderma with application of light and relates to the technical field of medical fungi culture. According to the invention, control with light of different wavelengths is applied during the whole culture course of lucid ganoderma, and LED lamps with different colors are used as light sources; or, different light qualities are obtained through placement of a filter coating on a culture object under a common light source or natural light. Through regulation and control of different light qualities, growth and development states of lucid ganoderma hyphae could be changed. On one side, growth speed of lucid ganoderma is increased. On the other side, the contents of effective constituents in lucid ganoderma (ganoderan and ganodenic acid) are increased. Hence, the growth cycle of lucid ganoderma is increased, and lucid ganoderma quality is improved. According to the invention, the method is simple; the cost is low; the lucid ganoderma cultured by the method has higher commodity values; and significant economic benefits can be obtained.

The invention belongs to the technical field of biological strain culture, and discloses a high quality culture method. The method comprises the following steps of (1) culture medium preparation, wherein corn cobs, cottonseed husks, bran, corn flour, rice bran and soybean hulls are stirred, then sawdust is added, stirring continues to be conducted, lime and calcium carbonate are uniformly scattered, water is added, stirring is conducted, the mixture is loaded into a culture bottle immediately after stirring is finished, and steam sterilization is conducted after bottling is finished; (2), inoculation, the sterilized culture bottle in step (1) is subjected to ultraviolet disinfection and ozone disinfection, after cooling is conducted, a hypsizygus marmoreus liquid strain is inoculated; (3),culture management, wherein culture is conducted in three stages, and mycelium stimulation treatment is conducted after culture; (4), fruiting management, wherein warehouse humidity and CO2 concentration in the inducement to primordium period, germination control period, incubation period and growing period are limited; (5), harvesting and storing, wherein harvesting and storing are conducted ina room at 15-18 DEG C, and products packed on the same day are stored in the warehouse. According to the culture method, the production cycle is shortened, and it is guaranteed that produced fresh hypsizygus marmoreus is high in quality and safe.

The invention discloses a production method of lignite wax resin activation modifying liquid, in particular to a processing technology and a new path. The processing technology is characterized in that lignite wax resin is subjected to activation modifying at high temperature and high pressure and is scientifically compounded with other nutritional elements. The new path is used for efficiently utilizing the lignite wax resin in a way of changing wastes into valuables. A prepared product has good effects in the fields of fish disease control, silkworm harvesting, high-quality culture of seedlings, output increase of crops, and the like; the market prospect is huge, the toxicity and environmental pollution are avoided, and the flexibility and convenience in use are realized. The production method has the advantages that the technological process is simple, the energy-saving and environment-friendly effects are realized, the production efficiency is high, the safety is realized, the operability is strong, the cost is relatively low, and the popularizing is easy.

The invention discloses a productive service public application quality quantification model method using quality factor step model constraint to realize Internet information product, goods and article quality culture appeal, and thus creating public application quality evaluate score accumulation and attainment credibility improving accumulation; the method uses accumulation savings quantification linearity step represented by the Chinese character Ping structure as quality appeal culture, and uses the Ping as a basic symbol: 1C (cao 1point), 1S (shui 10points) and 1P (ping 100 points) are employed, classified and clustered; the unification system can solve the quantification system troubles and problems that public quality appeal cannot be quantified, thus obtaining application quality appeal value converted public credibility culture; a production factor multi-appeal gather method: public attainment score (C.)+enterprise attainment score (S. enterprise QP)+public quality evaluate gather (P. public GP)=three-grade public data model (CPD).

The invention discloses feed for Monopterus albus. The feed comprises the following raw materials in parts by weight: 20-35 parts of wheat cakes, 3-20 parts of peanut vines, 30-50 parts of mulberry leaves, 10-15 parts of straw, 2-6 parts of masson pine leaf juice, 5-15 parts of cotton seed cakes, 10-20 parts of corn flour, 5-10 parts of fish bone meal, 1-6 parts of sapropel, 1-2 parts of salt, 2-6parts of microbial enzymes, 2-8 parts of tryptophan, 2-8 parts of vitamin A, and 1-5 parts of mineral substances. The feed for Monopterus albus is used, in order to optimize deficiency of original feed for Monopterus albus; a lot of green herbaceous plants are supplemented in the feed for Monopterus albus, in order to avoid the problem that green herbaceous plants needed by Monopterus albus cannot be normally supplied due to deficiency of green herbaceous plants in winter; the feed can promote uniform nutrition of Monopterus albus, so that Monopterus albus grows fast with good meat quality, and economic benefits of meat quality culture are improved.

The invention provides a microbial solid fermentation cultivation plant which is good in sealing, high in energy efficiency and low in cost. The microbial solid fermentation cultivation plant comprises a closed space defined by the ground, a top plate and four walls; a door is arranged on one wall, ventilation openings are formed in the other two or three walls, an air inlet is formed in the top plate, a high-efficiency filter is arranged at the air inlet, and a draught fan is arranged below the high-efficiency filter; air return louvers are arranged at the ventilation openings, steel wire window gauzes are arranged on the inner sides of the air return louvers, a plurality of layers of gauzes are fixed between the steel wire window gauzes and the air return louvers, and cotton is wrapped in the multiple layers of gauzes; and porous plates are arranged at the edge of the walls, and water accumulating cavities are arranged below the porous plates. By means of the microbial solid fermentation cultivation plant, the high temperature and high humidity environment for solid fermentation is kept, the energy efficiency is high, and cost is low; fermentation sundry bacterial contamination is less, the culture viable number and quality are greatly improved, and the yield and efficiency are greatly improved; and the high quality cultures can be produced.

The invention discloses Chinese herbal medicine saline water for prawn culture and a preparation method thereof. The Chinese herbal medicine saline water for prawn culture comprises the following rawmaterials in parts by mass: 10-35 parts of folium artemisiae argyi, 10-25 parts of fortune eupatorium herb, 20-40 parts of indigowoad root, 10-15 parts of indigowoad leaf, 10-15 parts of fructus forsythiae, 10-40 parts of an eucalyptus leaf extract and 30-70 parts of saline water with a mass concentration of 5%. The Chinese herbal medicine saline water provided by the invention can achieve effective sterilization and disinfection when used for prawn culture, can obviously enhance the disease resistance of prawns and realize high-yield and high-quality culture of the prawns, and has the characteristics of being free of toxicity and harm, simple in preparation method and low in cost.

The invention relates to the technical field of earthworm culture, in particular to a free selection system of earthworm ecological factors. The free selection system comprises a single test researchdevice and a group test research device which are connected in sequence, the single test research device is combined with the group test research device, the relationship between individual differences and group interaction is fully taken into consideration, and reasonable and scientific culture environment conditions are obtained, which is beneficial to the high quality culture of earthworms. Thesystem does not need time and effort and is simple in operation.

The invention discloses standard colorimetric paper for plant petals/leaves. The standard colorimetric paper comprises short fibers with the length less than or equal to 180 microns and long fibers with the length greater than or equal to 180 microns, and the widths of the long fibers and the short fibers are 4.2-6.5 microns; the compactness of the colorimetric paper is 0.618-0.632 g.cm<-3>, the tearing index of the colorimetric paper is 4.92-5.08 mN.m<2>.g<-1>. According to the standard colorimetric paper for the plant petals/leaves, the standard colorimetric paper is prepared from maize straw, the contrast accuracy is high, use is convenient, the area of a contrast can be estimated, and the prepared colorimetric paper has both high appearance quality and physical index to achieve the standard of high quality culture paper.