Chlorella autotrophic-heterotrophic mixed culture method

A mixed culture and heterotrophic culture technology, applied in the biological field, can solve the problem of high cost and achieve the effect of high-density and high-quality culture

Inactive Publication Date: 2015-02-18
GANSU DEFU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the organic carbon source added in the heterotrophic stage is selected from gluco...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, Heterotrophic cultivation of Chlorella in a mechanically stirred fermenter

[0026] Add heterotrophic culture solution with a pH of 6.5 to 7.5 in a mechanically stirred fermenter, the inoculum amount of Chlorella is 10% of the working volume, the culture temperature is 28 to 30°C, the pH is controlled within the range of 6.5 to 7.5, and the DO value (dissolved) is controlled. Oxygen content) in the range of 20% to 40%, until the chlorella cell density is the highest (45 ~ 55g / L) to end the culture, the chlorella is cultivated in the form of feed fed-batch culture, and the feed is heterotrophic culture The method of feeding is as follows: from the beginning of inoculation, feed 60mL at the 24h, 100mL at the 48h, 50mL at the 72h, and then 50mL every 24h until no longer grow. The heterotrophic culture process does not need light. Medium culture, and aseptic conditions are guaranteed, the heterotrophic culture medium is a liquid medium optimized on the basis o...

Embodiment 2

[0027] Embodiment 2, algae fluid dilution

[0028] The algal liquid obtained in the above-mentioned Example 1 was diluted with an autotrophic culture medium to a cell density of 15-25 g / L, and the pH was maintained at 6.5-7.5.

Embodiment 3

[0029] Embodiment 3, photoautotrophic cultivation of Chlorella

[0030] The dilution obtained in the above-mentioned Example 2 was transferred to photoautotrophic culture, the inoculum amount of Chlorella was 10% of the working volume, and the Chlorella was cultivated in the fed-batch culture form with feed, and the feed was autotrophic culture solution, and the supplement The feeding method is as follows: from the beginning of inoculation, feed 60mL at the 24th hour, 100mL at the 48th hour, 50mL at the 72nd hour, and then 50mL at intervals of 24 hours until the growth ceases. The culture temperature is 28-30°C, and the light intensity is 2000-5000lux , The light source of autotrophic culture process is natural light, adopts autotrophic culture medium, and its composition is urea 0.32g / L, potassium dihydrogen phosphate 0.156g / L, magnesium sulfate heptahydrate 0.146g / L, ferric chloride 0.001g / L.

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Abstract

The invention discloses a Chlorella autotrophic-heterotrophic mixed culture method, belonging to the technical field of biology. The method comprises the following steps: heterotrophic culture of Chlorella in mechanical-stirring fermentation tank, Chlorella solution dilution, and optical autotrophic culture of Chlorella. By using the method, the Chlorella can achieve high-density culture level in a short culture period, and the quality of Chlorella is greatly enhanced as compared with that of the Chlorella subjected to heterotrophic culture, thereby achieving the optical autotrophic culture level and implementing high-density high-quality culture of Chlorella.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for cultivating chlorella by autotrophic and heterotrophic mixing. Background technique [0002] Chlorella is a biological resource with important economic value and has great application and development potential. Chlorella is rich in protein, unsaturated fatty acids, carotenoids, lutein, astaxanthin and multivitamins, and has extremely high nutritional value and the function of improving immunity. [0003] People have a long history of the earliest research on spherules. Among them, Chlorella vulgaris and Chlorella pyrenoidosa are the algae species that have been studied more, and they are also the algae species commonly used in commercial production. [0004] At present, there are two commonly used culture methods for Chlorella: open outdoor large pond photoautotrophic culture and fermenter heterotrophic culture. Although the cost is low, the open outdoor la...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 冯涛赵晓霞
Owner GANSU DEFU BIOTECH
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