35 results about "Chlorella pyrenoidosa" patented technology

The invention provides a method for regulating and controlling growth and metabolism of caproic acid bacteria by using marine active peptides in a baijiu fermentation process. According to the method, the marine active peptides are added in an anaerobic fermentation stage of baijiu preparation to regulate and control the growth and metabolism of the caproic acid bacteria; wherein the marine active peptide is chlorella pyrenoidosa peptide, schizochytrium limacinum peptide, fish albumin peptide or euphausia superba peptide. According to the method, active peptides with specific sources are added into an anaerobic culture system, so that the growth of caproic acid bacteria can be promoted, energy metabolism can be enhanced, and the activity of key enzymes such as caproic acid dehydrogenase and caproic acid synthase can be activated, and therefore, the generation amount of flavor acids such as caproic acid and butyric acid can be increased, the fermentation quality can be improved, and the flavor level and esterification precursor substance content of Baijiu can be improved.

The invention discloses a decolorized chlorella protein, a preparation method thereof and application of the decolorized chlorella protein in preparation of foaming liquid, and relates to the technical field of biology. The preparation method comprises the following steps: extracting and decolorizing chlorella protein of chlorella pyrenoidosa, and drying to obtain the decolorized chlorella protein. According to the invention, an efficient chlorella protein extraction technology suitable for industrial production is established; the chlorella protein is treated by adopting an ethanol decolorizing method, the influence of two decolorizing methods and different decolorizing times on the decolorizing effect, the protein structure and the physicochemical property is systematically evaluated, the decolorized chlorella protein foaming liquid is prepared and represented, and the application effect of the decolorized chlorella protein foaming liquid in the foamed coffee is verified. Results show that the decolorized chlorella protein provided by the invention has excellent foaming characteristic, has the advantages of high protein purity and high sensory acceptance, and provides important technical support for efficient extraction of chlorella protein and widening of application range of chlorella.

A biochar made by a process including drying a Chlorella Pyrenoidosa feedstock for 2 to 4 hours to form a powder and mixing the powder with a carbonate salt at a weight ratio of 1:3 to 1:5. The process further includes pyrolyzing the powder and the carbonate salt in an inert atmosphere to a temperature of 500 degrees Celsius (° C.) to 800° C. at a heating rate of 5 degrees Celsius per minute (° C. / min) to 15° C. / min to form a product, sonicating the product with an acid to form a suspension, decanting the acid, washing and sonicating the product with water, and filtering and drying the product to form the biochar.

The present application relates to a kind of illumination culture methods for improving chlorella pyrenoidosa biomass, the illumination culture method includes the following steps: chlorella pyrenoidosa stock solution is inoculated in sterile liquid medium, and chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light.The present application develops a kind of culture method for chlorella pyrenoidosa, i.e.chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light, the culture method is simple to operate, easy to implement, the method can significantly improve the growth rate and biomass accumulation of chlorella pyrenoidosa, realize the high-quality large-scale production of chlorella pyrenoidosa;It can also reduce the production energy consumption per unit biomass, solves the technical problem that yield increase and consumption reduction are difficult to consider in microalgae culture.

The invention relates to a UDP sugar pyrophosphorylase CpUSP gene in the field of bioengineering and application of the UDP sugar pyrophosphorylase CpUSP gene in promoting harvesting and crushing of microalgae. The nucleotide sequence of the CpUSP gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the CpUSP gene is as shown in SEQ ID NO: 2. After the UDP-sugar pyrophosphorylase of the chlorella pyrenoidosa is over-expressed in the chlamydomonas, the cell morphology can be changed, the harvesting and breaking efficiency of the chlamydomonas is remarkably improved, and a new technical scheme is provided for reducing the harvesting and cell breaking cost of the microalgae.

The invention belongs to the technical field of algae development experiments, and particularly relates to a method for measuring energy metabolism of chlorella mitochondrial stress cells. According to the method, an Agilent cell energy meter XF24 is utilized, the setting of an Agilent default experimental program is optimized, and a mixing part in all reading programs is canceled; the drug concentrations of the three targeting drugs added into the mitochondrial electron transport chain are designed through a large number of research tests, and the number of measurement cycles (5-6 times) after the drugs are added is increased, so that the oxygen consumption rate of cells is measured to obtain key parameters reflecting the mitochondrial function, and the measurement result is more accurate. According to the method, a chlorella mitochondrial pressure curve graph is given, and a new research direction is provided for energy metabolism of algae cells.

The invention discloses a method for screening a chlorella pyrenoidosa mutant, which comprises the following steps: carrying out activation, culture and mutagenesis on chlorella pyrenoidosa to obtain a chlorella pyrenoidosa solution; wrapping the mutagenized algae liquid in micro-droplets, and collecting the micro-droplets in a conduit; culturing the wrapped micro-droplets under a given condition; and detecting a fluorescence signal of the cultured micro-droplet, and screening out chlorella pyrenoidosa with fast and slow growth rate based on the intensity of the fluorescence signal. The method has the beneficial effects of high flux, high precision and the like, and overcomes the defects of low flux and high false positive of the traditional method.

The application belongs to the technical field of water disinfection, and particularly relates to application of microalgae in killing Escherichia coli in wastewater, wherein the microalgae are one or more of Chlorella vulgaris Pediastrum ) and Chlorella pyrenoidosa Chlorella ). The application discloses for the first time that the pH and dissolved oxygen of the metabolism of the microalgae can synergistically inactivate the Escherichia coli, and play a disinfection role. In practical application, the removal rate of the Escherichia coli can reach 100%, the removal of pollutants such as TP and TN meets the discharge standard, the microalgae can independently treat the contaminated water, the removal of the pollutants does not need to be performed in advance through the conventional activated sludge method, and additional structures are not needed to kill the microorganisms in the water, so that the construction cost and operation cost are saved.

The invention relates to the technical field of food, in particular to microalgae emulsion and a preparation method thereof. The microalgae emulsion provided by the invention comprises chlorella pyrenoidosa subjected to green removal; and a liquid phase for dispersing the chlorella pyrenoidosa, wherein the liquid phase consists of water and corn oil. The chlorophyll content of the chlorella pyrenoidosa subjected to green removal is extremely low, meanwhile, the protein content of the chlorella pyrenoidosa subjected to green removal is extremely high, and the formed microalgae emulsion can be milky white, so that the adverse effect of conventional green chlorella on food sense organs can be reduced, and meanwhile, the green chlorella pyrenoidosa subjected to green removal is prepared based on the effect of a liquid phase composed of water and corn oil and the chlorella pyrenoidosa subjected to green removal. The microalgae emulsion disclosed by the invention also has good stability; therefore, the microalgae emulsion disclosed by the invention has very good stability and consumer acceptability, and has a very good application prospect in the field of foods.

The invention relates to the technical field of fermentation engineering, and discloses an organic wall-broken chlorella pyrenoidosa composite solid beverage and a preparation method thereof, the organic wall-broken chlorella pyrenoidosa composite solid beverage comprises the following raw materials by weight: 10-20 parts of wall-broken chlorella pyrenoidosa powder, 5-15 parts of fermentation auxiliary material dry powder, 3-8 parts of fructo-oligosaccharide, 5-10 parts of soybean protein isolate, 3-6 parts of coconut milk powder, 1-3 parts of lemon powder, and 0.001-0.002 part of selenomethionine. According to the technology, a complete technical chain is formed from raw material treatment to finished product packaging, efficient reservation of active ingredients, synergistic enhancement of functions, sensory quality optimization and application performance improvement are achieved, and the requirements for functional solid beverages in different scenes are met.

The invention provides a preparation method of a chlorella pyrenoidosa protein peptide-calcium chelate. The preparation method comprises the following steps: S1, dissolving chlorella pyrenoidosa protein in deionized water, adjusting the pH value to 2-13, stirring for 1-5 hours, centrifuging, and taking supernate to obtain a chlorella pyrenoidosa protein treatment solution; s2, adding calcium chloride into the chlorella pyrenoidosa protein treatment liquid, chelating in a water bath at 35-38 DEG C for 1.8-2.2 hours, centrifuging, adding absolute ethyl alcohol into supernate, standing, centrifuging again, collecting precipitate, and freeze-drying to obtain the chlorella pyrenoidosa protein treatment liquid. The preparation method is simple in preparation process, free of toxic and side reagents and low in energy consumption, the calcium absorption promoting effect of the prepared product is verified through animal experiments, and a solid theoretical support is provided for development and application of the chlorella pyrenoidosa protein peptide-calcium chelate as a novel bone health promoter.

The invention provides a yellow chlorella pyrenoidosa mutant strain APMT04 and an application of the yellow chlorella pyrenoidosa mutant strain. The chlorella pyrenoidosa mutant strain APMT04 is obtained through a chemical mutagenesis method, compared with an original strain, the chlorella pyrenoidosa mutant strain APMT04 has the advantages that the growth speed of chlorella cells under the heterotrophic condition is higher, and meanwhile, the chlorophyll content of the chlorella pyrenoidosa mutant strain APMT04 is reduced by 90% or above, but the chlorella pyrenoidosa mutant strain APMT04 contains high-content xanthophyll, so that the And the extract also contains high-content protein and grease, can be used as byproducts of xanthophyll extraction, and can be used in the fields of food, feed and the like. When the chlorella pyrenoidosa mutant strain APMT04 is used for producing xanthophyll, the later-stage extraction and preparation process of xanthophyll can be simplified, the sensory acceptance of algae powder is improved, protein and grease rich in the algae powder can serve as by-products of xanthophyll extraction, and high-quality germplasm resources are provided for industrial production and application of microalgae xanthophyll.

The application belongs to the technical field of soil remediation, and discloses a microalgae biological soil remediation agent and application thereof.A sustainable benign underground micro-ecosystem is constructed, and the prepared microalgae biological soil remediation agent takes a porous active carrier with positive charges as a matrix.The porous active carrier is obtained by compounding silicon dioxide on montmorillonite, and the porous active carrier is modified to obtain an amine group of a negative charge NH2 ‑ between layers, and finally loaded with chitosan cations to become a porous active carrier with positive charges, which is loaded with a large amount of Chlorella pyrenoidosa with reduced environmental sensitivity, so that it can survive and reproduce in harsh soil environments, can significantly improve soil fertility, improve crop yield and quality, and also has the effect of reducing soil heavy metal pollution; can also increase the diversity of soil microbial communities, improve soil aggregate structure, and improve the soil physical and chemical environment.

The invention discloses a method for producing high-organic-calcium microalgae single-cell protein by recycling white spirit vinasse, which comprises the following steps: by taking the white spirit vinasse as a nutrient medium and chlorella pyrenoidosa as a single-cell protein source, firstly, pretreating the white spirit vinasse and carrying out microbial acid-producing fermentation, and dissolving the organic acid generated by fermentation into low-cost inorganic calcium (calcium carbonate) in a system to obtain the high-organic-calcium microalgae single-cell protein. When the pH of the system is adjusted and the acid inhibition in the fermentation process is relieved, an available calcium source and an organic acid carbon source are provided for the chlorella pyrenoidosa; then carrying out directional domestication breeding on the chlorella pyrenoidosa to improve the tolerance, the growth efficiency and the calcium enrichment rate of the chlorella pyrenoidosa; and finally, culturing the domesticated chlorella pyrenoidosa by using a vinasse culture medium subjected to fermentation conversion to produce microalgae single-cell protein rich in organic calcium. According to the invention, the resource reutilization of food wastes is realized, the microalgae culture cost is reduced, the organic calcium content in the obtained single-cell protein product is increased (greater than or equal to 0.48%), and the single-cell protein product can be used for preparing functional foods.

The invention discloses a chlorella heptapeptide TGPLGSR with multi-target lipid-lowering activity as well as a preparation method and application of the chlorella heptapeptide TGPLGSR. The amino acid sequence of the heptapeptide is Thr-Gly-Pro-Leu-Gly-Ser-Arg, and the amino acid sequence of the heptapeptide is as shown in the specification. The preparation method comprises the following steps: performing ultraviolet and electric shock compound mutagenesis on wild chlorella pyrenoidosa to obtain a mutagenic algal strain with high protein content; carrying out fermentation and alcoholase synergistic extraction and purification to obtain algae protein; after protein is subjected to directional enzymolysis by adopting alkaline protease, active peptide components are enriched through ultrafiltration and gel chromatography in sequence; finally, the heptapeptide is obtained through liquid chromatography-mass spectrometry identification, computer-aided prediction and molecular docking technology screening. The heptapeptide has strong inhibitory activity on pancreatic lipase and can reduce accumulation of triglyceride and cholesterol in 3T3-L1 cells in a dose-dependent manner, the mechanism of the heptapeptide relates to multi-target pathways such as MMP-9 inhibition, AMPK phosphorylation activation and PNLIP down-regulation, and the heptapeptide can be used for developing lipid-lowering functional foods or health-care products.

The invention discloses a composition containing agilawood extract and a preparation method and application thereof, and belongs to the technical field of biology, the composition comprises 5-20 parts of agilawood extract, 20-60 parts of functional peptide and 5-25 parts of seaweed functional component; wherein the seaweed functional component is used for forming an embedding, gel or slow-release structure; the functional peptide is preferably chlorella pyrenoidosa peptide and is prepared by combining freeze-thaw ultrasonic-assisted enzymolysis with cation exchange chromatography; the agilawood extract is preferably an agilawood chromone enriched extract; during preparation, the seaweed functional component is dissolved in water, the agilawood extract is added for dispersion or emulsification, then the functional peptide is added for uniform mixing, and freeze-drying, spray-drying or forming treatment is performed to obtain the seaweed functional peptide. The composition can effectively improve the stability and bioavailability of active ingredients, and has a good application prospect in preparation of products for auxiliary protection of acute, alcoholic, drug-induced or fatty liver injuries.

The application discloses application of an iron-manganese-based composite microalgae biochar material as a phosphorus removal adsorbent in treatment of phosphorus-containing wastewater, and a preparation method of the iron-manganese-based composite microalgae biochar material is as follows: Chlorella pyrenoidosa powder is immersed in a mixed solution of ferric chloride, manganese sulfate and EDTA, and then dried, ground and pyrolyzed to obtain the iron-manganese-based composite microalgae biochar material. The biochar has a large specific surface area, is rich in oxygen-containing functional groups and has many adsorption sites, the addition of ferric chloride makes the biochar magnetic and easy to recover, the addition of manganese sulfate increases the specific surface area and oxygen-containing functional groups of the biochar, the biochar structure is a flaky accumulated aggregate, and the biochar has strong adsorption capacity and can be applied to treatment of phosphorus-containing polluted water bodies. The addition of EDTA makes the iron and manganese better loaded on the microalgae, and can make the biochar produce specific chemical adsorption with phosphate, and further improves the adsorption performance of the material, and provides a new way and thought for removal of phosphate in an aquatic environment and recycling of harmful algae.

This invention relates to a Chlorella mutant strain that is easily separated by sedimentation and cell wall disruption, and its uses, in the fields of biology and bioengineering. The Chlorella is... Chlorella pyrenoidosa GA, deposited on March 23, 2026 at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M2026500. This Chlorella mutant strain showed no difference in growth compared to the wild type under all culture conditions, but exhibited a significantly increased protein accumulation. Furthermore, the mutant cells settled more easily, with significantly higher natural sedimentation and centrifugation efficiencies under the same treatment conditions compared to the wild-type strain. Cell disruption was also significantly easier, facilitating digestion and absorption by predators. The mutant strain described in this invention has broad application prospects in the production of Chlorella-related products and aquaculture.

The invention aims to provide a chlorella pyrenoidosa peptide with an effect of preventing alcoholic liver injury, which is prepared by the following steps: crushing chlorella pyrenoidosa, mixing with water, freezing and thawing, and performing ultrasonic treatment to prepare homogenate; alkaline protease is added for enzymolysis, and then flavourzyme is added for enzymolysis; and after the enzymolysis product is adsorbed by activated carbon, the supernate is adsorbed by using an SP Sephadex C25 cation exchange medium. The amino acid sequence of the chlorella pyrenoidosa peptide is PSEQ ID NO: 1-5. The chlorella pyrenoidosa peptide provided by the invention is obtained by enzymolysis, adsorption and cation exchange chromatography technologies, and is verified by a HepG2 cell model to obviously relieve alcoholic liver injury. The raw materials are natural and safe, the process is accurate and efficient, and industrialization potential is achieved. Meanwhile, the peptide is suitable for development of functional food, health care products and medicines, and a low-toxicity and sustainable liver protection solution is provided.

The invention discloses a water quality purification phycomycete composition, a water quality purification system and application, and belongs to the technical field of water treatment and ecological restoration. The phycomycete composition for water quality purification comprises mixed microalgae and a composite flora, the mixed microalgae comprise chlorella sorojin, chlorella vulgaris, chlorella pyrenoidosa, chlamydomonas reinhardtii and chlamydomonas plana; the composite flora comprises bread yeast, bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis and pseudomonas putida. According to the invention, dominant algae species in an in-situ water body and external composite flora are combined as a water quality purification phycomycete composition, so that not only is the removal efficiency of nutritive salt improved, but also the increase of microalgae biomass is promoted by improving the phycomycete microbial environment and adjusting the ecological structure. The phycomycete composition disclosed by the invention can be directly used for in-situ treatment of rivers in cold regions, is directly arranged in rivers, bank zones or constructed wetlands, and overcomes the defect that a traditional process is difficult to efficiently and stably operate in treatment of rivers in cold regions.

The application provides application of Chlorella algae strains in Macrobrachium rosenbergii breeding and virus inhibition, which is that the Chlorella algae strains are expanded in an optimized biogas liquid culture system, and the algal body or algal liquid is collected and put into the water body of the Macrobrachium rosenbergii breeding; the Chlorella algae strains are selected from Chlorella pyrenoidosa Chlorella pyrenoidosa , or Chlorella sorokiniana Chlorella sorokiniana . The application finds that if disease prevention and control is the core, Chlorella pyrenoidosa is preferred; if promoting growth and improving yield is the core, Chlorella sorokiniana is preferred; and Chlorella pyrenoidosa and Chlorella sorokiniana have significant and broad-spectrum direct antiviral activity.

The invention belongs to the technical field of veterinary biological products, and discloses chlorella pyrenoidosa CV-5, a probiotic microcapsule and application of the probiotic microcapsule in preparation of products for improving intestinal health of calves. The preservation number of the chlorella pyrenoidosa CV-5 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.15206, and the Latin name of the chlorella pyrenoidosa CV-5 provided by the invention is Chlorella pyrenoidosa. Compared with other algae, the chlorella pyrenoidosa CV-5 provided by the invention has remarkable advantages in the aspect of improving the intestinal health of calves. On the basis, chlorella pyrenoidosa CV-5 powder and pectin are matched with glucose oxidase to serve as wall materials, dominant probiotic strains, sodium butyrate, trypsin and specific prebiotics serve as core materials, and the feed capable of remarkably improving the survival rate of the probiotics in the calf wrinkled stomach environment, improving the survival rate of the probiotics in the calf wrinkled stomach environment and improving the survival rate of the probiotics in the calf wrinkled stomach environment is further developed. Meanwhile, the abundance of pathogenic bacteria in intestinal tracts of the diarrhea calves is reduced. The feed additive is suitable for the livestock breeding industry, and especially has a good application prospect in the field of improving the intestinal health of the calves.

The application discloses a Lactiplantibacillus plantarum and application of the Lactiplantibacillus plantarum in fermentation beverage of Chlorella pyrenoidosa. In the application, a Lactiplantibacillus plantarum is screened and separated from an outdoor Chlorella pyrenoidosa cultivation pond, and is named as Lactiplantibacillus plantarum HN3. The Lactiplantibacillus plantarum HN3 has been preserved in the Guangdong Microbial Culture Collection Center, No. 59 Building, 5th Floor, Guangzhou City, on July 26, 2024, and has a preservation number of GDMCC No: 64816. The Lactiplantibacillus plantarum HN3 has good fermentation performance, acid tolerance and bile salt tolerance, can degrade bad odor substances of Chlorella pyrenoidosa liquid, produce new pleasant odor substances, and can be used as a production strain of Chlorella pyrenoidosa fermentation beverage.

The invention belongs to the technical field of chlorella deep processing, and particularly relates to a chlorella pyrenoidosa precise active peptide capable of promoting GLP1 secretion, reducing blood sugar and lipid and enhancing immunity, and a preparation method and application thereof. The preparation method comprises the following steps: firstly, mixing chlorella pyrenoidosa powder with water, and pretreating at high temperature; after pretreatment, cellulase, pectinase and alkaline protease 1 are sequentially added for hydrolysis, and then the chlorella pyrenoidosa peptide is obtained through enzyme deactivation, centrifugal filtration, drying and powder spraying. The chlorella pyrenoidosa peptide prepared by the method disclosed by the invention has the effects of resisting oxidation, inhibiting DPP-IV activity, promoting GLP-1 secretion, enhancing immunity and the like. The preparation method of the chlorella precise active peptide provided by the invention has the advantages of low equipment requirement, simplicity and convenience in operation, easiness in industrial amplification and the like, and a feasible technical scheme is provided for large-scale production of functional active peptides.

The invention discloses a preparation method and application of a chlorella pyrenoidosa polypeptide-polyphenol composite antioxidant. The preparation method is characterized by comprising the following steps: a) extracting protein from chlorella pyrenoidosa by using an alkali-solution and acid-isolation method; b) performing enzymolysis on the protein obtained in the step a) by using alkaline protease to obtain polypeptide; and c) adding polyphenol into the polypeptide, and mixing to obtain the composite antioxidant with higher antioxidant activity. Wherein the final concentration of the chlorella pyrenoidosa polypeptide is 1mg / mL, and the final concentration of the gallic acid is 1, 2, 3, 4 and 5 mu g / mL; the final concentration of the chlorogenic acid is 5, 10, 15, 20, 25, 30 and 35 mu g / mL; the final concentration of EGCG is 2, 4, 6, 8, 10 [mu] g / mL. The invention is beneficial to the development and application of the chlorella pyrenoidosa polypeptide-polyphenol composite antioxidant in the fields of food, medicines and the like.

The application provides a three-dimensional multi-stage hole phosphonated chitosan / microalgae-based aerogel and a preparation method and application thereof, and belongs to the technical field of aerogel preparation. The three-dimensional multi-stage hole phosphonated chitosan / microalgae-based aerogel (aCSP / CP) is prepared by taking chitosan (CS), phosphonated chitosan (CSP) and chlorella pyrenoidosa powder (CP) as raw materials. The selectivity of CP to U(VI) is low, but the total ion adsorption capacity is higher, and more coexisting ions can be adsorbed. The selectivity of CSP to U(VI) is greatly improved. The high affinity and high selectivity between the phosphorus functional groups in CSP and U(VI) can improve the selective adsorption of U(VI). In the application, CP and CSP synergistically act together to realize the high selective adsorption of U(VI).

The application provides a preparation method of Chlorella pyrenoidosa protein peptide-calcium chelate, and the preparation method comprises the following steps: S1, Chlorella pyrenoidosa protein is dissolved in deionized water, the pH is adjusted to 2-13, stirring is carried out for 1-5 hours, centrifugation is carried out, the supernatant is taken, and a Chlorella pyrenoidosa protein treatment solution is obtained; S2, calcium chloride is added to the Chlorella pyrenoidosa protein treatment solution, water bath chelation is carried out at 35-38 DEG C for 1.8-2.2 hours, centrifugation is carried out, anhydrous ethanol is added to the supernatant, standing is carried out, centrifugation is carried out again, the precipitate is collected, and freeze-drying is carried out, and the Chlorella pyrenoidosa protein peptide-calcium chelate is obtained. The preparation process is simple, no toxic side reagent is used, energy consumption is low, the prepared product is verified to have a calcium absorption promoting effect through animal experiments, and solid theoretical support is provided for the development and application of the Chlorella pyrenoidosa protein peptide-calcium chelate as a new bone health promoter.

The invention relates to the technical field of microbial culture technology and food processing science, in particular to an efficient and rapid culture method for chlorella pyrenoidosa rice, which comprises the following steps: step 1, gelatinizing a rice matrix part; step 2, carrying out charge reversal activation on the algae species: carrying out low-shear propagation in a culture medium containing rice starch hydrolysate to an inflection point in a logarithmic phase; adding cationic polysaccharide until the Zeta potential on the surface of the algae cell reaches a charge zero point; step 3, performing negative pressure permeation inoculation; 4, light-gas coupling culture: red light and blue light are alternately irradiated, bottom airflow associated with the thickness of the rice bed is started in the red light period, and humidity control and humidification are started in the blue light period; the culture temperature is dynamically increased based on chlorophyll fluorescence intensity change; and step 5, enzyme-magnetic synergistic harvesting. According to the method, the field planting efficiency of the chlorella pyrenoidosa in the rice matrix is effectively improved, and the growth cycle of algae is accelerated.

The invention relates to the technical field of microalgae biology, in particular to a high-biomass and high-photosynthetic-efficiency chlorella pyrenoidosa mutant strain J-1 which is obtained by mutagenizing wild type chlorella pyrenoidosa through an atmospheric pressure room temperature plasma (ARTP) mutagenesis technology and combining an efficient screening strategy. When the strain is cultured for 4 days under a standard autotrophic condition, the biomass reaches 1.23 mg / mL, the OD750 reaches 6.27, and when the strain is cultured for 5 days under a high light stress condition, the OD750 reaches 16.70; according to the invention, the growth performance is obviously enhanced: the biomass of J-1 is higher than that of WT under autotrophic, mixotrophic or heterotrophic conditions, and the biomass of a J-1 mutant strain reaches 1.23 mg / mL and is increased by 53.8% compared with that of a wild type (0.80 mg / mL) after the J-1 mutant strain is cultured for 4 days under a standard autotrophic condition (50 [mu] mol * m <-2 > * s <-1 >); the OD750 reaches 6.27 and is higher than 5.75 of a wild type, the OD750 reaches 2.18 mg / mL (WT is 1.75 mg / mL) on the fourth day of mixotrophic culture, the OD750 reaches 1.39 mg / mL (WT is 1.05 mg / mL) on the fourth day of heterotrophic culture, and it is indicated that biomass can be efficiently accumulated in different nutritional modes.

The invention belongs to the technical field of biology, and particularly relates to an ecological edible mushroom photosynthetic oxygen evolution composition as well as a preparation method and application thereof. The ecological edible mushroom photosynthetic oxygen evolution composition is prepared from the following raw materials: a heterozygous algae component, an amino acid component, a sugar component and a biological silicon component, wherein the heterothecium component is prepared from the following raw materials: small scochium, needle cystis, yellow algae, brachypodium, silothecium, floating algae, fast-running algae, box-type algae, stick-type algae and brown-branch algae; the amino acid component comprises the following raw materials: phenylalanine, proline, glutamic acid, tryptophan and arginine; the sugar component comprises the following raw materials: monosaccharide, disaccharide, polysaccharide and zymosan; the biological silicon component comprises the following raw materials: plant silicon and animal silicon. The ecological edible mushroom photosynthetic oxygen evolution composition contains rich synthetic oxygen evolution cells (more than 6 billion / ml), can accelerate conversion of carbon source substances into carbohydrates of monosaccharide which can be directly absorbed and utilized by entities, and is environment-friendly, low in application cost and good in economic benefit.