66 results about "Chlorella pyrenoidosa" patented technology

The invention discloses a preparation method of microalgae nano-selenium and application of biotransformed nano-selenium in prevention and treatment of plant late blight. The novel preparation method of the microalgae nano-selenium comprises the following steps: firstly, extracting an intracellular extract of chlorella pyrenoidosa, and then adding sodium selenite into the intracellular extract to carry out nano-selenium conversion; compared with an existing reported method that after sodium selenite is added into a microalgae living body culture solution to be converted into nano-selenium, algae is taken to break cells, and the nano-selenium is collected, the method has the advantages that the microalgae living body does not need to be used when the nano-selenium is biologically converted, and the subsequent complicated nano-selenium separation and purification steps can be avoided; the nano-selenium obtained by converting the intracellular extract can be directly applied. Experiments prove that the effect of the nano-selenium obtained through biotransformation on inhibiting phytophthora infestans of potato late blight pathogenic bacteria is obviously better than that of chemically synthesized nano-selenium, and the nano-selenium obtained through biotransformation has an obvious effect on preventing and treating potato late blight and has an obvious effect on promoting plant growth.

The invention discloses an EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and an application thereof. The preparation method comprises the following steps: mutagenizing a chlorella pyrenoidosa mutant strain CX41 by adopting an ethyl methanesulfonate (EMS) and normal-pressure and room-temperature plasma (ARTP) composite technology, screening and verifying to obtain a golden yellow chlorella pyrenoidosa mutant strain, naming the golden yellow chlorella pyrenoidosa mutant strain as chlorella pyrenoidosa Y11, and screening and verifying the golden yellow chlorella pyrenoidosa Y11 to obtain the chlorella pyrenoidosa Y11. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou on October 23, 2024, and the preservation number is GDMCC No: 65324. The chlorella pyrenoidosa mutant strain Y11 has the characteristics of high yield of protein and rich carotenoids, and can be used for fermentation production of microalgae protein and carotenoids.

The invention provides a chlorella pyrenoidosa calcium chelating peptide and a chlorella pyrenoidosa peptide calcium chelate prepared by using the chlorella pyrenoidosa calcium chelating peptide. The prepared chlorella pyrenoidosa peptide calcium chelate can enhance the maximum load and structural strength of the tibia of a mouse with osteoporosis. The chlorella pyrenoidosa calcium chelating peptide provided by the invention is combined with calcium ions to form a peptide-calcium chelate, and animal experiments prove that the peptide-calcium chelate can significantly improve the maximum load and structural strength of the tibia of a mouse with osteoporosis, and has a good effect of improving the bone biomechanical properties of the mouse with osteoporosis.

The invention discloses a chlorella pyrenoidosa white mutant strain FZU65-W1 and application of the chlorella pyrenoidosa white mutant The classification name of the chlorella pyrenoidosa white mutant strain FZU65-W1 is chlorella pyrenoidosa, the chlorella pyrenoidosa white mutant strain is preserved in the China General Microbiological Culture Collection Center on September 11, 2024, the preservation number is CGMCC N0.46151, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with an original strain FZU65, the chlorella pyrenoidosa white mutant strain FZU65-W1 has the advantages that the growth speed of algae cells under the heterotrophic condition is higher, and meanwhile, the cells are white due to the fact that chlorophyll and carotenoid are not synthesized in the cells. The chlorella pyrenoidosa white mutant strain FZU65-W1 can be used for producing high-quality protein through heterotrophic fermentation, so that not only can a later-stage protein extraction and preparation process be simplified, but also the sensory acceptance of algae powder can be improved, and a high-quality germplasm resource is provided for industrial production and application of microalgae protein.

The invention relates to the technical field of microalgae heterotrophic culture and biomass proliferation, in particular to a chlorella pyrenoidosa heterotrophic high-density proliferation method. The specific technical scheme is as follows: the heterotrophic high-density proliferation method of chlorella pyrenoidosa comprises the following steps: pre-culture and heterotrophic propagation of chlorella pyrenoidosa: inoculating a chlorella pyrenoidosa solution into a BG11 culture medium, and culturing for 3-4 days in a constant-temperature shaking table with the temperature of 25 + / -1 DEG C, the rotating speed of 200rpm and the light-dark ratio of 0h: 24h to obtain a primary culture chlorella solution; inoculating the primary culture algae liquid into a pre-sterilized and optimized heterotrophic culture medium, and carrying out expanding culture under the same culture condition for 3-4 days to obtain a secondary expanding culture algae liquid; (2) a heterotrophic high-density culture stage: regularly supplementing a nutrient culture medium containing high-concentration glucose, nitrate and phosphate by adopting an index fed-batch feeding strategy; other conditions are kept consistent during culture; and (3) an in-situ protein accumulation induction stage: when the chlorella pyrenoidosa reaches an exponential growth end stage, adding paclobutrazol into the system, and keeping other conditions consistent while keeping exponential fed-batch culture. According to the method disclosed by the invention, the biomass of the chlorella pyrenoidosa is increased by about 128.6% compared with that of the traditional batch culture, the protein content can reach 49.14%, meanwhile, the configuration of essential amino acids is improved, and the requirements of high-end biomass resource application are met. The method has the advantages of simplicity and convenience in operation and high production efficiency, and is suitable for the fields of high-quality protein extraction and related biomass production.

According to the chlorella pyrenoidosa high-F-value peptide and the preparation method thereof, parameters in the enzymolysis and adsorption processes are strictly controlled, and the enzymolysis of the incision enzyme and the excision enzyme not only can efficiently hydrolyze chlorella protein and generate more peptide fragments with biological activity, but also can be used for preparing the chlorella pyrenoidosa high-F-value peptide. And the characteristics of high industrial production cost and poor stability caused by wide enzyme cutting sites are solved. The prepared chlorella pyrenoidosa high-F-value peptide reaches 36.90 + / -8.18, the F value of the chlorella pyrenoidosa high-F-value peptide is increased by 12.72 times compared with the chlorella pyrenoidosa raw material (2.90 + / -0.07), and in addition, the molecular weight of the high-F-value oligopeptide is 1t; peptides with the molecular weight of 1000 Da account for 95-99.40%, the characteristics of oligopeptides are met, the molecular weight is small, and digestion and absorption are easy. The prepared high-F-value oligopeptide can accelerate metabolism of ethyl alcohol in the liver and protect the liver from being damaged by the ethyl alcohol, and has the effects of dispelling the effects of alcohol and protecting the liver.

The invention aims to provide a chlorella pyrenoidosa peptide with an effect of preventing alcoholic liver injury, which is prepared by the following steps: crushing chlorella pyrenoidosa, mixing with water, freezing and thawing, and performing ultrasonic treatment to prepare homogenate; alkaline protease is added for enzymolysis, and then flavourzyme is added for enzymolysis; and after the enzymolysis product is adsorbed by activated carbon, the supernate is adsorbed by using an SP Sephadex C25 cation exchange medium. The amino acid sequence of the chlorella pyrenoidosa peptide is PSEQ ID NO: 1-5. The chlorella pyrenoidosa peptide provided by the invention is obtained by enzymolysis, adsorption and cation exchange chromatography technologies, and is verified by a HepG2 cell model to obviously relieve alcoholic liver injury. The raw materials are natural and safe, the process is accurate and efficient, and industrialization potential is achieved. Meanwhile, the peptide is suitable for development of functional food, health care products and medicines, and a low-toxicity and sustainable liver protection solution is provided.

The invention discloses double-layer double-color solidification type fermented milk and a preparation method thereof, and belongs to the technical field of dairy product processing. According to the preparation method of the double-layer double-color solidification type fermented milk, chlorella pyrenoidosa is added on the basis of traditional solidification type fermentation, and double-layer solidification type mixed fermentation is adopted. According to the double-layer double-color solidification type fermented milk disclosed by the invention, a coagulant and other additives do not need to be added, and a fermented product is good in texture, relatively good in solidification form and relatively small in whey precipitation by controlling a fermentation strain and the fermentation time of the fermentation strain.

The invention provides a method for regulating and controlling growth and metabolism of caproic acid bacteria by using marine active peptides in a baijiu fermentation process. According to the method, the marine active peptides are added in an anaerobic fermentation stage of baijiu preparation to regulate and control the growth and metabolism of the caproic acid bacteria; wherein the marine active peptide is chlorella pyrenoidosa peptide, schizochytrium limacinum peptide, fish albumin peptide or euphausia superba peptide. According to the method, active peptides with specific sources are added into an anaerobic culture system, so that the growth of caproic acid bacteria can be promoted, energy metabolism can be enhanced, and the activity of key enzymes such as caproic acid dehydrogenase and caproic acid synthase can be activated, and therefore, the generation amount of flavor acids such as caproic acid and butyric acid can be increased, the fermentation quality can be improved, and the flavor level and esterification precursor substance content of Baijiu can be improved.

The invention discloses a decolorized chlorella protein, a preparation method thereof and application of the decolorized chlorella protein in preparation of foaming liquid, and relates to the technical field of biology. The preparation method comprises the following steps: extracting and decolorizing chlorella protein of chlorella pyrenoidosa, and drying to obtain the decolorized chlorella protein. According to the invention, an efficient chlorella protein extraction technology suitable for industrial production is established; the chlorella protein is treated by adopting an ethanol decolorizing method, the influence of two decolorizing methods and different decolorizing times on the decolorizing effect, the protein structure and the physicochemical property is systematically evaluated, the decolorized chlorella protein foaming liquid is prepared and represented, and the application effect of the decolorized chlorella protein foaming liquid in the foamed coffee is verified. Results show that the decolorized chlorella protein provided by the invention has excellent foaming characteristic, has the advantages of high protein purity and high sensory acceptance, and provides important technical support for efficient extraction of chlorella protein and widening of application range of chlorella.

The invention provides a method for producing an extracellular polymeric substance by using an algal-bacterial symbiotic system, and belongs to the technical field of industrial biology. The method for producing the extracellular polymeric substance by utilizing the algal-bacterial symbiotic system comprises the following steps: inoculating chlorella pyrenoidosa and lactobacillus paracasei into a BG11 liquid culture medium, culturing under the conditions that the temperature is 27-29 DEG C, the rotating speed is 160-200rpm, the illumination intensity is 3800-4200LX and the illumination ratio is 24h: 0h, then respectively collecting supernate 1 and thalli, resuspending the thalli, collecting supernate 2, and carrying out freeze-drying to obtain the extracellular polymeric substance. Enabling the supernatant 1 and the supernatant 2 to pass through a filter membrane respectively, and collecting filtrate to obtain the traditional Chinese medicine composition. When the concentration of glucose in the G11 liquid culture medium is 3.8-4.2 g / L, the pH value is 4.7-5.2, and the ratio of bacteria to algae is (0.8-1.2): 1, the growth condition of the bacteria-algae symbiotic system is optimal, and when the concentration of sodium selenite is 2-4 mg / L, the yield of the extracellular polymeric substance is highest, and efficient preparation of the extracellular polymeric substance is realized.

The application provides a Chlorella pyrenoidosa polypeptide and application thereof, the Chlorella pyrenoidosa polypeptide has high immunocompetence, and can be used for preparing products with immune function and rehabilitation promotion.The application takes Chlorella pyrenoidosa as raw material, adopts complex enzymolysis technology, is separated and purified through Sephadex G-25 gel column chromatography and C18 reverse phase high performance liquid chromatography, is identified in sequence through HPLC-MS / MS, is combined with Peptide Ranker immunocompetence prediction and AutoDock molecular docking, takes active score as screening index, and determines that the core high immunocompetence peptide segment is VEFYGPDR and RAPGAPGAVP.SPF level ICR mouse spleen lymphocytes are separated, and the influence of high immunocompetence peptide segment on spleen lymphocyte, T lymphocyte and B lymphocyte proliferation activity under the stimulation of ConA / LPS is detected by MTT method.The results show that the two peptide segments can significantly improve spleen lymphocyte proliferation 147.86%-243.75%, T lymphocyte proliferation 116.73%-121.88% and B lymphocyte proliferation 159.88%-161.01%, which proves that the two peptide segments have immune regulation function.

A biochar made by a process including drying a Chlorella Pyrenoidosa feedstock for 2 to 4 hours to form a powder and mixing the powder with a carbonate salt at a weight ratio of 1:3 to 1:5. The process further includes pyrolyzing the powder and the carbonate salt in an inert atmosphere to a temperature of 500 degrees Celsius (° C.) to 800° C. at a heating rate of 5 degrees Celsius per minute (° C. / min) to 15° C. / min to form a product, sonicating the product with an acid to form a suspension, decanting the acid, washing and sonicating the product with water, and filtering and drying the product to form the biochar.

The present application relates to a kind of illumination culture methods for improving chlorella pyrenoidosa biomass, the illumination culture method includes the following steps: chlorella pyrenoidosa stock solution is inoculated in sterile liquid medium, and chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light.The present application develops a kind of culture method for chlorella pyrenoidosa, i.e.chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light, the culture method is simple to operate, easy to implement, the method can significantly improve the growth rate and biomass accumulation of chlorella pyrenoidosa, realize the high-quality large-scale production of chlorella pyrenoidosa;It can also reduce the production energy consumption per unit biomass, solves the technical problem that yield increase and consumption reduction are difficult to consider in microalgae culture.

The invention provides a skin care composition based on a mixed chlorella extract as well as a preparation method and application of the skin care composition, and belongs to the technical field of cosmetics. The chlorella essence is prepared from the following raw material components in percentage by weight: 7%-15% of a mixed chlorella extract, 4%-10% of a synergist, 12%-30% of a humectant, 2.0%-8.0% of an emulsifier, 0.3%-0.6% of a thickening agent, 0.5%-1.2% of a preservative, 0.01%-0.4% of perfume and the balance of deionized water, the mixed chlorella extract is obtained by extracting chlorella pyrenoidosa, chlorella vulgaris and chlorella ellipsosa as raw materials. The chlorella pyrenoidosa, the chlorella vulgaris and the chlorella ellipsosa are used as raw materials to prepare the mixed chlorella extract, multiple effects and synergistic effects of the mixed chlorella extract are fully exerted, and the skin moisturizing and repairing effects are remarkably improved.

The application discloses a microalgae preparation group for greenhouse gas emission reduction in a rice field and a greenhouse gas emission reduction method in a rice field, and relates to the technical field of agricultural greenhouse gas emission reduction. In view of the technical problems that photosynthesis oxygen release of a single algae species is limited by a temperature window, it is difficult to cover the whole process during a methane double-peak emission period in the rice field, and nitrous oxide cannot be reduced simultaneously, the application provides a microalgae preparation group, which comprises a first preparation and a second preparation, both of which comprise chlorella vulgaris, atropteris varians and chlorella pyrenoidosa; the concentration ratio of chlorella pyrenoidosa to chlorella vulgaris in the first preparation is higher, the concentration ratio of chlorella vulgaris to chlorella pyrenoidosa in the second preparation is higher, and the total concentration is 0.5*10 7 to 5*10 7 million cells / mL; and the method is to apply the first preparation at 2.5-3.5 L / mu after the rice field is rewatered after returning green, and to apply the second preparation at 1.5-2.5 L / mu after the tillering ends. The application can significantly reduce the cumulative emission amount of methane and nitrous oxide, and has a yield-increasing effect.

The invention relates to the technical field of chlorella protein powder, and provides anti-aging and anti-fatigue chlorella pyrenoidosa protein polypeptide powder and a preparation method thereof.The anti-aging and anti-fatigue chlorella pyrenoidosa protein polypeptide powder is prepared from, by weight, 80%-90% of chlorella protein, 5%-15% of collagen and 1%-5% of oxalic acid degrading enzyme. The chlorella protein contains various minerals such as calcium, iron, zinc and selenium, and the oxalic acid degrading enzyme can degrade oxalic acid in the chlorella protein to reduce the content of oxalic acid, so that the situation that the oxalic acid is combined with the minerals such as calcium and iron to affect absorption of the minerals such as calcium and iron by a human body can be avoided, and the health-care effect is greatly improved.

A method for improving hydrogen production efficiency by using Chlorella motors, belonging to the field of bioenergy technology. The present invention is to solve the problems that after the formation of Chlorella aggregates, it is difficult to redisperse them, resulting in a decrease in the acquisition rate of external electrons by internal Chlorella, a decrease in light energy utilization rate, and a decrease in its own survival rate, etc. The method is as follows: Using Chlorella pyrenoidosa as the living motor matrix, PDA-modified Ti3C2 two-dimensional material as the main shell material, and titanium dioxide (TiO2) nanomaterial as the motor driving material, a light-driven Chlorella motor with a yin-yang structure is constructed. By using its controllable "dispersion-aggregation" cyclic behavior, the effect of "accumulating electrons for hydrogen production when dispersed - consuming electrons for efficient hydrogen production when aggregated" of Chlorella is realized, thereby improving the survival rate, nutrient utilization rate and hydrogen production efficiency. The construction of this system is simple and the process is mild. The Ti3C2 shell has a protective effect on the biological activity and function of Chlorella, and the TiO2 shell has a photocatalytic function and a protective function.

The invention provides a process for producing an indoleacetic acid organic fertilizer by utilizing microalgae, which comprises the following steps: A, putting pig manure, chlorella pyrenoidosa solution and hermetia illucens larvae into a treatment pond, and uniformly stirring until the concentration of the chlorella pyrenoidosa solution is not lower than 1 * 10 < 8 > / L; the addition amount of the chlorella pyrenoidosa solution is 10-30 liters per ton of pig manure, and the addition amount of the hermetia illucens larvae is 600,000-120,000 per ton of pig manure; b, pig manure and chlorella pyrenoidosa liquid are added into the treatment pond according to a plurality of preset time; c, separating hermetia illucens larvae and insect sand within the preset time after the pig manure and the chlorella pyrenoidosa liquid are added for the last time, and treating the insect sand to obtain the organic fertilizer containing indoleacetic acid. According to the invention, by introducing chlorella, the generation of indoleacetic acid in pig manure insect sand treated by hermetia illucens is promoted, meanwhile, the content of nitrogen, phosphorus and potassium elements in the insect sand is increased, and a solid foundation is laid for the use effect of plant fertilizers of the insect sand.

The invention relates to a UDP sugar pyrophosphorylase CpUSP gene in the field of bioengineering and application of the UDP sugar pyrophosphorylase CpUSP gene in promoting harvesting and crushing of microalgae. The nucleotide sequence of the CpUSP gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the CpUSP gene is as shown in SEQ ID NO: 2. After the UDP-sugar pyrophosphorylase of the chlorella pyrenoidosa is over-expressed in the chlamydomonas, the cell morphology can be changed, the harvesting and breaking efficiency of the chlamydomonas is remarkably improved, and a new technical scheme is provided for reducing the harvesting and cell breaking cost of the microalgae.

The invention belongs to the technical field of algae development experiments, and particularly relates to a method for measuring energy metabolism of chlorella mitochondrial stress cells. According to the method, an Agilent cell energy meter XF24 is utilized, the setting of an Agilent default experimental program is optimized, and a mixing part in all reading programs is canceled; the drug concentrations of the three targeting drugs added into the mitochondrial electron transport chain are designed through a large number of research tests, and the number of measurement cycles (5-6 times) after the drugs are added is increased, so that the oxygen consumption rate of cells is measured to obtain key parameters reflecting the mitochondrial function, and the measurement result is more accurate. According to the method, a chlorella mitochondrial pressure curve graph is given, and a new research direction is provided for energy metabolism of algae cells.

The invention discloses a plant lactobacillus and an application of the plant lactobacillus in a chlorella pyrenoidosa fermented beverage. According to the invention, a strain of phytobacterium plantarum is separated and screened from an outdoor chlorella pyrenoidosa culture pond, is named as phytobacterium plantarum HN3, and is preserved in Guangdong Microbial Culture Collection Center on the fifth floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City on July 26, 2024, and the preservation number is GDMCC No: 64816. The plant lactobacillus HN3 has good fermentation performance, acid tolerance and cholate tolerance, can degrade unpleasant odor substances of chlorella pyrenoidosa liquid to generate new pleasant odor substances, and can be used as a production strain of a chlorella pyrenoidosa fermented beverage.

The invention discloses a method for screening a chlorella pyrenoidosa mutant, which comprises the following steps: carrying out activation, culture and mutagenesis on chlorella pyrenoidosa to obtain a chlorella pyrenoidosa solution; wrapping the mutagenized algae liquid in micro-droplets, and collecting the micro-droplets in a conduit; culturing the wrapped micro-droplets under a given condition; and detecting a fluorescence signal of the cultured micro-droplet, and screening out chlorella pyrenoidosa with fast and slow growth rate based on the intensity of the fluorescence signal. The method has the beneficial effects of high flux, high precision and the like, and overcomes the defects of low flux and high false positive of the traditional method.

The invention provides a method for promoting rapid heterotrophic growth of microalgae cells. Comprising the following steps: S1, purification: separating and purifying microalgae cells by a plate streaking method to obtain a single algal colony; a nitrogen source, a carbon source and a growth stimulant are added into a culture medium, the single algae colony in the step S1 is inoculated for culture, and the growth stimulant comprises one or two of a yeast extract and a soy sauce residue extract. The growth stimulant can promote rapid heterotrophic growth of chlorella pyrenoidosa, shorten the culture period and improve the conversion rate of glucose to microalgae biomass.

The invention discloses a microbial remediation agent for heavy metal remediation as well as a preparation method and application of the microbial remediation agent. The microbial remediation agent is prepared by loading pseudomonas aeruginosa onto a carrier, the carrier comprises the following components in parts by weight: 8-22 parts of chlorella pyrenoidosa solution, 1-3 parts of silicon fertilizer and 1-3 parts of nano zero-valent iron; the silicon fertilizer is obtained by mixing granite stone powder, marble powder and an alkali activator according to the mass ratio of (8-12): (0-12): (0.5-4) and then calcining; the alkali activator is solid sodium hydroxide or potassium hydroxide. The microbial remediation agent disclosed by the invention is high in activity, rapid in reproduction, environment-friendly, pollution-free and low in cost; the form of the heavy metal can be changed, and the high-toxicity form of the heavy metal is converted into the low-toxicity form, so that the toxicity and mobility of the heavy metal are reduced, and the pollution caused by the heavy metal is remarkably reduced.

The invention provides a preparation method of mulberry and macadamia nut plant-based fermented yoghurt, and belongs to the technical field of food microbial fermentation. Mulberries are sequentially subjected to pretreatment, pulping, boiling and cooling, and mulberry pulp is obtained; sequentially baking, pulping, filtering and heating the macadamia nuts to obtain macadamia nut milk; mulberry pulp, macadamia nut milk, chlorella pyrenoidosa, cane sugar, pectin and propylene glycol alginate are mixed and homogenized, and a fermentation substrate is obtained; the mulberry and macadamia nut plant-based fermented yoghurt is obtained by sequentially sterilizing, cooling, fermenting, refrigerating and after-ripening the fermentation substrate, and the yoghurt has a relatively good antioxidant function, better conforms to the modern nutrition and health concept and is also more suitable for being absorbed by a human body; the prepared plant-based yoghurt integrates the fine taste of macadamia nuts, high-protein nutritional ingredients, the fruity flavor of mulberries and the unique flavor of chlorella pyrenoidosa, not only has better taste and flavor, but also has better functionality.

The application belongs to the technical field of water disinfection, and particularly relates to application of microalgae in killing Escherichia coli in wastewater, wherein the microalgae are one or more of Chlorella vulgaris Pediastrum ) and Chlorella pyrenoidosa Chlorella ). The application discloses for the first time that the pH and dissolved oxygen of the metabolism of the microalgae can synergistically inactivate the Escherichia coli, and play a disinfection role. In practical application, the removal rate of the Escherichia coli can reach 100%, the removal of pollutants such as TP and TN meets the discharge standard, the microalgae can independently treat the contaminated water, the removal of the pollutants does not need to be performed in advance through the conventional activated sludge method, and additional structures are not needed to kill the microorganisms in the water, so that the construction cost and operation cost are saved.

The invention discloses application of chlorella pyrenoidosa as a feed additive in Hu sheep feed preparation and breeding, and relates to the technical field of ruminant nutrition and feed. The invention provides a ruminant animal feed based on chlorella pyrenoidosa and with chlorella pyrenoidosa powder as a feed additive (the content is 1-5%). The chlorella pyrenoidosa powder contains more than or equal to 55% of crude protein and more than or equal to 25% of polyunsaturated fatty acid; the used basic ration comprises more than or equal to 14% of crude protein, more than or equal to 15.0% of crude fiber, more than or equal to 16.0% of crude ash, more than or equal to 0.50% of calcium, more than or equal to 0.40% of total phosphorus, 0.30-2.00% of sodium chloride, more than or equal to 0.40% of lysine and more than or equal to 14.0% of water. The traditional Chinese medicine composition has the effects of improving lipid metabolism and reducing deposition of saturated fatty acid for Hu sheep; the oxidation resistance and the immunity are enhanced; the meat color index is optimized, and the commodity value is improved. In addition, the feed has the advantages of reducing feed cost, adapting to efficient utilization of desert resources and the like.

The invention provides a nutritional supplement for preventing hypertension, hyperlipidemia, hyperglycemia, hyperglycemia, tumors and obesity and improving immunity and a tablet and a preparation method thereof, and belongs to the field of healthy food processing. On one hand, the invention provides the nutritional supplement for preventing three highs, tumors and obesity and improving immunity, and the nutritional supplement comprises the following raw materials: chlorella pyrenoidosa, xylooligosaccharide, yeast beta-glucan and tea leaf theanine; on the second aspect, the invention also provides a preparation method of a tablet of the nutritional supplement for preventing hypertension, hyperglycemia, hyperlipidemia, tumors and obesity and improving immunity; the invention further provides application of the nutritional supplement or tablets of the nutritional supplement in preparation of food or health food for preventing hypertension, hyperglycemia and hyperlipidemia and / or tumors and / or obesity and / or improving immunity. Compared with the prior art, the nutritional supplement for preventing hypertension, hyperglycemia, hyperlipidemia, tumor and obesity and improving immunity has remarkable improvement in the aspects of preventing hypertension, hyperlipidemia, tumor and obesity and improving immunity indexes.

The invention relates to the technical field of food, in particular to microalgae emulsion and a preparation method thereof. The microalgae emulsion provided by the invention comprises chlorella pyrenoidosa subjected to green removal; and a liquid phase for dispersing the chlorella pyrenoidosa, wherein the liquid phase consists of water and corn oil. The chlorophyll content of the chlorella pyrenoidosa subjected to green removal is extremely low, meanwhile, the protein content of the chlorella pyrenoidosa subjected to green removal is extremely high, and the formed microalgae emulsion can be milky white, so that the adverse effect of conventional green chlorella on food sense organs can be reduced, and meanwhile, the green chlorella pyrenoidosa subjected to green removal is prepared based on the effect of a liquid phase composed of water and corn oil and the chlorella pyrenoidosa subjected to green removal. The microalgae emulsion disclosed by the invention also has good stability; therefore, the microalgae emulsion disclosed by the invention has very good stability and consumer acceptability, and has a very good application prospect in the field of foods.