202 results about "Chlorella pyrenoidosa" patented technology

The invention discloses an alga ferment healthcare solid drink. The alga ferment healthcare solid drink is prepared from the following raw materials in percentage by weight: 5-13% of spirulina platensis, 8-10% of spirulina maxima, 5-15% of chlorella pyrenoidosa powder, 10-12% of haematococcus pluvialis, 10-13% of euglenophyta, 10% of alpha-amylase, 5% of lipase, 10% of cellulase, 5% of protease and 15% of fructo-oligosaccharides. A preparation process of the alga ferment healthcare solid drink comprises the following steps: (1) selecting and baking the materials; (2) drying; (3) adding the fructo-oligosaccharides; (4) crushing, purifying and sterilizing; (5) mixing; (6) carrying out incense enhancing and equalization treatment; (7) packaging. Compared with the prior art, the alga ferment healthcare solid drink has the advantages that natural raw materials serve as effective ingredients, and the intake and absorption of villi are controlled in the intestinal tract; the metabolic balance of the intestinal tract is regulated, and the value of uric acid in blood can be more effectively reduced; gout is prevented and improved, the propagation of beneficial bacteria of the intestinal tract is improved, the breeding of miscellaneous bacteria of the intestinal tract is inhibited, and the content of oxygen in the blood is increased; the immune system is regulated, and the immunity is enhanced.

The invention discloses a mixed type algae water purifying agent and application thereof. The mixed type algae water purifying agent is composed of, by weight part, 3-7 parts of chlorella pyrenoidosa and 5-10 parts of anabaena. A preparation method of the mixed type algae water purifying agent is composed of preparation of the chlorella pyrenoidosa, preparation of the chlorella pyrenoidosa and algae cell immobilization. The mixed type algae water purifying agent has the advantage that the obtained mixed type algae water purifying agent is immobilized algae cells, which is high in metabolic activity, slow in aging and capable of effectively removing ammonia nitrogen, nitrate and nitrite in water and improving the water quality environment. The mixed type algae water purifying agent is simple in process and method, high in product quality and stability, low in price and good in application effects and economic benefits.

The invention discloses an active micro-algae nutritional restoration solution, which belongs to the technical field of agriculture. The active micro-algae nutritional restoration solution is characterized by comprising multiple micro-algae at a living state, wherein the micro-algae comprises chlorella pyrenoidosa and anabaena azotica; by calculating according to the quantity of micro-algae cells,a ratio of the chlorella pyrenoidosa to the anabaena azotica is 1: (0.2 to 5). The invention also discloses a preparation method of the active micro-algae nutritional restoration solution. The preparation method of the active micro-algae nutritional restoration solution comprises the following steps: S1, separately mass cultivating the chlorella pyrenoidosa and anabaena azotica, and obtaining twoalgae solutions; and S2, mixing the two algae solutions, and obtaining the active micro-algae nutritional restoration solution. The active micro-algae nutritional restoration solution has the effectsof restoring the soil, supplementing the natural nitrogen element, activating the soil trace elements, improving the stress resistance of plants, preventing diseases and insects, and improving the quality and yield of agricultural products.

The invention discloses a method for cultivating epinephelus septemfasciatus offspring seeds, which comprises the steps of: culturing the offspring seeds under the conditions that the salinity is 28+/-2, the water temperature is 19+/-2 DEG C, the pH value is 8+/-0.3 and the dissolved oxygen is more than 6mg/l, controlling the seedling distributing density to be 8000-15000/M<3>; before newly hatched larvae enter a pool, putting a composite bacterium (photosynthetic bacterium and nitrobacterium) according to the concentration of 2ppm, adding 0.5ppm of composite bacterium and 1ppm of shrimp feedevery day after 5 days, adding 1ppm of composite bacterium and 1ppm of shrimp feed every day after 25 days, and stopping adding the composite bacterium and the shrimp feed after 50 days; illuminatingthe newly hatched larvae at 2000-4000lx until the newly hatched larvae grow to the 10th day, illuminating the epinephelus septemfasciatus growing from the 11th day to the 30th day at 1000-3000lx and illuminating the epinephelus septemfasciatus growing from the 31st day to the 60th day at 500-1000lx; after the newly hatched larvae enter the pool, sucking and removing pollutants at the bottom of the pool at the beginning of the 30th day; feeding the newly hatched larvae growing from the 4th day to the 7th day SS (Som Somatostatin) type wheel animalcule and hardly developing before the wheel animalcule is fed to the larvae, wherein larva feed comprises the SS small wheel animalcule and common wheel animalcule; and feeding the common wheel animalcule in the newly hatched larvae growing from the 6th day to the 20th day, and developing newly hatched larvae through chlorella pyrenoidosa. The invention has the advantages of reasonable operation process and stable result, and is suitable for culturing cold temperate epinephelus septemfasciatus seeds.

The invention discloses a method for culturing chlorella pyrenoidosa by taking sheep manure leach liquor as a main nitrogen source, and belongs to the field of aquiculture. The chlorella pyrenoidosa is rich in nutrition, widely used for manufacturing healthcare products, cosmetics, medicines, sea fishes and shrimp feeds; a f/2 culture solution and an open cement pit are conventionally adopted to culture, so that the growth is slow, the pollution is easily produced, the cost is high and the benefits are low; at present, a horizontal type white plastic water storage tank is adopted for aerated feeding culture, and the sheep manure leach liquor is taken as the main nitrogen source and added with an appropriate amount of nutritional ingredients such as ammonium nitrate, sodium dihydrogen phosphate, soil extract, DL malic acid, D-sodium erythorbate, potassium silicate, indoleacetic acid, alpha-naphthylacetic acid, medical stone powder, a titanium solution, wheat gluten and hydroxypropyl methyl cellulose, so that not only can the waste be utilized, but also microalgae with a high healthcare value can be obtained; the organic fertilizer and the inorganic fertilizer are mixed for use, so that the nutrition is relatively comprehensive, the operation is convenient, the automatic and large-scale production can be realized, the cost is low, the benefits are high, and the microalgae yield is increased by 240%.

The invention discloses a treatment method of aquacultural waste water. According to the treatment method, three kinds of algae, including chlorella pyrenoidosa, scenedesmus obliquus, and pseudokirchneriella subcapitata, are used for treating aquacultural waste water. According to the treatment method, a mixture of the three kings of algae is capable of removing substances in different forms, such as nitrogen and phosphor, in waste water; the treatment method is simple and effective, is low in cost, and is high in practicality; and the algae can be taken as food of aquatic animals.

The invention discloses a composite algae fungicide for improving water quality of an aquaculture pond. The composite algae fungicide is prepared from the following raw materials in parts by weight: 15-25 parts of chlorella pyrenoidosa solution, 30-50 parts of selenastrum capricornutum solution, 15-25 parts of scendesmus quadricauda solution, 5-15 parts of photosynthetic bacteria fermented liquid, 10-20 parts of sphaeailus nalans fermented liquid, 10-20 parts of thiobacillus denitrificans fermented liquid, 5-15 parts of bacillus natto fermented liquid, 5-15 parts of saccharomycetes fermented liquid and 1-2 parts of sodium humate. In addition, the invention further provides a preparation method of the composite algae fungicide for improving water quality of the aquaculture pond. After the composite algae fungicide disclosed by the invention is applied to a water body, organic pollutants in the water are transferred, transformed and degraded by utilizing metabolic activities of microbes, the pollutant concentration in the water is reduced, synergistic interaction can be realized, harmful substances such as ammonia nitrogen, nitrous acid, hydrogen sulfide and the like are decomposed, and the water body environment of the aquaculture pond is improved.

The invention discloses an active microalgae cell based nutrient repair solution, and belongs to the technical field of agriculture. The technical scheme is characterized in that the nutrient repair solution comprises a plurality of microalgae in a living state. According to the number of microalgae cells, the added microalgae comprise 5-40 parts of Tolypothrix tenuis, 30-80 parts of Chlorella pyrenoidosa and 10-50 parts of Anabaena azotica. The invention further discloses a culture method of the active microalgae cell based nutrient repair solution. The culture method comprises the followingsteps: S1, expanding and culturing Tolypothrix tenuis, Chlorella pyrenoidosa and Anabaena azotica respectively to obtain three algae solutions; S2, mixing the three algae solutions to obtain the active microalgae cell based nutrient repair solution. The active microalgae cell based nutrient repair solution has the effects of repairing soil, supplementing natural nitrogen, activating soil trace elements, improving plant stress resistance, preventing diseases and insect pests, improving quality of agricultural products and increasing yield of the agricultural products.

The invention belongs to the technical field of the sewage treatment, and discloses a method for treating a black and odorous water body by using an airlift circulation algae bio-membrane reactor. Themethod comprises the following steps: enabling chlorella pyrenoidosa to be inoculated on a bio-cord carrier for performing biofilm culturing, importing the black and odorous water body from a water inlet to a cylinder of the reactor, and opening a light source and an aerating device, lifting air and dividing the reactor to a flow rising area and a flow reducing area through a flow guide cylinder,enabling the bio-cord carrier to be positioned in the flow rising area, removing nitrogen and phosphorus pollutants in the black and odorous water body through a mixing nutrition growth reaction by the chlorella pyrenoidosa on the bio-cord carrier, after that, closing the light source and the aerating device, stewing, finally discharging treated water from a water outlet. The method has the advantages of low operation cost, good effluent quality, easy harvesting of algae and the like, and has larger application potential in the aspect of treating the black and odorous water body.

The invention discloses microalgae composite living bait for shellfish breeding and a production method of microalgae composite living bait. The microalgae composite living bait is prepared by cultivating the following microalgae species to obtain a mixed microalgae solution according to the proportion of cell numbers: 15-20% of chaetoceros muelleri, 20-25% of coccolithophores, 5-10% of isochrysis galbana, 15-20% of haematococcus pluvialis, 25-30% of chlorella pyrenoidosa and 10-15% of platymanas subcordiformis, wherein the total density is (1*105) to (5*105) cells/mL. The production method comprises the following steps: (1) utilizing photobioreactors to cultivate the microalgae species respectively, thereby obtaining the microalgae solution of which the cultivation density is (1*109) to (5*1010) cells/mL; (2) preparing the microalgae solution into the microalgae composite living bait according to the proportion of cell numbers, wherein the total density of the microalgae composite living bait is (1*105) to (5*105) cells/mL. The microalgae composite living bait is prepared from various microalgae cells, so that a nutrition balance effect is realized, the survival rate of juveniles shellfish can be effectively improved, and the morbidity of the juveniles shellfish can be reduced.

The invention discloses a method for extracting bioactive substance by breaking the walls of chlorella pyrenoidosa chick. The method comprises the following steps of: A, freezing the grout of chlorella pyrenoidosa chick live body at the temperature of between 20 and 40 DEG C, placing frozen blocks obtained by freezing in a container, and quickly extruding the frozen blocks by a physical extrusion method to ensure that the frozen blocks are broken and dissolved instantaneously; B, placing the wall breaking liquid obtained by extrusion in a stirrer for stirring; and C, performing enrichment on the wall breaking liquid by membrane separation technology at the temperature of between 0 and 5 DEG C to obtain the bioactive substance. In the method, the chlorella pyrenoidosa chick live body is frozen to ensure that cytosol in the chlorella pyrenoidosa chick cells extrudes cell walls, and then the frozen blocks are quickly broken by the physical extrusion technology, so that the cell walls of chlorella pyrenoidosa chick are fully broken by combining the two means to allow the cytosol in the cell walls to flows out; therefore, the bioactive substance in the cytosol of the chlorella pyrenoidosa chick can be effectively extracted.

The invention discloses an acaiberry health care product for Yin-deficiency constitution. The acaiberry health care product comprises the following raw materials in parts by mass: 1-6 parts of acaiberry, 1-8 parts of chlorella pyrenoidosa, 1-8 parts of loquat leaf, 1-9 parts of the fruit of Chinese wolfberry, 1-10 parts of lily, 1-10 parts of radix polygonati officinalis, 1-12 parts of rhizoma polygonati, 1-5 parts of fingered citron, 1-6 parts of lotus seed, 1-9 parts of spina date seed, 1-5 parts of raspberry, 1-5 parts of emblic leafflower fruit, 1-10 parts of mulberry, 1-6 parts of honey, 0.5-5 parts of cordyceps militaris, 1-5 parts of folium mori, 1-6 parts of fructus alpiniae oxyphyllae, 1-6 parts of the root of kudzu vine, 1-7 parts of horseradish tree leaves and 1-5 parts of Gynura procumbens, and also can comprise 1-9 parts of pawpaw. According to the holism concept of traditional Chinese medicine, yin and yang equilibrium theory and nine constitutions of the human body, the acaiberry serves as a main raw material, the pharmaceutical/food resource raw materials and new food raw materials such as chlorella pyrenoidosa, the fruit of Chinese wolfberry, loquat leaf, lily and radix polygonati officinalis are particularly and elaborately added, and the optimal compatible raw materials are elaborately and scientifically searched, so that the raw materials are complementary with one another, and healthy relation and unique advantages of dietary therapy, herbal nourishment, health maintenance and longevity are fully exerted.

The invention provides a culture method facilitating the increase of the growth rate of chlorella pyrenoidosa and cell density and the promotion of fat accumulation. Mixed bacterial fermentation liquid with inactivated Rhodobacter sp. and Rhodopseudomonas sp. is added in a culture medium for culturing chlorella pyrenoidosa. Through the adoption of the culture method, chlorella pyrenoidosa cells can grow quickly, and cultured chlorella pyrenoidosa is high in cell intensity; in particular, by adding the mixed bacterial liquid, the quick multiplication of the chlorella pyrenoidosa cells is promoted, the concentration of the chlorella pyrenoidosa cells is increased, the content of fat is obviously increased, and the method is suitable for mass and large-scale culture production.

The invention relates to a preparation method for an algae solution used for determining the chlorophyll content through a fluorescence method. The preparation method includes the steps that a chlorella pyrenoidosa species is selected, a BG-11 culture medium is prepared, sterilized, cooled and inoculated with the algae species under the aseptic condition, after inoculation is completed, the culture medium is placed in a constant-temperature illumination incubator to culture the algae species, and before the culture medium is sterilized, agar powder is added into the BG-11 culture medium to culture chlorella pyrenoidosa. According to the gelling property and stability of the agar powder, the preparation method for the algae solution is provided, the agar powder (0.08-0.10 g/100ml) is added into the BG-11 culture medium to culture the chlorella pyrenoidosa as a suspending agent, the cultured chlorella pyrenoidosa is used for preparing the algae solution, the suspension property and stability of the algae solution are improved, and then the result accuracy of determining the chlorophyll content through the fluorescence method is ensured.

The invention relates to feed for Penaeus vannamei Boone in the mature period and a preparation method of the feed. The feed comprises 25-30 parts of white fish meal, 5-10 parts of domestic fish meal, 2-5 parts of artemia dry powder, 2-5 parts of blood worm powder, 2-5 parts of earthworm powder, 10-15 parts of vital gluten, 2-5 parts of sunflower seed meal, 5-10 parts of carrot dry powder, 5-10 parts of ulva powder, 10-15 parts of haematococcus pluvialis powder, 2-5 parts of chlorella pyrenoidosa powder, 2-5 parts of cylindrotheca powder, 2-5 parts of diatom powder, 5-10 parts of cod liver oil, 2-5 parts of sea buckthorn seed oil, 0.002-0.005 part of photosynthetic bacterium, 0.002-0.005 part of bacillus subtilis and 0.01-0.05 part of composite multi-vitamin mineral. The feed has the advantages that the feed scientifically combines animal-source and plant-source feed raw materials according to the ingestion features of the Penaeus vannamei Boone and can provide sufficient nutrition for the Penaeus vannamei Boone, increase the disease resistance of the Penaeus vannamei Boone and promote molting and growth.

The invention discloses a microplate analysis method for the time toxicity of environmental pollutants on the basis of chlorella pyrenoidosa. The microplate analysis method comprises the following steps: with chlorella pyrenoidosa in freshwater green algae as a tested organism and a 96-hole transparent microplate as a carrier, introducing a time factor into a traditional compound concentration-effect relation, determining the time dependent toxicity of partial environmental pollutants on the chlorella pyrenoidosa, thereby discovering that all the determined pollutants have obvious time dependent characteristic, and then establishing a concentration-time-effect curve surface by utilizing a least-square spline function approximation method, thereby knowing the dynamic toxicity effect of the pollutants along with change of the concentration and the time. The microplate analysis method disclosed by the invention has the advantages that the defect that a traditional toxicity analysis method can only analyze the accumulation effect of pollutants for exposed organisms at a certain exposure time endpoint is overcome, the toxicity effect of the pollutants can be learned comprehensively, the mechanism and the means of the toxicological effect of the pollutants can be known deeply, and further, the phenomenon and information of exposure of the pollutants in actual environments can be embodied.

The invention discloses a plant growth regulator for promoting the growth of water plants and beneficial algae in shrimp and crab ponds and relates to the field of aquaculture. The water plants comprise waterweed. The beneficial algae comprise chlorella pyrenoidosa and scenedesmus obliquus. The plant growth regulator is a mixture comprising any one or more of diethyl aminoethyl hexanoate, compound nitrophenol, gibberellin and brassinolide. The use amount of the plant growth regulator used to promote the growth of the beneficial algae is in a range of 0.01 to 1 mg/L, and the use amount of the plant growth regulator for promoting the growth of the waterweed is in a range of 0.001 to 0.06 mg/L. The plant growth regulator can effectively promote the increasing of fresh weight of the waterweed, plant height and the growth of fibril, can observably promote the growth of chlorella pyrenoidosa and scenedesmus obliquus, can increase the density of chlorella pyrenoidosa and scenedesmus obliquus in valid time, and can improve the survival rate and growth rate of juvenile fish, shrimps and crabs.

The invention discloses a composite for improving the immunity and application. The composite is prepared from, by weight, 10-30 parts of agaricus blazei murill extract, 10-30 parts of grifola frondosa extract, 10-30 parts of chlorella pyrenoidosa, 5-15 parts of cordyceps militaris extract, 1-10 parts of ginseng extract, 10-20 parts of sea buokthom powder, 1-10 parts of yeast beta-glucose, 1-10 parts of erythritol and 1-5 parts of nutritional yeast powder. It is proved through animal tests that all the raw materials in the composite have an effect on improving the immunity synergistically.

The invention discloses a glyphosate concentration detection method based on Raman signals of chlorella pyrenoidosa. The glyphosate concentration detection method comprises the following steps: (1) acquiring Raman spectrum information of chlorella pyrenoidosa samples from water bodies with glyphosate of different concentration, and preprocessing the Raman spectrum information; (2) establishing a prediction model by virtue of alternating least square according to the concentration of glyphosate in the water bodies and the preprocessed Raman spectrum information; and (3) acquiring Raman spectrum information of a to-be-measured chlorella pyrenoidosa sample, processing the Raman spectrum information of the to-be-measured chlorella pyrenoidosa sample by virtue of a multivariate curve resolution method, and inputting the processed Raman spectrum information into the prediction model, thereby obtaining the concentration of glyphosate in a glyphosate water body from which the to-be-detected chlorella pyrenoidosa sample is collected. According to the detection method, the water sample does not need to be subjected to complicated preprocessing and chemical analysis, so that the operation steps are greatly simplified, and the detection time is shortened.