56 results about "Chlorella pyrenoidosa" patented technology

The invention discloses an EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and an application thereof. The preparation method comprises the following steps: mutagenizing a chlorella pyrenoidosa mutant strain CX41 by adopting an ethyl methanesulfonate (EMS) and normal-pressure and room-temperature plasma (ARTP) composite technology, screening and verifying to obtain a golden yellow chlorella pyrenoidosa mutant strain, naming the golden yellow chlorella pyrenoidosa mutant strain as chlorella pyrenoidosa Y11, and screening and verifying the golden yellow chlorella pyrenoidosa Y11 to obtain the chlorella pyrenoidosa Y11. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou on October 23, 2024, and the preservation number is GDMCC No: 65324. The chlorella pyrenoidosa mutant strain Y11 has the characteristics of high yield of protein and rich carotenoids, and can be used for fermentation production of microalgae protein and carotenoids.

The invention discloses a chlorella pyrenoidosa white mutant strain FZU65-W1 and application of the chlorella pyrenoidosa white mutant The classification name of the chlorella pyrenoidosa white mutant strain FZU65-W1 is chlorella pyrenoidosa, the chlorella pyrenoidosa white mutant strain is preserved in the China General Microbiological Culture Collection Center on September 11, 2024, the preservation number is CGMCC N0.46151, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with an original strain FZU65, the chlorella pyrenoidosa white mutant strain FZU65-W1 has the advantages that the growth speed of algae cells under the heterotrophic condition is higher, and meanwhile, the cells are white due to the fact that chlorophyll and carotenoid are not synthesized in the cells. The chlorella pyrenoidosa white mutant strain FZU65-W1 can be used for producing high-quality protein through heterotrophic fermentation, so that not only can a later-stage protein extraction and preparation process be simplified, but also the sensory acceptance of algae powder can be improved, and a high-quality germplasm resource is provided for industrial production and application of microalgae protein.

The invention relates to the technical field of microalgae heterotrophic culture and biomass proliferation, in particular to a chlorella pyrenoidosa heterotrophic high-density proliferation method. The specific technical scheme is as follows: the heterotrophic high-density proliferation method of chlorella pyrenoidosa comprises the following steps: pre-culture and heterotrophic propagation of chlorella pyrenoidosa: inoculating a chlorella pyrenoidosa solution into a BG11 culture medium, and culturing for 3-4 days in a constant-temperature shaking table with the temperature of 25 + / -1 DEG C, the rotating speed of 200rpm and the light-dark ratio of 0h: 24h to obtain a primary culture chlorella solution; inoculating the primary culture algae liquid into a pre-sterilized and optimized heterotrophic culture medium, and carrying out expanding culture under the same culture condition for 3-4 days to obtain a secondary expanding culture algae liquid; (2) a heterotrophic high-density culture stage: regularly supplementing a nutrient culture medium containing high-concentration glucose, nitrate and phosphate by adopting an index fed-batch feeding strategy; other conditions are kept consistent during culture; and (3) an in-situ protein accumulation induction stage: when the chlorella pyrenoidosa reaches an exponential growth end stage, adding paclobutrazol into the system, and keeping other conditions consistent while keeping exponential fed-batch culture. According to the method disclosed by the invention, the biomass of the chlorella pyrenoidosa is increased by about 128.6% compared with that of the traditional batch culture, the protein content can reach 49.14%, meanwhile, the configuration of essential amino acids is improved, and the requirements of high-end biomass resource application are met. The method has the advantages of simplicity and convenience in operation and high production efficiency, and is suitable for the fields of high-quality protein extraction and related biomass production.

The invention aims to provide a chlorella pyrenoidosa peptide with an effect of preventing alcoholic liver injury, which is prepared by the following steps: crushing chlorella pyrenoidosa, mixing with water, freezing and thawing, and performing ultrasonic treatment to prepare homogenate; alkaline protease is added for enzymolysis, and then flavourzyme is added for enzymolysis; and after the enzymolysis product is adsorbed by activated carbon, the supernate is adsorbed by using an SP Sephadex C25 cation exchange medium. The amino acid sequence of the chlorella pyrenoidosa peptide is PSEQ ID NO: 1-5. The chlorella pyrenoidosa peptide provided by the invention is obtained by enzymolysis, adsorption and cation exchange chromatography technologies, and is verified by a HepG2 cell model to obviously relieve alcoholic liver injury. The raw materials are natural and safe, the process is accurate and efficient, and industrialization potential is achieved. Meanwhile, the peptide is suitable for development of functional food, health care products and medicines, and a low-toxicity and sustainable liver protection solution is provided.

The invention discloses double-layer double-color solidification type fermented milk and a preparation method thereof, and belongs to the technical field of dairy product processing. According to the preparation method of the double-layer double-color solidification type fermented milk, chlorella pyrenoidosa is added on the basis of traditional solidification type fermentation, and double-layer solidification type mixed fermentation is adopted. According to the double-layer double-color solidification type fermented milk disclosed by the invention, a coagulant and other additives do not need to be added, and a fermented product is good in texture, relatively good in solidification form and relatively small in whey precipitation by controlling a fermentation strain and the fermentation time of the fermentation strain.

The invention provides a method for regulating and controlling growth and metabolism of caproic acid bacteria by using marine active peptides in a baijiu fermentation process. According to the method, the marine active peptides are added in an anaerobic fermentation stage of baijiu preparation to regulate and control the growth and metabolism of the caproic acid bacteria; wherein the marine active peptide is chlorella pyrenoidosa peptide, schizochytrium limacinum peptide, fish albumin peptide or euphausia superba peptide. According to the method, active peptides with specific sources are added into an anaerobic culture system, so that the growth of caproic acid bacteria can be promoted, energy metabolism can be enhanced, and the activity of key enzymes such as caproic acid dehydrogenase and caproic acid synthase can be activated, and therefore, the generation amount of flavor acids such as caproic acid and butyric acid can be increased, the fermentation quality can be improved, and the flavor level and esterification precursor substance content of Baijiu can be improved.

The invention discloses a decolorized chlorella protein, a preparation method thereof and application of the decolorized chlorella protein in preparation of foaming liquid, and relates to the technical field of biology. The preparation method comprises the following steps: extracting and decolorizing chlorella protein of chlorella pyrenoidosa, and drying to obtain the decolorized chlorella protein. According to the invention, an efficient chlorella protein extraction technology suitable for industrial production is established; the chlorella protein is treated by adopting an ethanol decolorizing method, the influence of two decolorizing methods and different decolorizing times on the decolorizing effect, the protein structure and the physicochemical property is systematically evaluated, the decolorized chlorella protein foaming liquid is prepared and represented, and the application effect of the decolorized chlorella protein foaming liquid in the foamed coffee is verified. Results show that the decolorized chlorella protein provided by the invention has excellent foaming characteristic, has the advantages of high protein purity and high sensory acceptance, and provides important technical support for efficient extraction of chlorella protein and widening of application range of chlorella.

The invention provides a method for producing an extracellular polymeric substance by using an algal-bacterial symbiotic system, and belongs to the technical field of industrial biology. The method for producing the extracellular polymeric substance by utilizing the algal-bacterial symbiotic system comprises the following steps: inoculating chlorella pyrenoidosa and lactobacillus paracasei into a BG11 liquid culture medium, culturing under the conditions that the temperature is 27-29 DEG C, the rotating speed is 160-200rpm, the illumination intensity is 3800-4200LX and the illumination ratio is 24h: 0h, then respectively collecting supernate 1 and thalli, resuspending the thalli, collecting supernate 2, and carrying out freeze-drying to obtain the extracellular polymeric substance. Enabling the supernatant 1 and the supernatant 2 to pass through a filter membrane respectively, and collecting filtrate to obtain the traditional Chinese medicine composition. When the concentration of glucose in the G11 liquid culture medium is 3.8-4.2 g / L, the pH value is 4.7-5.2, and the ratio of bacteria to algae is (0.8-1.2): 1, the growth condition of the bacteria-algae symbiotic system is optimal, and when the concentration of sodium selenite is 2-4 mg / L, the yield of the extracellular polymeric substance is highest, and efficient preparation of the extracellular polymeric substance is realized.

A biochar made by a process including drying a Chlorella Pyrenoidosa feedstock for 2 to 4 hours to form a powder and mixing the powder with a carbonate salt at a weight ratio of 1:3 to 1:5. The process further includes pyrolyzing the powder and the carbonate salt in an inert atmosphere to a temperature of 500 degrees Celsius (° C.) to 800° C. at a heating rate of 5 degrees Celsius per minute (° C. / min) to 15° C. / min to form a product, sonicating the product with an acid to form a suspension, decanting the acid, washing and sonicating the product with water, and filtering and drying the product to form the biochar.

The present application relates to a kind of illumination culture methods for improving chlorella pyrenoidosa biomass, the illumination culture method includes the following steps: chlorella pyrenoidosa stock solution is inoculated in sterile liquid medium, and chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light.The present application develops a kind of culture method for chlorella pyrenoidosa, i.e.chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light, the culture method is simple to operate, easy to implement, the method can significantly improve the growth rate and biomass accumulation of chlorella pyrenoidosa, realize the high-quality large-scale production of chlorella pyrenoidosa;It can also reduce the production energy consumption per unit biomass, solves the technical problem that yield increase and consumption reduction are difficult to consider in microalgae culture.

The invention provides a skin care composition based on a mixed chlorella extract as well as a preparation method and application of the skin care composition, and belongs to the technical field of cosmetics. The chlorella essence is prepared from the following raw material components in percentage by weight: 7%-15% of a mixed chlorella extract, 4%-10% of a synergist, 12%-30% of a humectant, 2.0%-8.0% of an emulsifier, 0.3%-0.6% of a thickening agent, 0.5%-1.2% of a preservative, 0.01%-0.4% of perfume and the balance of deionized water, the mixed chlorella extract is obtained by extracting chlorella pyrenoidosa, chlorella vulgaris and chlorella ellipsosa as raw materials. The chlorella pyrenoidosa, the chlorella vulgaris and the chlorella ellipsosa are used as raw materials to prepare the mixed chlorella extract, multiple effects and synergistic effects of the mixed chlorella extract are fully exerted, and the skin moisturizing and repairing effects are remarkably improved.

The invention relates to the technical field of chlorella protein powder, and provides anti-aging and anti-fatigue chlorella pyrenoidosa protein polypeptide powder and a preparation method thereof.The anti-aging and anti-fatigue chlorella pyrenoidosa protein polypeptide powder is prepared from, by weight, 80%-90% of chlorella protein, 5%-15% of collagen and 1%-5% of oxalic acid degrading enzyme. The chlorella protein contains various minerals such as calcium, iron, zinc and selenium, and the oxalic acid degrading enzyme can degrade oxalic acid in the chlorella protein to reduce the content of oxalic acid, so that the situation that the oxalic acid is combined with the minerals such as calcium and iron to affect absorption of the minerals such as calcium and iron by a human body can be avoided, and the health-care effect is greatly improved.

A method for improving hydrogen production efficiency by using Chlorella motors, belonging to the field of bioenergy technology. The present invention is to solve the problems that after the formation of Chlorella aggregates, it is difficult to redisperse them, resulting in a decrease in the acquisition rate of external electrons by internal Chlorella, a decrease in light energy utilization rate, and a decrease in its own survival rate, etc. The method is as follows: Using Chlorella pyrenoidosa as the living motor matrix, PDA-modified Ti3C2 two-dimensional material as the main shell material, and titanium dioxide (TiO2) nanomaterial as the motor driving material, a light-driven Chlorella motor with a yin-yang structure is constructed. By using its controllable "dispersion-aggregation" cyclic behavior, the effect of "accumulating electrons for hydrogen production when dispersed - consuming electrons for efficient hydrogen production when aggregated" of Chlorella is realized, thereby improving the survival rate, nutrient utilization rate and hydrogen production efficiency. The construction of this system is simple and the process is mild. The Ti3C2 shell has a protective effect on the biological activity and function of Chlorella, and the TiO2 shell has a photocatalytic function and a protective function.

The invention relates to a UDP sugar pyrophosphorylase CpUSP gene in the field of bioengineering and application of the UDP sugar pyrophosphorylase CpUSP gene in promoting harvesting and crushing of microalgae. The nucleotide sequence of the CpUSP gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the CpUSP gene is as shown in SEQ ID NO: 2. After the UDP-sugar pyrophosphorylase of the chlorella pyrenoidosa is over-expressed in the chlamydomonas, the cell morphology can be changed, the harvesting and breaking efficiency of the chlamydomonas is remarkably improved, and a new technical scheme is provided for reducing the harvesting and cell breaking cost of the microalgae.

The invention belongs to the technical field of algae development experiments, and particularly relates to a method for measuring energy metabolism of chlorella mitochondrial stress cells. According to the method, an Agilent cell energy meter XF24 is utilized, the setting of an Agilent default experimental program is optimized, and a mixing part in all reading programs is canceled; the drug concentrations of the three targeting drugs added into the mitochondrial electron transport chain are designed through a large number of research tests, and the number of measurement cycles (5-6 times) after the drugs are added is increased, so that the oxygen consumption rate of cells is measured to obtain key parameters reflecting the mitochondrial function, and the measurement result is more accurate. According to the method, a chlorella mitochondrial pressure curve graph is given, and a new research direction is provided for energy metabolism of algae cells.

The invention discloses a method for screening a chlorella pyrenoidosa mutant, which comprises the following steps: carrying out activation, culture and mutagenesis on chlorella pyrenoidosa to obtain a chlorella pyrenoidosa solution; wrapping the mutagenized algae liquid in micro-droplets, and collecting the micro-droplets in a conduit; culturing the wrapped micro-droplets under a given condition; and detecting a fluorescence signal of the cultured micro-droplet, and screening out chlorella pyrenoidosa with fast and slow growth rate based on the intensity of the fluorescence signal. The method has the beneficial effects of high flux, high precision and the like, and overcomes the defects of low flux and high false positive of the traditional method.

The invention discloses a microbial remediation agent for heavy metal remediation as well as a preparation method and application of the microbial remediation agent. The microbial remediation agent is prepared by loading pseudomonas aeruginosa onto a carrier, the carrier comprises the following components in parts by weight: 8-22 parts of chlorella pyrenoidosa solution, 1-3 parts of silicon fertilizer and 1-3 parts of nano zero-valent iron; the silicon fertilizer is obtained by mixing granite stone powder, marble powder and an alkali activator according to the mass ratio of (8-12): (0-12): (0.5-4) and then calcining; the alkali activator is solid sodium hydroxide or potassium hydroxide. The microbial remediation agent disclosed by the invention is high in activity, rapid in reproduction, environment-friendly, pollution-free and low in cost; the form of the heavy metal can be changed, and the high-toxicity form of the heavy metal is converted into the low-toxicity form, so that the toxicity and mobility of the heavy metal are reduced, and the pollution caused by the heavy metal is remarkably reduced.

The invention provides a preparation method of mulberry and macadamia nut plant-based fermented yoghurt, and belongs to the technical field of food microbial fermentation. Mulberries are sequentially subjected to pretreatment, pulping, boiling and cooling, and mulberry pulp is obtained; sequentially baking, pulping, filtering and heating the macadamia nuts to obtain macadamia nut milk; mulberry pulp, macadamia nut milk, chlorella pyrenoidosa, cane sugar, pectin and propylene glycol alginate are mixed and homogenized, and a fermentation substrate is obtained; the mulberry and macadamia nut plant-based fermented yoghurt is obtained by sequentially sterilizing, cooling, fermenting, refrigerating and after-ripening the fermentation substrate, and the yoghurt has a relatively good antioxidant function, better conforms to the modern nutrition and health concept and is also more suitable for being absorbed by a human body; the prepared plant-based yoghurt integrates the fine taste of macadamia nuts, high-protein nutritional ingredients, the fruity flavor of mulberries and the unique flavor of chlorella pyrenoidosa, not only has better taste and flavor, but also has better functionality.

The application belongs to the technical field of water disinfection, and particularly relates to application of microalgae in killing Escherichia coli in wastewater, wherein the microalgae are one or more of Chlorella vulgaris Pediastrum ) and Chlorella pyrenoidosa Chlorella ). The application discloses for the first time that the pH and dissolved oxygen of the metabolism of the microalgae can synergistically inactivate the Escherichia coli, and play a disinfection role. In practical application, the removal rate of the Escherichia coli can reach 100%, the removal of pollutants such as TP and TN meets the discharge standard, the microalgae can independently treat the contaminated water, the removal of the pollutants does not need to be performed in advance through the conventional activated sludge method, and additional structures are not needed to kill the microorganisms in the water, so that the construction cost and operation cost are saved.

The invention discloses application of chlorella pyrenoidosa as a feed additive in Hu sheep feed preparation and breeding, and relates to the technical field of ruminant nutrition and feed. The invention provides a ruminant animal feed based on chlorella pyrenoidosa and with chlorella pyrenoidosa powder as a feed additive (the content is 1-5%). The chlorella pyrenoidosa powder contains more than or equal to 55% of crude protein and more than or equal to 25% of polyunsaturated fatty acid; the used basic ration comprises more than or equal to 14% of crude protein, more than or equal to 15.0% of crude fiber, more than or equal to 16.0% of crude ash, more than or equal to 0.50% of calcium, more than or equal to 0.40% of total phosphorus, 0.30-2.00% of sodium chloride, more than or equal to 0.40% of lysine and more than or equal to 14.0% of water. The traditional Chinese medicine composition has the effects of improving lipid metabolism and reducing deposition of saturated fatty acid for Hu sheep; the oxidation resistance and the immunity are enhanced; the meat color index is optimized, and the commodity value is improved. In addition, the feed has the advantages of reducing feed cost, adapting to efficient utilization of desert resources and the like.

The invention relates to the technical field of food, in particular to microalgae emulsion and a preparation method thereof. The microalgae emulsion provided by the invention comprises chlorella pyrenoidosa subjected to green removal; and a liquid phase for dispersing the chlorella pyrenoidosa, wherein the liquid phase consists of water and corn oil. The chlorophyll content of the chlorella pyrenoidosa subjected to green removal is extremely low, meanwhile, the protein content of the chlorella pyrenoidosa subjected to green removal is extremely high, and the formed microalgae emulsion can be milky white, so that the adverse effect of conventional green chlorella on food sense organs can be reduced, and meanwhile, the green chlorella pyrenoidosa subjected to green removal is prepared based on the effect of a liquid phase composed of water and corn oil and the chlorella pyrenoidosa subjected to green removal. The microalgae emulsion disclosed by the invention also has good stability; therefore, the microalgae emulsion disclosed by the invention has very good stability and consumer acceptability, and has a very good application prospect in the field of foods.

The invention relates to the field of microalgae breeding, in particular to a method for screening and cultivating yellow chlorella pyrenoidosa mutant strains capable of rapidly growing, which mainly comprises the following steps: marking wild type chlorella pyrenoidosa on a flat plate, selecting single algae colonies for activation culture, and culturing the single algae colonies to obtain the yellow chlorella pyrenoidosa mutant strains. The yellow chlorella pyrenoidosa capable of rapidly growing is screened and cultured, the yellow chlorella pyrenoidosa is cultured for 4-6 days under the heterotrophic condition of 25-30 DEG C, the color characterization is stable, the hereditary character of the yellow chlorella pyrenoidosa can be judged to be relatively stable, the growth rate is high, the chlorophyll content is remarkably lower than that of wild type chlorella pyrenoidosa, the biomass of the next day can reach 2.1 g / L under the condition that the optimal carbon source is 5g / L, and the yield is high. Compared with wild type chlorella pyrenoidosa, the invention has the advantages that the content of xanthophyll and biomass are higher than those of wild type chlorella pyrenoidosa, the amino acid composition is excellent, the EAA / TAA value is 44.74%, and the EAA / NEAA ratio is 0.81, so that the chlorella pyrenoidosa has the potential of replacing traditional edible resources, can be used as an optimal substitute of traditional food due to the excellent protein quality, and broadens the application market of chlorella pyrenoidosa.

The invention discloses an active desert microalgae biological fertilizer and application thereof, and belongs to the technical field of biological fertilizers. The active desert microalgae biological fertilizer is prepared from the following components in percentage by weight: 10 to 50 percent of cyanobacteria nitrogen fixation, 10 to 30 percent of microcoleus vasinatus, 10 to 50 percent of chlorella pyrenoidosa, 10 to 30 percent of Scenedesmus armatus, 10 to 50 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens and 10 to 30 percent of bacillus The microalgae in the desert microalgae biological fertilizer is in a living body state, can perform a series of life activities such as cell reproduction and metabolism, is vigorous in vitality and high in carbon fixation and nitrogen fixation efficiency, and can remarkably improve the soil fertility, provide nutrition for crop growth, prevent soil hardening and improve the stress resistance of crops.

The invention relates to the technical field of fermentation engineering, and discloses an organic wall-broken chlorella pyrenoidosa composite solid beverage and a preparation method thereof, the organic wall-broken chlorella pyrenoidosa composite solid beverage comprises the following raw materials by weight: 10-20 parts of wall-broken chlorella pyrenoidosa powder, 5-15 parts of fermentation auxiliary material dry powder, 3-8 parts of fructo-oligosaccharide, 5-10 parts of soybean protein isolate, 3-6 parts of coconut milk powder, 1-3 parts of lemon powder, and 0.001-0.002 part of selenomethionine. According to the technology, a complete technical chain is formed from raw material treatment to finished product packaging, efficient reservation of active ingredients, synergistic enhancement of functions, sensory quality optimization and application performance improvement are achieved, and the requirements for functional solid beverages in different scenes are met.

The invention provides a preparation method of a chlorella pyrenoidosa protein peptide-calcium chelate. The preparation method comprises the following steps: S1, dissolving chlorella pyrenoidosa protein in deionized water, adjusting the pH value to 2-13, stirring for 1-5 hours, centrifuging, and taking supernate to obtain a chlorella pyrenoidosa protein treatment solution; s2, adding calcium chloride into the chlorella pyrenoidosa protein treatment liquid, chelating in a water bath at 35-38 DEG C for 1.8-2.2 hours, centrifuging, adding absolute ethyl alcohol into supernate, standing, centrifuging again, collecting precipitate, and freeze-drying to obtain the chlorella pyrenoidosa protein treatment liquid. The preparation method is simple in preparation process, free of toxic and side reagents and low in energy consumption, the calcium absorption promoting effect of the prepared product is verified through animal experiments, and a solid theoretical support is provided for development and application of the chlorella pyrenoidosa protein peptide-calcium chelate as a novel bone health promoter.

The invention relates to a white chlorella pyrenoidosa mutant strain and application thereof, and belongs to the technical field of microalgae biology. The chlorella pyrenoidosa mutant strain is named as YYZM024, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.40787, and the preservation date is September 19, 2023. The mutant strain is cultured in a glucose-containing heterotrophic culture medium, and after the mutant strain is cultured under the dark condition of 25-28 DEG C for 7-10 days, the content of chlorophyll in cells is still stably maintained at a relatively low level, and the color characterization is very stable. The chlorophyll content of the mutant strain is not obviously changed after passage inoculation for six months, and dry algae powder is always milky white, so that the genetic character of the mutant strain is relatively stable, and the mutant strain can be used as an algae species for large-scale production of algae source protein or an algae powder source of food additives.

The invention provides a yellow chlorella pyrenoidosa mutant strain APMT04 and an application of the yellow chlorella pyrenoidosa mutant strain. The chlorella pyrenoidosa mutant strain APMT04 is obtained through a chemical mutagenesis method, compared with an original strain, the chlorella pyrenoidosa mutant strain APMT04 has the advantages that the growth speed of chlorella cells under the heterotrophic condition is higher, and meanwhile, the chlorophyll content of the chlorella pyrenoidosa mutant strain APMT04 is reduced by 90% or above, but the chlorella pyrenoidosa mutant strain APMT04 contains high-content xanthophyll, so that the And the extract also contains high-content protein and grease, can be used as byproducts of xanthophyll extraction, and can be used in the fields of food, feed and the like. When the chlorella pyrenoidosa mutant strain APMT04 is used for producing xanthophyll, the later-stage extraction and preparation process of xanthophyll can be simplified, the sensory acceptance of algae powder is improved, protein and grease rich in the algae powder can serve as by-products of xanthophyll extraction, and high-quality germplasm resources are provided for industrial production and application of microalgae xanthophyll.

The invention discloses a fish manure concentrated clear liquid-based vegetable-algae symbiosis method, which is characterized in that diluted fish manure concentrated clear liquid is placed in a transparent planting tank, the concentration of the diluted fish manure concentrated clear liquid is 25-50% of the concentration of the original fish manure concentrated clear liquid, chlorella pyrenoidosa is cultured in the planting tank, and meanwhile, the chlorella pyrenoidosa is cultured in the planting tank. Leaf vegetables are planted in the planting grooves through floating plates, planting cups for planting the leaf vegetables are arranged on the floating plates, and the leaf vegetables are planted in the planting cups. A culture wastewater microalgae treatment system and a vegetable water culture system are coupled to construct a vegetable-algae symbiotic system, oxygen is supplied to vegetable roots through O2 generated by microalgae photosynthesis, vegetable growth is promoted, and nutrient absorption efficiency is improved. Finally, the purpose of reducing the concentration of pollutants in the fish manure concentrated clear liquid is achieved, meanwhile, vegetables are produced, and the purpose of resource utilization of waste of the recirculating aquaculture system is achieved.

The application belongs to the technical field of soil remediation, and discloses a microalgae biological soil remediation agent and application thereof.A sustainable benign underground micro-ecosystem is constructed, and the prepared microalgae biological soil remediation agent takes a porous active carrier with positive charges as a matrix.The porous active carrier is obtained by compounding silicon dioxide on montmorillonite, and the porous active carrier is modified to obtain an amine group of a negative charge NH2 ‑ between layers, and finally loaded with chitosan cations to become a porous active carrier with positive charges, which is loaded with a large amount of Chlorella pyrenoidosa with reduced environmental sensitivity, so that it can survive and reproduce in harsh soil environments, can significantly improve soil fertility, improve crop yield and quality, and also has the effect of reducing soil heavy metal pollution; can also increase the diversity of soil microbial communities, improve soil aggregate structure, and improve the soil physical and chemical environment.

The invention discloses a method for producing high-organic-calcium microalgae single-cell protein by recycling white spirit vinasse, which comprises the following steps: by taking the white spirit vinasse as a nutrient medium and chlorella pyrenoidosa as a single-cell protein source, firstly, pretreating the white spirit vinasse and carrying out microbial acid-producing fermentation, and dissolving the organic acid generated by fermentation into low-cost inorganic calcium (calcium carbonate) in a system to obtain the high-organic-calcium microalgae single-cell protein. When the pH of the system is adjusted and the acid inhibition in the fermentation process is relieved, an available calcium source and an organic acid carbon source are provided for the chlorella pyrenoidosa; then carrying out directional domestication breeding on the chlorella pyrenoidosa to improve the tolerance, the growth efficiency and the calcium enrichment rate of the chlorella pyrenoidosa; and finally, culturing the domesticated chlorella pyrenoidosa by using a vinasse culture medium subjected to fermentation conversion to produce microalgae single-cell protein rich in organic calcium. According to the invention, the resource reutilization of food wastes is realized, the microalgae culture cost is reduced, the organic calcium content in the obtained single-cell protein product is increased (greater than or equal to 0.48%), and the single-cell protein product can be used for preparing functional foods.