Active microalgae cell based nutrient repair solution and culture method thereof

A technology of cell nutrition and microalgae cells, which is applied in the field of agriculture, can solve problems such as limiting the value of fertilizers, and achieve the effects of high microalgae activity, activation of soil trace elements, and soil restoration

Pending Publication Date: 2019-02-15
雷云飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microalgae added to biofertilizers are usually dead and can only use

Method used

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  • Active microalgae cell based nutrient repair solution and culture method thereof
  • Active microalgae cell based nutrient repair solution and culture method thereof
  • Active microalgae cell based nutrient repair solution and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A microalgae active cell nutrient repair solution, the cultivation method includes the following steps:

[0043] S1, expanding the cultivation of Monofidobacterium microella, Chlorella pyrenoidosa and Anabaena nitrogen-fixing algae respectively to obtain three kinds of algae liquids, among which, in the process of expanding cultivation,

[0044] The expanded cultivation process of the three algae liquids includes: selecting and separating a single microalgal cell under a microscope → culturing a single microalgal cell in 15 mL of medium solution for 10 days to obtain 15 mL of algal liquid → transfer to 85 mL of medium solution Continue culturing for 10 days to obtain 100mL of algae solution → inoculate into 0.9L medium solution for 10 days to obtain 1L of algae solution → inoculate into 4L medium solution for 10 days to obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 10 days to obtain 18L of algae liquid → transferred to 782L ...

Embodiment 2

[0049] The difference from Example 1 is that in the expanded cultivation of step S1,

[0050] The expanded cultivation process of the three algae liquids includes: selecting and separating a single microalgal cell under a microscope → cultivating a single microalgal cell in 15mL of medium solution for 12 days to obtain 15mL of algae liquid → transfer to 85mL of medium solution Continue culturing for 10 days to obtain 100mL of algae liquid → inoculate into 0.9L medium solution for 12 days to obtain 1L of algae liquid → inoculate into 4L medium solution for 12 days to obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 12 days to obtain 18L of algae liquid → transferred to 782L medium solution and continued to cultivate for 12 days to obtain 800L of algae liquid. At the end of the expansion of each stage above, the number of viable microalgae cells is ≥10 6 cells / mL, the number of active microalgae cells in the final obtained algae liquid...

Embodiment 3

[0053] The difference from Example 1 is that in the expanded cultivation of step S1,

[0054] The expanded cultivation process of the three algae liquids includes: selecting and separating a single microalgal cell under a microscope→cultivating a single microalgal cell in 15mL of medium solution for 15 days to obtain 15mL of algal liquid→transferring to 85mL of medium solution Continue culturing for 15 days, obtain 100mL of algae liquid → inoculate into 0.9L medium solution and continue culturing for 15 days, obtain 1L of algae liquid → transfer and inoculate into 4L medium solution for 15 days, obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 15 days to obtain 18L of algae liquid → transferred to 782L medium solution and continued to cultivate for 15 days to obtain 800L of algae liquid. At the end of the expansion of each stage above, the number of viable microalgae cells is ≥10 6 cells / mL, the number of active microalgae cells in t...

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Abstract

The invention discloses an active microalgae cell based nutrient repair solution, and belongs to the technical field of agriculture. The technical scheme is characterized in that the nutrient repair solution comprises a plurality of microalgae in a living state. According to the number of microalgae cells, the added microalgae comprise 5-40 parts of Tolypothrix tenuis, 30-80 parts of Chlorella pyrenoidosa and 10-50 parts of Anabaena azotica. The invention further discloses a culture method of the active microalgae cell based nutrient repair solution. The culture method comprises the followingsteps: S1, expanding and culturing Tolypothrix tenuis, Chlorella pyrenoidosa and Anabaena azotica respectively to obtain three algae solutions; S2, mixing the three algae solutions to obtain the active microalgae cell based nutrient repair solution. The active microalgae cell based nutrient repair solution has the effects of repairing soil, supplementing natural nitrogen, activating soil trace elements, improving plant stress resistance, preventing diseases and insect pests, improving quality of agricultural products and increasing yield of the agricultural products.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a microalgae active cell nutrient repair liquid and a cultivation method thereof. Background technique [0002] When the soil fertility level cannot meet the nutrients required for plant growth, the soil needs to be fertilized. Chemical fertilizers are currently widely used fertilizers. However, the negative effects of chemical fertilizers cannot be ignored: the raw materials for the production of chemical fertilizers contain Zn, Heavy metals such as Cu, Co, and Cr, these heavy metals are applied to the soil with chemical fertilizers, and cause the accumulation of heavy metal elements in the soil, which are absorbed into the plants through plants, affecting plant growth and the quality of agricultural products; chemical fertilizers will reduce soil microorganisms The quantity and activity of soil microorganisms, thereby reducing the transformation of organic matter in the s...

Claims

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Application Information

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IPC IPC(8): C05G1/00C05G3/04C05G3/80
CPCC05B7/00C05F11/00C05G3/80C05G5/20C05C5/02C05D3/00C05D5/00C05D9/02
Inventor 雷云飞
Owner 雷云飞
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