Method for extracting bioactive substance by breaking walls of chlorella pyrenoidosa chick

A biologically active substance, the technology of chlorella pyrenoidosa, which is applied in the direction of pharmaceutical formulations, drug combinations, plant raw materials, etc., can solve the problem of not being able to effectively extract the biologically active substances in chlorella, and achieve the effect of ensuring activity

Active Publication Date: 2011-02-23
东莞市清晨科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there is currently no method for eff

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: First, freeze the live chlorella pyrenoidose slurry at minus 30°C to obtain a frozen block, then put the frozen block into a container and squeeze the frozen block quickly with physical pressure, so that the frozen block is broken instantly. The frozen block is subjected to rapid impact, and the frozen block is heated and dissolved to obtain a broken wall liquid; the obtained wall broken liquid is put into a high-speed stirrer to fully overflow the cytosol in the cell wall of Chlorella pyrenoidosa; and then in an environment with a temperature of 2°C, The stirred wall-breaking liquid is enriched by membrane separation technology to obtain biologically active substances. The membrane separation technology is a conventional prior art, and will not be introduced in detail here.

Embodiment 2

[0012] Embodiment 2: First, freeze the live slurry of Chlorella pyrenoidosa at a temperature of minus 20°C to obtain a frozen block, then put the frozen block into a container and use the gravity of 150 kilograms of objects to quickly squeeze the frozen block to make the frozen block Broken instantly, due to the rapid impact of the frozen block, the frozen block is heated and dissolved to obtain a broken wall liquid; the obtained wall broken liquid is put into a high-speed stirrer to fully overflow the cell fluid in the cell wall of Chlorella pyrenoidosa; then at a temperature of 0°C Under the environment of environmental protection, membrane separation technology is used to enrich the broken wall solution after stirring to obtain biologically active substances.

Embodiment 3

[0013] Example 3: First, freeze the living chlorella pyrenoidose slurry at minus 40°C to obtain a frozen block, then put the frozen block into a container and quickly squeeze the frozen block with the gravity of 100 kilograms of objects to make the frozen block Instantaneous crushing and dissolution to obtain a broken wall liquid; put the broken wall liquid into a high-speed mixer to fully overflow the cell fluid in the cell wall of Chlorella pyrenoidosa; then, at a temperature of 5°C, use membrane separation technology to separate the stirred Bioactive substances are obtained by enrichment of the wall-breaking solution.

[0014] The biologically active substances obtained above can be added to medicines as additives, can also be used as medicines for rapid growth of the epidermis, and can also be used as neuromodulators and immunity regulating nutrient solutions for health care, and have very high application value.

[0015] The above-mentioned live Chlorella pyrenoidosa slur...

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PUM

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Abstract

The invention discloses a method for extracting bioactive substance by breaking the walls of chlorella pyrenoidosa chick. The method comprises the following steps of: A, freezing the grout of chlorella pyrenoidosa chick live body at the temperature of between 20 and 40 DEG C, placing frozen blocks obtained by freezing in a container, and quickly extruding the frozen blocks by a physical extrusion method to ensure that the frozen blocks are broken and dissolved instantaneously; B, placing the wall breaking liquid obtained by extrusion in a stirrer for stirring; and C, performing enrichment on the wall breaking liquid by membrane separation technology at the temperature of between 0 and 5 DEG C to obtain the bioactive substance. In the method, the chlorella pyrenoidosa chick live body is frozen to ensure that cytosol in the chlorella pyrenoidosa chick cells extrudes cell walls, and then the frozen blocks are quickly broken by the physical extrusion technology, so that the cell walls of chlorella pyrenoidosa chick are fully broken by combining the two means to allow the cytosol in the cell walls to flows out; therefore, the bioactive substance in the cytosol of the chlorella pyrenoidosa chick can be effectively extracted.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to a method for extracting biologically active substances from chlorella pyrenoidosa by breaking the wall. 【Background technique】 [0002] Chlorella is a high-energy plant, which is rich in amino acids, proteins, vitamins and other nutrients, especially the unique bioactive growth factor C.G.F (Chlorella Growth Factor), which has high utilization value. [0003] However, there is currently no method for effectively extracting biologically active substances in chlorella. 【Content of invention】 [0004] Aiming at the above-mentioned defects of the prior art, the present invention provides a method for extracting biologically active substances from Chlorella pyrenoidosa by breaking the wall, so as to extract the biologically active substances in the Chlorella pyrenoidosa. [0005] In order to solve the above technical problems, the present invention is achieved through the following t...

Claims

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Application Information

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IPC IPC(8): A61P25/00A61K36/02A61P17/00A61P37/02
CPCA61K2236/15A61K36/05A61P17/00A61P25/00A61P37/02
Inventor 汤永强
Owner 东莞市清晨科技有限公司
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