Mixed type algae water purifying agent and application thereof
A purifying agent and hybrid technology, which is applied in the field of environmental technology and biology, can solve problems such as affecting the healthy growth of aquatic organisms, deteriorating the environment of aquaculture water, and reducing the oxygen content of the water body, and achieves good product quality stability, good economic benefits, and aging. slow effect
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Embodiment 1
[0016] A mixed type algae water body purifier, the composition and parts by weight of the purifier are: 3 parts of chlorella pyrenoidosa, 5 parts of anabaena, and the preparation comprises the following steps: (1) preparation of chlorella pyrenoidosa: In an aseptic operating environment, inoculate 1 part of Chlorella pyrenoidosa species into an Erlenmeyer flask containing 20 parts of SE medium, place the Erlenmeyer flask in a biological light incubator, control the temperature at 20°C, and use cool white fluorescent lamps As a light source, control the illuminance to 1500lx, light for 8h, dark for 8h, cultivate to the logarithmic phase, the concentration is appropriate, transfer Chlorella pyrenoidosa to a centrifuge tube, set the speed to 3600r / min, centrifuge for 10min, pour the supernatant into the waste liquid bucket, and the remaining material is Chlorella pyrenoidosa cells; (2) Preparation of Anabaena: Under aseptic operation environment, 1 part of Anabaena species was ino...
Embodiment 2
[0019] A mixed algae water purification agent, the composition and parts by weight of the purification agent are: 4 parts of chlorella pyrenoidosa, 6 parts of anabaena, and the preparation includes the following steps:
[0020] 1) Preparation of Chlorella pyrenoidosa: 2 parts of Chlorella pyrenoidosa species were inoculated into Erlenmeyer flasks containing 16 parts of SE medium, and cultured at a temperature of 22°C, with light for 9 hours, no light for 9 hours, and light intensity of 1600 lx. The chlorella pyrenoidosa in the logarithmic phase was centrifuged at 3700r / min for 12min, and the supernatant was discarded to obtain the chlorella pyrenoidosa cells. The process was performed aseptically. Under this condition, the growth rate of the chlorella pyrenoidosa cells is the fastest, and the obtained chlorella pyrenoidosa cells have high yield, high activity, and strong ability to absorb ammonia nitrogen in the water body;
[0021] 2) Preparation of Anabaena: Inoculate 3 part...
Embodiment 3
[0025] A mixed algae water purification agent, the composition and parts by weight of the purification agent are: 7 parts of chlorella pyrenoidosa, 10 parts of anabaena, and the preparation includes the following steps:
[0026] 1) Preparation of Chlorella pyrenoidosa: 5 parts of Chlorella pyrenoidosa species were inoculated in an Erlenmeyer flask containing 15 parts of SE medium, and cultivated at a temperature of 24°C, 10 hours of light, 10 hours of no light, and an illumination of 1900 lx. The chlorella pyrenoidosa in the logarithmic phase was centrifuged at 3800r / min for 14min, and the supernatant was discarded to obtain the chlorella pyrenoidosa cells. The process was performed aseptically. Under this condition, the growth rate of the chlorella pyrenoidosa cells is the fastest, and the obtained chlorella pyrenoidosa cells have high yield, high activity, and strong ability to absorb ammonia nitrogen in the water body;
[0027] 2) Preparation of Anabaena: inoculate 8 parts ...
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