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Mixed type algae water purifying agent and application thereof

A purifying agent and hybrid technology, which is applied in the field of environmental technology and biology, can solve problems such as affecting the healthy growth of aquatic organisms, deteriorating the environment of aquaculture water, and reducing the oxygen content of the water body, and achieves good product quality stability, good economic benefits, and aging. slow effect

Inactive Publication Date: 2017-09-08
舟山市普陀兴海养殖优质种苗选育研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Feed residues and metabolites of cultured organisms will increase the COD of the water body; the decomposition of a large amount of feed residues, feces and other organic matter in the water body will reduce the oxygen content of the water body; and due to the ammoniation and nitrification of microorganisms in the water body, the input The nitrogen elements in the protein, amino acid and other substances in the added feed will form ammonia nitrogen, nitrate and nitrite, which will lead to the deterioration of the breeding water environment and affect the healthy growth of aquatic organisms
[0003] The prior art, such as the Chinese invention patent with the authorized announcement number CN 104671432 B, discloses a water purification agent for aquaculture, including the fermentation liquid of Bacillus amyloliquefaciens IAE, rice husk powder and calcium carbonate powder. Secondary pollution of aquaculture water and environmental water pollution caused by the exchange of aquaculture water and surrounding water in water towns, but the process of the invention is complicated and the production cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A mixed type algae water body purifier, the composition and parts by weight of the purifier are: 3 parts of chlorella pyrenoidosa, 5 parts of anabaena, and the preparation comprises the following steps: (1) preparation of chlorella pyrenoidosa: In an aseptic operating environment, inoculate 1 part of Chlorella pyrenoidosa species into an Erlenmeyer flask containing 20 parts of SE medium, place the Erlenmeyer flask in a biological light incubator, control the temperature at 20°C, and use cool white fluorescent lamps As a light source, control the illuminance to 1500lx, light for 8h, dark for 8h, cultivate to the logarithmic phase, the concentration is appropriate, transfer Chlorella pyrenoidosa to a centrifuge tube, set the speed to 3600r / min, centrifuge for 10min, pour the supernatant into the waste liquid bucket, and the remaining material is Chlorella pyrenoidosa cells; (2) Preparation of Anabaena: Under aseptic operation environment, 1 part of Anabaena species was ino...

Embodiment 2

[0019] A mixed algae water purification agent, the composition and parts by weight of the purification agent are: 4 parts of chlorella pyrenoidosa, 6 parts of anabaena, and the preparation includes the following steps:

[0020] 1) Preparation of Chlorella pyrenoidosa: 2 parts of Chlorella pyrenoidosa species were inoculated into Erlenmeyer flasks containing 16 parts of SE medium, and cultured at a temperature of 22°C, with light for 9 hours, no light for 9 hours, and light intensity of 1600 lx. The chlorella pyrenoidosa in the logarithmic phase was centrifuged at 3700r / min for 12min, and the supernatant was discarded to obtain the chlorella pyrenoidosa cells. The process was performed aseptically. Under this condition, the growth rate of the chlorella pyrenoidosa cells is the fastest, and the obtained chlorella pyrenoidosa cells have high yield, high activity, and strong ability to absorb ammonia nitrogen in the water body;

[0021] 2) Preparation of Anabaena: Inoculate 3 part...

Embodiment 3

[0025] A mixed algae water purification agent, the composition and parts by weight of the purification agent are: 7 parts of chlorella pyrenoidosa, 10 parts of anabaena, and the preparation includes the following steps:

[0026] 1) Preparation of Chlorella pyrenoidosa: 5 parts of Chlorella pyrenoidosa species were inoculated in an Erlenmeyer flask containing 15 parts of SE medium, and cultivated at a temperature of 24°C, 10 hours of light, 10 hours of no light, and an illumination of 1900 lx. The chlorella pyrenoidosa in the logarithmic phase was centrifuged at 3800r / min for 14min, and the supernatant was discarded to obtain the chlorella pyrenoidosa cells. The process was performed aseptically. Under this condition, the growth rate of the chlorella pyrenoidosa cells is the fastest, and the obtained chlorella pyrenoidosa cells have high yield, high activity, and strong ability to absorb ammonia nitrogen in the water body;

[0027] 2) Preparation of Anabaena: inoculate 8 parts ...

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Abstract

The invention discloses a mixed type algae water purifying agent and application thereof. The mixed type algae water purifying agent is composed of, by weight part, 3-7 parts of chlorella pyrenoidosa and 5-10 parts of anabaena. A preparation method of the mixed type algae water purifying agent is composed of preparation of the chlorella pyrenoidosa, preparation of the chlorella pyrenoidosa and algae cell immobilization. The mixed type algae water purifying agent has the advantage that the obtained mixed type algae water purifying agent is immobilized algae cells, which is high in metabolic activity, slow in aging and capable of effectively removing ammonia nitrogen, nitrate and nitrite in water and improving the water quality environment. The mixed type algae water purifying agent is simple in process and method, high in product quality and stability, low in price and good in application effects and economic benefits.

Description

technical field [0001] The invention relates to the field of environmental technology and biology, in particular to a mixed algae water body purifier and its application. Background technique [0002] At present, the aquaculture area in our country reaches millions of hectares. Due to the high-density aquaculture, a large amount of feed and antibiotics need to be added to the water body during the aquaculture process. Feed residues and metabolites of cultured organisms will increase the COD of the water body; the decomposition of a large amount of feed residues, feces and other organic matter in the water body will reduce the oxygen content of the water body; and due to the ammoniation and nitrification of microorganisms in the water body, the input The nitrogen elements in the protein, amino acid and other substances in the added feed will form ammonia nitrogen, nitrate and nitrite and other substances, which will lead to the deterioration of the breeding water environment ...

Claims

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Application Information

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IPC IPC(8): C02F3/32C02F101/16C02F103/20
CPCC02F3/322C02F2101/16C02F2101/163C02F2101/166C02F2103/20
Inventor 施晓峰
Owner 舟山市普陀兴海养殖优质种苗选育研究所
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