Method for improving biomass and oil fat yield of microorganisms
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A biomass and microbial technology, applied in the field of microalgae and yeast biological fermentation and cultivation, to achieve the effects of reducing energy consumption and production costs, alleviating unfavorable factors, and simple methods
Active Publication Date: 2017-05-31
SOUTH CHINA UNIV OF TECH
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Problems solved by technology
In the process of oleaginous microorganism culture, there are few studies on the mixed co-cultivation method between different types of oleaginous microorganisms to achieve high-efficiency oil production.
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Embodiment 1
[0031] A method for improving microbial biomass and oil production, comprising the steps of:
[0032] 1) Activation culture: respectively inoculate the cells of Chlorella and Rhodotorula viscosus in the medium for activation and culture to the logarithmic growth phase and serve as the seed solution;
[0033] 2) Chlorella and yeast were inoculated in different ratios (1:1-3:1) into Basal medium (yeast extract was used as N source to replace NaNO 3 ) in a shaker flask, placed in a light incubator at 28°C, 150 rpm, with a light intensity of 100 μmol m -2 the s -1 Cultivate for 7 days; wherein, the composition of Basal medium is:
[0034] 1.25g / L sodium nitrate, 1.25g / L methyl dihydrogen phosphate, 1g / L magnesium sulfate heptahydrate, 0.5g / L EDTA, 0.111g / L calcium chloride, 0.0498g / L iron sulfate heptahydrate, 0.004g / L cobalt nitrate hexahydrate, 0.0119g / L sodium molybdate dihydrate, 0.057g / L boric acid, 0.04g / L zinc sulfate heptahydrate, 7.1mg / L manganese chloride monohydrate...
Embodiment 2
[0051] A method for improving microbial biomass and oil production, comprising the steps of:
[0052] 1) Activation culture: respectively inoculate the cells of Chlorella and Candida tropicalis in the medium for activation and culture to the logarithmic growth phase and serve as the seed solution;
[0053] 2) Chlorella and Candida tropicalis were inoculated in different ratios (1:1-3:1) into Basal medium (yeast extract was used as N source to replace NaNO 3 ) in a shaker flask, placed in a light incubator at 28°C, 150 rpm, with a light intensity of 100 μmol m - 2 the s -1 Cultivate for 7 days; wherein, the composition of Basal medium is:
[0054] 1.25g / L sodium nitrate, 1.25g / L methyl dihydrogen phosphate, 1g / L magnesium sulfate heptahydrate, 0.5g / L EDTA, 0.111g / L calcium chloride, 0.0498g / L iron sulfate heptahydrate, 0.004g / L cobalt nitrate hexahydrate, 0.0119g / L sodium molybdate dihydrate, 0.057g / L boric acid, 0.04g / L zinc sulfate heptahydrate, 7.1mg / L manganese chlorid...
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Abstract
The invention discloses a method for improving the biomass and oil fat yield of microorganisms. The method comprises the following steps: (1) carrying out activated culture: respectively inoculating chlorella pyrenoidosa and rhodotorula glutinis cells into a culture medium, carrying out activated culture to a logarithmic phase, and taking a product as a seed solution; (2) respectively inoculating chlorella pyrenoidosa and rhodotorula glutinis into a shake flask according to different proportions, wherein each shake flask is internally filled with a culture medium, putting the shake flasks into a lighting shaker, and culturing; (3) respectively inoculating chlorella pyrenoidosa and rhodotorula glutinis cells into a BG11 culture medium, wherein the ratio of chlorella pyrenoidosa cell number to the rhodotorula glutinis cell number is equal to 3: 1, and the BG11 culture mediums contain 1.6g / L of yeast extract and have different glucose concentrations; putting the inoculated shake flasks into the lighting shaker, and culturing for 12 days; (4) after the culture is finished, centrifuging microbial cells, washing and carrying out freeze drying to obtain powder; the powder can be used for measuring and evaluating the dry weight concentration, total lipid content, fatty acid content and yield of the biomass. The method has the advantages that the biomass and oil fat yield of the oil-containing microorganisms are remarkably improved by utilizing a mixed culturing mode, and are 3-4 times or more than those of microorganisms cultured under a single culture condition; the method is relatively simple and convenient, and the energy consumption and the production cost are effectively reduced.
Description
technical field [0001] The invention relates to microalgae and yeast biological fermentation culture technology, in particular to a method for increasing biomass and oil production through mixed culture of chlorella pyrenoidosa and oleaginous yeast. Background technique [0002] The continuous development of social economy has brought many problems such as the depletion of fossil energy and environmental pollution. With the increasingly prominent contradiction between supply and demand of oil products, the development of renewable resources through multiple channels has become an inevitable trend. As a new type of bioenergy, the superiority of biodiesel has attracted increasing attention in recent years. Microbial oil is also called single-cell oil. Its fatty acid composition is similar to that of general vegetable oil. It is mainly saturated and unsaturated fatty acids such as C16 and C18 series fatty acids, such as palmitic acid, stearic acid, oleic acid and linoleic acid...
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