122 results about "Rhodotorula species" patented technology

The present invention provides several methods for biological production of para-hydroxycinnamic acid (PHCA). The invention is also directed to the discovery of new fungi and bacteria that possess the ability to convert cinnamate to PHCA. The invention relates to developing of a new biocatalyst for conversion of glucose to PHCA by incorporation of the wild type PAL from the yeast Rhodotorula glutinis into E. coli underlining the ability of the wildtype PAL to convert tyrosine to PHCA. The invention is also directed to developing a new biocatalyst for conversion of glucose to PHCA by incorporation of the wildtype PAL from the yeast Rhodotorula glutinis plus the plant cytochrome P-450 and the cytochrome P-450 reductase into E. coli. In yet another embodiment, the present invention provides for the developing of a new biocatalyst through mutagenesis of the wild type yeast PAL which possesses enhanced tyrosine ammonia-lyase (TAL) activity.

The invention relates to the field of microbial engineering, and discloses a method for extracting grease from oleaginous microorganisms. The method comprises the following steps of: mixing cellulose and protease to form mixed enzyme, hydrolyzing wet thalli of the oleaginous microorganisms, centrifuging, leaching an obtained precipitate, and desolventizing to obtain the grease, wherein the mass ratio of the cellulose to the protease is 1:(0.25-4); and the protease is neutral protease or alkali protease. In addition, the method also comprises the following steps of: hydrolyzing wet thalli of Rhodotorula glutinis by using the protease, centrifuging, leaching an obtained precipitate, and desolventizing to obtain the grease, wherein the protease is the neutral protease or the alkali protease;and the mass ratio of the protease and the thalli of the oleaginous microorganisms is (0.01-0.15):(1-10). By the method, the wet thalli are directly adopted for extraction, so that energy consumptionis reduced, reaction conditions are mild, the reaction has high specificity, the higher yield of the grease can be obtained with a smaller amount of enzyme, and extraction efficiency is higher; and the method is favorable for industrial production.

The present invention provides several methods for biological production of para-hydroxycinnamic acid (PHCA). The invention is also directed to the discovery of new fungi and bacteria that possess the ability to convert cinnamate to PHCA. The invention relates to developing of a new biocatalyst for conversion of glucose to PHCA by incorporation of the wild type PAL from the yeast Rhodotorula glutinis into E. coli underlining the ability of the wildtype PAL to convert tyrosine to PHCA. The invention is also directed to developing a new biocatalyst for conversion of glucose to PHCA by incorporation of the wildtype PAL from the yeast Rhodotorula glutinis plus the plant cytochrome P-450 and the cytochrome P-450 reductase into E. coli. In yet another embodiment, the present invention provides for the developing of a new biocatalyst through mutagenesis of the wild type yeast PAL which possesses enhanced tyrosine ammonia-lyase (TAL) activity.

The invention disclose a technique for the tissue culturing and breeding of the red-welcome cuckoo as well as the technique for domestication and replanting, which comprises the first generation culture of inducing the clump sprout by applying the germ-free seedling as explant, subculture for enrichment-culture, root-growing inducing for the clump sprout, water-planting for domestication before replanting the tissue culture sprout, the final replanting to flowerpot and getting the red-welcome cuckoo. The invention is suitable for the red-welcome cuckoo from different places, the k-factor of the clump sprout is high, the root is uniform, and the survival rate after the water-culture domestication is high and is suitable for the red-welcome cuckoo commercial production.

The invention discloses a method for acquiring a rhodotorula glutinis phenylalanine deaminase gene. The method comprises the following steps of: extracting RNA (Ribose Nucleic Acid); carrying out inverse transcription; carrying out PCR (Polymerase Chain Reaction) amplification; establishing a cloning vector pUCm-T-pal; and measuring a sequence to obtain a full-length phenylalanine deaminase gene sequence. The invention belongs to the technical field of gene clone. The method disclosed by the invention has the advantages of easiness for operation and higher efficiency and success rate.

The invention relates to a rhodotorula glutinis oil genetic engineering strain and a construction method and an application thereof. The construction method of the genetic engineering strain is mainly as follows: utilizing rDNA (recombinant deoxyribonucleic acid) of rhodotorula glutinis as a target sequence for homologous integration, using strong promoter genes PGK1 of saccharomyces cerevisiae and malate dehydrogenase genes ME of chaetomium cochloides to construct an expression vector to be introduced into rhodotorula glutinis, and enabling ME genes to obtain high-efficient expression in a rhodotorula glutinis body, wherein the content of lipid in a transformant is improved by 2.5 times in comparison with a wild strain. According to the construction method disclosed by the invention, key enzyme genes and a strong promoter for anabolism of the lipid are introduced on the basis that the anabolism of microbial oil is known, so that the lipid metabolism is regulated and controlled, and the yield of oil is improved. The genetic engineering strain can be applied to production of the microbial oil and development of functional oil related products, such as medicaments, health care products and the like.

The invention discloses an orange biological preserving agent based on yeast, sodium bicarbonate and elicitor activity, composed of cryptococcus laurentii suspension, rhodotorula glutinis suspension, rhodosporidium toruloides suspension, sodium bicarbonate, auxin, salicylic acid and water. The preserving agent has 1*1010-1*1012 cryptococcus laurentii cells, 1*1010-1*1012 rhodotorula glutinis cells, 1*1010-1*1012 rhodosporidium toruloides cells, 5-50 grams of sodium bicarbonate, 10-400mg of auxin, 1-100mg of salicylic acid and the reminder consisting of water per liter of the preserving agent. The accession number of cryptococcus laurentii is CGMCC No.3590, the accession number of rhodosporidium toruloides is IMI 394084, and the accession number of rhodotorula glutinis is CGMCC No.3589. The orange biological preserving agent disclosed in the invention can control orange postharvest diseases without using chemical bactericides.

The invention discloses an orange biological preservative based on antagonistic activities of three antagonistic yeasts, composed of cryptococcus laurentii suspension, rhodotorula glutinis suspension, rhodosporidium toruloides suspension and water. The preserving agent has 1*1010-1*1011 cryptococcus laurentii cells, 1*1010-1*1011 rhodotorula glutinis cells, 1*1010-1*1011 rhodosporidium toruloides cells1 and the reminder consisting of water per liter of the preserving agent. The accession number of cryptococcus laurentii is CGMCC No.3590, the accession number of rhodosporidium toruloides is IMI 394084, and the accession number of rhodotorula glutinis is CGMCC No.3589. The orange biological preserving agent disclosed in the invention can control orange postharvest diseases without using chemical bactericides.

The invention discloses a method for enhancing effect of rhodotorula glutinis on biologically controlling a postharvest disease of a fruit, comprising the following steps of: activating the rhodotorula glutinis, inoculating the rhodotorula glutinis in an NYDB culture medium added with 0.01-1 percent of chitosan to carry out induction culture, and obtaining bacteria through centrifugation, wherein the NYDB culture medium is prepared from 5g of yeast extract, 8g of beef extract, 10g of glucose and 1000ml of purified water and has natural pH; preparing 1*106/mL-1*109/mL bacterial suspension by diluting the cell with sterile water; putting fruits into the bacterial suspension, immediately taking out after soaking for 30s, and naturally air drying; putting the fruits into a plastic basket; sealing with fresh-keeping film and storing at room temperature or under cold storage condition. The invention uses the rhodotorula glutinis after induction culture by the chitosan to replace a chemical fungicide to control the postharvest disease of fruit, prevents harm of using chemical fungicide to the human body and has remarkable economic benefit and social benefit.

The invention provides a method for preventing fruit postharvest diseases, relating to prevention on fruit postharvest diseases and storing and refreshing method. Fruits are placed in microwave at 2450MHz and 0.25-0.45KW to be treated for 1-4 minutes, taken out to be placed at room temperature for 20 minutes, then immersed into 1x10<7>-1x10<9>ps/ml cryptococcus laurentii or rhodotorula glutinis solution and immediately taken out after immersion for about 30 seconds, then air drying is carried out, the fruits are placed in a plastic crate and stored under the conditions of room temperature or cold storage (20 DEG C) after sealed by preservative film. Through test, the combination of microwave and antagonism yeast can effectively prevent and treat fruit diseases caused by pathopoiesia mould, reduce fruit rotting rate and prolong fruit shelf life without destroying fruit storage quality or nutrition. The method of combining microwave treatment and bio-controlling micro-organism treatment used in the invention is simple in use, convenient in operation and low in cost, can avoid harm to human caused by using chemical disinfectant and pollution to environment and has significant economic benefit and social benefit.

The invention discloses a biological preservative for oranges, consisting of cryptococcus laurentii suspension, rhodotorula glutinis suspension, rhodosporidium suspension, salicylic acid, auxin, calcium chloride and water, wherein each liter of preservative contains 1*1010-1*1012 cryptococcus laurentii cells, 1*1010-1*1-12 rhodotorula glutinis cells, 1*1010-1*1012 rhodosporidium cells, 1-100mg of salicylic acid, 10-20mg of auxin, 5-50g of calcium chloride and the balance of water; the preservation number of the cryptococcus laurentii is CGMCC (China General Microbiological Culture Collection) NO.3590, the preservation number of the rhodosporidium is IMI (Innovative Medicines Initiative) 394084 and the preservation number of the rhodotorula glutinis is CGMCC NO.3589. The biological preservative for oranges, which is disclosed by the invention, can be used for effectively controlling postharvest diseases of orange fruits on the premise of no use of chemical bactericide.

The invention discloses a method for utilizing industrial wastes to produce microbial grease by means of fermentation and a special strain Rhodotorula glutinis Rh.g CGMCC No.2258. The method of the invention is a method for utilizing the industrial wastes (such as waste water of industrial production like starches, monosodium glutamate, paper making and so on, and/or fermentation industrial residues like soya residues and so on) as culture media to produce the microbial grease through fermentation of the Rhodotorula glutinis; the microbial grease obtained by the method also can be taken as material lipid to prepare biodiesel, and conversion rate can reach 80 to 99 percent. The production method of the microbial grease has the advantages of simple operation, low cost and high use value, and not only pollution of high concentration wastes which are discharged by fermentation industries like starches, monosodium glutamate, paper making, soya residues, etc. on the environment can be reduced but also the wastes can be converted into treasures by using the wastes to produce the grease which is a product in short supply in the current market, thereby the aim of resource utilization of the wastes is reached.

The invention relates to an intestine-regulating constipation-removing enzyme and a preparation method thereof. The enzyme comprises the following raw materials in percentage by weight: 5% of figs, 10% of sophora flowers, 15% of celery, 20% of pears, 20% of apples, 2% of radix paeoniae alba, 4% of plantain, 3% of liquorice, 20% of mulberries, 2% of dandelion, 20% of flaccid knotweed herb and 80% of coarse rice. The preparation method comprises the following steps: 1. steaming the coarse rice in the raw materials, alcoholizing the steamed coarse rice and the flaccid knotweed by hansenula anomala and saccharomyces cerevisiae, and after alcoholizing, adding water, acetifying by acetobacter pasteurianus and clostridium butyricum; 2. grinding the other materials, alcoholizing by honey yeasts, hansenula anomala, rhodotorula glutinis, saccharomyces cerevisiae and streptomyces microflavus, after finishing the steps 1 and 2, mixing according to a ratio of 1 to 1, lactifying by lactobacillus lactis and plant lactobacilli, and fermenting to obtain suspension liquid as the intestine-regulating constipation-removing enzyme. The intestine-regulating constipation-removing enzyme has the effects of promoting production of body fluid, moistening the lung and facilitating intestinal tract movement, and is suitable for crowds with constipation.

The invention relates to a new method for preparing high-activity ocean rhodotorula glutinis powder. The method comprises the following steps: beer yeast is taken as a raw material and hydrolyzed by hydrolase to be a basal medium after filtering; after ocean rhodotorula glutinis is inoculated at deep fluid for aerobic culture, and an ultrafiltration membrane is utilized in time to concentrate fermentation liquor and collect rhodotorula glutinis thallus; and the concentrated rhodotorula glutinis is mixed evenly with excipient to form wet cenobium, and then the wet cenobium are transmitted to expansion drying equipment by a screw stem for instantaneous drying at low temperature to obtain the rhodotorula glutinis dry powder of which the rhodotorula glutinis thallus reaches 10-20 billion/g, and the viable yeast content reaches above 60%. The rhodotorula glutinis prepared by the preparation method of the invention has high growth speed, high density of yeast as per unit volume and short incubation time; the ultrafiltration membrane is adopted to concentrate culture solution, which has small damage to thallus and easily keeps high activity of the concentrated rhodotorula glutinis; granulation is not needed after the concentrated rhodotorula glutinis solution and the excipient are mixed; and the obtained wet cenobium can directly enter the expansion drying equipment to for instantaneous drying at low temperature, thus ensuring high activity ratio of the rhodotorula glutinis powder in the whole production technology process.

The invention provides a method for preparing a rhodotorula glutinis feed. The method comprises the following steps of: preparing a primary fermentative strain of 5 degree Be wort by using the rhodotorula glutinis as a parent strain; inoculating the primary fermentative strain into the 5-degree Be wort in a weight ratio of 10 percent; after 12 hours, continuously feeding a nutrient source containing 2 percent of maltose, 0.2 percent of urea and 0.1 percent of ammonium sulfate in 16 hours; culturing for 48 hours, wherein the viable count of the rhodotorula glutinis in the fermentation broth is more than or equal to 109 per milliliter, and the rhodotorula glutinis is used as a secondary fermentative strain; inoculating the secondary fermentative strain accounting for 5 to 8 percent of a distiller grain fermentation medium; tiling the uniformly stirred fermentation medium in a fermenting tank to ferment at the temperature of between 28 and 30 DEG C for 60 hours, wherein the thickness of the medium is between 20 and 25cm; and drying the strain by hot wind through a feed drier at the temperature of between 50 and 60 DEG C until the moisture of the strain is lower than 5 percent, and packaging the strain to obtain the rhodotorula glutinis feed finished product.

The present invention relates to the field of microbial remediation of cadmium contamination, and discloses an application method of a microbial inoculum capable of controlling a cadmium-polluted farmland by in-situ converting, and the microbial inoculum includes Acidiphilium cryptum, Candida rugosa, Acetobacter diazotrophicus, Rhodotorula glutinis and Pseudomonas aeruginosa. Through expanding culture of the microbial inoculum, the bacteria concentration reaches more than 1 x 10<9> / mL, he microbial inoculum is sprayed directly to the soil surface of the cadmium-polluted farmland by a small-dosage multi-time multi-point method, average 14-20L of the microbial inoculum liquid is applied to per square meter of the farmland, the soil and the repair microbial inoculum are evenly mixed by turning over, and the turning-over depth is 25-35cm. The application method of the microbial inoculum has the advantages of wide application range, repeated use of the microbial inoculum, large processing capacity, simple operation and low cost, can improve the conversion efficiency of the microbial inoculum to 50% or more, and has broad application prospects in the field of heavy metal contaminated soil remediation.

The invention provides a microbial oil preparation method with biodiesel byproducts as carbon sources and belongs to the technical field of microbial oil preparation. According to the microbial oil preparation method, microbial oil is prepared with the biodiesel byproducts serving as the carbon sources and rhodotorula glutinis 32489 serving as the oil-producing microorganisms. Therefore, the problem of high cost when glucose or pure glycerine (AR) is used as the carbon sources to prepare microbial oil is solved, and crude glycerine is effectively used; the components of the produced microbial oil are almost as same as those of vegetable oil. Compared with vegetable oil, microbial oil has the advantages of being short in the preparation cycle and free of occupying arable land, is a cheap non-crop biomass raw material to prepare biodiesel, can reduce the preparation cost of biodiesel, and has a wide application prospect.

The invention discloses a biological organic fertilizer produced by using erythromycin bacterium residues and a preparation method of the biological organic fertilizer. The biological organic fertilizer is prepared from the following raw materials in parts by weight: 37-43 parts of sheep manure, 27-33 parts of the erythromycin bacterium residues, 7-13 parts of bone meal, 12-18 parts of attapulgite powder, 2-8 parts of sawdust, 0.04-0.08 part of polyglutamic acid, and 0.35-0.41 part of microbial bacteria, wherein the microbial bacteria comprise rhodotorula glutinis, high temperature fermentation bacteria and functional bacteria in a ratio of 1.8:1:1. The biological organic fertilizer is prepared by the method comprising the following processes: (1) a process of degrading the bacterium residues; (2) a process of performing fermentation to prepare a fertilizer; and (3) a process of performing crushing and screening. According to the biological organic fertilizer disclosed by the invention, abundant nutrients are obtained from the bacterium residues, and the polyglutamic acid and the bone meal are added to improve the nutrient content, so that the nutrient content is high; the polyglutamic acid and protein can be quickly absorbed and utilized by crops under the action of the functional bacteria, so that the fast-acting property is good; and the abundant nutrients are obtained from waste bacterium residues, a small amount of the bone meal and the polyglutamic acid are added to improve the nutrient content, and a high-cost chemical fertilizer is not needed to be added, so that the production cost is low.

The invention discloses a method for efficiently synthesising and extracting anthocyanin in blueberry, and specifically discloses a synthesis and extraction preparation technology for blueberry anthocyanin. The method comprises the following steps: culturing blueberry cells by high hydrostatic-pressure assisted microbial fermentation, and extracting blueberry anthocyanin by ultrafiltration chromatography; breaking the cell walls of blueberry fruits by virtue of a high hydrostatic-pressure assisted microbial metabolite to adequately release anthocyanin; adding producing enzymes of L-phenylalanine, rhodotorula glutinis and the like to promote synthesis for precursors which are not converted to anthocyanin in blueberry into anthocyanin simultaneously; subjecting the fermentation liquor to treatment of centrifuging, ultrafiltration separation, chromatographic purification, vacuum freeze-drying and the like to obtain anthocyanin with the purity of 99.31%, wherein the extraction amount of anthocyanin is increased by 15-30%. The method disclosed by the invention is simple to operate, clean and harmless in generation process, low in cost, high in anthocyanin yield, high in purity, without the need of human intestinal microbial degradation, high in medical treatment health value, suitable for large-scale production application, and extremely high in market prospect.

The invention discloses a biological preservative for cherry and tomato fruits and use thereof. Per L of biological preservative is prepared from 1*10<10>-1*10<12> rhodotorula glutinis cells, 400-600mg of rhamnolipid and the balance of water. The rhodotorula glutinis cells are rhodotorula glutinis (Rhodotorula glutinis) ZJU11; the preservation number is CGMCC NO.3589; the rhamnolipid is concentrated rhamnolipid of which the glycolipid content is 70-90%. By using the biological preservative for cherry and tomato fruits, not only can decaying of the cherry and tomato fruits be delayed, but also the quality of the cherry and tomato fruits can be ensured.

the invention discloses microbe viable bacteria combined agent and preparing method, which consists of Bacillus subtilis and Rhodotorula glutinis, wherein the Bacillus subtilis is seeded firstly and then Rhodotorula glutinis, which need 15-100 h as combined culturing time.

The invention discloses a method for culturing synthetic bacteria in rapid growth and acid production and obtained synthetic bacteria. Original strains mainly comprise Acidiphilium cryptum, Candida rugose, Acetobacter diazotrophicus, Rhodotorula glutinis and Pseudomonas aeruginosa. Pyrrhotite powder is added into a culture medium, strain inoculation quantity ranges from 5.0*10<6>/liter to 8.0*10<6>/liter, and aeration culture is performed. By the method, efficient acid production mixed bacteria can be rapidly and substantially cultured, and the pH (potential of hydrogen) of solution is reduced to be around 2.0. The bacteria are applied to rapid leaching treatment for heavy metal contaminated soil and wide in prospect.

Provided is a microbial preparation for urban garbage percolate treatment and an application thereof. The invention relates to a microbial preparation for urban sewage treatment and an application thereof. Viable bacteria in the microbial preparation comprise euryhaline bacteria bacillus cereus, Bacillus marisflavi, rhodopuesdomonas pulstris and rhodopseudomonas acidophila, Nitrosomonas europaea, marine pseudomonas and paracoccus denitrificans and yeast rhodotorula glutinis. The invention further provides a production method of the microbial preparation. According to the microbial preparation, the effect on treating garbage percolate is obvious, operation is convenient, and the microbial preparation has better application and promotion value.

A genetically engineered bacterium for synthesizing resveratrol is disclosed. The genetically engineered bacterium is obtained by knocking tyrR and trpED which are genes limiting tyrosine synthesis from glucose out of E.coli BW25113, and the chromosome is recombined with a tyrosine deaminase gene tal of rhodotorula glutinis, a petroselinum crispum p-coumaric acid: coenzyme A ligase gene 4cl and a stilbene synthase gene sts of grapes. The genetically engineered bacterium adopts glucose as a substrate to synthesize the resveratrol.