Method for enhancing effect of rhodotorula glutinis on biologically controlling postharvest disease of fruit

A technology for fruit postharvest diseases and Rhodotorula viscosum, which is applied in the fields of fruit and vegetable preservation, food science, food preservation, etc., and can solve problems affecting the effectiveness of postharvest antagonistic yeast biocontrol

Active Publication Date: 2010-12-15
无棣鑫岳化工集团有限公司
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  • Application Information

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Problems solved by technology

However, there is no relevant report at home and abroad on whether chitosan cult

Method used

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  • Method for enhancing effect of rhodotorula glutinis on biologically controlling postharvest disease of fruit
  • Method for enhancing effect of rhodotorula glutinis on biologically controlling postharvest disease of fruit
  • Method for enhancing effect of rhodotorula glutinis on biologically controlling postharvest disease of fruit

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Experimental program
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Embodiment 1

[0023] Embodiment 1: the inhibitory effect of chitosan-induced cultivation of Rhodotorula glutinosa on fruit diseases

[0024] 1. Test plan

[0025] After the fruit has been processed, wounds of uniform size and depth are formed on the equatorial part of the surface of each fruit with a sterile punch. Wherein the superficial wound of strawberry is 3mm (diameter) * 3mm (deep), the superficial wound of peach fruit is 5mm (diameter) * 3mm (deep). Add 30 μl (1) yeast suspension cultured by NYDB medium; (2) yeast suspension cultured by chitosan supplemented medium (NYDB+chitosan); (3) sterile water (as control). After 2h, an equal amount of 30 μl of pathogenic spores (B. cinerea concentration of 1×10 5 spores ml -1 ; P.expansum concentration was 5×10 4 spores / mL, the concentration of R. stolonifer was 1×10 4 spores / mL). After natural drying, the fruit is put into a plastic basket and sealed with a preservative film, cultivated in a constant temperature incubator (water activ...

Embodiment 2

[0035] Example 2: Chitosan-induced cultivation of Rhodotorula viscosus on the natural rot and storage quality of fruit

[0036] 1. Test plan

[0037] After the test fruit was picked, soak the fruit for 30s directly with the following treatments: (1) Rhodotorula viscosus suspension cultured in NYDB medium; (2) Yeast suspension cultured in chitosan-supplemented medium (NYDB+chitosan); (3) Sterile water (as a control). Then put the fruit in a plastic basket, air-dry and seal it with plastic wrap. Strawberries were stored in a 4°C freezer, and after 20 days, they were transferred to a 20°C constant temperature box for storage. After 7 days, the fruit rot was observed, and the rot rate was calculated; peaches were stored in a 4°C freezer, and after 30 days, they were transferred to 25°C. decay rate, and storage quality analysis. Each treatment was repeated 3 times, each time 10 fruits were repeated, and the whole experiment was repeated 2 times.

[0038] The storage quality ana...

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Abstract

The invention discloses a method for enhancing effect of rhodotorula glutinis on biologically controlling a postharvest disease of a fruit, comprising the following steps of: activating the rhodotorula glutinis, inoculating the rhodotorula glutinis in an NYDB culture medium added with 0.01-1 percent of chitosan to carry out induction culture, and obtaining bacteria through centrifugation, wherein the NYDB culture medium is prepared from 5g of yeast extract, 8g of beef extract, 10g of glucose and 1000ml of purified water and has natural pH; preparing 1*106/mL-1*109/mL bacterial suspension by diluting the cell with sterile water; putting fruits into the bacterial suspension, immediately taking out after soaking for 30s, and naturally air drying; putting the fruits into a plastic basket; sealing with fresh-keeping film and storing at room temperature or under cold storage condition. The invention uses the rhodotorula glutinis after induction culture by the chitosan to replace a chemical fungicide to control the postharvest disease of fruit, prevents harm of using chemical fungicide to the human body and has remarkable economic benefit and social benefit.

Description

technical field [0001] The invention relates to a Rhodotorula glutinis strain and a method for inducing and cultivating the strain with chitosan to improve the effect of the strain on post-harvest disease control of fruits. Background technique [0002] The loss of fruits due to rot in the process of picking, transportation and storage is huge, which has become a global concern. According to reports, the resulting loss in developed countries is 10%-25%, while in developing countries lacking refrigeration facilities, it is as high as 40%-50%. The postharvest loss of fruits in my country is about 20%-25%. The National Economic and Social Development Statistical Bulletin of the People's Republic of China in 2006 issued by the National Bureau of Statistics in March 2007 shows that in 2006, my country's total fruit output reached 170.5 million tons. According to this estimate, the loss of fruits after harvest in my country will reach the level of tens of billions of yuan every y...

Claims

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Application Information

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IPC IPC(8): A23B7/155
Inventor 张红印葛玲玲陈克平任晓锋
Owner 无棣鑫岳化工集团有限公司
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