Application method of microbial inoculum capable of controlling cadmium-polluted farmland by in-situ converting

A technology for microbial inoculum and cadmium pollution is applied in the field of microbial remediation of cadmium pollution, which can solve the problems of insignificant effect of microbial inoculum, inability to realize industrial application, lack of application methods, etc., and achieves efficient solution of cadmium pollution in cultivated land, low cost and suitable for use. wide range of effects

Active Publication Date: 2017-08-29
HUNAN AGRI BIOTECH RES CENT +1
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Problems solved by technology

However, the effect of microbial bacterial agents is not significant, the utilization rate is low, the waste of resources is serious, the app

Method used

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Embodiment 1

[0024] Acidiphilium cryptum ATCC33463, Candidarugosa CCTCC AY 93026, Acetobacterdiazotrophicus CGMCC No.5455, Rhodotorulaglutinis ATCC96365 and Pseudomonas aeruginosa CCTCC AB 2013184 is expanded by mixing 15%, 20%, 25%, 30%, and 10% in quantity ratio, and the basic salt medium composition of the microbial agent includes: (NH 4 ) 2 SO 4 , 0.3g / L; KCl, 0.01g / L; K 2 HPO 4 , 0.05g / L; MgSO 4 ·7H 2 O, 0.05g / L and Ca(NO 3 ) 2 , 0.001g / L. In addition, add glucose, 2g / L; yeast powder 0.01g / L; sulfur powder 0.01g / L;

[0025] The expansion method of the microbial agent is as follows: the initial bacterial concentration is 1×10 6 Individual / mL, the inoculum amount is 10%-20% (v / v), aerated culture under the condition of 16-30℃, the aeration rate is 0.16m 3 / min;

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Abstract

The present invention relates to the field of microbial remediation of cadmium contamination, and discloses an application method of a microbial inoculum capable of controlling a cadmium-polluted farmland by in-situ converting, and the microbial inoculum includes Acidiphilium cryptum, Candida rugosa, Acetobacter diazotrophicus, Rhodotorula glutinis and Pseudomonas aeruginosa. Through expanding culture of the microbial inoculum, the bacteria concentration reaches more than 1 x 10<9> / mL, he microbial inoculum is sprayed directly to the soil surface of the cadmium-polluted farmland by a small-dosage multi-time multi-point method, average 14-20L of the microbial inoculum liquid is applied to per square meter of the farmland, the soil and the repair microbial inoculum are evenly mixed by turning over, and the turning-over depth is 25-35cm. The application method of the microbial inoculum has the advantages of wide application range, repeated use of the microbial inoculum, large processing capacity, simple operation and low cost, can improve the conversion efficiency of the microbial inoculum to 50% or more, and has broad application prospects in the field of heavy metal contaminated soil remediation.

Description

technical field [0001] The invention relates to the field of microbial remediation of cadmium pollution, in particular to an efficient application method of microbial bacterial agents capable of in-situ conversion of cadmium-contaminated cultivated land. Background technique [0002] As an important part of the ecosphere, soil is one of the most basic material foundations for human survival, and it is also the main storage place for various pollutants. In my country, with the construction of industrial and agricultural production and rural urbanization, soil pollution has spread rapidly and the degree of pollution has gradually increased. [0003] Soil pollution, especially the accumulation of heavy metals in food and vegetables, puts humans at the top of the food chain at risk. In recent years, cadmium pollution has been particularly serious. There have been many reports of soil pollution caused by cadmium in my country. In the mid-1980s, a study on the polluted area caus...

Claims

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Application Information

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IPC IPC(8): C09K17/14B09C1/10C12N1/20C12N1/16C12R1/01C12R1/72C12R1/02C12R1/645C12R1/385C09K101/00
CPCB09C1/105B09C2101/00C09K17/14C09K2101/00C12N1/16C12N1/20
Inventor 柏连阳刘宏伟蒋慧丹尹华群刘学端梁伊丽秦崇
Owner HUNAN AGRI BIOTECH RES CENT
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