35 results about "Tyrosine ammonia-lyase" patented technology

A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL / TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.

A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL / TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.

The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.

The invention discloses a method for producing eriodictyol by reforming escherichia coli in metabolic engineering, belonging to the field of metabolic engineering. The step of transforming naringenin into the eriodictyol is successfully realized through a gene engineering technology by carrying out fusion expression on two genes, namely a flavone 3' hydroxylase gene tF3'H with a membrane binding sequence cut and a P450 reductase gene tCPR, in escherichia coli. A tyrosine ammonia lyase gene TAL from R.glutinis, 4-coumarate:coenzyme A ligase gene 4CL from P.crispum, a chalcone synthase gene CHS from P.hybrida and a chalcone isomerase gene CHI from M.sativa are simultaneously expressed to realize that naringenin is directly produced through tyrosine, so that an engineered strain for directly producing eriodictyol through tyrosine is established. According to the method, in order to increase the output of eriodictyol, an acetokinase ackA gene contained in an escherichia coli genome is removed by being knocked, an acetyl coenzyme A synthetase gene acs and an acetyl coenzyme A carboxylase gene ACC are excessively expressed, and the output of eriodictyol can finally reach 106.7 mg / L.

The invention discloses a biological preparation method of caffeic acid, which belongs to the technical field of microorganisms. The present invention selects high osmotic pressure Candida glycerol producing yeast CCTCC M93018 to construct engineering bacteria, knocks out L-tryptophan synthesis gene trp1 and L-phenylalanine synthesis gene pheA, and overexpresses 3-deoxy-D-arabinan Keto-7-phosphate synthase gene aro4 and bifunctional choroidate synthase and flavin reductase gene aro7, and tyrosine ammonia lyase, and 4-hydroxyphenylacetic acid-3-monooxygenase Mutation, in the yeast to achieve de novo synthesis of caffeic acid with glucose as carbon source, caffeic acid production reached 87.6mg / L. The preparation method of the invention has the characteristics of convenient operation, high conversion efficiency, low production cost, wide industrial application prospect and the like.

The invention discloses an engineering bacterium and its application for producing caffeic acid, and belongs to the technical field of biological engineering. The invention provides a recombinant bacteria capable of producing caffeic acid at low cost; the recombinant bacteria simultaneously expresses four enzymes, namely tyrosine phenol lyase, tyrosine ammonia lyase, L-lactate dehydrogenase, and NADH oxidase; Further, the recombinant bacteria of the present invention also knocks out the phenolic substance decomposition gene, and strengthens the expression of any one or more of the lactic acid transport gene, the catechol transport gene, and the coenzyme synthesis-related gene. The invention realizes the efficient production of caffeic acid, and has the advantages of simple process and few impurities, and has important industrial application value.

The invention discloses a biological method for producing resveratrol, which comprises the following steps: ligating target genes obtained by restriction enzyme digestion to an expression vector, wherein the target genes include the sequences of phenylalanine hydroxylase (PAH), tyrosine ammonia lyase (TAL) 4-coumarate:coenzyme A ligase (4CL) and resveratrol synthase (RS); transforming the constructed expression vector into a strain to obtain a recombinant engineered strain; and fermenting the recombined engineered strain. Compared with the prior art, the technical scheme provided by the invention uses the genetically engineered strain for fermentation to produce resveratrol, so as to realize denovo synthesis of resveratrol with no need of adding substrates. The method disclosed by the invention solves the source problem of resveratrol, and at the same time, reduces the production cost in a maximum extent, and is beneficial for industrial production.

The present invention relates to the field of genetic engineering, in particular to a rosavir analog, a genetically engineered bacterium producing the rosavir analog, and an application thereof. The rosevin analog has a structure shown in formula I, formula II, formula III or formula IV, and the genetically engineered bacterium contains phenylalanine ammonia lyase or tyrosine ammonia lyase, 4-coumaric acid coenzyme A Genes for ligase 4CL, cinnamoyl-CoA reductase CCR, and UDP glucosyltransferase UGT. The genetically engineered bacteria can ferment and produce Triandrin and Triandrin B (Formula IV); or Triandrin, Triandrin B, Triandrin C (Formula II) and Triandrin D (Formula III), and the yield of Triandrin and Triandrin B can reach up to 1.7g / L and 1.6g / L; or Luosaiwei B (formula I), the highest output can reach 1.6g / L.