Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of resveratrol and its production method, which is applied in the field of biomedicine, can solve the problems of complex steps, low production capacity, and low yield, and achieve the effects of being beneficial to industrial production, solving source problems, and reducing production costs
Inactive Publication Date: 2012-07-25
TIANJIN UNIV
View PDF3 Cites 17 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, it still cannot solve the fundamental problems faced by the plant extraction method, such as limited plant raw materials and unstable quality, low production capacity, planting is limited by seasons and geographical locations, etc.
Although the chemical total synthesis of resveratrol has been reported, the steps are complicated, the yield is low, and the environmental pollution is serious
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0023] The technical solutions of the present invention will be further described below in conjunction with specific embodiments. Using JCAT online gene design software, based on published gene sequences, conservatively design and optimize phenylalanine hydroxylase gene (PAH), tyrosine ammonia lyase gene (TAL), 4-coumaroyl-CoA ligase gene (4CL) and resveratrol synthase (RS) gene sequence, and these target genes were fully synthesized, wherein FASTpfu enzyme was purchased from Beijing Quanshijin Biotechnology Co., Ltd., and restriction enzymes were purchased from Fushitai Si Biotechnology (Shenzhen) Co., Ltd.
[0024] Sequence Optimization and Acquisition of Phenylalanine Hydroxylase Gene (PAH)
[0025] Preferably the phenylalanine hydroxylase gene sequence of Pseudomonas stutzeri was codon-optimized using jcat (http: / / www.jcat.de) for use in Escherichia coli BW25113 and Corynebacterium glutamicum For the expression of ATCC21673, the length of the optimized gene is 786bp, the...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a biological method for producing resveratrol, which comprises the following steps: ligating target genes obtained by restriction enzyme digestion to an expression vector, wherein the target genes include the sequences of phenylalanine hydroxylase (PAH), tyrosine ammonia lyase (TAL) 4-coumarate:coenzyme A ligase (4CL) and resveratrol synthase (RS); transforming the constructed expression vector into a strain to obtain a recombinant engineered strain; and fermenting the recombined engineered strain. Compared with the prior art, the technical scheme provided by the invention uses the genetically engineered strain for fermentation to produce resveratrol, so as to realize denovo synthesis of resveratrol with no need of adding substrates. The method disclosed by the invention solves the source problem of resveratrol, and at the same time, reduces the production cost in a maximum extent, and is beneficial for industrial production.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, and more specifically, relates to a method for producing resveratrol. Background technique [0002] Resveratrol (resveratrol, res), its chemical name is 3,4', 5-trihydroxy-trans-stilbene, also known as stilbene triphenol, and its molecular formula is C 14 h 12 o 3 , the molecular weight is 228.2. The pure product is a colorless needle-like crystal, insoluble in water (0.03g / L at 25°C), easily soluble in organic solvents such as ethanol, acetone, and ethyl acetate. With the deepening of research on resveratrol, it was found that resveratrol has a wide range of physiological and pharmacological activities, including anticancer activity, antioxidation, cardiovascular protection, free radical scavenging, anti-inflammatory, antibacterial, Immunomodulatory effects, estrogen-like activity, etc. Therefore, resveratrol has broad market prospects in the fields of health care products, cosmetics, a...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more