10 results about "Coenzyme A Ligases" patented technology

The invention discloses recombinant saccharomyces cerevisiae, a 11-seed oil fermentation product as well as a preparation method and application of the 11-seed oil fermentation product, and belongs to the technical field of synthetic biology and fermentation product preparation. According to the recombinant saccharomyces cerevisiae disclosed by the invention, a caffeic acid coenzyme A ligase 4CL coding gene, a chalcone isomerase CHI coding gene, a flavanone-3-hydroxylase F3H coding gene and a chalcone synthase CHS mutant coding gene are over-expressed, and the chalcone synthase CHS mutant is preferably Y69H / H71Y / Q161K. The preparation method of the 11-seed oil fermentation product comprises the following steps: fermenting a recombinant saccharomyces cerevisiae fermentation solution in the presence of a sophora flower bud extract and caffeic acid to obtain a first fermentation solution; fermenting the lactic acid bacteria fermentation liquor in the presence of 11 seed oil to obtain second fermentation liquor; and mixing the first fermentation liquid and the second fermentation liquid, continuing fermentation, and respectively collecting an oil phase and a water phase after fermentation is finished, so as to obtain a fermentation product. The product provided by the invention has multivitamin antioxidant activity.

The invention belongs to the technical field of biology, and discloses a saccharomyces cerevisiae engineering strain for producing naringenin, a construction method and application, the saccharomyces cerevisiae engineering strain contains tyrosine ammonia lyase gene TAL, 4-cumaric acid: coenzyme A ligase gene 4CL, chalcone synthase gene CHS, chalcone isomerase gene CHI, oligopeptide gene RIADn-RGGm and oligopeptide gene RIDD; the ARO10 gene, the FDC1 gene, the PAD1 gene and the PDC5 gene are knocked out. The engineering strain disclosed by the invention can be used for synthesizing naringenin by taking L-tyrosine as a substrate, and the engineering strain is stable in hereditary character and excellent in naringenin yield which can reach 27.45 mg / L; and the fermentation production of the product can be carried out through relatively simple method conditions, so that the method has a very wide application prospect.

A method for producing γ-Glu-Val-Gly is described, wherein the method includes the steps of cultivating a γ-Glu-Val-Gly-producing bacterium belonging to the family Enterobacteriaceae in a culture medium so that the γ-Glu-Val-Gly accumulates in the culture medium or the cells of the bacterium, or both, and collecting the γ-Glu-Val-Gly from the culture medium or the cells of the bacterium, or both. The bacterium has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity.

The present invention relates to a microorganism containing at least one heterologous gene encoding L-arginine:glycine amidinotransferase (AGAT), at least one heterologous L-threonine 3-dehydrogenase gene (tdh), and at least one heterologous glycine C-acetyltransferase (2-amino-3-oxobutanoate coenzyme A ligase) gene (kbl), as well as a method for the fermentative production of guanidinoacetic acid (GAA) using such a microorganism. The present invention also relates to a method for the fermentative production of creatine.

The present application relates to the field of genetic engineering and bioengineering, and particularly relates to a genetically engineered surfactin-producing bacterium and application thereof.The genome of the genetically engineered bacterium comprises the following: an expression frame of T7 RNA polymerase; an expression frame of a surfactin synthesis gene cluster srfAABCD; and an expression frame of a long-chain fatty acid-coenzyme A ligase gene lcfA; the expression frame of the surfactin synthesis gene cluster srfAABCD and the expression frame of the long-chain fatty acid-coenzyme A ligase gene lcfA further comprise a T7 promoter.The present application realizes efficient synthesis of surfactin by Bacillus subtilis, and the final shake flask yield reaches more than 19.3 g / L, which has great industrial application potential.

The application discloses a kind of p-coumaric acid or p-coumaric acid derivative production level improved microorganism and its construction method and application, comprising: the genetic target point related to p-coumaric acid metabolic pathway of microorganism is reformed, target point is 4-coumaroyl coenzyme A ligase, hydroxycinnamoyl coenzyme A hydratase lyase, hydroxyacyl coenzyme A dehydrogenase, beta-ketoacyl-coa thiolase, 3- (aryl) propenoate reductase one or more than two combinations, reformation is removed the partial activity or all activity of p-coumaric acid metabolic pathway related genetic target point.The application reduces the degree of degradation of p-coumaric acid by microorganism by reformation p-coumaric acid metabolic pathway related genetic target point, to improve the yield and / or production efficiency of p-coumaric acid and p-coumaric acid derivative produced by microorganism.

The invention relates to the field of gene engineering, in particular to saccharomyces cerevisiae gene engineering bacteria for producing verbascoside and echinacoside through de novo synthesis and application of the saccharomyces cerevisiae gene engineering bacteria. The genetically engineered bacterium contains a coded chorismate synthase mutant, a chorismate mutase mutant, shikimic acid kinase, cytochrome b5, glucose-6-phosphate-dehydrogenase, aromatic acetaldehyde synthase, UDP (User Datagram Protocol) glucosyltransferase, tyrosine ammonia lyase, phenylalanine ammonia lyase, cinnamate hydroxylase, 4-coumaric acid: coenzyme A ligase and acyltransferase. The invention relates to a gene encoding a UDP glucosyltransferase mutant, and more preferably, the gene encoding the UDP glucosyltransferase mutant comprises genes of a UDP glucosyltransferase mutant, a UDP glucosyltransferase mutant, a UDP glucosyltransferase mutant and a UDP glucosyltransferase mutant. Under a shake flask condition, verbascoside produced by the strain reaches 231 mg / L, echinacoside produced by the strain reaches 184 mg / L, and the strain has industrial application potential.

The invention relates to the technical field of genetic engineering and bioengineering, in particular to a surfactin-producing genetic engineering bacterium and application thereof. A genome of the genetically engineered bacterium comprises the following components; an expression cassette encoding a T7 RNA polymerase; an expression cassette of the surfactin synthesis gene cluster srfAABCD; and an expression cassette of the long-chain fatty acid-coenzyme A ligase gene lcfA; the expression cassette of the surfactin synthetic gene cluster srfAABCD and the expression cassette of the long-chain fatty acid-coenzyme A ligase gene lcfA also comprise a T7 promoter. According to the method, the surfactin is efficiently synthesized by the bacillus subtilis, the final shake flask yield reaches 19.3 g / L or above, and the method has huge industrial application potential.

The application discloses a kind of resveratrol engineering bacteria synthesized by p-coumaric acid, construction and application, belong to biotechnology field.The application is by expressing the coding gene of 4-coumaroyl coenzyme A ligase mutant and / or the coding gene of resveratrol synthase mutant and the coding gene of acetyl-coa carboxylase and / or the coding gene of acetyl-coa synthase, on the basis of significantly improving the catalytic activity of 4-coumaroyl coenzyme A ligase, resveratrol synthase respectively, by the enhanced expression of acetyl-coa, malonyl-coa, the obtained Yarrowia lipolytica engineering bacteria can efficiently utilize p-coumaric acid to produce resveratrol, the yield reaches 2.4g / L in flask fermentation, substrate conversion rate reaches 96.3% in 5L fermenter batch feeding fermentation, and the yield of resveratrol reaches 47.8g / L.

The invention discloses a saccharomyces cerevisiae engineering strain for reducing accumulation of naringenin and phloretin and efficiently producing phloretin and application of the saccharomyces cerevisiae engineering strain, and belongs to the technical field of microbial genetic engineering. According to the invention, the naringenin chalcone reductase gene MdNCR from apples is expressed in saccharomyces cerevisiae for the first time, and a new way for directly synthesizing phloretin from naringenin chalcone is constructed, so that the phloretin yield of the strain is increased by over 300% compared with that of Y22, the naringenin accumulation is reduced by more than 85%, and the problem of carbon metabolic flux shunting is effectively solved; 4-coumaroyl coenzyme A ligase and chalcone synthase are synergistically optimized aiming at a large amount of accumulation of the phloreic acid after introduction of an exogenous PAL pathway, and a'phloreic acid recovery cycle 'is constructed to re-introduce the phloreic acid into a synthesis main pathway; finally, the phloretin content of the engineering strain Y2226 is reduced to 22.3 mg / L, the phloretin yield is increased to 110.7 mg / L, and a solution is provided for accumulation of phloretin by-products synthesized by microorganisms.