43 results about "P-Coumaric acid" patented technology

The invention discloses a method for producing p-coumaric acid through double-enzyme cascade catalysis, which comprises the following steps: taking phenol, pyruvic acid and ammonium chloride as substrates, and carrying out cascade enzyme catalytic reaction on a tyrosine phenol lyase product and a tyrosine ammonia lyase product to synthesize p-coumaric acid. According to the method, phenol, pyruvic acid and ammonium chloride are used as substrates, a tyrosine phenol lyase product and a tyrosine ammonia lyase product are used as catalysts, the tyrosine phenol lyase product can convert the substrates into L-tyrosine, and the tyrosine ammonia lyase product can directly use the L-tyrosine as the substrate to convert the L-tyrosine into p-coumaric acid. Therefore, the tyrosine phenol lyase product and the tyrosine ammonia lyase product can realize cascade enzyme catalytic reaction in the same reactor, phenol with lower cost can be adopted as a conversion substrate, separation and purification of an intermediate product L-tyrosine are avoided, and the whole process flow is simple and efficient.

The application discloses a kind of compound Xianghua granules fingerprint's construction method, comprising the following steps: preparation of reference solution: p-coumaric acid, ferulic acid standard in methanol is dissolved;Preparation of test solution: different batches of compound Xianghua granules are dissolved in ultrapure water respectively, and post-processing is carried out;Respectively inject high performance liquid chromatography and detect, obtain the fingerprint of reference solution and the fingerprint of each batch sample;Using traditional Chinese medicine fingerprint similarity evaluation system analyzes the fingerprint of each batch sample, generates the control fingerprint of compound Xianghua granules.The method provided by the application can better reflect the multiple index component information of compound Xianghua granules.The fingerprint characteristic peak baseline of the application is smooth, and the sensitivity and separation degree are better, while the operation is quick and reliable, the cost is lower, can effectively, stably and quickly comprehensively monitor product quality, ensure the stability and quality controllability of product.

The invention discloses a Rogowski eutrophy alkali-producing engineering strain for producing p-coumaric acid as well as a construction method and application of the Rogowski eutrophy alkali-producing engineering strain. The preparation method comprises the following steps: by taking a chemoautotrophic microorganism Ropriavidus alcaligenes H16 as an original strain, firstly, by knocking out a synthetic route of a strain poly-3-hydroxybutyrate (PHB), introducing a tyrosine ammonia lyase gene from Rhodotorula glutinis to construct a basic engineering strain capable of producing p-coumaric acid, and then, carrying out fermentation on the basic engineering strain to obtain the p-coumaric acid. Then, endogenous 3-deoxy-7-phosphoheptanone acid synthase gene aroG1 and chorismate synthase gene aroC are overexpressed, and the total copy is increased, so that the p-coumaric acid synthetic pathway flux of the engineering strain is enhanced; and introducing NAD (P) transhydrogenase gene pntAB from Escherichia coli to improve the supply of cofactors, so as to finally obtain the eutrophy alcaligenes Rosei engineering strain capable of synthesizing p-coumaric acid by using CO2.

This invention relates to the fields of synthetic biology and biochemical engineering, and provides an engineered *Yersinia lipolytica* strain that enhances coumaric acid accumulation, its construction method, and its applications. The engineered *Yersinia lipolytica* strain of this invention uses *Yersinia lipolytica* as the substrate strain, expresses the tyrosine ammonia-lyase encoding gene RgTAL derived from *Rhodotorula glutinis*, and completely knocks out the aminotransferase encoding gene containing the nucleotide sequence shown in SEQ ID NO 1 (database number YALI0A08734g). RgTAL converts endogenous L-tyrosine to coumaric acid. This invention reduces dependence on complex metabolic regulation and increases the coumaric acid accumulation level in *Yersinia lipolytica*.

A MATZHU is prepared by using leaves of Gramineae (Graminae) and Bambusoideae plant as raw materials, and has a stable emerald color, and has an average powder particle size of 800 to 10,000 meshes, and has a total amount of dietary fiber of ≥60%, a content of lignin of ≥20% and a content of minerals of ≥7%, and comprises at least three or more bamboo leaf characteristic components, such as orientin, isoorientin, vitexin, isovitexin, adenosine, δ-hydroxylysine and p-coumaric acid. The method for the MATZHU preparation comprises, in turn, performing blanching and color protection, drying and superfine grinding the raw materials. By utilizing the thermal stability and the light stability of the MATZHU, the MATZHU may be used as a raw food material, a functional ingredient, or a dietary supplement.

According to the genetically engineered bacterium for high yield of p-coumaric acid and the application of the genetically engineered bacterium, tyrosine ammonia lyase from different sources and expression vectors with different copy numbers are combined to optimize metabolic pathways, so that the yield of p-coumaric acid is increased. The method specifically comprises the following steps: constructing recombinant expression vectors by using tyrosine ammonia lyase FjTAL from flavobacterium johnsonii and tyrosine ammonia lyase FsTAL from flavobacterium cissii and expression vectors pACYCDuet-1, pCDFDuet-1, pETCDuet-1 and pRSFDuet-1 with different copy numbers respectively by using a homologous recombination technology, transferring the recombinant expression vectors into escherichia coli BL21 (DE3) to obtain engineering strains pCA1-pCA8, and carrying out fermentation on the engineering strains pCA1-pCA8 to obtain the tyrosine ammonia lyase FjTAL from flavobacterium johnsonii and the tyrosine ammonia lyase FsTAL from flavobacterium cissii. Glucose is used as a substrate for fermentation and screening to obtain the high-yield engineering bacterium pCA8, and the yield of p-coumaric acid of the engineering bacterium pCA8 reaches 251.61 mg / L. By optimizing key enzyme expression, tyrosine is efficiently promoted to be converted into p-coumaric acid, and the method has the advantages of being low in cost, high in efficiency, environmentally friendly and the like, is suitable for industrial production and can be widely applied to the fields of biomedicine, food, cosmetics and the like.

The present application belongs to the field of livestock and poultry breeding and feed processing technology, and particularly relates to a feed additive, a nutritional supplement, a feed and application thereof. The present application provides application of p-coumaric acid in improving carcass and meat quality, improving feed conversion rate, reducing fat deposition of livestock and poultry and improving fat metabolism of livestock and poultry. The results of the examples show that p-coumaric acid can effectively improve the feed conversion efficiency of growing pigs in the growth stage, which provides a reference for reducing feed waste in production and realizing cost reduction and benefit increase of animal husbandry; under the premise of not reducing or even improving the growth performance and slaughter rate of growing and fattening pigs, the p-coumaric acid can reduce the fat thickness, improve the pig carcass rating, improve the meat quality, and is beneficial to realize efficient production of high-quality pork and improve the market competitiveness of pork in China; the p-coumaric acid can also reduce the blood lipids of chickens, reduce fatty liver and reduce subcutaneous fat deposition, maintain normal metabolism of chickens and improve the health of chickens.

The invention provides a herbicide for preventing and treating cyperus rotundus in cooperation with glyphosate. The herbicide is prepared from bacillus velezensis fermentation liquor, phenolic acid and glyphosate. The phenolic acid comprises any one or a combination of more of p-coumaric acid, cinnamic acid, p-hydroxybenzoic acid and caffeic acid. The bacillus velezensis fermentation liquor and phenolic acid components are introduced and form a synergistic effect with the glyphosate, so that the dosage of a single dosage of the glyphosate is reduced, the control effect is guaranteed, meanwhile, the potential pressure of chemical pesticide input on the ecological environment is reduced, and the environmental compatibility of a weeding system is improved.

According to the tyrosine ammonialyase mutant and the application thereof, 28 amino acids at the N end of tyrosine ammonialyase are deleted or replaced with 25 or 12 amino acids to obtain the mutant, the enzyme activity is effectively improved by 13.96-27.83% compared with that of a wild type, meanwhile, the coumaric acid biosynthesis efficiency is improved, by-product accumulation is reduced, and the yield of the tyrosine ammonialyase mutant is improved. And industrial application of p-coumaric acid biosynthesis is promoted.

This invention discloses a method for preparing and applying a coumaric acid-modified MOF nanopesticide loaded with chlorantraniliprole, belonging to the field of pesticide formulation technology. The nanopesticide comprises: an iron-based metal-organic framework carrier MIL-101(Fe); chlorantraniliprole loaded within the pores of the carrier; and coumaric acid modified on the surface of the carrier through electrostatic interactions. The preparation method includes three steps: MIL-101(Fe) carrier synthesis, chlorantraniliprole loading, and coumaric acid surface modification. The process is simple and the conditions are mild. This invention utilizes the alkaline environment of the fall armyworm's gut to trigger carrier degradation, achieving targeted and controlled release of active molecules. Simultaneously, the released MIL-101(Fe) is reduced under the action of GSH in the gut, generating reactive oxygen species through the Fenton reaction, forming a synergistic insecticidal effect with coumaric acid. Experiments have shown that this nanopesticide exhibits far superior insecticidal activity against fall armyworm compared to single agents or carriers, possessing excellent insecticidal activity and environmental compatibility, making it suitable for green agricultural production.

The application discloses an engineered strain for synthesizing resveratrol by using p-coumaric acid as a substrate, construction and application thereof, and belongs to the technical field of biotechnology. The active sites of 4-coumaroyl-CoA ligase and resveratrol synthase are semi-rationally designed and modified, the optimal mutants 4CL1 (M4) and VST1 (N3) are expressed in a Yarrowia lipolytica strain, and the obtained engineered strain can efficiently convert p-coumaric acid into resveratrol. The yield of resveratrol in a shake flask fermentation reaches 724.6 mg / L. Then, the two mutants are fused and expressed by using a rigid linker, and the Yarrowia lipolytica engineered strain for producing resveratrol by using p-coumaric acid as a substrate is obtained. In a 5-L fermenter, batch feeding fermentation is carried out, the substrate conversion rate reaches 92.3%, and the yield of resveratrol reaches 35.8 g / L.

This invention discloses a strain that degrades various lignin-derived aromatic compounds and its application in detoxification of hydrolysate. The strain is classified as *Rhodococcus ethereans*. Rhodococcus ether-eating Strain N1, deposited at the China Center for Type Culture Collection (CCTCC) on August 11, 2022, with accession number CCTCC NO: M 20221270, can degrade p-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillin, coniferyl alcohol, and syringaldehyde. After lignin depolymerization, derivative phenolic inhibitors are formed, mainly divided into three types: S-type (syringaldehyde), G-type (ferulic acid), and H-type (p-coumaric acid), which affect the fermentation of the strain. The Rhodococcus bacterium in this invention can efficiently degrade SGH-type monomers, achieving a removal efficiency of up to 68.4% when applied to the biological detoxification of hydrolysate inhibitors. Strain N1 has significant application value in improving the conversion efficiency of lignocellulose hydrolysate and reducing the detoxification cost in industrial production.

The application discloses a hypoglycemic and hypolipidemic composition from the leaves of pandamus, a preparation method and application thereof. The composition contains p-coumaric acid, rutin, kaempferol-3-O-rutinoside, pandamarilactonine-A, pandamarilactone-1 and pandamarilactone-2. The composition with a preferred mass ratio has a certain synergistic effect. Under the premise of a consistent total dose, the composition is superior to p-coumaric acid, rutin and kaempferol-3-O-rutinoside in improving insulin resistance and reducing fat deposition, and is also superior to a pandamus alkaloid composition composed of pandamarilactonine-A, pandamarilactone-1 and pandamarilactone-2, which indicates that there is a synergistic effect among the components of the composition.

A nanomaterial is gold or silver nanoparticle having a nanoparticle surface that consists of one or more of the following extracts from sweet gum leaves: shikimic acid, sweet gum leaf family of Catechins, sweet gum leaf family of Quercetin, Sweroside, Afzelechin, p-Coumaric Acid, Epiafzelechin Trimethyl Ether, Myricetin, Naringenin, Phloretin, Procyanidin B1 and related polyphenols, flavonoids and alkaloids. An antiviral / antibacterial agent includes sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral / antibacterial exceeding a 3 log 10 reduction in viral titer.

The application discloses a Yarrowia lipolytica strain for synthesizing (2S)-naringenin and application thereof, and belongs to the field of genetic engineering and bioengineering technology. The application improves the yield of (2S)-naringenin by 60% through semi-rational modification of a key enzyme CHS, and further integrates the modified CHS into the yeast genome. In addition, the application promotes the conversion of coumaric acid to (2S)-naringenin by strengthening the shikimic acid pathway and enhancing the supply of auxiliary factors, so that the yield of (2S)-naringenin reaches 776 mg / L after 96 hours of fermentation of the constructed strain in a 24-hole plate, and the yield reaches 8.65 g / L in a 5L fermenter. The application lays a foundation for subsequent biosynthesis of flavonoids, and has potential value and significance for the development of synthetic biology.

The invention provides a composition comprising oleanolic acid and the salts, prodrug and solvates thereof; and one or more compounds selected from the following group: ellagic acid, adenine, betaine, choline, miquelianin, p-coumaric acid, pipecolic acid, protocatechuic acid and tyramine, and the salts, prodrugs and solvates thereof. Such a composition is for use in reducing lipid levels in sebocytes, and the composition may find use in treating acne vulgaris and rosacea.

The application discloses a method for constructing a compound health-care wine fingerprint spectrum, which comprises the following steps: preparing a test sample solution, preparing a control sample solution, performing multi-index quantitative determination, determining the fingerprint spectrum, and performing fingerprint spectrum analysis and processing. The method can simultaneously detect 21 characteristic fingerprint peaks in the compound health-care wine, and 4 fingerprint peaks are identified by comparison with the control sample, which are chlorogenic acid, bitter crocins, p-coumaric acid, ferulic acid and the like; and more fingerprint spectrum characteristic peaks can be obtained in the shortest possible separation time.

The invention discloses a method for rapidly determining various phenolic acid substances in a fermentation product, and belongs to the technical field of detection. The determination method comprises the following steps: 1. Taking a to-be-detected substance, and extracting phenolic acid substances in the to-be-detected substance through an alcohol precipitation-SAX solid-phase extraction combined technology to obtain a to-be-detected sample; analyzing the to-be-detected sample by adopting ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry to obtain the content of the target substance in the to-be-detected substance; the target substance is a non-volatile phenolic acid substance. Accurate quantification of trace and trace caffeic acid, dihydrocaffeic acid, ferulic acid, chlorogenic acid, gallic acid, vanillic acid, syringic acid, p-coumaric acid, salicylic acid and (+ / -)-catechin in fermentation products within 8-15 min is achieved by combining impurity removal and concentration pretreatment with ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry. According to the method, the pretreatment mode is convenient to operate, the detection process is fast, and the detection result is accurate.

The present application relates to the technical field of bioengineering, and in particular to a kind of immobilized tyrosine ammonia lyase genetically engineered bacteria microbial cell and its preparation and application.The wet bacteria body is mixed with Gly-NaOH buffer solution first, to obtain bacterial suspension;The wet bacteria body is the wet bacteria body of tyrosine ammonia lyase containing engineering bacteria;Then hydroxylated modified diatomite is mixed with bacterial suspension, to obtain mixture;Finally, PEI aqueous solution is added in the mixture for once crosslinking treatment, then glutaraldehyde is added for twice crosslinking treatment, and the immobilized tyrosine ammonia lyase genetically engineered bacteria microbial cell is obtained after treatment.The immobilized tyrosine ammonia lyase genetically engineered bacteria microbial cell is recycled in the present application, and the substrate conversion rate is more than 70% when repeated reaction is 20 batches, and the catalyst still retains more than 70% of initial enzyme activity.The immobilized catalyst has the industrial application potential of biocatalytic production of p-coumaric acid.

The invention provides a recombinant saccharomyces cerevisiae strain for improving the yield of aromatic acid as well as a construction method and application of the recombinant saccharomyces cerevisiae strain. A saccharomyces cerevisiae strain PC01 for synthesizing protocatechuic acid and p-coumaric acid is used as a chassis, fbp1, pfk1, pfk2 and shb17 are knocked out, and an endogenous escape path and invalid cycle of saccharomyces cerevisiae are blocked. Meanwhile, a p-coumaric acid synthesis pathway is knocked out, so that the inhibition of p-coumaric acid on the growth of the recombinant saccharomyces cerevisiae is relieved. Then, overexpressing a protocatechuic acid synthetase gene so as to enhance the metabolic flux of the protocatechuic acid, and overexpressing Aro3D154N, Aro4K229L, EcAroB and EcAroD so as to enhance the synthesis of the main carbon flow to the protocatechuic acid. And finally, integrating other aromatic acid metabolic pathways to realize biosynthesis of salicylic acid, p-hydroxybenzoic acid and gallic acid. And the universality of the engineering strain is verified. The recombinant strain provided by the invention takes glucose as a carbon source, so that not only is the production cost reduced, but also the stable hereditary character can be maintained, and the high yield of aromatic acid is ensured.

This invention belongs to the field of genetic engineering, and specifically relates to a 4-coumaric acid-coenzyme A ligase gene Gl4CL from *Gynostemma pentaphyllum*, its encoded product, and its applications. This invention is the first to clone and prepare the 4-coumaric acid-coenzyme A ligase gene Gl4CL from the medicinal plant *Gynostemma pentaphyllum*. The nucleotide sequence of this gene is shown in SEQ ID NO.1, and the amino acid sequence of the product encoded by this nucleotide sequence is shown in SEQ ID NO.2. The 4-coumaric acid-coenzyme A ligase gene Gl4CL is a key regulatory gene in the flavonoid biosynthesis pathway of *Gynostemma pentaphyllum*. This enzyme can be used in the preparation of p-coumaryl-CoA using p-coumaric acid as a substrate, further promoting the biosynthesis of flavonoid compounds. This invention can provide an effective method to meet the huge market demand for flavonoid compounds.

The invention belongs to the technical field of food processing, and relates to a phenolic acid-hydrolyzed casein compound as well as a preparation method and application thereof. Specifically, casein is hydrolyzed by alkaline protease to obtain casein with the molecular weight of 1t; a high antioxidant active component of 3KDa; the casein hydrolysate is mixed with a phenolic acid solution to form a compound. An in-vitro simulated gastrointestinal digestion test shows that the polyphenol content and the total antioxidant capacity of the compound are obviously higher than those of a single phenolic acid group, and the digestion stability of phenolic acid is improved. Animal experiments prove that the compound intervention can shorten the defecation time and improve the gastrointestinal propulsion rate, and the digestion promoting effect is superior to that of a single component and is equivalent to that of a positive drug mosapride; wherein the caffeic acid and the p-coumaric acid-casein hydrolysate compound have the best effect, and the synergy index is CIlt; 1, a synergistic effect is proved;

This invention relates to the field of biotechnology, and discloses an engineered *Yarrowia lipolytica* strain that produces p-coumaric acid using glucose as a substrate, its construction method, and its applications. Construction method: A tyrosine ammonia-lyase gene is integrated using a CRISPR / Cas9 localization and integration method. TAL In *Yarrowia lipophila* strains, the DAHP synthase gene was enhanced. ARO4 , DHS1 and AROG Overexpression of tyrosine synthase TYR Genes and histidine phosphotransferases HIS5 The protease gene was further integrated to synthesize exogenous phenylalanine deamination and hydroxylation pathway genes for p-coumaric acid, including phenylalanine ammonia-lyase gene, cinnamate hydroxylase gene, and P450 reductase gene. The p-coumaric acid produced by the engineered *Yarrowia lipolytica* strain of this invention can reach a maximum yield of 1.7 g / L in shake flasks, and a yield of 30 g / L in a 5 L fed-batch fermentation tank, demonstrating significant industrial application value.

This invention discloses a coumaric acid biosensor and its application. The coumaric acid biosensor is based on the coding gene of the CarR mutant, P... lpp1.6 Promoter, gene encoding green fluorescent protein, and P that controls green fluorescent protein expression car The promoter mutant is located on the expression vector pTrc99a. The biosensor of this invention enables real-time and sensitive monitoring of coumaric acid concentration in recombinant *E. coli* strains containing a coumaric acid biosensor. It can be combined with high-throughput mutagenesis and screening systems, facilitating rapid and efficient screening of high-yielding coumaric acid engineered strains, and providing technical support for the efficient microbial fermentation production of coumaric acid and its derivatives. Constructing a coumaric acid biosensor based on transcriptional regulatory factors provides a new strategy for high-throughput screening.

The application discloses a kind of p-coumaric acid or p-coumaric acid derivative production level improved microorganism and its construction method and application, comprising: the genetic target point related to p-coumaric acid metabolic pathway of microorganism is reformed, target point is 4-coumaroyl coenzyme A ligase, hydroxycinnamoyl coenzyme A hydratase lyase, hydroxyacyl coenzyme A dehydrogenase, beta-ketoacyl-coa thiolase, 3- (aryl) propenoate reductase one or more than two combinations, reformation is removed the partial activity or all activity of p-coumaric acid metabolic pathway related genetic target point.The application reduces the degree of degradation of p-coumaric acid by microorganism by reformation p-coumaric acid metabolic pathway related genetic target point, to improve the yield and / or production efficiency of p-coumaric acid and p-coumaric acid derivative produced by microorganism.

The present application relates to papermaking wastewater high-value utilization technical field, specifically to a kind of method for preparing high value-added amino acid using lignin in papermaking wastewater, comprising: first using acid to adjust the pH of papermaking black liquor to 5-9, then join lanthanum manganese composite oxide catalyst and pass in O2 Oxidative depolymerization reaction obtains p-coumaric acid solution;P-coumaric acid solution is added to ruthenium iron catalyst, ammonia and carried out in H2 Atmosphere ammonia addition reaction to obtain L-tyrosine and beta-tyrosine.The two-step catalytic reaction method provided by the present application prepares amino acid from lignin in papermaking wastewater, not only reduces the pollution of papermaking black liquor, but also improves its application added value.

The invention relates to the technical field of biological medicines, and particularly discloses application of p-coumaric acid in preparation of a medicine for treating nephropathy, and the nephropathy is acute kidney injury or acute glomerulonephritis. According to the p-coumaric acid, the expression of SLC7A11 and / or GPX4 is up-regulated by inhibiting ferroptosis, so that the effect of relieving the acute kidney injury is achieved. In addition, p-coumaric acid can significantly improve the content of BUN and SCr in serum of an acute glomerulonephritis mouse model and the ROS level of the kidney. In conclusion, p-CA can become a novel potential candidate drug for treating acute kidney injury or acute glomerulonephritis.

The invention provides a recombinant engineering bacterium for synergistically co-producing flavone 7-O-glucoside and application of the recombinant engineering bacterium, the recombinant engineering bacterium takes saccharomyces cerevisiae SQ05 as an original strain, exogenous expression of a Pc4CL gene, an SjCHS1 gene and an MsCHI gene is realized, the gene expression quantity of a naringenin synthetic route is increased, supply of coumaric acid and cytoplasmic acetyl coenzyme is increased, and efficient synthesis of naringenin is realized. And then, exogenous MpOMT, AtGT, Cm1, 2-RhaT and OIRHM genes are inserted, so that the coordinated co-production of the flavone 7-O-glucoside is realized. Further, the synthesis proportion of isosakuranetin 7-O-glucoside and naringenin 7-O-glucoside is adjusted by increasing the expression quantity of methyltransferase and enhancing the supply of a methyl donor and a glycosyl donor. The total yield of the target product is improved through a one-bacterium productive mode, and the difficulty and cost of a downstream purification process are remarkably reduced.

The invention belongs to the technical field of livestock and poultry breeding and feed processing, and particularly relates to a feed additive, a nutritional supplement, feed and application thereof. The invention provides an application of p-coumaric acid in any one or more than two of improvement of carcass and meat quality, improvement of feed conversion rate, reduction of livestock and poultry fat deposition and improvement of livestock and poultry fat metabolism. Results of the embodiment show that p-coumaric acid can effectively improve the feed conversion efficiency in the growing stage of the fattening pigs, and reference is provided for reducing feed waste in production and achieving cost reduction and benefit improvement of animal husbandry; on the premise of not reducing or even improving the growth performance and the dressing percentage of growing-finishing pigs, the p-coumaric acid can reduce the fat thickness, improve the pig carcass rating and improve the meat quality at the same time, thereby being beneficial to realizing high-efficiency production of high-quality pork and improving the market competitiveness of the pork in China; the p-coumaric acid can also reduce the blood fat of the chicken, reduce the fatty liver and subcutaneous fat deposition, maintain the normal metabolism of the chicken and improve the health of the chicken.

The invention relates to the technical field of eggplant preparation, in particular to a preparation method of eggplants with high anthocyanin retention. The invention relates to a preparation method of eggplants with high anthocyanin retention. The preparation method comprises the following steps: preparing pre-treated eggplant slices; performing radio frequency blanching and radio frequency pulse vacuum drying; preparing a spiraea salicifolia extract and a clover extract; the eggplants with high anthocyanin content are prepared. After p-coumaric acid is dissolved to prepare a solution, eggplant slices are added, then vacuum treatment is utilized to effectively remove air in eggplant tissue gaps, and then the p-coumaric acid solution is forced to enter eggplant cell gaps through sudden pressure release, so that the eggplant slices are quickly and effectively removed. Eggplant cells can utilize p-coumaric acid to synthesize acylated anthocyanin with higher heat resistance, light stability and antioxidant activity, and then acylated eggplant slices are immersed in a color protection solution containing citric acid and calcium chloride, so that the retention rate of anthocyanin in eggplants can be effectively increased.