127 results about "P-Coumaric acid" patented technology

The invention relates to a method for simultaneously preparing trans-ferulic acid, p-coumaric acid and pentosan, which comprises the following steps: processing a cellulose material with a low-concentration alkali to release coumaric acid, and hydrolyzing with a high-concentration alkali to release ferulic acid and pentosan; ultrafiltering the alkaline hydrolytic solution, precipitating the concentrated solution with ethanol to obtain pentosan; and adsorbing coumaric acid and ferulic acid in the filtrate with cation exchange resin, eluting with an ethanol-acid-water mixed eluent, removing ethanol from the eluent, crystallizing, centralizing or filtering the eluent at a low temperature or a normal temperature to obtain ferulic acid or coumaric acid crystals, and recrystallizing to obtain the high-purity product. The alkaline hydrolytic solution can be recovered and used for hydrolyzing the material, thereby obviating the production of a great amount of wastewater due to the alkaline hydrolysis. The method can produce ferulic acid and coumaric acid widely used in the industries of food, medicine and cosmetics, and pentosan used for producing xylooligosaccharide as food gum by utilizing solid wastes produced in agricultural and food processing, thereby achieving significant economic and social meanings.

The invention discloses a method for simultaneously determining twenty-two flavones and phenolic acids in citrus fruits by adopting high performance liquid chromatography-diode array detector-fluorescence detector (HPLC-DAD-FLD). The method is capable of simultaneously determining twenty-two phenolic compounds in citrus fruits such as gallic acid, synephrine, chlorogenic acid, protocatechuic acid,caffeic acid, p-coumaric acid, rhamnosylvitexin, eriocitrin, ferulic acid, rutin, benzoic acid, narirutin, naringin, hesperidin, diosmin, neohesperidin, quercetin, naringenin, kaempferol, nobiletin,hesperetin, acacetin and the like, derivatization is not needed, and the method is high in accuracy, high in sensitivity and excellent in repeatability.

The invention discloses a method for preparing p-coumarate by catalyzing lignin depolymerization through hierarchical pore molecular sieve loaded molybdenum oxide. The method comprises the following steps: by taking lignin as a raw material, adding a reaction medium and a hierarchical pore molecular sieve loaded molybdenum oxide catalyst, replacing with nitrogen, pressurizing to 0.1-1MPa, heatingto 120-160 DEG C, reacting for 2-10 hours while stirring, removing the catalyst after the reaction, and catalytically degrading the lignin into p-coumarate. By regulating and controlling the catalyststructure, the reaction medium and the reaction condition factors, high-yield and high-selectivity depolymerization of lignin is achieved, p-coumarate serving as a high-added-value chemical is obtained, and the purpose of high-valued utilization of lignin is achieved. The heterogeneous catalyst is used, the process is simple, reaction conditions are mild, lignin can be selectively converted to obtain the target product p-coumarate, and the yield and selectivity of p-coumarate can reach up to 11.78 wt.% and 85.24 wt.% respectively.

The invention discloses a method for preparing p-coumalic acid by using spartina alterniflora. Spartina alterniflora extraction liquid is subjected to ethyl acetate extraction; then, water phases aretaken; next, n-butyl alcohol is used for extraction; n-butyl alcohol phase extraction liquid is concentrated to obtain n-butyl alcohol phase crude paste; the n-butyl alcohol phase crude paste is sequentially subjected to positive phase silica gel column chromatography, ODS reversed phase chromatography and gel column chromatography; finally, separation and purification are performed through a highperformance liquid phase chromatography technology, thus obtaining the p-coumalic acid. Pharmacological research results show that the p-coumalic acid has obvious uric acid reduction and blood sugarreduction effects on mice with hyperuricemia.

The invention discloses a novel p-coumaric acid sulfonate derivative, a preparation method and application thereof. The structural formula of the novel p-coumaric acid sulfonate derivative is shown asformula (I) in the specification, wherein R is selected from the following groups shown as the specification. The compound provided by the invention has potential pharmaceutical activity, and the preparation raw material p-coumaric acid can be obtained by natural extraction method, thus having a positive effect on resource utilization of spartina alterniflora.

The invention relates to a herbicide for preventing and removing semen cuscutae. The herbicide comprises an effective constituent agent A, an effective constituent agent B and an additive as the following matching ratios: the agent A is one of 20-100 parts by volume of efficient haloxyfop-methyl with the mass concentration of 95% and 2.5-12 parts by weight of quizalofop-p-ethyl; the agent B is one of 450-600 parts by weight of coumarin and 500-650 parts by weight of p-coumaric acid; the additive is 150-200 parts by weight of ethoxy modified trisiloxane with the mass concentration of 100%; the parts by weight are calculated by the active compound. The herbicide has a favorable prevention effect on semen cuscutae, has the fatality rate of up to 95%, is low in toxicity, and is safe to human bodies, livestock and water sources.

The invention relates to application of a chitosan derivative in bacterial wilt prevention and treatment. The structure of the chitosan derivative is shown in the formula (I), wherein R represents H or caffeic acid, or ferulic acid, or cinnamic acid, or p-coumaric acid or chlorogenic acid with a decarboxylated hydroxyl group. The medial lethal concentration of the chitosan derivative used for restraining bacterial wilt pathogenic bacteria is provided, the low-concentration chitosan derivative can be used for effectively restraining bacterial wilt bacteria, an effective method for preventing and treating bacterial wilt is provided, and the environment is not polluted.

The invention discloses a medicinal composition for treating a turtle cavity disease. The medicinal composition for treating the turtle cavity disease comprises the following Chinese herbs in parts by weight: 15-30 parts of hedyotis diffusa, 20-30 parts of groundsel, 5-15 parts of gallnut, 10-25 parts of golden cypress, 2-10 parts of wild chrysanthemum flower and 5-15 parts of onion. The invention further discloses a preparation of the medicinal composition. The hedyotis diffusa and the groundsel have a very strong anti-virus effect, so that the hedyotis diffusa and the groundsel as effective components can prevent and treat the turtle cavity disease better, with the cure rate as high as 99%. In the preparation method, the hedyotis diffusa is extracted with a supercritical carbon dioxide extraction method, so that effective components of the hedyotis diffusa, namely hentriacontane, stigmasterol, ursolic acid, oleanolic acid, beta-sitosterol, beta-sitosterol-D-glucoside and p-coumaric acid, have relatively high contents. The preparation method is simple and feasible and is applicable to large-scale production.

The invention discloses a method for enhancing photostability of folic acid and belongs to the technical field of medicine preparation. The method disclosed by the invention comprises the following steps: adding a hydroxyl cinnamic acid derivative in the mass concentration more than or equal to 1/00 of the mass concentration of the folic acid into the folic acid, mixing and then preparing into a folic acid-hydroxyl cinnamic acid derivative mixed solution. The photostability of the folic acid in the folic acid-hydroxyl cinnamic acid derivative mixed solution prepared according to the method disclosed by the invention is high; in the folic acid-p-coumaric acid or folic acid-caffeic acid mixed solution at a mass ratio of 1:1, the residual rate of the folic acid after UV irradiation for 240 minutes is still 100%; the light degradation of the folic acid can be completely restrained; and the product can be applied to drugs, foods and cosmetics.

A biosynthetic method of making pterostilbene including expressing a 4- coumaratexoenzyme A ligase (4CL) in a cellular system, expressing a stilbene synthase (STS) in the cellular system, expressing a resveratrol O-methyltransferase (ROMT) in the cellular system, feeding p-coumaric acid to the cellular system, growing the cellular system in a medium, and producing pterostilbene.

The invention discloses a method for preparing a hypolipidemic medicine ciprofibrate with p-coumaric acid. The method comprises the following specific steps: p-coumaric acid (I) is subjected to a decarboxylation reaction under the effect of an alkaline catalyst, such that p-hydroxystyrene (II) is obtained; p-hydroxystyrene (II) is subjected to a reaction with 2-haloisobutyrate under the effect of alkali, such that an etherified product (III) is obtained; under an alkaline condition, the etherified product (III) and chloroform are subjected to a cyclization reaction under the effect of a phase transfer catalyst, such that a cyclized product (IV) is obtained; the cyclized product (IV) is subjected to alcoholysis and acidification in an alkali solution; and recrystallization is carried out, such that ciprofibrate (V) is obtained. The method provided by the invention has the advantages of short synthesis process, safe operation and easy post-treatment. The method is suitable for large-scale industrialized productions, and almost has no possibility of causing accidents such as explosion. During the entire reaction process, only conventional acid, alkali and solvent are used, such that the cost is low. The solvent can be recovered and reused, such that the method is environment-friendly. With the method, the yield is improved by more than 20%.

The invention discloses a method for biosynthesizing a high-added-value compound by using lignocellulose derivatives, which comprises the following steps: A, modifying Escherichia coli to obtain a biocatalyst; B, synthesizing a high-added-value compound by taking a lignocellulose derivative as an initial raw material through a biocatalyst, wherein the lignocellulose derivative comprises at least one of p-coumaric acid and ferulic acid, and the high-added-value compound comprises at least one of gastrodin, arbutin, salidroside and derivatives thereof such as hydroquinone, tyrosol, hydroxytyrosol and styracitol. According to the method, escherichia coli is modified through a genetic engineering means, three new enzymatic reaction ways are constructed, and various high-added-value compounds including gastrodin, arbutin, salidroside and the like are efficiently synthesized by utilizing lignocellulose derived aromatic compounds p-coumaric acid and ferulic acid, wherein the product yield reaches gram-level or above.

A genetically engineered bacterium for synthesizing resveratrol is disclosed. The genetically engineered bacterium is obtained by knocking tyrR and trpED which are genes limiting tyrosine synthesis from glucose out of E.coli BW25113, and the chromosome is recombined with a tyrosine deaminase gene tal of rhodotorula glutinis, a petroselinum crispum p-coumaric acid: coenzyme A ligase gene 4cl and a stilbene synthase gene sts of grapes. The genetically engineered bacterium adopts glucose as a substrate to synthesize the resveratrol.

The invention relates to cytochrome P450 reductase as well as coding gene and application thereof. The invention discloses cytochrome P450 reductase 1 of lycoris aurea as an amaryllidaceae plant for the first time; the cytochrome P450 reductase 1 has favorable coenzyme specificity and can be used for assisting a cytochrome P450 enzyme in exerting catalytic activity to modify a synthetic product of a substrate of the cytochrome P450 enzyme in an oxidative manner. The invention also discloses a polynucleotide for coding the cytochrome P450 reductase, a carrier and a host cell for expressing the cytochrome P450 reductase and a method for producing p-coumaric acid.

The invention relates to p-coumaric acid-3-hydroxylase as well as a coding gene and an application thereof. The p-coumaric acid-3-hydroxylase of lycoris plant lycoris aurea is disclosed for the first time, has good substrate specificity and region selectivity, and can catalyze hydroxylation of 3-position C of p-coumaric acid to produce coffeic acid. The invention further discloses polunecleotide coding the p-coumaric acid-3-hydroxylase, a vector and a host cell expressing the p-coumaric acid-3-hydroxylase as well as a coffeic acid production method.

The invention relates to a food product comprising p-coumaric acid, wherein the level of p-coumaric acid is from 0.05 to 1.0 wt %, wherein said product is a fat based spread comprising from 10-85 wt % of fat and 10-90 wt % of water, or a drink, especially a dairy based drink, wherein the drink comprises from 10 to 95 wt % of a dairy base such as cow milk, soy milk or yoghurt, especially preferable cow milk or yoghurt. The food product has vaso-relaxating properties but still has an acceptable taste and color.

The invention discloses a method for extracting ferulic acid, p-coumaric acid and pentosan from corn husks, which belongs to the technical field of chemical industry. The method includes firstly, soaking corn husks into alkali solution, filtering and extracting p-coumaric acid after filtrate is neutralized to meet PH (potential of hydrogen) = 3-4 and concentrated, secondly, soaking the corn husks filtered from the first step into alkali solution, filtering and extracting ferulic acid by ethyl acetate after the filtrate is neutralized to meet PH (potential of hydrogen) = 3-4 and vacuum concentration is completed, thirdly, merging aqueous phase after the extraction of ethyl acetate in the step one and the step two, fourthly, desalinizing and condensing by means of nanofiltration, and fifthly, collecting pentosan by means of centrifugal settling after precipitated pentosan is dissolved out. The method for extracting ferulic acid, p-coumaric acid and pentosan from corn husks is short in production cycle and lower in solvent consumption, and thereby production efficiency can be further improved for enterprises.

The invention discloses pharmaceutical composition for post-operative nursing for cholangitis and a preparation method of the pharmaceutical composition. The pharmaceutical composition for post-operative nursing for the cholangitis comprises components in parts by weight as follows: 12-30 parts of p-coumaric acid, 15-32 parts of naringin, 10-28 parts of cordacin, 10-25 parts of a water shield extract, 15-26 parts of mangiferin, 8-18 parts of red halloysite nano-powder, 12-24 parts of limonene, 16-28 parts of eucalyptol, 14-20 parts of chlorogenic acid, 6-12 parts of levamisole hydrochloride, 40-80 parts of a magnesium sulfate solution, 50-100 parts of corn steep liquor, 8-16 parts of potassium sorbate and 13-25 parts of mixed amino acid. Effective components of Chinese herbal medicines are added on the basis of Western medicines, the pharmaceutical composition has effects of resisting bacteria, diminishing inflammations, inhibiting intestinal infection and preventing cholestasis, effectively regulates functions of the liver, the gall bladder and intestines of a patient and improves various anomaly indexes of the body, so that relapse of the cholangitis is prevented, and post-operative body recovery of the patient can be accelerated. The pharmaceutical composition is simple in preparation process and convenient to use and has the great clinical significance.

The invention discloses a method for improving the efficiency of ferulic acid and p-coumaric acid separation. According to the method, basic hydrolysis and acid hydrolysis are adopted in sequence to separate ferulic acid and p-coumaric acid from bagasse cell walls, and GC-MS quantitative analysis is carried out on the content of ferulic acid and p-coumaric acid. According to the method, a comprehensive method including basic hydrolysis and acid hydrolysis in sequence is adopted to separate ferulic acid and p-coumaric acid from bagasse cell walls, thereby eliminating interference of hemicellulose and lignin; ethyl acetate is taken as an extraction solvent, thereby obviously improving the yield of ferulic acid and p-coumaric acid; and GC-MS fast efficient quantitative analysis is carried outon ferulic acid and p-coumaric acid. The method establishes the foundation for efficient separation and industrial application of ferulic acid and p-coumaric acid.

The invention discloses a nano drug delivery system based on a p-coumaric acid polymer and a preparation method and an application thereof. The method synthesizes a biocompatible and biodegradable polymer PCA by a solution polycondensation method by using natural phenolic acid and p-coumaric acid as monomers; and based on the polymer PCA, the PCA nano drug delivery system is prepared by a nanoprecipitation method. The system is uniform in size and stable in nature, and can effectively load hydrophobic anticancer drugs and improve their bioavailability, significantly enhances the anti-tumor treatment effect in vitro and in vivo, and reduces the toxic side effects of the drug. In addition, a polymer carrier itself also exhibits certain anti-tumor activity, which can help enhance the efficacyof anti-cancer drugs, and opens up a new way for the application of p-coumaric acid in anti-tumor. The preparation method of the polymer and the nano-drug delivery system thereof have the advantagesof easy reaction operation, less reaction steps, short reaction period and high repeatability.

The invention provides a 4-coumarate coenzyme A ligase originated from ornithogalum caudatum, and the amino acid sequence of the ligase is represented by the SEQ ID No.1. The invention further provides a nucleotide sequence, which encodes the gene of the 4-coumarate coenzyme A ligase and is represented by the SEQ ID No.2, a carrier containing the nucleotide sequence and a host cell. The invention also provides an application of 4-coumarate coenzyme A ligase or cells containing 4-coumarate coenzyme A ligase on catalyzing substrates such as p-coumaric acid, caffeic acid, ferulic acid, cinnamic acid, and the like into corresponding thioester compounds.