60 results about "P-Coumaric acid" patented technology

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.

The invention discloses a method for producing p-coumaric acid through double-enzyme cascade catalysis, which comprises the following steps: taking phenol, pyruvic acid and ammonium chloride as substrates, and carrying out cascade enzyme catalytic reaction on a tyrosine phenol lyase product and a tyrosine ammonia lyase product to synthesize p-coumaric acid. According to the method, phenol, pyruvic acid and ammonium chloride are used as substrates, a tyrosine phenol lyase product and a tyrosine ammonia lyase product are used as catalysts, the tyrosine phenol lyase product can convert the substrates into L-tyrosine, and the tyrosine ammonia lyase product can directly use the L-tyrosine as the substrate to convert the L-tyrosine into p-coumaric acid. Therefore, the tyrosine phenol lyase product and the tyrosine ammonia lyase product can realize cascade enzyme catalytic reaction in the same reactor, phenol with lower cost can be adopted as a conversion substrate, separation and purification of an intermediate product L-tyrosine are avoided, and the whole process flow is simple and efficient.

The invention discloses an apigenin derivative as well as a preparation method and application thereof. The apigenin derivative has the following molecular structure expression, and the preparation method comprises the following steps: 1) in the presence of an optional catalyst, reacting p-coumaric acid with ethyl sulfonyl chloride to generate an intermediate 1; and 2) mixing the purified intermediate 1 with oxalyl chloride for reaction, carrying out acylating chlorination, adding apigenin, and carrying out stirring reaction in the presence of an acid-binding agent to obtain the apigenin derivat.The apigenin derivative is novel in structure and has good biological activity for reducing the blood uric acid level in vivo.

The application discloses a kind of compound Xianghua granules fingerprint's construction method, comprising the following steps: preparation of reference solution: p-coumaric acid, ferulic acid standard in methanol is dissolved;Preparation of test solution: different batches of compound Xianghua granules are dissolved in ultrapure water respectively, and post-processing is carried out;Respectively inject high performance liquid chromatography and detect, obtain the fingerprint of reference solution and the fingerprint of each batch sample;Using traditional Chinese medicine fingerprint similarity evaluation system analyzes the fingerprint of each batch sample, generates the control fingerprint of compound Xianghua granules.The method provided by the application can better reflect the multiple index component information of compound Xianghua granules.The fingerprint characteristic peak baseline of the application is smooth, and the sensitivity and separation degree are better, while the operation is quick and reliable, the cost is lower, can effectively, stably and quickly comprehensively monitor product quality, ensure the stability and quality controllability of product.

The invention discloses a Rogowski eutrophy alkali-producing engineering strain for producing p-coumaric acid as well as a construction method and application of the Rogowski eutrophy alkali-producing engineering strain. The preparation method comprises the following steps: by taking a chemoautotrophic microorganism Ropriavidus alcaligenes H16 as an original strain, firstly, by knocking out a synthetic route of a strain poly-3-hydroxybutyrate (PHB), introducing a tyrosine ammonia lyase gene from Rhodotorula glutinis to construct a basic engineering strain capable of producing p-coumaric acid, and then, carrying out fermentation on the basic engineering strain to obtain the p-coumaric acid. Then, endogenous 3-deoxy-7-phosphoheptanone acid synthase gene aroG1 and chorismate synthase gene aroC are overexpressed, and the total copy is increased, so that the p-coumaric acid synthetic pathway flux of the engineering strain is enhanced; and introducing NAD (P) transhydrogenase gene pntAB from Escherichia coli to improve the supply of cofactors, so as to finally obtain the eutrophy alcaligenes Rosei engineering strain capable of synthesizing p-coumaric acid by using CO2.

This invention relates to the fields of synthetic biology and biochemical engineering, and provides an engineered *Yersinia lipolytica* strain that enhances coumaric acid accumulation, its construction method, and its applications. The engineered *Yersinia lipolytica* strain of this invention uses *Yersinia lipolytica* as the substrate strain, expresses the tyrosine ammonia-lyase encoding gene RgTAL derived from *Rhodotorula glutinis*, and completely knocks out the aminotransferase encoding gene containing the nucleotide sequence shown in SEQ ID NO 1 (database number YALI0A08734g). RgTAL converts endogenous L-tyrosine to coumaric acid. This invention reduces dependence on complex metabolic regulation and increases the coumaric acid accumulation level in *Yersinia lipolytica*.

A MATZHU is prepared by using leaves of Gramineae (Graminae) and Bambusoideae plant as raw materials, and has a stable emerald color, and has an average powder particle size of 800 to 10,000 meshes, and has a total amount of dietary fiber of ≥60%, a content of lignin of ≥20% and a content of minerals of ≥7%, and comprises at least three or more bamboo leaf characteristic components, such as orientin, isoorientin, vitexin, isovitexin, adenosine, δ-hydroxylysine and p-coumaric acid. The method for the MATZHU preparation comprises, in turn, performing blanching and color protection, drying and superfine grinding the raw materials. By utilizing the thermal stability and the light stability of the MATZHU, the MATZHU may be used as a raw food material, a functional ingredient, or a dietary supplement.

According to the genetically engineered bacterium for high yield of p-coumaric acid and the application of the genetically engineered bacterium, tyrosine ammonia lyase from different sources and expression vectors with different copy numbers are combined to optimize metabolic pathways, so that the yield of p-coumaric acid is increased. The method specifically comprises the following steps: constructing recombinant expression vectors by using tyrosine ammonia lyase FjTAL from flavobacterium johnsonii and tyrosine ammonia lyase FsTAL from flavobacterium cissii and expression vectors pACYCDuet-1, pCDFDuet-1, pETCDuet-1 and pRSFDuet-1 with different copy numbers respectively by using a homologous recombination technology, transferring the recombinant expression vectors into escherichia coli BL21 (DE3) to obtain engineering strains pCA1-pCA8, and carrying out fermentation on the engineering strains pCA1-pCA8 to obtain the tyrosine ammonia lyase FjTAL from flavobacterium johnsonii and the tyrosine ammonia lyase FsTAL from flavobacterium cissii. Glucose is used as a substrate for fermentation and screening to obtain the high-yield engineering bacterium pCA8, and the yield of p-coumaric acid of the engineering bacterium pCA8 reaches 251.61 mg / L. By optimizing key enzyme expression, tyrosine is efficiently promoted to be converted into p-coumaric acid, and the method has the advantages of being low in cost, high in efficiency, environmentally friendly and the like, is suitable for industrial production and can be widely applied to the fields of biomedicine, food, cosmetics and the like.

The present application belongs to the field of livestock and poultry breeding and feed processing technology, and particularly relates to a feed additive, a nutritional supplement, a feed and application thereof. The present application provides application of p-coumaric acid in improving carcass and meat quality, improving feed conversion rate, reducing fat deposition of livestock and poultry and improving fat metabolism of livestock and poultry. The results of the examples show that p-coumaric acid can effectively improve the feed conversion efficiency of growing pigs in the growth stage, which provides a reference for reducing feed waste in production and realizing cost reduction and benefit increase of animal husbandry; under the premise of not reducing or even improving the growth performance and slaughter rate of growing and fattening pigs, the p-coumaric acid can reduce the fat thickness, improve the pig carcass rating, improve the meat quality, and is beneficial to realize efficient production of high-quality pork and improve the market competitiveness of pork in China; the p-coumaric acid can also reduce the blood lipids of chickens, reduce fatty liver and reduce subcutaneous fat deposition, maintain normal metabolism of chickens and improve the health of chickens.

The invention provides a herbicide for preventing and treating cyperus rotundus in cooperation with glyphosate. The herbicide is prepared from bacillus velezensis fermentation liquor, phenolic acid and glyphosate. The phenolic acid comprises any one or a combination of more of p-coumaric acid, cinnamic acid, p-hydroxybenzoic acid and caffeic acid. The bacillus velezensis fermentation liquor and phenolic acid components are introduced and form a synergistic effect with the glyphosate, so that the dosage of a single dosage of the glyphosate is reduced, the control effect is guaranteed, meanwhile, the potential pressure of chemical pesticide input on the ecological environment is reduced, and the environmental compatibility of a weeding system is improved.

According to the tyrosine ammonialyase mutant and the application thereof, 28 amino acids at the N end of tyrosine ammonialyase are deleted or replaced with 25 or 12 amino acids to obtain the mutant, the enzyme activity is effectively improved by 13.96-27.83% compared with that of a wild type, meanwhile, the coumaric acid biosynthesis efficiency is improved, by-product accumulation is reduced, and the yield of the tyrosine ammonialyase mutant is improved. And industrial application of p-coumaric acid biosynthesis is promoted.

This invention discloses a method for preparing and applying a coumaric acid-modified MOF nanopesticide loaded with chlorantraniliprole, belonging to the field of pesticide formulation technology. The nanopesticide comprises: an iron-based metal-organic framework carrier MIL-101(Fe); chlorantraniliprole loaded within the pores of the carrier; and coumaric acid modified on the surface of the carrier through electrostatic interactions. The preparation method includes three steps: MIL-101(Fe) carrier synthesis, chlorantraniliprole loading, and coumaric acid surface modification. The process is simple and the conditions are mild. This invention utilizes the alkaline environment of the fall armyworm's gut to trigger carrier degradation, achieving targeted and controlled release of active molecules. Simultaneously, the released MIL-101(Fe) is reduced under the action of GSH in the gut, generating reactive oxygen species through the Fenton reaction, forming a synergistic insecticidal effect with coumaric acid. Experiments have shown that this nanopesticide exhibits far superior insecticidal activity against fall armyworm compared to single agents or carriers, possessing excellent insecticidal activity and environmental compatibility, making it suitable for green agricultural production.

The application discloses an engineered strain for synthesizing resveratrol by using p-coumaric acid as a substrate, construction and application thereof, and belongs to the technical field of biotechnology. The active sites of 4-coumaroyl-CoA ligase and resveratrol synthase are semi-rationally designed and modified, the optimal mutants 4CL1 (M4) and VST1 (N3) are expressed in a Yarrowia lipolytica strain, and the obtained engineered strain can efficiently convert p-coumaric acid into resveratrol. The yield of resveratrol in a shake flask fermentation reaches 724.6 mg / L. Then, the two mutants are fused and expressed by using a rigid linker, and the Yarrowia lipolytica engineered strain for producing resveratrol by using p-coumaric acid as a substrate is obtained. In a 5-L fermenter, batch feeding fermentation is carried out, the substrate conversion rate reaches 92.3%, and the yield of resveratrol reaches 35.8 g / L.

This invention discloses a strain that degrades various lignin-derived aromatic compounds and its application in detoxification of hydrolysate. The strain is classified as *Rhodococcus ethereans*. Rhodococcus ether-eating Strain N1, deposited at the China Center for Type Culture Collection (CCTCC) on August 11, 2022, with accession number CCTCC NO: M 20221270, can degrade p-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillin, coniferyl alcohol, and syringaldehyde. After lignin depolymerization, derivative phenolic inhibitors are formed, mainly divided into three types: S-type (syringaldehyde), G-type (ferulic acid), and H-type (p-coumaric acid), which affect the fermentation of the strain. The Rhodococcus bacterium in this invention can efficiently degrade SGH-type monomers, achieving a removal efficiency of up to 68.4% when applied to the biological detoxification of hydrolysate inhibitors. Strain N1 has significant application value in improving the conversion efficiency of lignocellulose hydrolysate and reducing the detoxification cost in industrial production.

The application discloses a hypoglycemic and hypolipidemic composition from the leaves of pandamus, a preparation method and application thereof. The composition contains p-coumaric acid, rutin, kaempferol-3-O-rutinoside, pandamarilactonine-A, pandamarilactone-1 and pandamarilactone-2. The composition with a preferred mass ratio has a certain synergistic effect. Under the premise of a consistent total dose, the composition is superior to p-coumaric acid, rutin and kaempferol-3-O-rutinoside in improving insulin resistance and reducing fat deposition, and is also superior to a pandamus alkaloid composition composed of pandamarilactonine-A, pandamarilactone-1 and pandamarilactone-2, which indicates that there is a synergistic effect among the components of the composition.

The invention provides a honey variety identification method based on amino acid and polyphenol compounds and application of the honey variety identification method, and relates to the technical field of honey variety detection. According to the invention, amino acids and polyphenol compounds in 14 honey varieties are analyzed by adopting a PLS-DA analysis method, and characteristic difference indexes with VIP values greater than 1 are screened out, specifically trans-cinnamic acid, protocatechuic acid, Pro, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 13, 13, 13, 13, 13, 13, 13, 13, 13, 13, 13, 13, 14 According to the method, 2, 4-dimethylcinnamic acid, rosmarinic acid, apigenin, Tyr, vanillic acid, cumaric acid, isoferulic acid, Cys, caffeic acid phenethyl ester, Met, Asp, gallic acid, naringenin, p-cumaric acid, ferulic acid, rhinoceroic acid, chrysin and syringic acid are screened, and then screened characteristic difference index data is used for establishing a linear discrimination model and a random forest prediction model. Through verification, the linear discrimination model and the random forest prediction model constructed by the method can be accurately and efficiently used for discriminating honey varieties.

A nanomaterial is gold or silver nanoparticle having a nanoparticle surface that consists of one or more of the following extracts from sweet gum leaves: shikimic acid, sweet gum leaf family of Catechins, sweet gum leaf family of Quercetin, Sweroside, Afzelechin, p-Coumaric Acid, Epiafzelechin Trimethyl Ether, Myricetin, Naringenin, Phloretin, Procyanidin B1 and related polyphenols, flavonoids and alkaloids. An antiviral / antibacterial agent includes sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral / antibacterial exceeding a 3 log 10 reduction in viral titer.

The application discloses a Yarrowia lipolytica strain for synthesizing (2S)-naringenin and application thereof, and belongs to the field of genetic engineering and bioengineering technology. The application improves the yield of (2S)-naringenin by 60% through semi-rational modification of a key enzyme CHS, and further integrates the modified CHS into the yeast genome. In addition, the application promotes the conversion of coumaric acid to (2S)-naringenin by strengthening the shikimic acid pathway and enhancing the supply of auxiliary factors, so that the yield of (2S)-naringenin reaches 776 mg / L after 96 hours of fermentation of the constructed strain in a 24-hole plate, and the yield reaches 8.65 g / L in a 5L fermenter. The application lays a foundation for subsequent biosynthesis of flavonoids, and has potential value and significance for the development of synthetic biology.

The present invention relates to an anti-inflammatory extract of soil microorganisms or a fraction thereof, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria. The invention further relates to compositions comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS. The invention further relates to pharmaceutical compositions comprising the anti-inflammatory extract of soil microorganisms or a fraction thereof or a composition comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS as an active ingredient. The invention further relates to an anti-inflammatory extract of soil microorganisms or a fraction thereof or a pharmaceutical composition comprising an anti-inflammatory extract of soil microorganisms or a fraction thereof or a composition comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS as an active ingredient for use as a medicament, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria. The invention further relates to compositions comprising an anti-inflammatory extract of soil microorganisms or a fraction thereof, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria.

The invention provides a composition comprising oleanolic acid and the salts, prodrug and solvates thereof; and one or more compounds selected from the following group: ellagic acid, adenine, betaine, choline, miquelianin, p-coumaric acid, pipecolic acid, protocatechuic acid and tyramine, and the salts, prodrugs and solvates thereof. Such a composition is for use in reducing lipid levels in sebocytes, and the composition may find use in treating acne vulgaris and rosacea.

The application discloses a method for constructing a compound health-care wine fingerprint spectrum, which comprises the following steps: preparing a test sample solution, preparing a control sample solution, performing multi-index quantitative determination, determining the fingerprint spectrum, and performing fingerprint spectrum analysis and processing. The method can simultaneously detect 21 characteristic fingerprint peaks in the compound health-care wine, and 4 fingerprint peaks are identified by comparison with the control sample, which are chlorogenic acid, bitter crocins, p-coumaric acid, ferulic acid and the like; and more fingerprint spectrum characteristic peaks can be obtained in the shortest possible separation time.

The invention discloses a method for rapidly determining various phenolic acid substances in a fermentation product, and belongs to the technical field of detection. The determination method comprises the following steps: 1. Taking a to-be-detected substance, and extracting phenolic acid substances in the to-be-detected substance through an alcohol precipitation-SAX solid-phase extraction combined technology to obtain a to-be-detected sample; analyzing the to-be-detected sample by adopting ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry to obtain the content of the target substance in the to-be-detected substance; the target substance is a non-volatile phenolic acid substance. Accurate quantification of trace and trace caffeic acid, dihydrocaffeic acid, ferulic acid, chlorogenic acid, gallic acid, vanillic acid, syringic acid, p-coumaric acid, salicylic acid and (+ / -)-catechin in fermentation products within 8-15 min is achieved by combining impurity removal and concentration pretreatment with ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry. According to the method, the pretreatment mode is convenient to operate, the detection process is fast, and the detection result is accurate.

The application discloses application of OPR-7S protein and an encoding gene thereof in regulation of lodging resistance of wheat. The application firstly constructs OPR-7S super-expression trans-OPR-7S wheat, and then analyzes the lodging resistance and yield-related traits of the trans-OPR-7S wheat and wild-type tetraploid wheat Kronos, finds that OPR-7S can regulate the lodging resistance of the wheat and does not affect the yield-related traits. Further detection of the content of coumaric acid and ferulic acid in the trans-OPR-7S wheat and the wild-type tetraploid wheat Kronos finds that OPR-7S can improve the lodging resistance of the wheat by regulating the content of the coumaric acid and the ferulic acid in the cell wall of the wheat. The OPR-7S protein and related biological materials will play an important role in cultivation of lodging-resistant wheat varieties.

The present application relates to the technical field of bioengineering, and in particular to a kind of immobilized tyrosine ammonia lyase genetically engineered bacteria microbial cell and its preparation and application.The wet bacteria body is mixed with Gly-NaOH buffer solution first, to obtain bacterial suspension;The wet bacteria body is the wet bacteria body of tyrosine ammonia lyase containing engineering bacteria;Then hydroxylated modified diatomite is mixed with bacterial suspension, to obtain mixture;Finally, PEI aqueous solution is added in the mixture for once crosslinking treatment, then glutaraldehyde is added for twice crosslinking treatment, and the immobilized tyrosine ammonia lyase genetically engineered bacteria microbial cell is obtained after treatment.The immobilized tyrosine ammonia lyase genetically engineered bacteria microbial cell is recycled in the present application, and the substrate conversion rate is more than 70% when repeated reaction is 20 batches, and the catalyst still retains more than 70% of initial enzyme activity.The immobilized catalyst has the industrial application potential of biocatalytic production of p-coumaric acid.

The invention provides an application of an HXT4 gene or an HXT4 protein in improving the tolerance of saccharomyces cerevisiae to a hydroxycinnamic acid compound. The recombinant plasmid containing the HXT4 gene is transferred into the saccharomyces cerevisiae, so that the tolerance concentration of the saccharomyces cerevisiae to hydroxycinnamic acid compounds such as coumaric acid and ferulic acid can be increased from 1 mg / mL to 10 mg / mL, that is, overexpression of the HXT4 gene in the saccharomyces cerevisiae can significantly improve the tolerance of the saccharomyces cerevisiae to the hydroxycinnamic acid compounds; the purpose of adding more hydroxyl cinnamic acid compounds into products (such as medicines, health-care products, cosmetics or foods) containing the saccharomyces cerevisiae is achieved, and the quality of the products is further remarkably improved.

The invention discloses an antibacterial and comfortable spandex fabric. The spandex fabric is prepared by dipping spandex yarn into an antibacterial solution containing an antibacterial composition, irradiating the fabric with ultraviolet light to bond effective antibacterial components to the spandex yarn, and finally spinning and weaving the fabric to obtain the antibacterial and comfortable spandex fabric. The spandex yarn is a polyurethane block copolymer obtained by polycondensing polytetramethylene ether glycol and isophorone diisocyanate with ethylenediamine as a chain extender. The antibacterial composition comprises the following raw materials in parts by weight: 5-10 parts of a p-coumaric acid derivative, 15-20 parts of a surfactant, 1-3 parts of menthol, and 1-3 parts of sodium carboxymethyl cellulose. The prepared spandex fabric has a smooth hand feel, is soft and comfortable, has good antibacterial performance, and has good durability. The spandex fabric can be applied to the fields of sportswear, underwear fabrics, socks fabrics, and the like.

The present invention discloses a method for biosynthesizing compounds using lignocellulose derivatives, comprising the following steps: A. Modifying Escherichia coli to obtain a biocatalyst; B. Using lignocellulose derivatives as starting materials to synthesize compounds through the biocatalyst; the lignocellulose derivatives include at least one of p-coumaric acid and ferulic acid; the compounds include at least one of gastrodin, arbutin, salidroside and their derivatives hydroquinone, tyrosol, hydroxytyrosol and homovanillyl alcohol. The present invention modifies Escherichia coli by genetic engineering means, constructs 3 new enzymatic reaction pathways, and uses lignocellulose-derived aromatic compounds p-coumaric acid ( p -coumaric acid) and ferulic acid to efficiently synthesize various high-value-added compounds including gastrodin, arbutin, salidroside, etc., and the product yields all reach grams or more.

The invention provides a recombinant saccharomyces cerevisiae strain for improving the yield of aromatic acid as well as a construction method and application of the recombinant saccharomyces cerevisiae strain. A saccharomyces cerevisiae strain PC01 for synthesizing protocatechuic acid and p-coumaric acid is used as a chassis, fbp1, pfk1, pfk2 and shb17 are knocked out, and an endogenous escape path and invalid cycle of saccharomyces cerevisiae are blocked. Meanwhile, a p-coumaric acid synthesis pathway is knocked out, so that the inhibition of p-coumaric acid on the growth of the recombinant saccharomyces cerevisiae is relieved. Then, overexpressing a protocatechuic acid synthetase gene so as to enhance the metabolic flux of the protocatechuic acid, and overexpressing Aro3D154N, Aro4K229L, EcAroB and EcAroD so as to enhance the synthesis of the main carbon flow to the protocatechuic acid. And finally, integrating other aromatic acid metabolic pathways to realize biosynthesis of salicylic acid, p-hydroxybenzoic acid and gallic acid. And the universality of the engineering strain is verified. The recombinant strain provided by the invention takes glucose as a carbon source, so that not only is the production cost reduced, but also the stable hereditary character can be maintained, and the high yield of aromatic acid is ensured.

This invention belongs to the field of genetic engineering, and specifically relates to a 4-coumaric acid-coenzyme A ligase gene Gl4CL from *Gynostemma pentaphyllum*, its encoded product, and its applications. This invention is the first to clone and prepare the 4-coumaric acid-coenzyme A ligase gene Gl4CL from the medicinal plant *Gynostemma pentaphyllum*. The nucleotide sequence of this gene is shown in SEQ ID NO.1, and the amino acid sequence of the product encoded by this nucleotide sequence is shown in SEQ ID NO.2. The 4-coumaric acid-coenzyme A ligase gene Gl4CL is a key regulatory gene in the flavonoid biosynthesis pathway of *Gynostemma pentaphyllum*. This enzyme can be used in the preparation of p-coumaryl-CoA using p-coumaric acid as a substrate, further promoting the biosynthesis of flavonoid compounds. This invention can provide an effective method to meet the huge market demand for flavonoid compounds.

The invention belongs to the field of microbial metabolic engineering, and discloses recombinant saccharomyces cerevisiae for synthesizing p-coumaric acid by using acetic acid as well as a preparation method and application of the recombinant saccharomyces cerevisiae. The recombinant saccharomyces cerevisiae has the following characteristics that: tyrosine lyase FjTAL, phenylalanine lyase AtPAL2, cinnamic acid 4-hydroxylase AtC4H, cytochrome P450 reductase AtATR2 and cytochrome B5 CYB5 are subjected to overexpression. The invention also provides a construction method and a domestication method of the recombinant saccharomyces cerevisiae. Compared with recombinant saccharomyces cerevisiae which is not evolved, the recombinant saccharomyces cerevisiae evolved adaptively can well grow in a culture medium which takes acetic acid as a unique carbon source, and p-coumaric acid with higher yield can be synthesized. The invention provides a feasible strategy for synthesizing p-coumaric acid, flavonoid or stilbene natural products by using acetic acid as a carbon source.