HPLC separate detection for polyphenols and rhodiola crenulata quality detection method
A detection method, the technology of Rhodiola rosea, applied in the field of high-performance liquid chromatography detection, can solve the problems of limited quantity and difficulty in realizing the quality control of Rhodiola rosea medicinal materials, and achieve the effect of high practicality and applicability
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Embodiment 1
[0020] like Figure 1 ~ Figure 2 As shown, the method of the present invention is used to detect the components of the Rhodiola rosea extract.
[0021] 1. Preparation of the test solution
[0022] Raw materials: take Rhodiola crenulata medicinal material, wash, dry and grind into powder for later use.
[0023] Take about 2.5g of raw medicinal material powder, weigh it accurately, put it in a 50mL measuring bottle, add 20mL of 30% ethanol, ultrasonicate at 20°C (power 250W, frequency 40kHZ) for 20min, let it stand at room temperature, add 30% ethanol to dilute to the mark, shake Evenly, accurately measure 5mL, put it in a 25mL measuring bottle, add 30% ethanol to dilute to the mark, shake evenly, filter, and take the subsequent filtrate to obtain the final product.
[0024] 2. Preparation of reference solution A
[0025] Reference substance: gallic acid, salidroside, tyrosol, catechin, ethyl gallate, p-coumaric acid
[0026] Reference substance solution A: take an appropria...
Embodiment 2
[0040] The components of the Rhodiola rosea extract were detected by the method of the present invention. The same parts as in Example 1 were not repeated, and the difference was in the gradient elution conditions. Gradient elution conditions are shown in Table 2. The chromatograms are shown in image 3 .
[0041] Table 2 gradient elution conditions
[0042]
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