Method for improving efficiency of ferulic acid and p-coumaric acid separation

A technology for p-coumaric acid and separation efficiency, applied in the field of effective utilization of biomass resources, can solve the problems of large amount of solvent, restrictions on the wide application of ferulic acid, and high cost, and achieve the effect of improving extraction efficiency

Inactive Publication Date: 2018-06-15
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It takes a long time to prepare ferulic acid and p-coumaric acid by chemical synthesis, the amount of solvent is large, the conversion rate is low, and the cost is high, which limits the wide application of ferulic acid and p-coumaric acid
At present, one-step alkaline hydrolysis is mainly used to extract ferulic acid and p-coumaric acid, but the extraction effect is poor.
HPLC sample preparation is simple and requires no special treatment, but the separation ability is weak, and it is difficult to separate and detect the dimer of hydroxycinnamic acid produced in the process of alkaline hydrolysis

Method used

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  • Method for improving efficiency of ferulic acid and p-coumaric acid separation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Such as figure 1 Shown, a kind of method that improves ferulic acid and p-coumaric acid separation efficiency, operates as follows:

[0024] 1. After hydrolyzing ball-milled bagasse with NaOH solution, separate solid and liquid A; adjust the pH of liquid A to 5, add ethanol with a volume concentration of 95% to precipitate and separate 12% hemicellulose, obtain liquid B, and remove ethanol by rotary evaporation.

[0025] 2. Adjust the pH of liquid B to 1, and centrifuge to separate 10% lignin to obtain an extract;

[0026] 3. Extract the extract with ethyl acetate, separate the organic phase, remove the water from the organic phase, and spin evaporate to obtain ferulic acid and p-coumaric acid.

[0027] 4. Treat the solid with 100ml of dioxane / hydrochloric acid mixed solution, reflux reaction in an oil bath at 80°C for 3 hours, and separate after cooling to obtain residue 1 and liquid C; wherein, the volume ratio of dioxane / hydrochloric acid is 9:1, HCl concentration ...

Embodiment 2

[0032] Such as figure 1 Shown, a kind of method that improves ferulic acid and p-coumaric acid separation efficiency, operates as follows:

[0033] 1. After hydrolyzing ball-milled bagasse with NaOH solution, separate solid and liquid A; adjust the pH of liquid A to 6, add ethanol with a volume concentration of 95% to precipitate and separate 14% hemicellulose, obtain liquid B, and remove ethanol by rotary evaporation .

[0034] 2. Adjust the pH of liquid B to 1-2, and centrifuge to separate 8% lignin to obtain an extract;

[0035] 3. Extract the extract with ethyl acetate, separate the organic phase, remove the water from the organic phase, and spin evaporate to obtain ferulic acid and p-coumaric acid.

[0036] 4. Treat the solid with 100ml of dioxane / hydrochloric acid mixed solution, reflux reaction in an oil bath at 80°C for 3 hours, and separate after cooling to obtain residue 1 and liquid C; wherein, the volume ratio of dioxane / hydrochloric acid is 9:1, HCl concentrati...

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Abstract

The invention discloses a method for improving the efficiency of ferulic acid and p-coumaric acid separation. According to the method, basic hydrolysis and acid hydrolysis are adopted in sequence to separate ferulic acid and p-coumaric acid from bagasse cell walls, and GC-MS quantitative analysis is carried out on the content of ferulic acid and p-coumaric acid. According to the method, a comprehensive method including basic hydrolysis and acid hydrolysis in sequence is adopted to separate ferulic acid and p-coumaric acid from bagasse cell walls, thereby eliminating interference of hemicellulose and lignin; ethyl acetate is taken as an extraction solvent, thereby obviously improving the yield of ferulic acid and p-coumaric acid; and GC-MS fast efficient quantitative analysis is carried outon ferulic acid and p-coumaric acid. The method establishes the foundation for efficient separation and industrial application of ferulic acid and p-coumaric acid.

Description

technical field [0001] The invention relates to the effective utilization of biomass resources, and in particular provides a method for improving the separation efficiency of ferulic acid and p-coumaric acid. Background technique [0002] Ferulic acid (4-hydroxy-3-methylcinnamic acid) and p-coumaric acid (p-hydroxycinnamic acid) are the major hydroxycinnamic acids in cell walls. During the formation of cell walls of non-wood raw materials, while hydroxycinnamic acid reacts with lignin monomers, it also undergoes esterification reaction with polysaccharides to form structures such as ferulic acid ester and p-coumaric acid ester. These cross-linked structures play an important role in the growth of plant cell walls, can regulate the mechanical properties and biodegradability of cell walls, and act as a "bridge" between lignin and carbohydrates. [0003] Ferulic acid and p-coumaric acid are widely found in the cell walls of lignocellulosic biomass such as agricultural and fore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N30/00G01N30/02G01N30/14C07C51/42C07C59/64C07C59/52
CPCC07C51/42G01N1/34G01N30/00G01N30/02G01N30/14G01N2030/062G01N2030/067C07C59/64C07C59/52
Inventor 闵斗勇李明富贾转万广聪王双飞覃程荣
Owner GUANGXI UNIV
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