Method for preparing p-coumalic acid by using spartina alterniflora

A technology of Spartina alterniflora and p-coumaric acid, which is applied in the separation/purification of carboxylic acid compounds, medical preparations containing active ingredients, and applications, etc., can solve problems such as affecting biodiversity, and achieve remarkable results and yields. High and low cost of planting

Active Publication Date: 2019-03-29
南京施倍泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the spread and excessive expansion of Spartina alterniflora, its negative effects have gradually emerged, affecting the biodiversity of some coastal areas, especially tidal flat aquaculture.

Method used

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  • Method for preparing p-coumalic acid by using spartina alterniflora
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  • Method for preparing p-coumalic acid by using spartina alterniflora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The research of embodiment 1 Spartina alterniflora active ingredient

[0030] Collect 100kg of the aboveground part of Spartina alterniflora, dry it in the sun, cut it into 2-3cm long, add 20 times of hot water to extract, and concentrate the extract to a dark brown liquid (about 10kg).

[0031] The technical solution disclosed in the patent application number 201510266409.2 can also be referred to to prepare the fine powder of Spartina alterniflora, which is dissolved in pure water and configured as a 1:10 sample mother liquor (aqueous solution, wherein the total saponin content is > 6.0%). Spartina powder used in the present invention was purchased from Jiangsu Hailifa Biotechnology Co., Ltd.

[0032] Take 2500g of concentrated solution of Spartina alterniflora, extract and extract 3 times with an equal volume of ethyl acetate, take the organic phase and concentrate it by rotary evaporation under reduced pressure to obtain 113.8g of crude paste of the ethyl acetate ph...

Embodiment 2

[0036] Example 2 Animal Test of Spartina alterniflora Extract and p-coumaric Acid for Lowering Blood Uric Acid

[0037] 1. Experimental materials

[0038] 1. Animals: 20-25 grams of male Kunming mice, 60

[0039] 2. Modeling agent: hypoxanthine

[0040] 3. Positive drug: benzbromarone

[0041] 4. Pseudopositive drugs: Spartina powder, desugared Spartina powder

[0042] 5. Negative agent: normal saline

[0043] 6. Test agent: p-coumaric acid isolated and identified from Spartina extract

[0044] 7. Solvent: sodium carboxymethylcellulose-CMC-Na

[0045] 8. Syringe: 1ml, 5ml

[0046] 9. Centrifuge tube: 1.5ml, 2ml, 5ml

[0047] 10. Capillaries for blood collection

[0048] 11. Feeder: flat head 9# needle

[0049] 2. Test equipment

[0050] Beckman LX20 automatic biochemical analyzer

[0051] 3. Experimental plan

[0052] 1. Reagent preparation:

[0053] (1) Preparation of 0.8% CMC-Na (sodium carboxymethyl cellulose): Weigh 2.4 grams of CMC-Na, first adjust it into a ...

Embodiment 3

[0113] Example 3 Comparison test of p-coumaric acid prepared by the method of the present invention and commercially available products for reducing blood uric acid

[0114] Referring to the method of Example 2, the blood uric acid-lowering effect of p-coumaric acid prepared by the method of the present invention and commercially available p-coumaric acid (purchased from Shanghai Yuanye Biotechnology Co., Ltd.) was investigated, and the results are shown in Table 10.

[0115] Both the p-coumaric acid 0.5mg / ml dosage group and the commercially available p-coumaric acid group 0.75mg / ml dosage group prepared by the present invention can reduce the serum uric acid level of mice with hyperuricemia, while the commercially available p-coumaric acid group The same dose of 0.5 mg / ml group could not reduce the serum uric acid level of mice with hyperuricemia (Table 10). This shows that the activity of the present invention on reducing the serum uric acid level of mice with hyperuricemia...

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Abstract

The invention discloses a method for preparing p-coumalic acid by using spartina alterniflora. Spartina alterniflora extraction liquid is subjected to ethyl acetate extraction; then, water phases aretaken; next, n-butyl alcohol is used for extraction; n-butyl alcohol phase extraction liquid is concentrated to obtain n-butyl alcohol phase crude paste; the n-butyl alcohol phase crude paste is sequentially subjected to positive phase silica gel column chromatography, ODS reversed phase chromatography and gel column chromatography; finally, separation and purification are performed through a highperformance liquid phase chromatography technology, thus obtaining the p-coumalic acid. Pharmacological research results show that the p-coumalic acid has obvious uric acid reduction and blood sugarreduction effects on mice with hyperuricemia.

Description

technical field [0001] The invention belongs to the technical field of plant source functional products. It specifically relates to a method for preparing p-coumaric acid by using Spartina alterniflora and the use of the p-coumaric acid prepared by the method in the preparation of functional products for reducing blood uric acid. Background technique [0002] The chemical name of p-coumalic acid is p-hydroxycinnamic acid, which is the most important kind of hydroxycinnamic acid compounds. Studies have shown that p-coumaric acid has a variety of physiological functions, such as anti-oxidation, anti-bacterial, anti-cardiovascular disease, anti-mutation and other activities. At present, the methods for producing p-coumaric acid include chemical synthesis, natural extraction and biotransformation. The chemical synthesis method is to obtain p-coumaric acid through the reaction of p-hydroxybenzaldehyde and diacid. Most natural extraction methods obtain p-coumaric acid by alkali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C51/47C07C51/48C07C59/52A61P19/06A61P3/10A61K31/192A23L33/105
CPCA23L33/105A23V2002/00A61K31/192A61P3/10A61P19/06C07C51/42C07C51/47C07C51/48C07C59/52A23V2200/306A23V2200/328A23V2250/02
Inventor 钦佩张鹤云
Owner 南京施倍泰生物科技有限公司
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