Method for ecologically interplanting sarcandra glabra under phyllostachys edulis forest

A technology for coral and moso bamboo, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of low maturity rate and harvest rate of grass coral seeds, seasonal restrictions on materials, difficult seed sources, etc., and achieves simple and easy breeding. , Conducive to management and improve the effect of land utilization

Inactive Publication Date: 2013-06-12
浙江慧普农业科技有限公司
View PDF1 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Publication number is that the Chinese patent of CN 101194593A has introduced a kind of tissue culture fast propagation method of Coral praecox, this method uses the root system of aseptic seedling as explant to obtain regenerated seedling through the callus approach, but this method must use Coral pratense without The test-tube seedlin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1:

[0031] (1) Group breeding seedlings

[0032] Choose healthy and disease-free young leaves of Coral Coral as explants, soak them in 75% alcohol for 20 seconds in a clean bench, then soak them in 0.1% mercury solution for 1 min, rinse with sterile water 3 to 4 times, and cut the area. About 1cm 2 The small squares on the left and right are spare. The sterilized leaves were placed on the adventitious shoot induction medium with the paraxial side up, and 5 days after the inoculation, they were transferred to a fresh medium and cultured for another 30 days. Adventitious bud induction medium is based on MS as the basic medium, plus 2.5 mg / L TDZ, 0.15 mg / L NAA, 120g / L banana puree, and 0.8g / L activated carbon; 15 days after the leaves are inoculated to the fresh medium, the cut is slightly There are swelling and green bud spots, and then the regeneration of adventitious buds begins. When cultured for 30 days, the regeneration frequency of explants and the average nu...

Example Embodiment

[0043] Example 2:

[0044] (1) Group breeding seedlings

[0045] Choose healthy and disease-free young leaves of Coral Coral as explants, soak them in 75% alcohol for 20 seconds in a clean bench, then soak them in 0.1% mercury solution for 1 min, rinse with sterile water 3 to 4 times, and cut the area. About 1cm 2 The small squares on the left and right are spare. The sterilized leaves were placed on the adventitious shoot induction medium with the paraxial side up, and 5 days after inoculation, they were transferred to fresh medium and cultured for 35 days. Adventitious bud induction medium is MS as the basic medium, plus 2.0 mg / L TDZ, 0.1 mg / L NAA, 150g / L banana puree and 0.5g / L activated carbon; 17 days after the leaves are inoculated to the fresh medium, the cut is slightly There are swelling and green bud spots, and then the regeneration of adventitious buds begins. When cultured for 30 days, the regeneration frequency of explants and the average number of regenerated adve...

Example Embodiment

[0054] Example 3:

[0055] The operation steps and parameters of this example refer to Example 1. The difference is: the leaf adventitious bud induction medium is MS as the basic medium, plus 4.0 mg / L TDZ, 0.2 mg / L NAA, 100g / L banana puree and 1.0g / L activated carbon; 15 days after the leaves were inoculated to fresh medium, the incision was slightly enlarged and green bud spots appeared, and then adventitious buds began to regenerate. When cultured for 30 days, the regeneration frequency of explants and the average number of regenerated adventitious buds were 78.4% and 4.7, respectively. The adventitious buds growing from the edge of the leaves were cut into individual buds and inoculated into a proliferation medium. The proliferation medium was based on MS as the basic medium, plus 2.0 mg / L TDZ and 0.05 mg / L NAA. The same proliferation medium can be used for multiple subcultures, subcultured once every 25 to 30 days, and the average proliferation multiple of cluster buds...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for ecologically interplanting sarcandra glabra under a phyllostachys edulis forest. The method comprises the steps of: (1) tissue culture and seedling raising: sarcandra glabra blades are selected as explants to conduct adventitious bud induction, an adventitious bud induction culture medium uses an MS (Murashige and Skoog) culture medium as a basic culture medium and additionally comprises 2.0 to 4.0mg/L of TDZ (thidiazuron), 0.1 to 0.2mg/L of NAA (naphthyl acetic acid), 100 to 150g/L of banana puree and 0.5 to 1.0g/L of activated carbon, and then adventitious buds are subjected to enrichment culture and rooting culture; (2) seedling hardening: bottom seedlings are conveyed to under the phyllostachys edulis forest to be hardened; (3) transplantation: tissue culture seedlings are transplanted to a north slope bamboo forest with a number of living stems (NLS) of phyllostachys edulis of 150 to 400 plants/mu or a south slope bamboo forest with an NLS of 200 to 400 plants/mu; (4) forest management: the second intertillage is conducted in June and September every year; and (5) harvest: the sarcandra glabra is harvested in November to December every year. By adopting the method, the culture period of the sarcandra glabra can be shortened and the yield of the sarcandra glabra can be increased.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, and specifically relates to a method for ecologically interplanting grass and coral under moso bamboo forests. Background technique [0002] Grass coral (Sarcandra glabra (Thunb.) Nakai), alias: Sarcandra glabra (Thunb.) Nakai, alias: Sarcandra glabra (Thunb.) Nakai, etc., is a perennial evergreen semi-shrub of the genus Sarcandra glabra (Thunb.) Nakai. The whole plant is used for medicine. It has the functions of antibacterial and anti-inflammatory, clearing away heat and detoxification, expelling wind and dampness, promoting blood circulation and relieving pain, promoting menstruation and setting bones, etc. , has a remarkable effect on a variety of malignant tumors, and is an excellent Chinese medicinal material with a wide range of pharmacological effects and low toxicity. With the continuous development of new products that use coral as raw materials, the demand for them is in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01G1/00
Inventor 朱淑颖姜叶琴张海洋
Owner 浙江慧普农业科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap