158results about How to "Breeding is easy" patented technology

The invention discloses a method for culturing osmanthus seedlings, which mainly comprises the following steps of seed treatment, sowing, seedling management and fertilizer water management. The method for culturing osmanthus seedlings has the characteristics of high germination percent, simple and quick propagation, and easy popularization in production. According to the proportion of a matrix prepared by the invention, the matrix has reasonable nutrients and is favorable for quickly propagating seedlings; and as the method of foliar fertilization is used, the nutrients of the seedlings are increased and the propagation time of the seedlings is shortened.

The invention discloses a Bacillus amyloliquefaciens subsp. plantarum ZFH-3 and application thereof. The classification designation of the strain is Bacillus amyloliquefaciens subsp. plantarum, and the collection number is CGMCC No.9884. The strain has obvious inhibiting actions on multiple crop pathogens, especially can effectively control postharvest diseases of fruits, is well used for biological control of contact mold of citrus fruit, olive green mould of ditrus fruit, citrus phomopsis stem end rot, sour rot of citrus, anthracnose of citrus, anthracnose of banana, spike rot of banana, gray mold of strawberry, soft rot of strawberry and other pathogen fungi, obviously lowers the possibility of postharvest diseases of fruits, prolongs the storage life and shelf life, and reduces the consumption of chemical reagents. The strain has the advantages of simple culture conditions and high reproduction speed, can easily implement industrial production, popularization and application, and has favorable development and application prospects.

The invention relates to a method for improving stock grafting through scion and belongs to the technical field of biological engineering. The method comprises the following steps: 1) cultivating a stock: choosing a robust seedling of a plant having subterraneous stems, tubers, and roots as the stock; 2) scion: choosing an excellent foliage branch of the same crop as scion; and 3) grafting. By using the method for improving stock grafting, the mutual effect of scion and stock is utilized, thereby taking full advantages of the stock that the seedling life is long, the root grows well, the capability of absorbing water and fertilizer is strong, the stress resistance is strong, the cultivation management is simple, the growing power is high, the seedling is easily propagated, and the like. The advantages of scion photosynthesis and secondary metabolite composition are fully taken; the quality and output of subterraneous stems, tubers, and roots of the improved stock can be enhanced by scion plant, thereby being high in application value for increasing the output and quality of the root, stem or tuber plant crops. The method for improving stock grafting can be conveniently operated and has the advantages of low cost, high efficiency and stability, accuracy, high speed, and the like.

The invention relates to a multi-strain microorganism pig feed additive and a production method thereof. According to the additive, bacillus subtilis, lactobacillus delbrueckii, saccharomyces cerevisiae, candida and photosynthetic bacteria are inoculated into a fermentation culture medium according to a ratio of 2:1:1:1:2, and are prepared into a finished product through solid fermentation. The microorganism preparation is reasonably matched, various microorganisms in the preparation can bring respective effects into play and depend on one another, the overall advantage of the microorganism preparation can be brought into play, the growth development and the improvement of the anti-disease capability of a host are facilitated, and the feed additive is safe to use and can improve the immunity of animals, prevent and resist diseases, prompt animal growth, improve the feed utilization rate and improve the feed quality.

The invention discloses a method for jointly repairing vegetation and application thereof. The method comprises the steps of: (1) planting shrubs; (2) covering soil, and sowing seedlings of resistant herbaceous plants or planting seedlings of herbaceous plants; and (3) sowing moss. The method has low cost and remarkable effect, can avoid or control the pollution of heavy metal, can avoid and fix sands or conserve water and soil, and plays an important part in protecting environment.

A process for eliminating the pollution of Pb, Zn and Cd to said and water by use of arabis paniculata features that the Arabis paniculata is planted in the polluted sol or water for absorbing Pb, Zn and Cd. When their concentrations in the plant reach a certain value, the plant on the ground is cut out and its root is retained for growing the plant no the ground and further cutting it out.

The invention relates to a bacillus amyloliquefaciens plant subspecies ZFH-3 microbial agent and application thereof and belongs to the field of plant protection and disease prevention and treatment. The microbial agent per g contains 2*10<10>-1.2*10<11>cuf bacillus amyloliquefaciens plant subspecies ZFH-3 living spores, and the ZFH-3 strain is classified and named Bacillus amyloliquefaciens subsp.Plantarum, wherein the collection number is CGMCC No. 9884. The agent has a remarkable inhibiting effect on various pathogens of crops, shows a relatively good preventing and treating effect on prevention and treatment of soil-borne diseases and can effectively prevent and treat soil-borne diseases of vegetables, so that the loss is damaged and the field efficacy is over 59%. The microbial agent is non-toxic and safe to human bodies, replaces chemical agents for preventing and treating soil-borne diseases, conditioning soil and promoting plant growth, and has a good application prospect.

The invention relates to a method for cultivating cross rice. Wherein, it comprises that (1), selecting the light-temperature sensitive sterile system with low-temperature sterile or short-light low-temperature sterile; (2), selecting the sterile masculinity as gene system; (3), crossing sterile system S with sterile masculinity system S', to obtain stable sterile masculinity S / S' F1; (4), screening and recovering system R; (5), using S / S' F1 as mother type to be crossed with recover system R, to find the needed cross combination, to produce cross rice. The invention can improve the match property of sterile system and reduce produce cost.

The invention discloses a Tilia miqueliana seedling raising method, which includes seed collection, drying in the shade, soaking, multi-substrate variable temperature stratification and transplanting. Seedlings of Tilia miqueliana raised by the method break through dormancy in about 50 days, are high in survival rate and emergence percentage, and have developed roots.

The invention discloses a preparation method of varicella-zoster virus glycoprotein E extracellular domain protein, and relates to a recombinant vector of the varicella-zoster virus glycoprotein E extracellular domain protein, a transformant prepared by use of the recombinant vector, a protein formed by use of the transformant and application of the protein in the fields of research and development of varicella vaccines, zoster virus vaccines, combined vaccines and other related vaccines containing the protein, and detection of corresponding antigens and antibodies, and the like. A VZV-gE extracellular nucleic acid sequence is cloned and connected with prokaryotic expression vector pET-21b to obtain the recombinant vector by genetic engineering technology, the recombinant vector is transformed into Escherichia coli BL21 (DE3) to obtain the transformant, and the target protein is obtained by inducible expression of the transformant. The expression system can be used for efficient VZV-gE extracellular antigen protein expression, and the expressed VZV-gE extracellular antigen protein has good immunogenicity, and can be used in the fields of research and development of the related vaccines and detection of the corresponding antigens and antibodies.

The invention provides a processing method for removing the bamboo outer skin and the bamboo inner skin of a bamboo tube. The bamboo outer skin and the bamboo inner skin are removed by rotatably cutting the bamboo tube inside and outside, and bamboo joints inside the bamboo tube are removed in advance before rotary cutting. The processing method comprises the following steps that (a) the bamboo tube is prepared; (b) the bamboo joints are removed; (c) the bamboo outer skin is removed in a rotary cutting mode; and (d) the bamboo inner skin is removed in a rotary cutting mode. The processing method for removing the bamboo outer skin and the bamboo inner skin of the bamboo tube has the advantages that (1) the prepared bamboo tube does not have the bamboo outer skin, the bamboo inner skin or the bamboo joints, can be flattened to manufacture a flawless bamboo plate, and can also be laminated to manufacture a laminated bamboo plate for manufacturing bamboo products such as reconsolidated bamboo; and the bamboo outer skin and the bamboo inner skin, with the same thickness, of an irregular bamboo tube can also be removed, the production efficiency is high, energy consumption in processing is low, and work and time are saved; and (2) the bamboo joints are removed by using a twist bit of an inner roller A, then the bamboo tube is driven to rotate through the rotation of an inner compression roller and an outer compression roller, and the bamboo outer skin and the bamboo inner skin are removed through rotary cutters which are tightly close to the two compression rollers. The processing method for removing the bamboo outer skin and the bamboo inner skin of the bamboo tube provides technical support for manufacturing the bamboo into the flattened bamboo plate and producing the laminated bamboo plate.

The invention discloses a tissue culture method of North American begonia. The tissue culture method comprises the steps of primary culture, subculture, rooting culture and acclimatization for transplant, wherein current-grown tender branches of the North American begonia are taken as explants and are cultured on a primary culture medium for a period of time through sterile inoculation, then the seedlings of the North American begonia on the primary culture medium are cut and then inoculated on a subculture medium for subculture, clustered seedlings growing to be 2cm-3cm are transferred to a rooting culture medium for rooting induction so as to form rooting seedlings, and the rooting seedlings are subjected to hardening and acclimatization to obtain regeneration plants. According to the method, the North American begonia can be bred more easily, a large quantity of sterile seedlings can be obtained in a short time, and the breeding cycle is shortened, so that the planting area can be enlarged rapidly.

The invention relates to a method for promoting growth of plants on manganese tailing residues. Used indigenous bacteria come from the tailing residues on which plants do not grow. Used plants are Phytolacca americana. An inoculated water-retaining gel bacteria bed and the tailing residues are evenly mixed according to a mass ratio being 1: (4-8) to cultivate Phytolacca americana seedlings. The plants can be promoted to grow and breed on the manganese tailing residues to form vegetation. The water-retaining gel bacteria bed contains 5 parts of white mica, 5 parts of montmorillonite, 7 parts of agar and 83 parts of indigenous bacteria culture solution. The indigenous bacteria culture solution contains soluble starch with concentration being 5g / L, beef tallow cream with concentration being 1g / L, peptone with concentration being 3g / L, dipotassium hydrogen phosphate with concentration being 0.2g / L, sodium chloride with concentration being 5g / L, cane sugar with concentration being 3g / L, magnesium sulfate with concentration being 0.2g / L, ferrous sulfate with concentration being 0.2g / L, potassium nitrate with concentration being 0.5g / L, urea with concentration being 10g / L and calcium superphosphate with concentration being 5g / L. The method has the advantages that the operation is simple, the cost is low, the efficiency of resisting and controlling the migration and the diffusion of manganese and other harmful metal in manganese tailing residues, the environment can be beautified and the like.

The invention discloses a biotechnology for petroleum desulfurization and denitrification and an application. A mixed microbial agent is prepared from activated geobacillus thermoglucosidasius w-1, mycobacterium goodiii, thermotoga neapolitana and small thermophilic and oil-degraded bacilli according to the volume ratio of 1:1:1:1. The mixed microbial agent reacts with high-sulfur diesel oil and inferior oil slurry in an organic salt solution, and the sulfur content and the nitrogen content of the two kinds of oil before and after the reaction are measured. A production method is simple in process, needed equipment is simple, cost is low, environment friendliness is achieved, produced fuel oil is good in quality, an ideal treatment effect is achieved for the oil, thermophilic microorganisms achieving the simultaneous desulfurization and denitrification function are adopted for achieving simultaneous desulfurization and denitrification of petroleum in the high-temperature environment, the biological desulfurization and denitrification efficiency of fuel oil can be remarkably improved, the environment pollution problems generated after use can be effectively solved, excellent characteristics of industrially-applied bacterial strains are achieved, and the bacterial strains have wide industrial application potential.

The invention discloses a method for performing segmented seedling thinning on millet by use of a herbicide. A first millet kind only resistant to a sethoxydim herbicide, a second millet kind only resistant to a imazethapyr herbicide and a third millet kind both resistant to the sethoxydim herbicide and the imazethapyr herbicide are mixed according to the proportion of a seeding rate (0.8-1.2): (0.8-1.2): (0.8-1.2) to form a mixed millet kind for sowing, and after emergence of seedlings, chemical seedling thinning is realized through praying the corresponding herbicides, wherein one half of the first millet kind only resistant to the sethoxydim herbicide and one half of the second millet kind only resistant to the imazethapyr herbicide are selected and processed by use of a prometryn herbicide, and the millet subjected to processing by use of the prometryn herbicide dies naturally after 2-3 leaf stages after emergence of the millet seedlings. According to the invention, the seedling thinning method is simple, the condition of a too large seedling number or high seedling density does not occur later, the working strength of farmers is greatly mitigated, the number of the seedlings can be flexibly controlled, segmented seedling thinning is realized, and seedling emergency uniformity is guaranteed.

The invention provides a flattening method for bamboo tubes in a high-temperature tank. The flattening method comprises the following technological steps that (1) pretreatment is conducted on the fresh bamboo tubes; (2) the bamboo tubes are stacked; (3) high-temperature self-flattening is conducted on the circular bamboo tubes in the tank; and (4) post-treatment is conducted after bamboo chips are flattened. The fresh bamboo tubs are subjected to rapid heating and heat-moisture treatment through high-temperature steam in the high-temperature saturated steam tank at the temperature of 130-160 DEG C, and the temperature of the bamboo tubes is made to reach the softening point of lignin, cellulose and hemicellulose. In this way, the circular bamboo tubes with saw kerfs are flattened through round bar steel in the bamboo tubes under the dead-weight action of weights such as external iron blocks, the treatment temperature in the bamboo tube flattening process is decreased, the labor intensity in the operating process of workers is relieved, and the severe environment is improved. The manufactured flattened bamboo tubes are better in physical and mechanical performance and other performance, attractive in color and suitable for art work fabrication, furniture fabrication and house decoration. An optimal processing method is provided for the bamboo industry.

The present invention relates to one kind o water processing functional suspended stuffing and its preparation technology, and the functional suspended stuffing is suitable for treatment of various kinds of waste water. Winged plates for stuffing, fixed connecting ring and middle connecting ring are made with polar plastic and connected to form an integral, and powdered functional water processing material is covered onto the surface of the winged plates via hot binding or adhesion with surface adhesive to constitute composite winged plates. The present invention has convenient making, great surface area, high water processing capacity and convenient maintenance with less back flushing needed.

The invention relates to a cultivating method to utilize flax male sterility to hybrid flax, belonging to the field of crop cultivation. Heterosis utilization is an effective way to improve the yield of flax, but the main obstacle in prior suitable heterosis utilization is the lack of excellent flax male sterile line. The invention is characterized in that, excellent temperature sensitive sterile line successfully selected and bred through antibiotic mutation is utilized for hybrid breeding and hybrid production; the cross combination with obvious yield heterosis is bred; the method to produce sterile line at high temperature area and produce hybrid F1 seed together with a restore line at low temperature area is invented. The cultivating method has the advantages of simple propagation, low production cost, wide sterile line restoring sources and obvious heterosis.

The invention discloses a method for remediating lead-contaminated soil or water, which comprises the following steps: (1) collecting Polygonum criopolitanum; (2) planting Polygonum criopolitanum in soil or water; (3) periodically harvesting the Polygonum criopolitanum and part of stolons above the ground or water; (4), continuing to plant and harvest the Polygonum criopolitanum till the lead content of the soil or water reaches a safe standard; and (5) performing pollution-free disposal on the harvested Polygonum criopolitanum. In the method of the invention, the Polygonum criopolitanum is planted in the lead-contaminated soil or water, so extraction can be performed continuously, the remediation process of the soil and water is simplified, and the lead contamination problem of soil can be solved radically. The method of the invention is low in cost and high in maneuverability and avoids damaging the structure of the environmental soil and causing secondary pollution.

The present invention relates to printing and dyeing waste water treating process and features that the printing and dyeing waste water treating process includes the following successive steps: regulating pH value, coagulating air floatation, anaerobic hydrolysis, anoxic treatment, aeration, secondary sludge setting and drying sludge. During the process, the exhaust water after secondary sludge setting is drained to sewer, the sludge after coagulating air floatation is dried and externally transported, the sludge after secondary sludge setting is returned to the aeration step continuously, the excessive sludge is exhausted from the aeration tank to the anaerobic hydrolysis unit for self consumption, and the anaerobic hydrolysis at pH 6.5-8 generates anaerobic bacteria for hydrolysis at temperature from room temperature to 37 deg.c. The present invention has the features of fast propagation of hydrolysis bacteria, high adaptability, etc. and is suitable for treatment of various kinds of printing and dyeing waste water.

A method for controlling the pollution of As to soil and water features that the pteridophytes able to collect As, such as Pteris oshimensis, P. fauriei, P. aspericaulis and P. multifida, are planted in the soil or water to be controlled for absorbing the As from soil and water, and their plant stems on the ground are periodically harvested.

The invention provides an efficient production method for cold-pressed recombined bamboo timber. The efficient production method comprises the following technological steps that 1, bamboo splits are dipped in modified low-temperature curing phenol glue; 2, charging and steam-injection for pre-heating are conducted; 3, die pressing is conducted; 4, slabs are heated and cured in a tunnel kiln. The efficient production method has the beneficial effects that that the manufactured cold-pressed recombined bamboo timber can shorten heating time, and the hot pressing efficiency of the recombined bamboo timber is greatly improved; besides, the density of the recombined bamboo timber is lowered by over 10%, and the internal stress of the recombined bamboo timber is lowered; the release time of the internal stress is shortened or release of the internal stress is not needed; during cold pressing, the pressure is only three fourths of conventional pressure, and wire skipping, cracking and other defects of finished products will be reduced; and the quality of the cold-pressed recombined bamboo timber is improved, and therefore the cold-pressed recombined bamboo timber can better meet market demands.

The invention discloses a broad-spectrum composite microbial fungicide and a preparation method thereof. Original composite conidial powder consists of the following three microorganisms: bacillusthuringiensis GSICC32807, bacillus amyloliquefaciens GSICC32826 and pythiumoligandrum GSICC61615. Two types of bacterial spore powder and a type of fungal spore powder are mixed according to the weight ratio of 1:1:1 to prepare a broad-spectrum composite microbial fungicide product. The broad-spectrum composite microbial fungicide product provided by the invention can be quickly planted around the root of a plant to obviously prevent, resist and kill microbial diseases of various crops by competing infection sites with pathogenic bacteria, implementing the hyperparasitism action, secreting auxin, promoting nutrient absorption of the plant and the like; furthermore, the road-spectrum composite microbial fungicide has the effects of promoting the growth of the crops, increasing the yield and improving the quality.

The invention discloses a method for preparing protein feed and a protein feed additive by culturing fly maggots with a defined medium. The fly maggot protein feed is prepared through the steps that the defined medium is inoculated with cyclorrhapha flyblows, culturing is performed for 2-6 days, and then drying is performed; the defined medium is prepared from wheat bran, corn flour, soybean meal and plant fiber powder. According to the method, the fly maggots cultured by the defined medium are adopted as raw materials for preparing the protein feed and the protein feed additive, and therefore continuous breeding all year round can be achieved; the raw materials of the medium are all selected from agricultural leftovers and are high in yield and low in cost, and therefore long-term breeding can be achieved; the defined medium used in the formula is comprehensive and balanced in nutrition and meets the all-aspect fly maggot growth requirements that carbohydrate and an appropriate amount of grease are supplied to fly maggot growth, and maggot protein obtained through culture is beneficial for digestion and absorption of livestock and aquatic animals.

The invention belongs to the technical field of culture and particularly relates to a method for breeding a new local stress-resistant meat rabbit species. According to the method, Sichuan white rabbits and German synthetic white rabbits are taken as breeding materials, and the new local stress-resistant meat rabbit species is bred through a grading hybridization method. The new meat rabbit species bred through the method has the advantages of the Sichuan white rabbits and the advantages of the German synthetic white rabbits and is strong in stress resistance, and the fattening survival rate reaches over 98%; bucks are strong in sexual desire and high in semen quality; does are obvious in estrus and easy in mating, the blood-nest mating pregnancy ratio reaches up to 82%, the number of annual production births is over 7-8 under the condition of artificially-assisted mating, the continuous production performance is good, and the new meat rabbit species has very strong adaptability to the climate environment and culture characteristics of the southwest of China.

The invention provides a pure cotton sanitary pad which comprises a surface layer, an absorption core body and an anti-seepage layer. The surface layer is an absorbent cotton cloth surface layer. The absorption core body is antibacterial pure cotton wool wrapped by pure cotton non-woven fabric. The anti-seepage bottom layer comprises a PE membrane on the upper layer and pure cotton non-woven fabric on the lower layer. The surface layer, the absorption core body and the anti-seepage layer are pressed together. The pure cotton sanitary pad is good in skin fitting performance, and has the advantages of being high in water-absorbing quality and durable in antibacterial performance.

The invention relates to an agricultural livestock breeding technology, in particular to a sow feeding method for increasing the survival rate of piglets in the suckling period. The method includes the steps that after delivery of sows, the sows are fed with compound feed 3 times each day at the time interval of 4 hours or longer; the sows are fed with 2.5 kg of compound feed on the first day after delivery, the feeding amount is increased by at least 0.5 kg each day afterwards and is increased by at least 1 kg each day from the sixth day, and the feeding amount reaches 6.5 kg-7.5 kg on the eighth day and is kept till the piglets are weaned on the twenty-fifth day; from the first day to the twenty-fifth day after delivery of the sows, the sows drink water freely. The compound feed comprises corn flour, peeled soybean meal, imported fishmeal, tapioca flour, sweet potato leaf flour, microbial addictives, calcium hydrophosphate, plasma protein powder, astragalus polysaccharide, bentonite and premix. The feeding method is scientific and reasonable; in combination with the compound feed, in the suckling period, the sows grow healthily, and the survival rate of the piglets is obviously increased.

The invention provides an artificial breeding production method of trichogramma japonicum. The method is high in production efficiency, short in breeding cycle and beneficial to large-scale production of the richogramma japonicum. The method comprises the following steps that 1, sticky eggs are produced; 2, the sticky eggs are dissolved into single particles from an oviposition medium with a neutral protease solution with the concentration being 0.2%-0.5% (g / ml); 3, the sticky eggs treated in the step 2 are used for breeding the trichogramma japonicum. According to the method, the trichogramma japonicum bred artificially is used for preventing and treating agricultural insects, and the agricultural insects mainly comprise rice-stem borer, tryporyza incertulas and rice leaf folder. Compared with the prior art, the method has the advantages that armyworms are simple in source and easy and convenient to breed; the method is high in production efficiency and short in breeding period, large-scale breeding is convenient, and large-scale production of the trichogramma japonicum is promoted.

The invention relates to a selective breeding and propagating method of a plant, particularly relates to a selective breeding and propagating method of a capsicum, belonging to the technical field of plant breeding. Compared with the existing selective breeding technology of a male sterile line of a capsicum, the method has the advantages that the breeding procedure is simple and convenient, the breeding target character and procedure are controllable, the sterile plant rate and the sterile degree are high, and the breeding efficiency is enhanced. A new approach is developed for capsicum heterosis utilization, selective breeding of a leaf color marked sterile line, rapid purity identification of hybrid F1-generation seeds, and the like.

The invention discloses a method of adopting biotechnology to produce admiralty fuel oil, taking oil slurry as raw materials. According to the method, microbial agent composed of activated thermophilic denitriding bacillus NG80-2 (Geobacillus thermodenitrificans) and Mycobacterium goodiisp at the ratio of 1:1 is reacted with high-kinematic viscosity oil slurry in an inorganic salt solution to obtain the No. 380 admiralty fuel oil. A reaction system comprises 1-5%t of activated mixed agents, 50% of oil slurry and the balance of inorganic salt solution. The production method has the advantages of simple process, simplicity in equipment, low cost and environment protection, can produce fuel oil with good quality, and has better processing effect for the oil slurry; and the produced oil product can completely meet the standard of the No. 380 admiralty fuel oil, and has better application prospect.