Multi-strain microorganism pig feed additive and production method thereof
A pig feed additive and microbial technology, applied in the field of multi-strain microbial pig feed additives, can solve the problems of drug resistance of pathogenic bacteria, lower quality of livestock products, high cost of use, etc., and achieve the effects of easy reproduction, improved disease resistance, and clear efficacy
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Embodiment 1
[0025] Example 1 A multi-strain microbial feed additive is inoculated into a fermentation medium according to the ratio of Bacillus subtilis, Lactobacillus delbrueckii, Saccharomyces cerevisiae, Candida and photosynthetic bacteria in a ratio of 2:1:1:1:2, obtained after fermentation.
[0026] The culture medium and culture conditions of Bacillus subtilis are: take 10g of peptone, 3g of beef extract, 5g of NaCl, and 20g of agar powder, add 1000ml of distilled water after mixing, adjust the pH value to 7.0-7.2, and sterilize at 121°C for 20min; culture conditions: The culture temperature of the seed solution was 30°C, pH 7.0, the rotating speed of the shaker was 220 r / min, the filling volume was 50%, the inoculum volume was 4%, and the culture was carried out for 24 hours.
[0027] The culture medium and culture conditions of Lactobacillus delbrueckii are: take beef extract 10g, peptone 10g, yeast extract 5g, glucose 20g, Tween 80lml, anhydrous sodium acetate 5g, dipotassium h...
Embodiment 2
[0032] Example 2 A method for producing microbial feed additives by multi-strain mixed culture. Lactobacillus delbrueckii, Saccharomyces cerevisiae, Candida, and photosynthetic bacteria are inoculated into the fermentation medium at a ratio of 2:1:1:1:2, and the finished product is obtained after solid fermentation.
[0033] The medium and culture conditions during solid fermentation of Bacillus subtilis: take 10g of peptone, 3g of beef extract, 5g of NaCl, and 20g of agar powder, add 1000ml of distilled water after mixing, adjust the pH value to 7.0-7.2, and sterilize at 121 ° C for 20min; Conditions: the culture temperature of the seed liquid is 30℃, pH 7.0, the speed of the shaker is 220r / min, the liquid filling volume is 50%, the inoculum volume is 4%, and the culture is 24h;
[0034] Medium and culture conditions during solid fermentation of Lactobacillus delbrueckii: take beef extract 10g, peptone 10g, yeast extract 5g, glucose 20g, Tween 80lml, anhydrous sodium acetat...
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