Broad-spectrum composite microbial fungicide and preparation method thereof

A technology of compound microorganisms and fungicides, applied in botany equipment and methods, fungicides, biocides, etc., can solve problems such as aggravated environmental pollution, frequent occurrence of human and animal poisoning, and enhanced resistance of pests, and achieve a wide range of hosts and easy access. Reproduction, the effect of promoting the growth of crops

Active Publication Date: 2014-03-26
甘肃绿能农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a broad-spectrum compound microbial fungicide to solve the problems that the existing fungicides make the environmental pollution worse, the poisoning of humans and animals occurs frequently, and the insecticide resistance of pests is enhanced.

Method used

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  • Broad-spectrum composite microbial fungicide and preparation method thereof

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Comparison scheme
Effect test

Embodiment 1

[0037] 1) Preparation of two bacterial spore powders

[0038] The activated Bacillus thuringiensis and Bacillus amyloliquefaciens slant culture strains were respectively inoculated into 500mL primary liquid seed medium according to the inoculation amount of 5%, cultured on a shaker at 28-30°C and 180 rpm, Ferment for 2 days. Then transferred to the second-stage seed shaker flask, cultured on a shaker at 28-30°C with a rotation speed of 180 rpm, and fermented for 2 days. Transfer to 50L bacterial fermenter culture medium again, temperature 28-30 ℃, rotating speed 180 rpm, ferment for 2 days. Transfer the strains of the culture medium of the bacterial fermenter to a 500L production tank at a temperature of 28-30°C and a rotation speed of 180 rpm, and ferment for 3 days to obtain a liquid bacterial suspension. The two kinds of bacterial suspensions are concentrated and dried at low temperature to prepare spore powder. Bacillus thuringiensis contains 10 billion effective viable ...

Embodiment 2

[0044] 1) Preparation of two bacterial spore powders

[0045] Two activated slant culture strains of Bacillus thuringiensis and Bacillus amyloliquefaciens were respectively inoculated into 500mL primary liquid seed medium according to the inoculum amount of 8%, cultured on a shaker at 28-30°C and 180 rpm, Ferment for 2 days. Then transferred to the second-stage seed shaker flask, cultured on a shaker at 28-30°C with a rotation speed of 180 rpm, and fermented for 2 days. Transfer to 50L bacterial fermenter culture medium again, temperature 28-30 ℃, rotating speed 180 rpm, ferment for 2 days. Transfer the strains of the culture medium of the bacterial fermenter to a 500L production tank at a temperature of 28-30°C and a rotation speed of 180 rpm, and ferment for 3 days to obtain a liquid bacterial suspension. The two kinds of bacterial suspensions are concentrated and dried at low temperature, and then prepared into spore powder. Bacillus thuringiensis contains 11 billion effe...

Embodiment 3

[0051] 1) Preparation of two bacterial spore powders

[0052] The activated Bacillus thuringiensis and Bacillus amyloliquefaciens slant culture strains were respectively inoculated into 500mL primary liquid seed medium according to the inoculation amount of 10%, cultured on a shaker at 28-30°C and 180 rpm, Ferment for 2 days. Then transferred to the second-stage seed shaker flask, cultured on a shaker at 28-30°C with a rotation speed of 180 rpm, and fermented for 2 days. Transfer to 50L bacterial fermenter culture medium again, temperature 28-30 ℃, rotating speed 180 rpm, ferment for 2 days. Transfer the strains of the culture medium of the bacterial fermenter to a 500L production tank at a temperature of 28-30°C and a rotation speed of 180 rpm, and ferment for 3 days to obtain a liquid bacterial suspension. The two bacterial suspensions were concentrated and dried at low temperature to prepare spore powder. Bacillus thuringiensis contained 11.3 billion viable bacteria / g; Ba...

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Abstract

The invention discloses a broad-spectrum composite microbial fungicide and a preparation method thereof. Original composite conidial powder consists of the following three microorganisms: bacillusthuringiensis GSICC32807, bacillus amyloliquefaciens GSICC32826 and pythiumoligandrum GSICC61615. Two types of bacterial spore powder and a type of fungal spore powder are mixed according to the weight ratio of 1:1:1 to prepare a broad-spectrum composite microbial fungicide product. The broad-spectrum composite microbial fungicide product provided by the invention can be quickly planted around the root of a plant to obviously prevent, resist and kill microbial diseases of various crops by competing infection sites with pathogenic bacteria, implementing the hyperparasitism action, secreting auxin, promoting nutrient absorption of the plant and the like; furthermore, the road-spectrum composite microbial fungicide has the effects of promoting the growth of the crops, increasing the yield and improving the quality.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and relates to a broad-spectrum composite microbial bactericide and a preparation method thereof. Background technique [0002] The global economic losses caused by crop diseases and insect pests are huge. Chemical pesticides have played a huge role in promoting the prevention and control of diseases and insect pests and the development of agricultural production. Enhance pest resistance. Therefore, the development and use of high-efficiency and broad-spectrum environmentally friendly biopesticides is of great significance to the sustainable development of agriculture, the protection of human health and food safety, and the maintenance of ecological environment and biodiversity. In the national medium and long-term scientific and technological development program, "creation of environmentally friendly pesticides and ecological agriculture" is listed as a key area of ​​agricultural d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P1/00A01P3/00
Inventor 周剑平祝英
Owner 甘肃绿能农业科技股份有限公司
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