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A kind of biological fermentation agent for promoting the decomposing of water hyacinth and its preparation method

A technology of biological fermentation agent and water hyacinth, which is applied in the biological field, can solve the problems of breeding bacteria, affecting the environment, and smelling, and achieves the effects of promoting maturity, simple preparation method, and high nutrient content

Active Publication Date: 2016-08-17
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the water content of the water hyacinth can reach more than 90%, if the water hyacinth cannot be pretreated in time, it will rot and smell, which will breed bacteria and seriously affect the surrounding environment.

Method used

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  • A kind of biological fermentation agent for promoting the decomposing of water hyacinth and its preparation method
  • A kind of biological fermentation agent for promoting the decomposing of water hyacinth and its preparation method
  • A kind of biological fermentation agent for promoting the decomposing of water hyacinth and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The weight ratio of the composition in the bio-fermentation agent that promotes water hyacinth to decompose is:

[0039]

[0040] The concrete steps of the method for preparing the biological fermentation agent that promotes the decomposition of water hyacinth are as follows:

[0041] Step 1, prepare a culture medium with a pH value of 6.5, which is composed of 0.7wt% peptone, 1.3wt% glucose, 10wt% starch, 0.8wt% potassium dihydrogen phosphate, 1.0wt% magnesium sulfate and the rest of water.

[0042] Step 2, at first bacterial classification is mixed to make composite microbial agent, the mixed weight ratio of bacterial classification in the composite bacterial agent is Bacillus subtilis: Trichoderma viride: White rot fungus: Nocardia: Micromonas=30%: 10%: 20%: 20%: 20%;

[0043] The composite bacteria were inoculated into the medium at a weight ratio of 2%:98%, and cultured at 36°C for 72 hours to obtain the stock solution of the composite bacteria.

[0044] Step ...

Embodiment 2

[0050] The weight ratio of the composition in the bio-fermentation agent that promotes water hyacinth to decompose is:

[0051]

[0052] The concrete steps of the method for preparing the biological fermentation agent that promotes the decomposition of water hyacinth are as follows:

[0053] Step 1, prepare a culture medium with a pH value of 6.5, and the culture medium is composed of 1.0wt% peptone, 1.5wt% glucose, 15wt% starch, 1.0wt% potassium dihydrogen phosphate, 1.5wt% magnesium sulfate and the rest of water.

[0054] Step 2, at first bacterial classification is mixed to make composite microbial agent, the mixed weight ratio of bacterial classification in the composite bacterial agent is Bacillus subtilis: Trichoderma viride: White rot fungus: Nocardia: Micromonas=30%: 10%: 20%: 20%: 20%;

[0055] The composite bacteria were inoculated into the culture medium at a weight ratio of 5%:95%, and cultured at 36°C for 72 hours to obtain a composite bacterial agent stock so...

Embodiment 3

[0062] The weight ratio of the composition in the bio-fermentation agent that promotes water hyacinth to decompose is:

[0063]

[0064] The concrete steps of the method for preparing the biological fermentation agent that promotes the decomposition of water hyacinth are as follows:

[0065] Step 1, prepare a culture medium with a pH value of 6.8, which is composed of 0.8wt% peptone, 1.2wt% glucose, 18wt% starch, 0.9wt% potassium dihydrogen phosphate, 0.9wt% magnesium sulfate and the rest of water.

[0066] Step 2, at first bacterial classification is mixed to make composite microbial agent, the mixed weight ratio of bacterial classification in the composite bacterial agent is Bacillus subtilis: Trichoderma viride: White rot fungus: Nocardia: Micromonas=30%: 10%: 20%: 20%: 20%;

[0067] Inoculate the composite bacterial species into the culture medium at a weight ratio of 4%:96%, and culture at 36°C for 72 hours to obtain the stock solution of the composite bacterial agent...

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Abstract

The invention discloses a bio-fermentation agent for promoting water hyacinth decomposition. The bio-fermentation agent comprises a compound bacteria agent, nutritional ingredients, soluble carriers and a microelement chelate, wherein the compound bacteria agent comprises two or more of bacillus subtilis, trichoderma viride, phanerochaete chrysosprium, Nocardia and micromonospora. The invention also discloses a preparation method for the bio-fermentation agent. The preparation method comprises the following steps: preparing compound bacteria species, culturing the compound bacteria species to obtain the compound bacteria agent, and finally, mixing the obtained the compound bacteria agent, the nutritional ingredients, the soluble carriers and the microelement chelate in proportion to obtain the required bio-fermentation agent. The fermentation agent can be manufactured to powder and be dissolved in water completely, a use method is simple, and the propagation of pathogenicity microorganism and incubation of ova can be inhibited to prevent the pest and disease damages; after the fermentation agent is mixed with water hyacinth residual bodies, the decomposition of the water hyacinth residual bodies can be obviously promoted and nutrient content is improved. The method is simple and easy to operate, and low in manufacturing cost.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biological fermentation agent capable of promoting water hyacinth decomposing and a preparation method thereof. Background technique [0002] Water hyacinth is an alien species, widely distributed in Guangdong, Yunnan, Jiangsu, Zhejiang, Sichuan, Hunan, Hubei, Fujian and other provinces in my country because of its strong ecological adaptability, strong reproductive ability, and strong transmission ability. Continue to rapidly spread and spread to surrounding areas. Some important waters in my country, such as Dianchi Lake, Taihu Lake, Wuhan East Lake, the Yangtze River and the Pearl River and the Huangpu River, have been flooded with water hyacinth. Water hyacinth grows wildly after absorbing nitrogen and phosphorus nutrients in river water, which has seriously affected river navigation. [0003] However, water hyacinth itself is a plant rich in protein, amino acid, carotene and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/20C05F17/00C12R1/125C12R1/885C12R1/645C12R1/365
CPCY02W30/40
Inventor 江瀚孟凡清
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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