Atmospheric soil-based immobilized composite microbial agent for improving pepper continuous cropping obstacles and preparation method thereof
A compound microbial inoculant and continuous cropping obstacle technology, applied in the field of crop cultivation, can solve problems such as the decline of pepper yield and quality, lack of solutions or approaches, deterioration of soil physical and chemical properties, etc. The effect of regulating soil fertility and enhancing plant disease resistance
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Embodiment 1
[0028] 1) Slope culture, shaker culture and fermentation culture are carried out on Bacillus subtilis, Bacillus lateralosporus and Bacillus licheniformis respectively, and the mixed fermentation liquid is made according to the proportion of live bacteria of about 3:2:1;
[0029] 2) Mix and ferment attapulgite and compound microbial agent at a ratio of 5:1 for 12 to 24 hours to allow microorganisms to absorb and form a film and dry to obtain attapulgite-immobilized composite microorganisms;
[0030] 3) Sterilize honeycomb slag with a particle size of 1mm, decomposed cow dung and humic acid powder;
[0031] 4) Mix the attapulgite-immobilized microorganisms with honeycomb cinder, decomposed cow dung and humic acid according to the ratio of 50:20:25:5, dry, and the total number of viable bacteria is greater than 10.0×10 8 cfu / g.
Embodiment 2
[0033] 1) Slope culture, shaker culture and fermentation culture are carried out on Bacillus subtilis, Bacillus lateralosporus and Bacillus licheniformis respectively, and the mixed fermentation liquid is made according to the proportion of live bacteria of about 3:2:1;
[0034] 2) Mix and ferment attapulgite and compound bacterial agent at 8:1 for 12-24 hours, make the microorganisms absorb and form a film, and dry to obtain attapulgite-immobilized composite microorganisms;
[0035] 3) Sterilize honeycomb slag with a particle size of 1mm, decomposed cow dung and humic acid powder;
[0036] 4) Mix the attapulgite-immobilized microorganisms with honeycomb cinder, decomposed cow dung and humic acid according to the ratio of 45:15:25:15, and dry them to obtain a composite microbial agent with a total number of viable bacteria greater than 10.0×10 8 cfu / g.
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